[Histonet] Hemoglobin stain
Hi all, I have a pathologist that is looking for a special stain that highlights blood. Initially she had wanted to use benzidine as referenced in the article below but our safety office let me know that we would need some pretty hefty air scrubbers and respirators to use it safely. Does anyone have a protocol that might give the same results? Thank you, Erin Martin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Am J Dermatopathol. 1995 Aug;17(4):362-7. Benzidine stain for the histochemical detection of hemoglobin in splinter hemorrhage (subungual hematoma) and black heel. Hafner J(1), Haenseler E, Ossent P, Burg G, Panizzon RG. Author information: (1)Department of Dermatology, University Hospital of Zurich, Switzerland. Minor nail trauma may cause bluish discoloration of the nail, while tangential skin trauma on the heel can result in a so-called black heel. To rule out melanoma in such clinical situations, a biopsy is needed to reveal homogeneous eosinophilic masses deposited under the nail plate or within it (transepidermal elimination). Most dermatopathologists attempt to demonstrate the presence of hemoglobin in these eosinophilic masses with Prussian blue stain, which typically remains negative. In our experience, these traumatically induced blood deposits are always situated in avascular spaces, devoid of degrading phagocytes. Consequently, a histochemical stain for these deposits should be directed specifically toward hemoglobin, not hemosiderin. In the dermatopathologic literature, the various techniques to detect hemoglobin deposits in tissue sections are not well-known. We would like to emphasize benzidine stain, a highly selective and efficient method to demonstrate the presence of hemoglobin deposits in histologic sections. To date, benzidine stain has not been utilized to characterize splinter hemorrhage (subungual hematoma). Of concern, the use of benzidine in histopathology laboratories is restricted because this agent is a known carcinogen, while the non-mutagenic derivative, 3,3',5,5'-tetramethylbenzidine, does not stain histologic sections. Patent blue V, a completely different and less specific agent, stains hemoglobin an intense blue-green. Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Hemoglobin A
Cell Marque does have a rabbit monoclonal antibody to Hemoglobin A, cat# 360R Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, December 02, 2010 5:52 PM To: Histonet Subject: [Histonet] Hemoglobin A Howdy histoland, does anyone know where to get Hemoglobin A? Dako doesn't have it any more and we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc, etc, etc, Biogenex. No luck. Thanks Joe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hemoglobin A
Howdy histoland, does anyone know where to get Hemoglobin A? Dako doesn't have it any more and we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc, etc, etc, Biogenex. No luck. Thanks Joe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Hemoglobin A
We just got some from BioGenex order number is AR021-5R. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, December 02, 2010 5:52 PM To: Histonet Subject: [Histonet] Hemoglobin A Howdy histoland, does anyone know where to get Hemoglobin A? Dako doesn't have it any more and we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc, etc, etc, Biogenex. No luck. Thanks Joe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hemoglobin A
thanks y'all for the info Joe - Original Message - From: Feher, Stephen sfe...@cmc-nh.org To: Joe Nocito jnoc...@satx.rr.com; Histonet histonet@lists.utsouthwestern.edu Sent: Monday, December 06, 2010 4:15 PM Subject: RE: [Histonet] Hemoglobin A We just got some from BioGenex order number is AR021-5R. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, December 02, 2010 5:52 PM To: Histonet Subject: [Histonet] Hemoglobin A Howdy histoland, does anyone know where to get Hemoglobin A? Dako doesn't have it any more and we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc, etc, etc, Biogenex. No luck. Thanks Joe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Hemoglobin Stain
Hi Linda, Thanks for the reply! No, I'm not limited to staining only. In fact, I will be performing some seperate IHC to demonstrate a couple of other proteins. It is simply my preference to use a histochemical technique for the following reasons. Firstly, from what I understand, staining will not be as laborious as IHC nor as difficult/technical to obtain positive results. Secondly, as this is part of my Honours year project, I would like to try my luck with several different techniques, and hemoglobin is the only molecule from the products I am looking at for which a simple histochemical approach is possible. Again, any suggestions would be highly valuable. Cheers, Anna Subject: RE: [Histonet] Hemoglobin Stain Date: Thu, 15 Jul 2010 11:41:54 -0500 From: lseb...@uwhealth.org To: annatay...@hotmail.com; histonet@lists.utsouthwestern.edu Anna, Are you limited to histochemical stains? Because if you're not, hemoglobin may be detected by immunohistochemistry quite nicely. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Taylor Sent: Thursday, July 15, 2010 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hemoglobin Stain Hi all, In my quest for advice about what will be my very first attempt at histology, I have been recommended to post my query here. I'm attempting to demonstrate hemoglobin in FFPE lymph node sections. I've been able to find a couple of techniques (namely the Dunn Thompson technique and Okajima's technique), although I'm not sure how suitable either would be on lymph node sections? As I said, this will be my debut experience in histology/histochemistry so any advice/recommendations at all will be appreciated :)Thanks,Anna. _ View photos of singles in your area! Looking for a hot date? http://clk.atdmt.com/NMN/go/150855801/direct/01/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ If It Exists, You'll Find it on SEEK. Australia's #1 job site http://clk.atdmt.com/NMN/go/157639755/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hemoglobin Stain
Hi all, In my quest for advice about what will be my very first attempt at histology, I have been recommended to post my query here. I'm attempting to demonstrate hemoglobin in FFPE lymph node sections. I've been able to find a couple of techniques (namely the Dunn Thompson technique and Okajima's technique), although I'm not sure how suitable either would be on lymph node sections? As I said, this will be my debut experience in histology/histochemistry so any advice/recommendations at all will be appreciated :)Thanks,Anna. _ View photos of singles in your area! Looking for a hot date? http://clk.atdmt.com/NMN/go/150855801/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Hemoglobin Stain
Anna, Are you limited to histochemical stains? Because if you're not, hemoglobin may be detected by immunohistochemistry quite nicely. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Taylor Sent: Thursday, July 15, 2010 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hemoglobin Stain Hi all, In my quest for advice about what will be my very first attempt at histology, I have been recommended to post my query here. I'm attempting to demonstrate hemoglobin in FFPE lymph node sections. I've been able to find a couple of techniques (namely the Dunn Thompson technique and Okajima's technique), although I'm not sure how suitable either would be on lymph node sections? As I said, this will be my debut experience in histology/histochemistry so any advice/recommendations at all will be appreciated :)Thanks,Anna. _ View photos of singles in your area! Looking for a hot date? http://clk.atdmt.com/NMN/go/150855801/direct/01/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
