Re: [Histonet] microtome blades and wax
Jessica, What has Leica said about the problem? I have always found them to be responsive and able to provide suggestions. This would be especially true with a new instrument, still under warranty. We have never had any inconsistencies with our Autocut, which was purchased last spring. Toni NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -Original Message- From: Piche, Jessica via Histonet Sent: Friday, January 14, 2022 8:43 AM To: Histonet Subject: [Histonet] microtome blades and wax *** This is an External Email *** Good Morning, We have been using Leica Histocore Autocut microtomes for a few months and have been having inconsistency with cutting on them. We have tried so many different blades, high and low, and have tried changing the blade angle. Nothing is a perfect fix, and we feel like we are really having to work much harder to get a great section. We were wondering what everyone else uses. I was also wondering if the wax could be an issue. We use EM-400 for embedding. I'd appreciate anyone's thoughts. Thank you and have a nice weekend! Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!uiyCL_cBeR_FRDl6tMCr34gjeUYcHEGHSOx1bIEuIcwqytIu5aQZnnf0pnjQJj2bw_sa$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microtome blades and wax
Hi Jessica, Happy Friday. Do you use the same EM-400 paraffin for processing? If you use different paraffins that might contribute to your sectioning problems. If the waxes are different the tissue and the rest of the block are not one continuous unit in the "eyes" of the knife. The waxes have different properties so they cut differently because the embedding wax does not infiltrate into the tissue but wraps around it like a blanket. I'm not sure if that's what your lab does but if it is I suggest using the same paraffin for processing and embedding. Sincerely, Paula Sicurello Sent from my iPhone > On Jan 14, 2022, at 5:43 AM, Piche, Jessica via Histonet > wrote: > > Good Morning, > > We have been using Leica Histocore Autocut microtomes for a few months and > have been having inconsistency with cutting on them. We have tried so many > different blades, high and low, and have tried changing the blade angle. > Nothing is a perfect fix, and we feel like we are really having to work much > harder to get a great section. We were wondering what everyone else uses. I > was also wondering if the wax could be an issue. We use EM-400 for embedding. > I'd appreciate anyone's thoughts. > > Thank you and have a nice weekend! > > Jessica > > Jessica Piche, HT(ASCP) > Waterbury Hospital Histology Laboratory > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microtome blades and wax
Good Morning, We have been using Leica Histocore Autocut microtomes for a few months and have been having inconsistency with cutting on them. We have tried so many different blades, high and low, and have tried changing the blade angle. Nothing is a perfect fix, and we feel like we are really having to work much harder to get a great section. We were wondering what everyone else uses. I was also wondering if the wax could be an issue. We use EM-400 for embedding. I'd appreciate anyone's thoughts. Thank you and have a nice weekend! Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome blades
Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome blades
Sakura René J. From: Laurie Colbert lcolb...@pathmdlabs.com To: Histonet Post (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 10:55 AM Subject: [Histonet] Microtome blades Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome blades
If you order them from Cardinal they get them from SakuraI think. Or maybe vice-versa... Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 09, 2013 12:15 PM To: Laurie Colbert; Histonet Post (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Microtome blades Sakura René J. From: Laurie Colbert lcolb...@pathmdlabs.com To: Histonet Post (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 10:55 AM Subject: [Histonet] Microtome blades Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome Blades
The best blades we've used so far are from Mercedes Medical - MER DMBHPGP - and are priced well below some competitors blades. Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
