Re: [Histonet] IHC gunk on slides
Karen,
Regardless of the instrument you’re using, if related to a reagent, it may
be that your detection chromogen is breaking down and you need to report
it, or you have a material that is prepared onsite, and used onboard the
instrument, and unknowingly contaminated.
it may help if you can provide an image. It can be done with a mobile phone
up to the eyepiece if necessary.
Best wishes,
Eddie Martin
The National Institutes of Health
301-594-2054
On Thu, Mar 23, 2023 at 1:00 PM
wrote:
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>1. IHC gunk (Karen Heckford CA-San Francisco)
>2. Re: IHC gunk (Willis, Donna G)
>3. Re: IHC gunk (Thomas Podawiltz)
>4. Re: IHC gunk (Mac Donald, Jennifer)
>5. Re: Histonet Digest, Vol 232, Issue 7 (Eddie Martin)
>
>
>
> -- Forwarded message --
> From: Karen Heckford CA-San Francisco
> To: Histonet
> Cc:
> Bcc:
> Date: Wed, 22 Mar 2023 10:45:25 -0700
> Subject: [Histonet] IHC gunk
> Good Morning,
> I have recently developed a problem with contamination of some kind on my
> IHC slides.
> The contamination is black clumps and lays on top of the tissue. I have
> been told it is bacteria but the Pathologist and I kind of doubt that. I
> cannot get a good picture of it to show. It may not be on all the slides
> on the same run or even the same antibody slide.
>
> I have decontaminated the whole system. I have put fresh reagents on the
> instrument. This stuff looks like it would wash off at the end of the run
> since it is sitting right on top of the tissue. I do not see this stuff
> elsewhere on the slide, only the tissue. Does not matter if the tissue
> was cut fresh or not.
>
> I have tried everything I can think of to get rid of it and still have this
> issue. I use DiH20 from the hospital system. Not sure if this may be the
> problem. I can have Engineering test the DiH20.
>
> Any help would be greatly appreciated.
>
> Thanks,
>
> Karen Heckford HT ASCP CE
>
> Lead Histology Technician
>
> St. Mary's Medical Center
>
> 450 Stanyan St.
>
> San Francisco, Ca. 94117
>
> 415-750-5751
>
> karen.heckford@ commonspirit.org
>
>
>
>
> Caution: This email message, including all content and attachments, is
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> information contained in this email message is intended only for the use of
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> intended recipient or an agent responsible for delivering it to the
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>
> -- Forwarded message --
> From: "Willis, Donna G"
> To: Karen Heckford CA-San Francisco ,
> Histonet
> Cc:
> Bcc:
> Date: Wed, 22 Mar 2023 17:57:09 +
> Subject: Re: [Histonet] IHC gunk
> What instrumentation are you using. Have you talked to your vendor?
>
> Donna Willis
> Anatomic Pathology Manager
> Baylor Scott Health
> Baylor University Medical Center
> 3500 Gaston Ave|Dallas, Texas 75246
> 214-820-2465 office|214-725-6184 mobile
>
>
>
> -Original Message-
> From: Karen Heckford CA-San Francisco via Histonet <
> histonet@lists.utsouthwestern.edu>
> Sent: Wednesday, March 22, 2023 12:45 PM
> To: Histonet
> Subject: {EXTERNAL} [Histonet] IHC gunk
>
>
> CAUTION: This email originated outside of BSWH. The actual sender is (
> histonet-boun...@lists.utsouthwestern.edu) which may be different from
> the display address in the From: field. Avoid action unless you know
Re: [Histonet] IHC gunk
Is it possible that it is hematoxylin precipitate? -Original Message- From: Karen Heckford CA-San Francisco via Histonet Sent: Wednesday, March 22, 2023 10:45 AM To: Histonet Subject: [Histonet] IHC gunk EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Good Morning, I have recently developed a problem with contamination of some kind on my IHC slides. The contamination is black clumps and lays on top of the tissue. I have been told it is bacteria but the Pathologist and I kind of doubt that. I cannot get a good picture of it to show. It may not be on all the slides on the same run or even the same antibody slide. I have decontaminated the whole system. I have put fresh reagents on the instrument. This stuff looks like it would wash off at the end of the run since it is sitting right on top of the tissue. I do not see this stuff elsewhere on the slide, only the tissue. Does not matter if the tissue was cut fresh or not. I have tried everything I can think of to get rid of it and still have this issue. I use DiH20 from the hospital system. Not sure if this may be the problem. I can have Engineering test the DiH20. Any help would be greatly appreciated. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.heckford@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet=05%7C01%7Cjmacdonald%40mtsac.edu%7C27946f7c47f74e58436a08db2afd6592%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C638151040067352929%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C=coq7k8kIhG03oke%2B477suyaDCCby1zEujMseWZ4lQSw%3D=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC gunk
Is it on all slides or just certain antibodies?
Sent from Yahoo Mail for iPhone
On Wednesday, March 22, 2023, 1:57 PM, Willis, Donna G via Histonet
wrote:
What instrumentation are you using. Have you talked to your vendor?
Donna Willis
Anatomic Pathology Manager
Baylor Scott Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile
-Original Message-
From: Karen Heckford CA-San Francisco via Histonet
Sent: Wednesday, March 22, 2023 12:45 PM
To: Histonet
Subject: {EXTERNAL} [Histonet] IHC gunk
CAUTION: This email originated outside of BSWH. The actual sender is
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Outlook ribbon.
Good Morning,
I have recently developed a problem with contamination of some kind on my IHC
slides.
The contamination is black clumps and lays on top of the tissue. I have
been told it is bacteria but the Pathologist and I kind of doubt that. I
cannot get a good picture of it to show. It may not be on all the slides on
the same run or even the same antibody slide.
I have decontaminated the whole system. I have put fresh reagents on the
instrument. This stuff looks like it would wash off at the end of the run
since it is sitting right on top of the tissue. I do not see this stuff
elsewhere on the slide, only the tissue. Does not matter if the tissue
was cut fresh or not.
I have tried everything I can think of to get rid of it and still have this
issue. I use DiH20 from the hospital system. Not sure if this may be the
problem. I can have Engineering test the DiH20.
Any help would be greatly appreciated.
Thanks,
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-750-5751
karen.heckford@ commonspirit.org
Caution: This email message, including all content and attachments, is
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information contained in this email message is intended only for the use of the
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recipient or an agent responsible for delivering it to the intended recipient,
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distribution, or copying of this message is strictly prohibited. If you have
received this communication in error, please notify us immediately by reply
email. Thank you
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transmission to (or receipt by) any unauthorized person(s). If you believe that
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Then destroy it and any attachments. Thank you.
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Re: [Histonet] IHC gunk
What instrumentation are you using. Have you talked to your vendor?
Donna Willis
Anatomic Pathology Manager
Baylor Scott Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile
-Original Message-
From: Karen Heckford CA-San Francisco via Histonet
Sent: Wednesday, March 22, 2023 12:45 PM
To: Histonet
Subject: {EXTERNAL} [Histonet] IHC gunk
CAUTION: This email originated outside of BSWH. The actual sender is
(histonet-boun...@lists.utsouthwestern.edu) which may be different from the
display address in the From: field. Avoid action unless you know the content is
safe. Report suspicious emails using the PhishAlarm button located in your
Outlook ribbon.
Good Morning,
I have recently developed a problem with contamination of some kind on my IHC
slides.
The contamination is black clumps and lays on top of the tissue. I have
been told it is bacteria but the Pathologist and I kind of doubt that. I
cannot get a good picture of it to show. It may not be on all the slides on
the same run or even the same antibody slide.
I have decontaminated the whole system. I have put fresh reagents on the
instrument. This stuff looks like it would wash off at the end of the run
since it is sitting right on top of the tissue. I do not see this stuff
elsewhere on the slide, only the tissue. Does not matter if the tissue
was cut fresh or not.
I have tried everything I can think of to get rid of it and still have this
issue. I use DiH20 from the hospital system. Not sure if this may be the
problem. I can have Engineering test the DiH20.
Any help would be greatly appreciated.
Thanks,
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-750-5751
karen.heckford@ commonspirit.org
Caution: This email message, including all content and attachments, is
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The
information contained in this email message is intended only for the use of the
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recipient or an agent responsible for delivering it to the intended recipient,
you have received this document in error. Any further review, dissemination,
distribution, or copying of this message is strictly prohibited. If you have
received this communication in error, please notify us immediately by reply
email. Thank you
Caution: This email is both proprietary and confidential, and not intended for
transmission to (or receipt by) any unauthorized person(s). If you believe that
you have received this email in error, do not read any attachments. Instead,
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Then destroy it and any attachments. Thank you.
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[Histonet] IHC gunk
Good Morning, I have recently developed a problem with contamination of some kind on my IHC slides. The contamination is black clumps and lays on top of the tissue. I have been told it is bacteria but the Pathologist and I kind of doubt that. I cannot get a good picture of it to show. It may not be on all the slides on the same run or even the same antibody slide. I have decontaminated the whole system. I have put fresh reagents on the instrument. This stuff looks like it would wash off at the end of the run since it is sitting right on top of the tissue. I do not see this stuff elsewhere on the slide, only the tissue. Does not matter if the tissue was cut fresh or not. I have tried everything I can think of to get rid of it and still have this issue. I use DiH20 from the hospital system. Not sure if this may be the problem. I can have Engineering test the DiH20. Any help would be greatly appreciated. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.heckford@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
