Some PAS procedures call for sulphurous rinses after the Schiffs reagent. >From your experience is there a noticeable difference if you do not do the rinse? Would it matter if you used sodium metabisulfite rather than potassium metabisulfite? Thank, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet