[Histonet] RE: Eosin Leaching
I too have been experiencing Eosin leaching. I have tried all different options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols and xylenes that follow, even added a running water wash step after the Eosin ( to remove excess). Before this, I have had no problems and had made no changes to my daily activities. I rotate solutions daily. Aside from changing the company I buy my Eosin from, I am at a loss. Thanks in advance for your help. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, June 23, 2014 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Eosin Leaching (Tony Reilly) 2. Histology Benchmarking Survey (Lesley Bechtold) -- Message: 1 Date: Mon, 23 Jun 2014 09:22:28 +1000 From: Tony Reilly tony.rei...@health.qld.gov.au Subject: RE: [Histonet] Eosin Leaching To: Rathborne, Toni toni.rathbo...@rwjuh.edu, Sanders, Jeanine (CDC/OID/NCEZID) j...@cdc.gov, 'AdrienneAnderson' rennie1...@yahoo.com, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: e8088c46632c1c489b85680dd5c0d76b028140c8a...@exccmsp10.qh.health.qld.gov.au Content-Type: text/plain; charset=iso-8859-1 Hi Adrienne Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reil...@health.qld.gov.au | www.health.qld.gov.au ? ? ? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, 20 June 2014 10:11 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histo...@listsutsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] RE: Eosin Leaching
Have you changed your reagent alcohol or has the company you buy from changed where it purchases the reagent alcohol? Reagent alcohols with higher volumes of isopropanol than 5% can cause the eosin to leach out. It will leave a green powder in the bottom of you staining dish. Methanol and Isopropanol should be 5% each with 90% ethanol for the safest blend and what most of us old folks always thought reagent alcohol would stay. We were wrong and different companied can change vendors or sources and get something they were not expecting too. If you look up reagent alcohol or SDA reagent alcohol you find it has many formulas and the only real requirement by the ATF is to make the alcohol non-drinkable or poison to humans. I have seen formulas with 5 to 8% methanol and isopropanol with as low as 87% ethanol. The percentage will depend on price as they have no idea at the manufacturing level what we use ti for or why. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos, Lori (VDH) Sent: Monday, June 23, 2014 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Eosin Leaching I too have been experiencing Eosin leaching. I have tried all different options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols and xylenes that follow, even added a running water wash step after the Eosin ( to remove excess). Before this, I have had no problems and had made no changes to my daily activities. I rotate solutions daily. Aside from changing the company I buy my Eosin from, I am at a loss. Thanks in advance for your help. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, June 23, 2014 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Eosin Leaching (Tony Reilly) 2. Histology Benchmarking Survey (Lesley Bechtold) -- Message: 1 Date: Mon, 23 Jun 2014 09:22:28 +1000 From: Tony Reilly tony.rei...@health.qld.gov.au Subject: RE: [Histonet] Eosin Leaching To: Rathborne, Toni toni.rathbo...@rwjuh.edu, Sanders, Jeanine (CDC/OID/NCEZID) j...@cdc.gov, 'AdrienneAnderson' rennie1...@yahoo.com, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: e8088c46632c1c489b85680dd5c0d76b028140c8a...@exccmsp10.qh.health.qld.gov.au Content-Type: text/plain; charset=iso-8859-1 Hi Adrienne Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reil...@health.qld.gov.au | www.health.qld.gov.au ? ? ? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, 20 June 2014 10:11 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histo...@listsutsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem
[Histonet] RE: Eosin Leaching
Hi Adrienne, We see eosin leaching in our summer months when it is very humid in our lab. The humidity is 70% for most of the summer. We change out all of our alcohols daily to try and prevent it but we sometimes see it even with doing that. Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email rhonda.grego...@gov.mb.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: June-23-14 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Eosin Leaching (Tony Reilly) 2. Histology Benchmarking Survey (Lesley Bechtold) -- Message: 1 Date: Mon, 23 Jun 2014 09:22:28 +1000 From: Tony Reilly tony.rei...@health.qld.gov.au Subject: RE: [Histonet] Eosin Leaching To: Rathborne, Toni toni.rathbo...@rwjuh.edu, Sanders, Jeanine (CDC/OID/NCEZID) j...@cdc.gov, 'AdrienneAnderson' rennie1...@yahoo.com, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: e8088c46632c1c489b85680dd5c0d76b028140c8a...@exccmsp10.qh.health.qld.gov.au Content-Type: text/plain; charset=iso-8859-1 Hi Adrienne Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reil...@health.qld.gov.au | www.health.qld.gov.au ? ? ? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, 20 June 2014 10:11 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histo...@listsutsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list
[Histonet] Re: Eosin leaching out of sections
Hi Scott, Your discription of eosin leaching out after a few days is classic for the possibility of bleach contamination on your staining containers. There are multiple possibilities of other causes, but this could be a simple cause, easily solved. Renee Grow, BA., HT (ASCP) rg...@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an HE stained slide? Thanks, Scott Hendricksen HT(ASCP) ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
