Re: [Histonet] certified/uncertified

[Whitaker, Bonnie via Histonet]

Hi Nancy,

If someone is certification eligible, we hire them into the same position (for 
us it's a C2 level Histotechnologist) and they have one year to get certified. 
The pay would be the bottom off the range, with a raise when they obtain their 
certification.  If they don't have the requisite experience, we hire them in as 
a C1.

Bonnie Whitaker
Ohio State University
AP Operations Director

614-293-8418

-Original Message-
From: Nancy Schmitt via Histonet  
Sent: Wednesday, March 27, 2024 3:58 PM
To: Histonet 
Subject: [Histonet] certified/uncertified

Hello-
Looking for current practices with hiring:
if you were to hire a person with BS that has already been trained in histology 
but is not certified - would they be hired at same rate as certified tech?
would they be expected to get certified?
It's a slippery slope...
Thanks
Nancy Schmitt HT, MLT(ASCP)


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Re: [Histonet] Biopsy formalin fixation times

[Whitaker, Bonnie via Histonet]

But with that, you can consider formalin time after grossing, and on p;rocessor 
to equal those times.

Bonnie Whitaker
AP Operations Director

Office: 614-293-8418
Mobile: 614-313-0484

-Original Message-
From: Thomas Podawiltz via Histonet  
Sent: Wednesday, June 14, 2023 11:18 AM
To: Verno, Victoria R. ; 
Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Biopsy formalin fixation times

Too general of a question. What type of biopsy? Minimum of 6 hours, maximum of 
72 hours for breast. 


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On Wednesday, June 14, 2023, 11:03 AM, Verno, Victoria R. via Histonet 
 wrote:

Good morning everyone,
        I have a question on biopsy formalin fixation time. How long should we 
let the biopsies set and fix in formalin before we gross/process them? You 
response is greatly appreciated.

Victoria Verno HT ASCP
Histology Supervisor of Hampton Roads
Desk: 757-398-4763
Fax: 757-397-7475

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Re: [Histonet] Question about 72 hour upper fixation time for HER2

[Whitaker, Bonnie via Histonet]

Well, I would worry much less about over-fixation than I would under-fixed 
tissues.  Do you not have the option to have a weekend processing schedule that 
runs RT formalin?

Is your volume sufficient you could do an analysis of weekend vs non-weekend 
ER, PR, HER2 rates and see if they are statistically different?  You should be 
keeping the overall statistics already.  If they are not statistically 
different, that should ease your mind,

Good luck!
Bonnie Whitaker




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From: Tony Auge via Histonet 
Sent: Friday, May 5, 2023 6:50:00 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Question about 72 hour upper fixation time for HER2

Hi Histonet,

Does anyone know if the 72 hour upper fixation time for HER2 was calculated
at room temperature? My lab is currently running our formalin at 40°c and
we go 72 hours on the long weekends, so I'm worried it might be overfixed.

Thanks,
Tony

--

Tony Auge HTL (ASCP)CM QIHC
Cell: (651) 373-4768
Email: tony.a...@gmail.com
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Re: [Histonet] Tissue Processor Schedule Validations

[Whitaker, Bonnie via Histonet]

Put in two cassettes of resection tissue that are cut to mimic biopsy 
sizes/tissue (colon resection for colon bx, liver resection for liver bx, etc.

Bonnie Whitaker
Ohio State University
AP Operations Director

614-293-8418

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 2:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Expired Tissue Blocks

[Whitaker, Bonnie via Histonet]

Caution to all--make sure your patients have signed consent forms that will 
include this use of their tissue.

Bonnie Whitaker
Ohio State University
AP Operations Director

614-293-8418

-Original Message-
From: Ken M via Histonet  
Sent: Thursday, January 12, 2023 3:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Expired Tissue Blocks

External Email: CAUTION

Don't throw your expired embedded blocks in the trash. Some of them can be very 
valuable for research and antibody development. If you can provide a brief 
description of any positive block (no patient identifiers or names please), we 
will pay you top dollar to take them and send you all shipping labels and 
documents ahead of time so you can send your entire storage unit to us. We can 
provide certification that we will properly dispose of and be responsible for 
the blocks in our possession if our research partners find them of no use. For 
some specific positive tissues, a single block blocks can be worth over a 
hundred dollars to our partners.

Please contact us at contac...@springsidesci.com if interested.


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Re: [Histonet] BLOCKS AND SLIDES

[Whitaker, Bonnie via Histonet]

It depends on who your most stringent governing body is.  If you have a lab in 
NY, or that takes specimens from NY, it's 20 years for both.  For CAP it's 10 
years.  There may be other states that have more stringent rules, etc.

Bonnie Whitaker
Ohio State University
AP Operations Director

614-293-8418

-Original Message-
From: Dorothy Glass via Histonet  
Sent: Thursday, January 12, 2023 1:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] BLOCKS AND SLIDES

External Email: CAUTION

What is the required length of time that you must keep blocks and slides in 
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Re: [Histonet] Technical/Performing

[Whitaker, Bonnie via Histonet]

We use the lab location in the header, and the pathologists have different log 
ones that list where they did the professional below their electronic signature.

Get Outlook for iOS

From: Abbott, Tanya via Histonet 
Sent: Wednesday, May 4, 2022 6:47:09 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Technical/Performing

External Email: CAUTION

We currently have a "performing location" listed on our reports. CAP is telling 
me that if you have technical work at one lab, and sign out/Interp at another 
lab, you must list both on the report.
We are a multiple facility where my lab does our own work, but will be 
performing technical work for another hospital in our health system. I also 
have a bigger hospital that will be doing this for different facilities. So 5 
facilities in total.
Thoughts on how to handle this? We are thinking a generic disclaimer but it's 
going to be lengthy! Hope my explanation made sense?!

Tanya G. Abbott, HT (ASCP)CM, MLT(CSMLS)
Pathology Manager

Penn State Health St. Joseph
Pathology Department
(o) 610-378-2635
(f) 610-898-5871
tabb...@pennstatehealth.psu.edu
Penn State Health St. Joseph | The Future of 
Healthcare


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[Histonet] Ohio State University job openings

[Whitaker, Bonnie via Histonet]

Hi All,

We currently have an Evening/Night Supervisor position open, in the OSU 
Clinical Histology Lab.  The hours are generally 4:30 PM-1:00 AM.

If you are interested, please email Robyn Mulhall with your resume'.  Her email 
address is robyn.mulh...@osumc.edu.

Thanks!
Bonnie

Bonnie Whitaker, MT(HEW), HT(ASCP)QIHC
Anatomic Pathology Operations Director

The Ohio State University Wexner Medical Center
Department of Pathology
Suite 6100
Optometry Clinic & Health Sciences Faculty Building
241 11th Avenue
Columbus, OH  43210

6 14.293.8418

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[Histonet] slide printer

[Whitaker, Bonnie via Histonet]

Hi All,

I am posting this for a friend.  Any info would be appreciated.

Any way we are looking at several slide printer options and wondered the 
following:

1)  What are you using?

2)  Did you first try any other vendors besides the one you are using now?

3)  Do you like what you are using now?

4)  Is it a one or two slide hopper - ie can you load and then select 
charged or non-charged slides?

5)  Are you using vendor specific recommended slides or have you 
substituted for a cheaper or other option?

6)  We are looking specifically at Leica, SlideMate and ESPO - do you have 
any experience of info from other users you know about these vendors?

Any and all information you are able to share would be appreciated.


Thanks,
Bonnie

Bonnie Whitaker
AP Operations Director
The Ohio State University
Department of Pathology

614-293-8418


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Re: [Histonet] Distilled Water vs DI water for IHC and H staining

[Whitaker, Bonnie via Histonet]

Hi Charles,

You will need to give it a try, in order to know for sure.

Water quality is EXTREMELY variable, from community to community, and even the 
pipes, etc., within in your building can influence the water quality.

The chemicals used by the local water district can also vary, based upon the 
season, and your geographical location.

Good luck!

Bonnie



Sent with Good (www.good.com)
Bonnie

-Original Message-
From: Charles Riley via Histonet 
[histonet@lists.utsouthwestern.edu]
Sent: Thursday, July 28, 2016 07:54 AM Eastern Standard Time
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Distilled Water vs DI water for IHC and H staining


Does anyone know if there is a difference in staining results when using DI
water versus Distilled water? Our lab is trying to get away from the DI
system we have as it is becoming expensive to maintain and we were looking
to see if we could just use a home drinking filter with UV lighting for our
water supply. Please let me know any thoughts or concerns you might have
with this idea

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] CAP checklikst for pathology lab

[Whitaker, Bonnie]

The checklists are now customized, and your version must be downloaded from 
CAP.org.

Good luck!

Bonnie



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-Original Message-
From: Пешков Максим [maxim...@mail.rumailto:maxim...@mail.ru]
Sent: Monday, June 29, 2015 11:43 PM Eastern Standard Time
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP checklikst for pathology lab


Dear histonetters, can you send me, pleasse, the last pdf version cap checklist 
for pathology lab? Or reference where I can download it.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
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Re: [Histonet] Specimen Accessioning .....

[Whitaker, Bonnie]

Ditto for me... most, if not all, of the places I've worked.
Bonnie



-Original Message-
From: Weems, Joyce K. [mailto:joyce.we...@emoryhealthcare.org] 
Sent: Wednesday, June 03, 2015 4:14 PM
To: 'Cartun, Richard'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Specimen Accessioning .

We do A, B, C, etc.. for the specimens.

And then do A1, A2, A3, B1, B2, B3, etc... for the blocks. Took be a while to 
get them to change to that, but it is what makes sense to me... j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.


-Original Message-
From: Cartun, Richard [mailto:richard.car...@hhchealth.org]
Sent: Wednesday, June 03, 2015 3:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Specimen Accessioning .

We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and 
the corresponding paraffin blocks as A, B, C, etc.  We are in the process of 
changing this to letters for the different specimen parts and numbers for the 
blocks.  For those of you who do it this way, have you encountered any 
problems?  For example, if the specimen arrives and the different parts are 
numbered, do you simply convert them to letters?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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Re: [Histonet] Co-path users logging Slides in

[Whitaker, Bonnie]

Ditto.

-Original Message-
From: Weems, Joyce K. [mailto:joyce.we...@emoryhealthcare.org] 
Sent: Tuesday, May 19, 2015 5:10 PM
To: Histology Technician; Histonet; histonet-requ...@lists.utsouthwestern.edu
Subject: Re: [Histonet] Co-path users logging Slides in

We accession them as a case and document what is received in the report.



Joyce Weems

Pathology Manager

678-843-7376 Phone

678-843-7831 Fax

joyce.we...@emoryhealthcare.org







www.saintjosephsatlanta.org

5665 Peachtree Dunwoody Road

Atlanta, GA 30342



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contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.



-Original Message-

From: Histology Technician [mailto:histology81...@att.net]

Sent: Tuesday, May 19, 2015 5:02 PM

To: Histonet; histonet-requ...@lists.utsouthwestern.edu

Subject: [Histonet] Co-path users logging Slides in



  Those of you who use Co-path, how do you log slides from outside hospitals?  
I see where you log slide Send outs, do you log incoming slides the same way?  
I'm trying to figure out how to keep track of my slide consult slides  - when I 
received them, how many I received, when I sent them back to the original 
hospital, etc...

Thanks!

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Re: [Histonet] Tissue transfer method:

[Whitaker, Bonnie]

I have used this for IHC, as well.  It's great stuff!  You can also use other, 
very viscous mounting medias, but the time it takes to dry/soak are longer than 
with Mount-Quick!

Thanks,
Bonnie

-Original Message-
From: Shirley A. Powell [mailto:powell...@mercer.edu] 
Sent: Friday, May 01, 2015 4:27 PM
To: James Watson; 'Jb'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue transfer method:

With Mount Quick you can also decolorize the section and do a different stain 
on them.  I have even performed IHC on these transferred sections when needed.

Shirley Powell

-Original Message-
From: James Watson [mailto:jwat...@gnf.org] 
Sent: Friday, May 01, 2015 4:21 PM
To: 'Jb'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue transfer method:

Slide Repair

1.  Soak coverslip off of slide in xylene.
2.  Piece slide back together.
3.  Apply Mount-Quick to slide, covering tissue. Allow to dry overnight.
4.  Place slide in cold water in refrigerator overnight.
5.  Carefully peal the Mount-Quick off the slide.  The tissue should come 
up with the Mount-Quick, if it does not stop pealing up the Mount-Quick and 
place back into the water overnight again.
6.  Carefully trim excess Mount-Quick from areas where tissue is not 
present. Leave at least ¼ inch of Mount-Quick around the tissue
7.  On a new slide that is wet with water, place the removed Mount-Quick 
and tissue in the center of the slide (make sure the tissue is next to the 
slide), removing as many air bubbles from under the Mount-Quick as possible.
8.  Cover the slide with a wet piece of filter paper then place a blank 
slide on top.  If you have multiple slides to repair then they can be stacked.  
Apply weight to the top of this and let stand overnight.
9.  Soak the dry slide in Xylene until the Mount-Quick is removed and 
coverslip as usual.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific 
Technical Leader II, Histology Tel    858-332-4647 Fax   858-812-1915 
jwat...@gnf.org

-Original Message-
From: Jb [mailto:craiga...@gmail.com]
Sent: Friday, May 01, 2015 1:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue transfer method:

Does anyone have a procedure that they can share on tissue transfer? One 
stained slide to another?

Thank you for your help.

Sincerely,

Craig

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RE: [Histonet] histology in higher education

[Whitaker, Bonnie]

Andi,

Would you be willing to share the information on how to volunteer with this 
program?

Thanks,
Bonnie

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Wednesday, April 22, 2015 11:40 AM
To: 
Subject: RE: [Histonet] histology in higher education

For a few years I've been involved in a program called letters to a 
pre-scientist. The idea is to reach middle schoolers as they are being 
introduced to the sciences. They have pretty high goals at this time, they want 
to be doctors and astronauts and engineers but they are just starting to learn 
about these things.
You become a pen pal/mentor of sorts and write letters to a child and they will 
write back to you. Last year I was writing to a boy in the Chicago area and 
this year it was a girl in LA. I always write about what I do and how important 
it is and include pictures of things like brain cells, muscle, fungus, bacteria 
and pictures of my lab. I always pick up a copy of the NSH coloring book and 
send it to them and tell them what they need to study to be a histotech and 
other than a hospital, where they can find a job. Of course we also tell them 
about other things like our families, pets, vacations, etc. at the same time. 

It's just a small thing but it plants a seed.

Andi G.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of 
wsim...@athensgastro.com [wsim...@athensgastro.com]
Sent: Wednesday, April 22, 2015 7:31 AM
To: koelli...@comcast.net; 
Subject: Re: [Histonet] histology in higher education

Good morning Ray and thank you for promoting the field of Histotechnology.

While President of the NSH, Vinnie DellaSperanza started a career day function 
at the annual NSH symposium.  It has been very successful and the individuals 
that contribute to this volunteer effort are usually the same individuals that 
participate at the state and regional level.
Thank you for the idea of state fairs and other avenues for the target age of 
middle  high school students.
I did this myself when my children were in girl scouts , Odyssey of the Mind 
and advance placement opportunities.


Wanda K. Simons, HT (ASCP)
GSH President
www.histosearch.com/gsh/


  ---Original Message---
  From: koelli...@comcast.net
  To: histonet@lists.utsouthwestern.edu
  Subject: [Histonet] histology in higher education
  Sent: Apr 22 '15 10:14

  The following has to do with histology and STEM (science, technology, 
 engineering, math) so if not interested, please ignore.  But I believe it can 
 have real meaning to the profession of histology at the NSH, state society 
 and local levels.

  I am elected to the Board of WSSEF (Washington State Science and Engineering 
 Fair) where I am in educational outreach and also the assistant to the head 
 judge.  We recently had our Washington State Fair with 650 kids, grades 1-12 
 from all over the state.  And while there was a lot of engineering and robots 
 and computers there were a few projects having to do with medicine, 
 biotechnology, immunology and pathology with some familiar histology or 
 immunohistochemistry pictures included.  At the end of the fair, we awarded 
 almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 
 students.  Not only that, our top winners get an all-expenses paid trip to 
 present at the ISEF (Intel International Fair) with 1,700 students competing 
 from all 50 states and 70 countries.  Wherever you are in the US, you have a 
 state fair.

  I would advocate for some of you so interested at the national, state or 
 local levels to promote histology, by getting involved as mentors for middle 
 and high school students to science fairs; especially those that could lead 
 to histopathology or other related projects that could lead into Intel 
 affiliated fairs resulting in great benefit to the student and a spread of 
 the word of histology into both the STEM world and general population.

  I've mentored for 15 years.  It can be done.  Molecular histopathology, 
 personalized diagnostics and therapeutics, advances in immunohistochemistry, 
 current controversies about breast biopsy diagnosis, or other disease with 
 newer classifications, PCR and RTPCR in histology, modern-targeted 
 therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image 
 analysis software for you computer geeks and on and on; the list is nearly 
 limitless.  Especially if you are close to or can contact biotech companies 
 or educational institutions to find co-mentors for grades 7-12 there are 
 histology-related science project possibilities in terms of data collection 
 and the scientific method and project presentation are nearly unlimited now.

  Be a mentor for or engage a grade 7-12 student, with the help of another 
 mentor or organization, to think about 

[Histonet] RE: bone marrow billing fees

[Whitaker, Bonnie]

Hi Lacie,

Anything that is grossed, embedded and has slides cut and stained by histology 
(core bx or clot) should be billed an 88305.

Bonnie

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A
Sent: Wednesday, March 18, 2015 4:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone marrow billing fees

Hi Everyone,
I was wondering if anyone is billing for a Histo technical charge on bone 
marrow aspirates and biopsies (i.e. an 88305) in addition to all of the 
Hematology fee codes.  Our Histology department does the grossing, embedding 
and microtomy for all bone marrow blocks, however we do not bill for/receive 
any revenue for this work.
Thanks,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.al...@providence.orgmailto:lacie.al...@providence.org


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[Histonet] slide folders for double wide slides

[Whitaker, Bonnie]

Hi All,

What slide folders do any of you recommend for use with 2x3 slides (we use 
them for whole-mount prostates)?  If you have some that work well, could you 
send me the ordering info?

Thanks,
Bonnie

Bonnie Whitaker
AP Operations Director

The Ohio State University
Wexner Medical Center
Department of Pathology
N305 Doan Hall
410 West 10th Avenue
Columbus, Ohio  43210

614.293.8418
FAX 614.293.2779
Pager: 614.293.7243 ext. 5013

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RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta

[Whitaker, Bonnie]

I want to add my thanks to everyone, as well.  This is a service that many of 
us rely on, and appreciate on a daily basis, even if we forget to voice it!


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A
Sent: Friday, December 19, 2014 3:23 PM
To: 'Linda Margraf'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta 

Thank you and all who help you for this service.  It is greatly appreciated and 
needed for all who are in the Histology field.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Margraf
Sent: Friday, December 19, 2014 1:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta 

 Dear Histonetters,
 In a couple of weeks, Histonet will celebrate its 19th anniversary! It is 
 hard to believe it has been in existence that long. I would like to say 
 thanks to all the members who contribute to the list, sharing their time and 
 expertise with others in histology-related fields. We currently have 3800 
 subscribers from at least 30 countries ( last time I counted).  It is amazing 
 to see how the list continues to provide new insights and information after 
 all these years.
 I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr 
 Sandy Cope as a co-administrator of the list with me. Anita is also a 
 pediatric pathologist and she will help with some of the issues with list 
 subscribing etc. Please continue to be patient with us if you are having list 
 issues as we often get busy in our day jobs. If your message to the list 
 doesn't post and it says it is because you are not a member, please see if 
 you need to update your email address on the membership page. Remember you 
 can only post to the list from an email address that matches exactly with one 
 on the subscriber list. You can always send us an email at the 
 histonet-owners address and we will try to help you out. Best wishes for a 
 happy and safe holiday season.
 Linda M
 Histonet administrator 
 
 Sent from my iPhone
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[Histonet] ATRX

[Whitaker, Bonnie]

Hi Everyone,

Does anyone have ATRX available as a clinical test?  I need some that will do 
the technical only for us.

Thanks,
Bonnie

Bonnie Whitaker
AP Operations Director

The Ohio State University
Wexner Medical Center
Department of Pathology
N305 Doan Hall
410 West 10th Avenue
Columbus, Ohio  43210

614.293.8418
FAX 614.293.2779
Pager: 614.293.7243 ext. 5013

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RE: Re[2]: [Histonet] QIHC test

[Whitaker, Bonnie]

The IHC Resource Committee hopes to soon have some IHC materials available via 
the NSH website.  I'll email the group as soon as I learn that it is active.  
You will have to be an NSH member in order to have access to the links.

Thanks,
Bonnie Whitaker

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brendal Finlay 
[brendal.fin...@medicalcenterclinic.com]
Sent: Thursday, September 04, 2014 6:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re[2]: [Histonet] QIHC test

The one I received last year has essential and recommended reading lists on 
page 8.  I am still studying for the qualification.  I've been using the two 
guides from Dako, Carson's Histotechnology A Self-Instructional Text, and the 
elusive Elias' Immunohistopathology A Practical Approach to Diagnosis.  There 
are some Journal of Histotechnology articles I'd like to get my hands on, but 
have not been able to yet.  I'm also curious about the other books.


Brendal C. Finlay, HT (ASCP)

-Original Message-
From: Joelle Weaver joellewea...@hotmail.com
To: Amber McKenzie amber.mcken...@gastrodocs.net, 
histonet@lists.utsouthwestern.edu
Date: 09/03/2014 15:33
Subject: RE: [Histonet] QIHC test

Dako education guides, available from their website. Not answer books, but 
good for reviewing theory. If the MSH one is like their other study guides, the 
learning activity is to look up and fill in the answers from other resources 
materials.


Joelle Weaver MAOM, HTL (ASCP) QIHC





 From: amber.mcken...@gastrodocs.net
 To: histonet@lists.utsouthwestern.edu
 Date: Wed, 3 Sep 2014 14:26:52 +
 Subject: [Histonet] QIHC test

 I ordered the MSH online study guide for the QIHC, but it was only 
 questions...no answer key in the back.  What books am I supposed to get the 
 answers from?  Any other study suggestions? Thanks!

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[Histonet] does anyone do IHC for pepsin clinically?

[Whitaker, Bonnie]

Hi All,

We've had a request from a clinician to perform IHC on a GI biopsy  for pepsin. 
 Does anyone have this up and running for clinical cases, or know of a 
reference lab that performs this?

Thanks!
Bonnie

Bonnie Whitaker
Anatomic Pathology Operations Director
Department of Pathology Division of Anatomic Pathology
N305 Doan Hall, 410 West 10th Avenue, Columbus, OH, 43210
614-293-8418 Office / 614-293-2779 Fax / 614-293-5013 Pager
bonnie.whita...@osumc.edumailto:bonnie.whita...@osumc.edu
We are committed to improving people's lives through personalized health care.




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[Histonet] Histology Manager position

[Whitaker, Bonnie]

Hi Everyone,

The position of Clinical Histology Laboratory Manager here at The Ohio State 
University Medical Center is still open:

We are located in Columbus, OH which is a wonderful city with a population of 
1.75 million. We are the 16th largest city in the US, without the traffic 
problems of some of the larger cities. There are 19 communities within 30 
minutes of downtown Columbus. It really is a lovely area, complete with four 
seasons.

The histology lab is a good lab, with a stable, hard-working staff. Our average 
block count is about 765/day and we have about 18 techs in histology.

Also, we have great benefits!!

Here is the official blurb:

The OSUMC clinical histology laboratory is a functional laboratory within the 
Anatomic Pathology branch serving the pathology subspecialty divisions. The 
Manager will provide leadership for daily operations of the histology labs, 
manages human resources, financial and budget planning and preparation, new 
test development, quality improvement, safety programs, compliance program, 
successful inspection and accreditation processes, and ensuring current 
policies and procedures related to technical, operational, and administrative 
areas of the laboratory.

For a complete position description and application instructions please go to 
http://medicalcenter.osu.edu/careers/ http://medicalcenter.osu.edu/careers/ 
and search by posting number 354362. The Ohio State University is an equal 
opportunity, affirmative active employer. Women, minorities, veterans, and 
individuals with disabilities are encouraged to apply.

Please don't hesitate to give me a call, or email me if you want more 
information.

Thanks!
Bonnie

Bonnie Whitaker
AP Operations Director
The Ohio State University Medical Center
Department of Pathology
614.293.5048


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[Histonet] Ohio State Clinical Histology Manager Position

[Whitaker, Bonnie]

Hi Everyone,

We are still searching for the right person to fill the Clinical Histology 
Manager position at The Ohio State University Medical Center in Columbus, Ohio.

The lab does a brisk volume, our daily block count is around 760.  We are open 
from Sunday evening until Saturday morning, with call rotation for Saturday 
morning through Sunday evening.  It is a great group of people to work with.

The official blurb is:

The OSUMC clinical histology laboratory is a functional laboratory within the 
Anatomic Pathology branch serving the pathology subspecialty divisions; 
Histology Lab Manager will provide leadership for daily operations of the 
histology labs; manages human resources, financial and budget planning and 
preparation, new test development, quality improvement, safety programs, 
compliance program, successful inspection and accreditation processes, and 
ensuring current policies and procedures related to technical, operational, and 
administrative areas of the laboratory are adhered to.

We prefer someone with a solid background in clinical histology, as well as 
supervisory experience.

If you are interested, please send me an email with your current resume/CV.

Thanks!
Bonnie

Bonnie Whitaker

AP Operations Director
The Ohio State University Medical Center
Anatomic Pathology Branch
Department of Pathology
N308B Doan Hall
410 W. 10th Ave.
Columbus, OH  43210

bonnie.whita...@osumc.edu

phone 614.293.5048
fax 614.293.7273
pager 614.346.5013


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[Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA

[Whitaker, Bonnie]

 
The only time a skin is above an 88305 is when it is just the 'covering' on 
something that has soft tissue involvement to the point that it then becomes a 
soft tissue specimen, rather than a skin.

That's my take on it.

Bonnie Whitaker
AP Operations Director
Ohio State University Medical Center
Department of Pathology
614.293.5048
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Thursday, January 13, 2011 12:21 PM
To: Vickroy, Jim; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: CPT CODES FOR RE-EXCISIONS OF MELANOMA

I think a skin is a skin is a skin. We use 88305. j 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Thursday, January 13, 2011 12:03
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA

WHAT IS EVERYONE USING FOR THE  CPT CODES ON RE-EXCISIONS OF MELANOMA FOR 
MARGINS?   IN THE PAST WE HAVE BEEN USING 88307 IF IT WAS A DEEP EXCISION WITH 
NO RESIDUAL TUMOR AND AN 88309 WHEN IT WAS A DEEP EXCISION WITH RESIDUAL TUMOR. 
  WE HAVE NOW BEEN QUESTIONED ON WHETHER ALL SHOULD ACTUALLY BE 88305.

How is everyone else charging these?  I guess the next question is what 
constitutes a deep excision?

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor Memorial Medical Center
217-788-4046



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[Histonet] Histology Lab Manager--Ohio State University Med Center

[Whitaker, Bonnie]

 
Hi Everyone,
 
The position of Clinical Histology Laboratory Manager here at The Ohio State 
University Medical Center is still open:
 
We are located in Columbus, OH which is a wonderful city with a population of 
1.75 million.  We are the 16th largest city in the US, without the traffic 
problems  of some of the larger cities.  There are 19 communities within 30 
minutes of downtown Columbus.  It really is a lovely area, complete with four 
seasons.  

The histology lab is a good lab, with a stable, hard-working staff.  Our 
average block count is about 765/day and we have about 18 techs in histology. 

Also, we have great benefits!!

Here is the official blurb:
 
The OSUMC clinical histology laboratory is a functional laboratory within the 
Anatomic Pathology branch serving the pathology subspecialty divisions.  The 
Manager will provide leadership for daily operations of the histology labs, 
manages human resources, financial and budget planning and preparation, new 
test development, quality improvement, safety programs, compliance program, 
successful inspection and accreditation processes, and ensuring current 
policies and procedures related to technical, operational, and administrative 
areas of the laboratory.  

For a complete position description and application instructions please go to 
http://medicalcenter.osu.edu/careers/ http://medicalcenter.osu.edu/careers/   
and search by posting number 354362. The Ohio State University is an equal 
opportunity, affirmative active employer. Women, minorities, veterans, and 
individuals with disabilities are encouraged to apply. 
 

Please don't hesitate to give me a call, or email me if you want more 
information.
 
Thanks!

Bonnie Whitaker

AP Operations Director
Ohio State University Medical Center
Anatomic Pathology Branch
Department of Pathology
N308B Doan Hall
410 W. 10th Ave.
Columbus, OH  43210

bonnie.whita...@osumc.edu

phone 614.293.5048
fax 614.293.7273
pager 614.346.5013
 


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[Histonet] Histology Lab Manager--Ohio State University Med Center

[Whitaker, Bonnie]

Hi Everyone,
 
We have an opening for the Clinical Histology Laboratory Manager here at The 
Ohio State University Medical Center (this is my position, as I have accepted 
another role within our department starting Dec. 1.)
 
We are located in Columbus, OH which is a wonderful city with a population of 
1.75 million.  We are the 16th largest city in the US, without the traffic 
problems  of some of the larger cities.  There are 19 communities within 30 
minutes of downtown Columbus.  It really is a lovely area, complete with four 
seasons.  

The histology lab is a good lab, with a stable, hard-working staff!
 
Here is the official blurb:
 
The OSUMC clinical histology laboratory is a functional laboratory within the 
Anatomic Pathology branch serving the pathology subspecialty divisions.  The 
Manager will provide leadership for daily operations of the histology labs, 
manages human resources, financial and budget planning and preparation, new 
test development, quality improvement, safety programs, compliance program, 
successful inspection and accreditation processes, and ensuring current 
policies and procedures related to technical, operational, and administrative 
areas of the laboratory.  

For a complete position description and application instructions please go to 
http://medicalcenter.osu.edu/careers/ http://medicalcenter.osu.edu/careers/   
and search by posting number 354362. The Ohio State University is an equal 
opportunity, affirmative active employer. Women, minorities, veterans, and 
individuals with disabilities are encouraged to apply. 
 

Please don't hesitate to give me a call, or email me if you want more 
information.
 
Thanks!

Bonnie Whitaker 
Clinical Histology Manager 
The Ohio State University Medical Center 
Anatomic Pathology Branch 
Department of Pathology 
N308B Doan Hall 
410 W. 10th Ave. 
Columbus, OH  43210 

bonnie.whita...@osumc.edu 

phone 614.293.5048 
fax 614.293.7273 
pager 614.346.5013 

 


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[Histonet] FFPE used for EM

[Whitaker, Bonnie]

Hi Everyone,

I hope it's a great Friday for you all.  

Our renal folks were recently attending a meeting, and they came back with
some partial information.  (I am hoping some of you might have some
additional information.)  It seems that one of the lecturers mentioned that
some paraffins actually seemed to make a difference in the quality of EM when
they had to take tissue from the paraffin blocks.  I had never heard of this.
I am aware of using Carson's modification of  formalin to yield better EM
results, but not anything to do with paraffin.

Can anyone help me out here?

Thanks!

Bonnie Whitaker
Clinical Histology Manager
Ohio State University Medical Center
614.293.5048



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[Histonet] Histotech position in Little Rock

[Whitaker, Bonnie]

Hi Everyone,

I hear that there is a histotech position open at University of Arkansas
Medical Center.  If you are interested, go online and apply!  There are some
great folks in Arkansas!  I'm sure it will be a great opportunity for the
right person.

Bonnie

Bonnie Whitaker
Clinical Histology Manager
Ohio State University Medical Center
614.293.5048



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RE: [Histonet] Block patient IDs

[Whitaker, Bonnie]

Hi Everyone,

Maybe there is something new, but we had our inspection last week, and the
customized checklist used for our inspection does not require 2 identifiers.
It states:

NOTE: An unambiguous system of specimen identification coupled with a
legible, sequential cassette
and slide labeling system that withstands reagents and stains are essential
to fulfill this requirement. The
number of blocks processed and the number of slides prepared for each case
must be recorded.

ANP.21100 Phase II N/A YES NO
Are blocks identified adequately?

NOTE: Each block of tissue must be identified by the entire accession number
assigned to the case and
by any descriptive letter(s)/number(s) added by the prosector during the
dissection. If additional blocks
are prepared later, all lists and logs must reflect these additions.
Identification number and
letter(s)/numbers(s) must be affixed to all blocks in a manner that remains
legible.

ANP.21150 Phase II N/A YES NO
Are slides identified permanently with adequate, legible information?

NOTE: Each slide must be identified by the entire accession number and
descriptive letters unique to the
block from which it is cut. Other appropriate identifiers should be included
as applicable (e.g., levels of
sectioning). Automated prelabeling systems are acceptable. Regardless of
whether the identifying
information is on the slide or on a label, the information must be indelible,
legible and able to withstand
all stages of processing and conditions of storage.

The laboratory director is responsible for ensuring that slides are
adequately, permanently identified. 

I don't know if that helps, but these were copied and pasted directly from
the checklist.  

Bonnie Whitaker
Clinical Histology Manager
Ohio State University Medical Center
614.293.5048


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Brown
Sent: Friday, March 12, 2010 11:00 AM
To: Nails, Felton; Anne van Binsbergen;
histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Block patient IDs

I think  you will find that CAP is asking if 2 patient identifiers are used
throughout the entire process.  We engrave the blocks, slides and a computer
generated block count log that uses 2 patient identifiers.

 

Terri H. Brown,, HT (ASCP)
Pathology Laboratory Manager
Northside Hospital  Atlanta
terri.br...@northside.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton
Sent: Friday, March 12, 2010 10:55 AM
To: 'Anne van Binsbergen'; histonet-requ...@lists.utsouthwestern.edu;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Block patient IDs

It is my understand that the two patient identifier applies to the completed
slide not the block.
You can verify this by looking at the CAP Checklist for anatomical pathology 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne van
Binsbergen
Sent: Friday, March 12, 2010 9:42 AM
To: histonet-requ...@lists.utsouthwestern.edu;
histonet@lists.utsouthwestern.edu
Subject: [Histonet] Block patient IDs

A question for all you CAP fundis: how many patient Identifiers are needed on
each paraffin block

We currently just write the unique, LIS generated number on the block face,
but have recently been advised that CAP and JCIA require not one, but TWO
patient IDS on the block

Comments please

--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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intended recipient, you  are hereby notified that any review, dissemination,
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of it, or 

RE: [Histonet] requirements for certification

[Whitaker, Bonnie]

I agree with Liz.  It seems with a bio major and chem minor, that should take
care of the coursework.  You could do the IU online program, but if you are
self-motivated, I would just study on my own, if I were you.  If you don't
pass the first time, then at least you would know how close you were, and
could decide to take the course, or just to study a little more.  If you can
pass the exam, why waste the time and money unnecessarily?  It sounds like
you probably have a great background and experience already!

Bonnie Whitaker

Clinical Histology Manager
Ohio State University Medical Center
N308B Doan Hall
410 W. 10th Ave.
Columbus, OH  43210

bonnie.whita...@osumc.edu

phone 614.293.5048
fax 614.293.7273
pager 614.346.5013
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Wednesday, January 13, 2010 4:57 PM
To: Jennifer Anderson; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] requirements for certification

You might be eligible to sit for the registry already either HT or HTL, you
just need to have the correct number of credits in biology and chemistry to
meet the requirements.  I would call ASCP to see if you have the
requirements, it seems to me that you have the experience needed you just
need to make sure you have the credits.  That's my opinion.  All you need is
one year on the job training with a BS or BA to sit for the HTL.  I think I'm
correct on this, but if I'm not we'll know soon enough from the replys.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO
Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Anderson
Sent: Wednesday, January 13, 2010 2:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] requirements for certification

Hello.

I'm thinking about getting my HTL certification.  I've been a bench scientist
in both academia and biotech for 21 years, which has included pre-clinical
animal research and subsequent histological applications.
I've set up an histology lab and have a lot of IHC but only basic staining
(mostly HE), as well as loads of animal tissue processing and some human
tissues.  I have a liberal arts degree (an AB) with a biology major and a
chemistry minor.  We have both a DVM and a few MD's on site, but for
pre-clinical and clinical research consult.  I would appreciate any insight
on getting certified through an online program, how many hours is required,
and what kind of mentorship is necessary.

Thank you for your insights and time!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333
jander...@halozyme.commailto:jander...@halozyme.com


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[Histonet] Sunquest/CoPath question

[Whitaker, Bonnie]

Hi All,

Will anyone using Sunquest/CoPath please contact me?  I am trying to improve
the level of accuracy with our Part Types, and I wondered how others are
addressing specimens that arrive en bloc, but can be separated out for
billing purposes.

Thanks, and Happy Holidays!

Bonnie Whitaker
Clinical Histology Manager
Ohio State University Medical Center
614.293.5048

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[Histonet] Workload value of whole mount embedding and cutting

[Whitaker, Bonnie]

Hi Histonetters,

Could someone that does whole mount specimens share how you count them for
workload purposes, as compared to reqular embedding and cutting?  (Prostates
are the only thing that we currently do as whole mount specimens.)

Thanks!

Bonnie Whitaker
Clinical Histology Manager
Ohio State University Medical Center
614.293.5048

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RE: [Histonet] low profile blades and cutting angle

[Whitaker, Bonnie]

I like both, but use the MX because they are cheaper, and they are, if not
quite as good as, certainly a much greater value than the AccuEdge blades (I
do think that on average I go through the MX a little faster, but they are
still cheaper!!).  I alsoo like the MB22 from ThermoFisher for cutting small,
thin sections, like renal bx.

Bonnie Whitaker

Clinical Histology Manager
Ohio State University Medical Center
N308B Doan Hall
410 W. 10th Ave.
Columbus, OH  43210

bonnie.whita...@osumc.edu

phone 614.293.5048
fax 614.293.7273
pager 614.346.5013
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum
Sent: Thursday, April 02, 2009 3:42 PM
To: Hazel V Horn
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] low profile blades and cutting angle



I am with you Hazel.  We cut paraffin and today I am cutting GMA plastic with
them.  They are great for us. 



Pam Marcum 

UPENN Vet School 

New Bolton Center 


- Original Message -
From: Hazel V Horn hor...@archildrens.org
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, April 2, 2009 3:22:09 PM GMT -05:00 US/Canada Eastern
Subject: RE: [Histonet] low profile blades and cutting angle 

We use MX premier microtome blades and order from ThermoFisher.     I do have
the accuedge blades but prefer the MX blades.   The accuedge blades just
don't seem to be as good as they used to be...my opinion only. 

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202 
  
phone   501.364.4240
fax        501.364.3155 
  
visit us on the web at:    www.archildrens.org 
  
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice
Frederick
Sent: Thursday, April 02, 2009 1:20 PM
To: 'Merced Leiker'; barricksta...@yahoo.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] low profile blades and cutting angle 

It's all I ever use and I've tried a bunch. 
Bernice 


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced Leiker
Sent: Thursday, April 02, 2009 12:17 PM
To: barricksta...@yahoo.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] low profile blades and cutting angle 

AccuEdge by Sakura Finetek are the best (avail. through VWR) 

Not only have I tried a few other brands, but our former knowledgeable and
experienced histotech here at UB recommends AccuEdge. 

Merced 

--On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick
barricksta...@yahoo.com wrote: 

 Hi everyone,
   
 What brand low profile blades does everyone prefer and where do you 
 order them?
 Also, what blade angle does everyone prefer for cryosectioning? 
   
 Thanks
 
 Stacey
 
 
 
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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences State University of New York at
Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725 

No trees were harmed in the sending of this email. 
However, many electrons were severely inconvenienced. 


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*
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The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in 

RE: [Histonet] which one first - antigen retrieval or endoperoxidase block?

[Whitaker, Bonnie]

 
Hi Everyone,

I have always done the quench 1st if I do it manually.  If it is automated,
then unless you have a newer instrument with onboard retrieval, you don't
have a choice but to quench after the retrieval step (and may not have a
choice then depending upon the system and how open it is).

Bonnie Whitaker

Clinical Histology Manager
Ohio State University Medical Center
N308B Doan Hall
410 W. 10th Ave.
Columbus, OH  43210

[EMAIL PROTECTED]

phone 614.293.5048
fax 614.293.7273
pager 614.346.5013

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Greg Dobbin
Sent: Thursday, September 25, 2008 3:12 PM
To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] which one first - antigen retrieval or endoperoxidase
block?

I'd say it doesn't matter which is 1st, except my inclination is always to do
the quench step 1st because the tissues seem a little less delicate before
retreival than after.
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

And in the end it's not the years in your life that count. It's the life in
your years. 
- Abraham Lincoln


 Monfils, Paul [EMAIL PROTECTED] 9/25/2008 3:26:01 PM 
When doing immunoperoxidase staining on a tissue that requires antigen
retrieval, which do you do first - the antigen retrieval or the endogenous
peroxidase blocking?  Or does it matter?
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[Histonet] Job in Columbus, Ohio

[Whitaker, Bonnie]

Hi Everyone,

We have a night shift opening at Ohio State University Medical Center for a
senior level histotech.  The ideal candidate will be HT(ASCP) or HTL(ASCP),
be a motivated, self-starter, and enjoy the fast-paced environment in a large
teaching hospital.

OSUMC is a great place to be!  Feel free to contact me with any questions
regarding the position!

Bonnie Whitaker

Clinical Histology Manager
Ohio State University Medical Center
N308B Doan Hall
410 W. 10th Ave.
Columbus, OH  43210

[EMAIL PROTECTED]

phone 614.293.5048
fax 614.293.7273

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