Re: [Histonet] IHC Equipment
Sheila It is impossible to always get everything into an e-mail when you recieve a general info request. I thought what you said was great. Have a wonderful weekend. Vikki On Fri, Feb 25, 2011 at 10:25 AM, Sheila Fonner wrote: > Vikki, > > > > You are absolutely right! We do all of our ISH as overnight runs to avoid > the problem of space constraint with the detection kits, etc. I agree that > all of the instruments have their good and bad points, and it really depends > on the lab and what kind of specimens/volume you have. > > > > Didn’t mean to offend anyone. I REALLY was just trying to help and give my > opinion based on my own personal use. > > > > Sheila > > > > > > *From:* Victoria Baker [mailto:bakevicto...@gmail.com] > *Sent:* Friday, February 25, 2011 10:18 AM > *To:* Greg Dobbin > *Cc:* Sheila Fonner; histonet@lists.utsouthwestern.edu; Cynthia Pyse > *Subject:* Re: [Histonet] IHC Equipment > > > > In regards to the Ventana Ultra - if you are running PIN-4 or ISH > simultaneously on this instrument, the kits/ab's/probes they require take > up a lot of space on the reagent carousel which limits the number of > antibodies you can put on and as runs can go some where around 6-hours you > could find yourself limited in what you can run without careful planning. > > > > I'm a long time user and believer in Ventana, but BondMax and Intellipath > do have my loyalties as well. It depends on the needs of your lab > (volume/work flow, available lab space and antibody library size), the > staffing you have available and their abilities with IHC/ISH. I've done > this before and the only way to really know if an instrument will work in > your lab is to demo them and connect with other users of the instrument. > > > > Vikki > > > > > > > > > > > > > On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin wrote: > > The Bond does do ISH. In fact it utilizes the same detection kit so you > have only to buy the probes, not the additional detection kit that could > (depending on your volume of ISH requests) expire. > Greg > > Greg Dobbin, R.T. > Chief Technologist, Anatomic Pathology > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PEC1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > "I find that the harder I work, the > more luck I seem to have." > - Thomas Jefferson > > > >>> "Sheila Fonner" 2/25/2011 10:20 AM >>> > > Cindy, > > We used to use the Dako stainer, and we still have it as a back-up if > necessary, but we have recently (in the last year) bought a Ventana Ultra. > You can put 30 slides at a time on it, but you do not have to batch the > slides. It is a continuous feed machine, which means that as soon as a > slide is done, you can take it off and run something else. You do not have > to have all of the slides from a case together side by side. It is > bar-code > driven and will find the slides no matter where you put them. Each slide > drawer runs independently of the others. Ours has been wonderful. The > technical assistance is fantastic also. They will help you to initially > work up all of your antibodies. You can use theirs, or you can use third > party antibodies and place them in a prep-kit. You can also use RTU or > concentrates. It's really up to you. I would highly recommend it if you > have a large volume. We demo'd the Biocare Intellipath also. I liked the > machine, but it was really just a step up from the Dako. Leica has the > Bond > instruments, which a lot of people like, but for us, it didn't work because > we wanted to be able to do ISH. Also, the Leica limits you to drawers of > ten slides. So when you load a drawer, with 1 slide or 10, you can't use > it > again until that run is finished. Hope this helps. If you have any > questions, you can contact me. > Have a great day! > > Sheila > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia > Pyse > Sent: Wednesday, February 23, 2011 2:37 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC Equipment > > Hi Histonetters > > I currently use a Dako stainer for my IHC staining. It is a work horse with > very little problems. It is a older model that we may need to replace in > the > near future. What is everyone using out in histoland. I would be perfectly > willing to purchase another Dako but I want to explore all avenues before > making a decision. What are the pros and cons of the inst
RE: [Histonet] IHC Equipment
Vikki, You are absolutely right! We do all of our ISH as overnight runs to avoid the problem of space constraint with the detection kits, etc. I agree that all of the instruments have their good and bad points, and it really depends on the lab and what kind of specimens/volume you have. Didn't mean to offend anyone. I REALLY was just trying to help and give my opinion based on my own personal use. Sheila From: Victoria Baker [mailto:bakevicto...@gmail.com] Sent: Friday, February 25, 2011 10:18 AM To: Greg Dobbin Cc: Sheila Fonner; histonet@lists.utsouthwestern.edu; Cynthia Pyse Subject: Re: [Histonet] IHC Equipment In regards to the Ventana Ultra - if you are running PIN-4 or ISH simultaneously on this instrument, the kits/ab's/probes they require take up a lot of space on the reagent carousel which limits the number of antibodies you can put on and as runs can go some where around 6-hours you could find yourself limited in what you can run without careful planning. I'm a long time user and believer in Ventana, but BondMax and Intellipath do have my loyalties as well. It depends on the needs of your lab (volume/work flow, available lab space and antibody library size), the staffing you have available and their abilities with IHC/ISH. I've done this before and the only way to really know if an instrument will work in your lab is to demo them and connect with other users of the instrument. Vikki On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin wrote: The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Sheila Fonner" 2/25/2011 10:20 AM >>> Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Co
RE: [Histonet] IHC Equipment
Sorry if I confused anyone. It may have been FISH that we couldn't do on the Leica instrument. There was some reason that the docs chose to go with the Ventana. Anyway, I'm sure they are both fine instruments! Sheila From: Greg Dobbin [mailto:gvdob...@ihis.org] Sent: Friday, February 25, 2011 9:31 AM To: Sheila Fonner; histonet@lists.utsouthwestern.edu; 'Cynthia Pyse' Subject: RE: [Histonet] IHC Equipment The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Sheila Fonner" 2/25/2011 10:20 AM >>> Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC Equipment
