Re: [Histonet] CD68 and CD204 for dogs/pigs
Hi all, I do have MAC387 and it works great in dog and pig tissue. The PI is asking for additional CD markers to macrophages (CD68 and 204). I need them to work on dogs and pigs, but of course, there's not many vendors who list species cross-reactivities on their data sheets beyond the usual human and mouse. In my earlier trials years ago with CD68 (clone KP1), I didn't have success using it on dog tissue. Am looking for a different clone that will cross-react with dogs. Jan On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala l...@premierlab.com wrote: Jan MAC387 is a marker that we have used successfully in canine and porcine, not entirely specific to macs but may work for your situation. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 24, 2015 3:59 PM To: histonet Subject: [Histonet] CD68 and CD204 for dogs/pigs Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD68 and CD204 for dogs/pigs
Hello Jan, I will stain ED1 (cd68) for you on dog tissue Friday. I hope it works. On Wed, Feb 25, 2015 at 7:42 PM, Jan Shivers shive...@umn.edu wrote: Hi all, I do have MAC387 and it works great in dog and pig tissue. The PI is asking for additional CD markers to macrophages (CD68 and 204). I need them to work on dogs and pigs, but of course, there's not many vendors who list species cross-reactivities on their data sheets beyond the usual human and mouse. In my earlier trials years ago with CD68 (clone KP1), I didn't have success using it on dog tissue. Am looking for a different clone that will cross-react with dogs. Jan On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala l...@premierlab.com wrote: Jan MAC387 is a marker that we have used successfully in canine and porcine, not entirely specific to macs but may work for your situation. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 24, 2015 3:59 PM To: histonet Subject: [Histonet] CD68 and CD204 for dogs/pigs Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68 and CD204 for dogs/pigs
Jan MAC387 is a marker that we have used successfully in canine and porcine, not entirely specific to macs but may work for your situation. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 24, 2015 3:59 PM To: histonet Subject: [Histonet] CD68 and CD204 for dogs/pigs Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68 and CD204 for dogs/pigs
I just received Cd204 from Biorbyt but have not validated a protocol. They are located in the UK but have a distributor in San Francisco so shipping is not outrageous. References to its application: The class A macrophage scavenger receptor CD204 is a useful immunohistochemical marker of canine histiocytic sarcoma. J Comp Pathol 2013 Feb 16;148(2-3):188-96. Epub 2012 Aug 16. Y Kato, M Murakami, Y Hoshino, T Mori, K Maruo, A Hirata, T L D R Nakagawa, T Yanai, H Sakaiand Immunocytochemical detection of the class A macrophage scavenger receptor CD204 using air-dried cytologic smears of canine histiocytic sarcoma Yuki Kato1, Risa Funato1, Akihiro Hirata, Mami Murakami, Takashi Mori, Koji Maruo, Tokuma Yanai and Hiroki Sakai Article first published online: 28 AUG 2014 If you or anyone has had success with this antibody, please let me know. Thanks, Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 24, 2015 3:59 PM To: histonet Subject: [Histonet] CD68 and CD204 for dogs/pigs Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD68 and CD204 for dogs/pigs
Hi, MAC387 is a great marker but it detects less macrophages than CD68. In our experience (sheep and cattle), MAC387 is good for labelling macrophages that have recently reach the lesion, while those resident or long standing macrophages would be negative to MAC387 (but mostly positive to CD68, which is a more general marker for macs). No idea about CD204 though. Just in case this helps Regards Julio On 25/02/2015 18:08, Elizabeth Chlipala wrote: Jan MAC387 is a marker that we have used successfully in canine and porcine, not entirely specific to macs but may work for your situation. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 24, 2015 3:59 PM To: histonet Subject: [Histonet] CD68 and CD204 for dogs/pigs Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
