[Histonet] Please remove my name
Hi, please remove my name from the mailing list. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FISH
Hi everyone, I was wondering if anyone would like to share their protocol for FISH on PFA fixed tissues? Any help would be hugely appreciated. Thanks so much. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] stains for visualizing new bone growth
You could do a modified tetrachrome stain, this distinguishes newly woven bone. On 2/1/11 2:44 PM, Robin Dean robin_d...@compbio.com wrote: Does anyone know of a good stain to use to clearly show new bone growth other than von Kossa stain? Would appreciate any suggestions anyone might have. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] positive slides
We use VWR Superfrost Slides Cat # 48311-703 and they are great, no lifting sections. On 12/1/10 1:57 PM, zodia...@comcast.net zodia...@comcast.net wrote: Hello, Can anyone recommend a brand of positive slides that are good for IHC. We are having problems with tissue falling off of the slides. Thank You Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biocare¹s Rodent Decloaking Solution
Hi everyone, For anyone that uses Biocare's Rodent Decloaking Solution, once the solution has been made up, can it be reused? And if so in your experience how many times? Many thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone IHC
Hi everyone, I was wondering if anyone has any tricks on how to get bone sections to stop lifting off the slide through the IHC process? I leave them in the oven for quite a while to make sure they are baked on, however after antigen retrieval (pressure cooker for 20mins) most of the boney part of the tissue comes off and the marrow and muscle stays put! The sections are cut onto superfrost plus slides. Any help would be much appreciated, thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Osteoclast
You can do TRAP ( Tartrate-resistant Acidic Phosphatase) stain for osteoclasts. On 10/5/10 4:58 PM, Lin Bustamante lbustama...@cvm.tamu.edu wrote: We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laboratory move
Hi everyone, I was wondering if anyone may have a laboratory relocation guidelines/checklist? Thanks so much ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TUNEL
Hi everyone, I am hoping you can help me I am looking for some advice on how to do TUNEL staining (fluorescent or enzymatic), kits recommended/ protocol. Any help would be greatly appreciated. Thanking you in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Commercial Eosin
Hi everyone, Has anyone had the experience of massive overstaining with commercial Surgipath Alcohol-based Eosin? I manually stained with Surgipath Hx for 2mins, then bluer the eosin for 30secs and the whole tissue was completely pink! Thanks V ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IF
Hi everyone, When carrying out IF on frozen sections how long after the cutting the sections should you leave the slides to dry and where before starting the staining procedure? Thanks a mill, V ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IF on frozen sections
Hi everyone, Does anyone have any experience in IF on mouse embryo frozen sections? Vanessa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Section thickness
Hi Guys, Just wondering what thickness you cut sections at? I was always used to cutting at 2-3 microns in my last lab, however in my new place they are cutting at 6 microns (for both H Es and IHC), which seems to me as really quite thick! What would be the average cutting thickness? Thanks a mill, Vanessa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Section thickness
Ammunition...no, was more just inquiring what the what the average would be and if there was any specific reason for thinner sections other that just preference. Thanks Vanessa On 3/3/09 9:37 AM, Rene J Buesa rjbu...@yahoo.com wrote: Vanessa: Lymph nodes for cellular details (special request) = 3 µm HE and all other special procedures = 5 µm Sections for bone marrow and liver reticulum stain = 7 µm Brain and central nervous system = 10 µm Now a question, why do you want to know this? To have ammunition to challenge what is done in your new lab? Not a wise move. I don't think that they would mind if you cut thinner, but they will mind if you start bringing this issue about. Let your thinner sections speak for themselves. It will get the moment that by example your way will prevail. René J. --- On Tue, 3/3/09, Vanessa J. Phelan vjp2...@columbia.edu wrote: From: Vanessa J. Phelan vjp2...@columbia.edu Subject: [Histonet] Section thickness To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 3, 2009, 9:14 AM Hi Guys, Just wondering what thickness you cut sections at? I was always used to cutting at 2-3 microns in my last lab, however in my new place they are cutting at 6 microns (for both H Es and IHC), which seems to me as really quite thick! What would be the average cutting thickness? Thanks a mill, Vanessa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ideal fixation of mouse prostate
Hi everyone, I am very new to histonet, actually just joined.I hope you guys can help. I was wondering if anyone has any experience in the fixing and processing of mouse issues, namely prostate or bladder. I am currently trying to optomize protocols for this as I have very recently taken on the position of histology manager in a research lab, before this I worked with human samples. We have the VIP processor, and the protocol being followed at the mo is, fixation overnight (though the tissues are tiny) and then washed in PBS and then into 25% alcohol (sometimes over night) and then into the processor, the cycle starts with alcohol instead of formalin. I fear that the tissues are being overfixed and also does anyone leave their tissues in 25% alcohol before processing? Thanking you in advance for any input, Nessa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