In regards to the Ventana Ultra - if you are running PIN-4 or ISH simultaneously on this instrument, the kits/ab's/probes they require take up a lot of space on the reagent carousel which limits the number of antibodies you can put on and as runs can go some where around 6-hours you could find yourself limited in what you can run without careful planning. I'm a long time user and believer in Ventana, but BondMax and Intellipath do have my loyalties as well. It depends on the needs of your lab (volume/work flow, available lab space and antibody library size), the staffing you have available and their abilities with IHC/ISH. I've done this before and the only way to really know if an instrument will work in your lab is to demo them and connect with other users of the instrument. Vikki On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin wrote: > The Bond does do ISH. In fact it utilizes the same detection kit so you > have only to buy the probes, not the additional detection kit that could > (depending on your volume of ISH requests) expire. > Greg > > Greg Dobbin, R.T. > Chief Technologist, Anatomic Pathology > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PEC1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > "I find that the harder I work, the > more luck I seem to have." > - Thomas Jefferson > > > >>> "Sheila Fonner" 2/25/2011 10:20 AM >>> > Cindy, > > We used to use the Dako stainer, and we still have it as a back-up if > necessary, but we have recently (in the last year) bought a Ventana Ultra. > You can put 30 slides at a time on it, but you do not have to batch the > slides. It is a continuous feed machine, which means that as soon as a > slide is done, you can take it off and run something else. You do not have > to have all of the slides from a case together side by side. It is > bar-code > driven and will find the slides no matter where you put them. Each slide > drawer runs independently of the others. Ours has been wonderful. The > technical assistance is fantastic also. They will help you to initially > work up all of your antibodies. You can use theirs, or you can use third > party antibodies and place them in a prep-kit. You can also use RTU or > concentrates. It's really up to you. I would highly recommend it if you > have a large volume. We demo'd the Biocare Intellipath also. I liked the > machine, but it was really just a step up from the Dako. Leica has the > Bond > instruments, which a lot of people like, but for us, it didn't work because > we wanted to be able to do ISH. Also, the Leica limits you to drawers of > ten slides. So when you load a drawer, with 1 slide or 10, you can't use > it > again until that run is finished. Hope this helps. If you have any > questions, you can contact me. > Have a great day! > > Sheila > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia > Pyse > Sent: Wednesday, February 23, 2011 2:37 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC Equipment > > Hi Histonetters > > I currently use a Dako stainer for my IHC staining. It is a work horse with > very little problems. It is a older model that we may need to replace in > the > near future. What is everyone using out in histoland. I would be perfectly > willing to purchase another Dako but I want to explore all avenues before > making a decision. What are the pros and cons of the instruments any of you > are using. How often is the machine down? What is the capacity? We run the > Dako twice daily usually to the capacity of 48 slides. I would like to hear > only from actual user of the instrumentation, no vendors please. This is > only a fact finding e-mail. Thanks in advance for all your input. > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory/Histology Supervisor > > X-Cell Laboratories > > 716-250-9235 > > e-mail cp...@x-celllab.com > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > - > Statement of Confidentiality > This message (including attachments) may contain confidential or privileged > information intended for a specific individual or organization. If you have > received this c
RE: [Histonet] IHC Equipment
The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Sheila Fonner" 2/25/2011 10:20 AM >>> Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC Equipment
Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a slide is done, you can take it off and run something else. You do not have to have all of the slides from a case together side by side. It is bar-code driven and will find the slides no matter where you put them. Each slide drawer runs independently of the others. Ours has been wonderful. The technical assistance is fantastic also. They will help you to initially work up all of your antibodies. You can use theirs, or you can use third party antibodies and place them in a prep-kit. You can also use RTU or concentrates. It's really up to you. I would highly recommend it if you have a large volume. We demo'd the Biocare Intellipath also. I liked the machine, but it was really just a step up from the Dako. Leica has the Bond instruments, which a lot of people like, but for us, it didn't work because we wanted to be able to do ISH. Also, the Leica limits you to drawers of ten slides. So when you load a drawer, with 1 slide or 10, you can't use it again until that run is finished. Hope this helps. If you have any questions, you can contact me. Have a great day! Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC Equipment
Cindy We have three dako autostainers and we really like them a lot. We are a contract research lab so flexibility and system openness is key for us. The only other instrument that I have demoed is the Leica Bond, but unless I purchased the research software (which is a bit expensive) I was tied into using at least one of their reagents and their reagents are purchased in kits, so for my lab it was not a good fit financially since I already had two dako stainers on reagent rental. If you have the space I would demo the different instruments on the market, see which one works best for you. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 23, 2011 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Equipment Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Equipment
Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC equipment
This is a matter of opinion.? DO you like a closed system or an open system?? My preference is an open system and I prefer Biocare.? As for the PIN-4 Cocktail, Biocare wins hands down there as well...as Dr Tacha works there. -Original Message- From: richard wells <[EMAIL PROTECTED]> To: histonet Sent: Thu, 30 Oct 2008 2:17 pm Subject: [Histonet] IHC equipment I am helping to set up an in office lab for a group of urologists.? What is the best instrumentation for prostate tripple stain?? What are the best reagents? ? Test volume will be relatively low.? Perhaps as many as 5 additional immunostains will be added in the future.? Testing will be done by an experienced histotech with minimal exposure to immunoperoxidase techniques. ? Goals are to maximize simplicity, dependability and value. ? Thanks in advance for any and all advice. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC equipment
I am helping to set up an in office lab for a group of urologists. What is the best instrumentation for prostate tripple stain? What are the best reagents? Test volume will be relatively low. Perhaps as many as 5 additional immunostains will be added in the future. Testing will be done by an experienced histotech with minimal exposure to immunoperoxidase techniques. Goals are to maximize simplicity, dependability and value. Thanks in advance for any and all advice. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet