Re: [Histonet] microwave processing
From the vendor perspective, we have definitely seen an increase in implementation of our processors. Going to the xylene-free, isopropanol clearing approach has lead to converts because the process is more gentle (xylene can harden dense tissues and over clear small specimens). Before coming to work on the vendor side, I have been using this technology for over 20 years :) From: ewj--- via Histonet Sent: Wednesday, February 14, 2024 9:36:52 PM To: Gudrun Lang ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] microwave processing We have a sakura VIP5 but we have been processing most of our histopath with DIY-modified microwave and no xylene for several years now. We still use the old VIP5 in xylene mode for large batches of stuff just to save labor time. I have no idea what others do. E. Wayne Johnson DVM Enable AgTech Beijing -Original Message- From: Gudrun Lang via Histonet Reply-To: Gudrun Lang To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processing Date: Tue 10:41 PM Dear histonetters! I have a question for those who have an insight in the whole landscape of pathologies. I am reading the microwave application book of Dr. Leong (2009). He writes very enthusiastic about fixation and processing with mircrowaves. I know there are microwave processors for continous workflow on the market. Now I am curious, how many pathologies use this technology. What do you think? A few percent or rising numbers? Thanks in advance and kind regards Gudrun Gudrun Lang Landgutstra�e 25 4040 Linz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microwave processing
We have a sakura VIP5 but we have been processing most of our histopath with DIY-modified microwave and no xylene for several years now. We still use the old VIP5 in xylene mode for large batches of stuff just to save labor time. I have no idea what others do. E. Wayne Johnson DVM Enable AgTech Beijing -Original Message- From: Gudrun Lang via Histonet Reply-To: Gudrun Lang To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processing Date: Tue 10:41 PM Dear histonetters! I have a question for those who have an insight in the whole landscape of pathologies. I am reading the microwave application book of Dr. Leong (2009). He writes very enthusiastic about fixation and processing with mircrowaves. I know there are microwave processors for continous workflow on the market. Now I am curious, how many pathologies use this technology. What do you think? A few percent or rising numbers? Thanks in advance and kind regards Gudrun Gudrun Lang Landgutstra�e 25 4040 Linz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microwave processing
Dear histonetters! I have a question for those who have an insight in the whole landscape of pathologies. I am reading the microwave application book of Dr. Leong (2009). He writes very enthusiastic about fixation and processing with mircrowaves. I know there are microwave processors for continous workflow on the market. Now I am curious, how many pathologies use this technology. What do you think? A few percent or rising numbers? Thanks in advance and kind regards Gudrun Gudrun Lang Landgutstraße 25 4040 Linz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave Processing
Hello all, I use microwave processing exclusively. First of all, are you making sure that each reagent is completely extracted before the next reagent is administered? I know the tissue cannot be completely dry, but all residue reagent should be siphoned off before the next one goes in. This is especially important when dehydrating the tissue with ethanol, acetone, or polypropylene reagents. I usually do at least 3 resin changes if not 4. Curing the resin is another issue I've seen. Even though protocol says to cure for three days, I have found curing for a week is better. Especially important to cure the resin blocks longer in humid, rainy, or cold environments. Another issue is the diamond knives. Make sure that your clearance angle is correct and the water in the boat meets the knife edge, if you are doing TEM. Check those things and happy sectioning. Lita Sent from my Verizon, Samsung Galaxy smartphone Original message From: "Campbell, Tasha via Histonet" Date: 11/8/23 1:44 PM (GMT-06:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Processing Is there anyone that is super familiar with microwave processing? I really need some help. I am having trouble with my tissue and I think it's the processing. I don't know what else it would be. We moved to a new building so I don't know if the power could be stronger and its changing the way it processes or what. I have tried messing with the program times and temps but I cannot figure it out. My blocks keep having what looks like knife lines but it's not the microtomes. The only change has been moving into a brand new building. Any help would be appreciated! Thanks. Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 301-695-6800 ext. 144 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=ZQs-KZ8oxEw0p81sqgiaRA=Z-Quiwr_oQfGYLNUELwQ1A=V2zfrROQEcGKlRUW4kujQYF33S2YA8Y2zc14D8heDFEQhQs5URz_aaD3SVRB-BEY=Abfh6qOZ3XnmvUsraeSWPUfFcn1Ow8IUH6TreF8Ivnk= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave Processing
They may make a combo UPS/line conditioner. That would be ideal. Line conditioners stabilize and protect against power spikes/surges. Thanks, Brian -Original Message- From: Campbell, Tasha Sent: Wednesday, November 8, 2023 12:40 PM To: Cooper, Brian Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microwave Processing (EXTERNAL EMAIL) CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: tcampb...@fgamd.com. What does a line conditioner do? The company was bought out recently and basically has no employees so I have no one to help me. Tasha Campbell Sent from my iPhone > On Nov 8, 2023, at 3:36 PM, Cooper, Brian wrote: > > Hi Tasha, > > Maybe look into getting a line conditioner for your tissue processors. Your > tissue processor vendor may have some suggestions. > > Thanks, > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of > Pathology and Laboratory Medicine Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 > bcoo...@chla.usc.edu > > -Original Message- > From: Campbell, Tasha via Histonet > Sent: Wednesday, November 8, 2023 11:44 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL) > > CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside > CHLA. Do not open attachments, click on links, or respond unless you expected > this message and recognize the email address: > histonet-boun...@lists.utsouthwestern.edu. > > Is there anyone that is super familiar with microwave processing? I really > need some help. I am having trouble with my tissue and I think it's the > processing. I don't know what else it would be. We moved to a new building > so I don't know if the power could be stronger and its changing the way it > processes or what. I have tried messing with the program times and temps but > I cannot figure it out. My blocks keep having what looks like knife lines > but it's not the microtomes. The only change has been moving into a brand new > building. Any help would be appreciated! Thanks. > > > Tasha Campbell, B.S., HTL (ASCP) > Frederick Gastroenterology Associates > 7109 Guilford Dr. Suite 300 > Frederick, MD 21704 > 301-695-6800 ext. 144 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://secure-web.cisco.com/1xOy41N6xvg0UteDOIpjvHf_CApfMcmUGNNj458SM > -CGccqMtGH-FueUgDT2-PuUzi4tvd_GkWrTORVTZWS5BZJgE72NR2xJJTk01-cOaT-nzZM > tUbO3ZfdoGwdKFXuKi0Qq7wY6p0enhF_HVv0kxEW-8mnROFvKHXVmVK3ntIMO6OhYNRZVw > X-VTPtEydHHpARUr1BSXsHA3MtHNTBt4HykE0OXOK8asWk8RQW_yKcYjWxSEEwH_vD1ocX > Up48AzfKg8xNdL56OW30ndSRD5hmp0Gpp-mLx0fN5_ET34tkBsdiHLyQg0kk3pJOLACTze > dmSiOPdFtx_ZRJ-ytfdE1V08WztA5BHIJhSrt44_FhbVF5KKndReERS2HveVBcsPKFebUL > FXvEP72PvfysukCulHG3OpPnMFY_IXE2fkNA8_EQhQGsOmCVyg7Y9AICeRTtXwI9DytSpM > f7XCTujLDQ/https%3A%2F%2Furl.emailprotection.link%2F%3FbXyKyZCpvAJAuWZ > pEARN9oWjGEc0NHCYoC4VTztcJcf6GYOHMwDA04MoeflfYb1xPjKWMnZkaOCqeOtNoZ4Sw > kd-VyGHnZDhhsZd6fJhf9axt9VVVZ50GIwj4DnibSEV8tf2M7CLjuHYF9MkMPooCQ4Mt0P > ATuuXc0T64axJSX2gX7INjxTgJxGB_LdNp_wf2pRolRjTD6LT6OcXK7vrZgrIgSwnTljdL > T_anURFohI0k-BfXgqgcHF6KIYIK23zWTmIg3tG0Sbiybk0fQ_cSufokcCBEdzJ7X8qfla > g8Qfau0FsEpgdw5sDb49u1tC5rIVFbE7XPwklXgZA1K2hCImmIcuRZyO_TcmopBwK598tM > 1u4L5MHzmYR6AXF4ClgS7r2ofMK0RsiZIcnPq2daWo7Pfh6fs7eV1avQ-x0wojgsvi_XnL > is5CwLvu6oaRjdbCzHjtxYS2nfdzJYUEqHClxcA7Twh0DlyJ3vEf8iqCEdWX5p0nW9EvH9 > 11zINBjt0lUDflMBMXT4tkDigoynpB7nJH3RZDt9yBFTZ4HQSoYKUJcatXnLiZ2qw-tIY1 > GRcxdriVyv_XEubDtu2IsBa-S69JOaJXnnuwrK2EcEgHbXlTs-ZunA8_KfVoyBf6bj3K54 > 7D0LehZYi4ZTn3_l8jMqlPyw8-4eJ1SiU_iyIh4%7E > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential or > legally privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original > message. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave Processing
What does a line conditioner do? The company was bought out recently and basically has no employees so I have no one to help me. Tasha Campbell Sent from my iPhone > On Nov 8, 2023, at 3:36 PM, Cooper, Brian wrote: > > Hi Tasha, > > Maybe look into getting a line conditioner for your tissue processors. Your > tissue processor vendor may have some suggestions. > > Thanks, > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 > bcoo...@chla.usc.edu > > -Original Message- > From: Campbell, Tasha via Histonet > Sent: Wednesday, November 8, 2023 11:44 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL) > > CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside > CHLA. Do not open attachments, click on links, or respond unless you expected > this message and recognize the email address: > histonet-boun...@lists.utsouthwestern.edu. > > Is there anyone that is super familiar with microwave processing? I really > need some help. I am having trouble with my tissue and I think it's the > processing. I don't know what else it would be. We moved to a new building > so I don't know if the power could be stronger and its changing the way it > processes or what. I have tried messing with the program times and temps but > I cannot figure it out. My blocks keep having what looks like knife lines > but it's not the microtomes. The only change has been moving into a brand new > building. Any help would be appreciated! Thanks. > > > Tasha Campbell, B.S., HTL (ASCP) > Frederick Gastroenterology Associates > 7109 Guilford Dr. Suite 300 > Frederick, MD 21704 > 301-695-6800 ext. 144 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://url.emailprotection.link/?bXyKyZCpvAJAuWZpEARN9oWjGEc0NHCYoC4VTztcJcf6GYOHMwDA04MoeflfYb1xPjKWMnZkaOCqeOtNoZ4Swkd-VyGHnZDhhsZd6fJhf9axt9VVVZ50GIwj4DnibSEV8tf2M7CLjuHYF9MkMPooCQ4Mt0PATuuXc0T64axJSX2gX7INjxTgJxGB_LdNp_wf2pRolRjTD6LT6OcXK7vrZgrIgSwnTljdLT_anURFohI0k-BfXgqgcHF6KIYIK23zWTmIg3tG0Sbiybk0fQ_cSufokcCBEdzJ7X8qflag8Qfau0FsEpgdw5sDb49u1tC5rIVFbE7XPwklXgZA1K2hCImmIcuRZyO_TcmopBwK598tM1u4L5MHzmYR6AXF4ClgS7r2ofMK0RsiZIcnPq2daWo7Pfh6fs7eV1avQ-x0wojgsvi_XnLis5CwLvu6oaRjdbCzHjtxYS2nfdzJYUEqHClxcA7Twh0DlyJ3vEf8iqCEdWX5p0nW9EvH911zINBjt0lUDflMBMXT4tkDigoynpB7nJH3RZDt9yBFTZ4HQSoYKUJcatXnLiZ2qw-tIY1GRcxdriVyv_XEubDtu2IsBa-S69JOaJXnnuwrK2EcEgHbXlTs-ZunA8_KfVoyBf6bj3K547D0LehZYi4ZTn3_l8jMqlPyw8-4eJ1SiU_iyIh4~ > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential or > legally privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original > message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave Processing
Hi Tasha, Maybe look into getting a line conditioner for your tissue processors. Your tissue processor vendor may have some suggestions. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edu -Original Message- From: Campbell, Tasha via Histonet Sent: Wednesday, November 8, 2023 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL) CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-boun...@lists.utsouthwestern.edu. Is there anyone that is super familiar with microwave processing? I really need some help. I am having trouble with my tissue and I think it's the processing. I don't know what else it would be. We moved to a new building so I don't know if the power could be stronger and its changing the way it processes or what. I have tried messing with the program times and temps but I cannot figure it out. My blocks keep having what looks like knife lines but it's not the microtomes. The only change has been moving into a brand new building. Any help would be appreciated! Thanks. Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 301-695-6800 ext. 144 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://secure-web.cisco.com/1iXiR7DgxMp8b_BUwsOCVLYh5BrZ18oRqXPXSrsq6yyJTaeSklH-M9e_nxo0H1edrZXY8yW2gB8uBPQFlrkycg81-6fiZfp9m4ew3W1GIWPzMJxjQlg5CqwTVlRq0Cyjnm_YOVGfYZOiimFv4mFXsvQ4s6Z5mi6VGMQ9l9EV181iFfWy_EdrhCytI8lcrPR5T3NjeUxeI0fA9cFJZ7LWxqU--onyetKWST0IMK268d0AjEQ7OSLKvdYXVmYyVJAzxfipn9ViDmsCqXJRxf4uITFghZAI2yDQKiuWScym6vAcxOar9XYPwUrdH57HihsGPipJmQxk7mxyMmOsxjl-Hao_xLjG2Vk5YaQwnVbDu_3Wghi1tH8eSyr9-aN3yczudiSqKuNpUfLXBO5tnIwNkVhpnspiPX0-LrFtTxl5TTZGGeAUdxUxy3O4osa6kJqifCnXi6k8ZX46q32cw2YH4vw/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave Processing
Is there anyone that is super familiar with microwave processing? I really need some help. I am having trouble with my tissue and I think it's the processing. I don't know what else it would be. We moved to a new building so I don't know if the power could be stronger and its changing the way it processes or what. I have tried messing with the program times and temps but I cannot figure it out. My blocks keep having what looks like knife lines but it's not the microtomes. The only change has been moving into a brand new building. Any help would be appreciated! Thanks. Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 301-695-6800 ext. 144 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave processing special stains
Does anyone use the KOS microwave processor for their special stains? If so did you purchase the histo-module from them or have you validated another reagent jar? Trying to keep costs low at the moment so management wants to know if there is any alternative to their modules. Thanks -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave processing
Since I have been microwave processing GI biopsies, I have noticed that there is a blurry, fuzzy artifact on most of my slides in random spots on the tissue when looking under the scope. I asked the pathologist and he said he notices it too but he doesn't know what it is and it doesn't interfere with interpretation but it still bothers me. Does anyone else have this problem? I am almost certain it is from processing. I have stained some slides of tissue that were processed on a normal processor and I do not see that phenomenon. Is this due to microwave processing and is there a fix for it? Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] microwave processing
Thank you for the kind responses. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Montag, 13. Jänner 2014 19:56 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microwave processing
Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microwave processing
Perhaps you can get some literature from one of the vendors that sell that technology. Milestone Medical Sakura Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang [gu.l...@gmx.at] Sent: Monday, January 13, 2014 1:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microwave processing
Gudrun, A good overview is here: http://www.ebsciences.com/papers/mw_tech.htm A couple old books: The Microwave Cookbook for Microscopists, Boon and Kok Microwave Applications in Pathology [Hardcover] Anthony S. -Y Leong This person also wrote a lot of articles back in the 1980's and '90s on the subject Also a book by Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Monday, January 13, 2014 10:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microwave processing
Hi Gudrun: I recommend you to get The Microwave tool book by Login and Dvorak (1994) I am also sending you under separate cover an article I wrote on the subject. As to your questions, the practice of histology has concluded that: 1- the physical principle is that microwaves excite (shake) all chemical molecules with electrical charge and, in consequence, that shaking produces heat. That is why paraffin and any non-polar molecule cannot be heated in a MW oven per se. 2- infiltration is faster because the heat is generated within the tissues, not by external convection 3- proteins (and antigens as proteins themselves) are not adversely affected by MW radiation (or so the say). 4- everybody using MW tissue processing claims that IHC procedures are not affected by the procedure. Having said all of the above I personally do not like MW processing; there are many ways of having fast processing with conventional tissue processors. René J. From: Gudrun Lang gu.l...@gmx.at To: Histonet@lists.utsouthwestern.edu Sent: Monday, January 13, 2014 1:56 PM Subject: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microwave processing plus embedding
Hi! Has anyone heard about a new device for automatic microwave processing and embedding (not the Sakura Xpress) ? It should be a two-bath processor and should use Isopropanol. For about 45 samples per run. Thanks for your responses in advance Gudrun Lang Ltd. BMA histolab Linz, Austria ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave processing. ..... Isopropanol
If your lab does Microwave processing using Isopropanol. How do you disposed this reagent? Thank you. Lin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave processing
Hello, I am a histology technology student and I need information on the melting point of the paraffin used in Microwave Processing… Freida Carson stated in her book something regarding the paraffin temperature set at 84 degrees. The class had a discussion regarding the same and concluded that the tissue morphology would be damage at such high temperature. Can someone please provide me with some input on this issue. Thank you, Jamie G. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microwave processing
Hi there, On an Instrumentation course at keiser, we had a discussion about the right temperature fro the paraffin when the proceesing is done using microwaves. Can some tell me what should be the ideal temperature with out damaging the processing. The text book mentions 84 degrees, but isn't this to high; perhaps if the time is less, it wont affect it? Thanks, Ismael Lopez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microwave processing
I believe that the higher temperature is needed to evaporate off the isopropanol. Just make sure that your tissue is well fixed. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zoe rosa Sent: Monday, 7 November 2011 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processing Hi there, On an Instrumentation course at keiser, we had a discussion about the right temperature fro the paraffin when the proceesing is done using microwaves. Can some tell me what should be the ideal temperature with out damaging the processing. The text book mentions 84 degrees, but isn't this to high; perhaps if the time is less, it wont affect it? Thanks, Ismael Lopez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microwave processing
Does anyone have any experience using an EMS laboratory microwave for processing and/or staining tissue for light or electron microscopy? I need help getting started. Linda Dybas Knox Collee Galeesburg, IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave processing
Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run? We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork! Thanks! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave processing
Dorothy: You should follow for MW processing the same procedure you follow for any traditional tissue processing. What you have to document is that when you started to use the MW processing there was a documented QC to compare the results between the method you used and the new with the MW processor. Having to sign a QC after each run is absolutely unnecessary if the initial QC was done. René J. --- On Thu, 2/3/11, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Subject: [Histonet] Microwave processing To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Thursday, February 3, 2011, 3:38 PM Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run? We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork! Thanks! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave processing effect on DNA/RNA
Hello All, I have seen some posts on the possible damaging effects on DNA and RNA by microwave processing. Does anyone have a reference article that they can share with me? We are in the process of budgeting for next year and we are looking at microwave processors; but I want to verify if this claim is or is not valid. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microwave processing and IHCs
Your control tissue needs to be processed the same as your patient samples. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Monday, March 01, 2010 7:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave processing and IHCs Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave processing and IHCs
Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue. thanks for any help! Michelle _ Hotmail: Free, trusted and rich email service. http://clk.atdmt.com/GBL/go/201469228/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microwave processing
Can someone let me know the recipe for the solution you make up yourself for processing instead of having to purchasing Pro-Wave? By the way, I absolutely love my microwave processor from Milestone! I am a Mohs tech and have now opened up a full path lab within our Dermatology practice. I am very busy but loving every min. of it! Regards, Kathy Hicks H.T.( ASCP ) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
