[Histonet] Re: India Ink for inking surgical margin borders
Ed Crespo, CT(ASCP) in Cypress CA asks: I normally purchase India Ink from one of our vendors, but know it's also sold at artist supply shops. Does anyone know if I can use the artist india ink for Pathology? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. I've used india ink from artist supply stores for marking surgical margins, for many years, and it's entirely satisfactory. A cheap source of colored particulate inks is tattoo inks - available in a huge range of colors - I have one pathologist client who's used them for years. The only downside is that you have to read some seriously yucky catalogs. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: India Ink for inking surgical margin borders
Tattoos Yucky? Guess I'm yucky. I got yucky way before tattoos became popular when the response was more akin to fringe element. Happy member of the Fringe. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, October 10, 2013 11:43 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: India Ink for inking surgical margin borders Ed Crespo, CT(ASCP) in Cypress CA asks: I normally purchase India Ink from one of our vendors, but know it's also sold at artist supply shops. Does anyone know if I can use the artist india ink for Pathology? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. I've used india ink from artist supply stores for marking surgical margins, for many years, and it's entirely satisfactory. A cheap source of colored particulate inks is tattoo inks - available in a huge range of colors - I have one pathologist client who's used them for years. The only downside is that you have to read some seriously yucky catalogs. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: India Ink for inking surgical margin borders
I use Artist China ( India ) Ink. The secret is to immerse the blocks in Bouin’s Fixative to coagulate the Ink. After that put the tissue blocks directly in the First Alcohol. Don’t go back to formalin. Cesar Romero Buenos Aires Argentina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: India Ink for inking surgical margin borders
Pam Marcum notes that The only warning I know of (and I have used India Ink) is to be sure it is the permanent India Ink not the washable. If you buy the non-permanent it will come off in processing. India ink is a suspension of carbon black (basically soot) in a suitable vehicle. It's quite permanent - there is no washable India ink. Ask at the art supply store if you're unsure of what you're buying. if you blot the specimen thoroughly dry before you ink it, you don't need fixatives for the ink like acetic acid, acetone, or Bouin fixative. I never use them. Ink won't stick to a cauterized surface (like a LEEP or a lumpectomy specimen) but the pathologist can see those cauterized margins under the microscope anyway. I didn't say tattoos were yucky - I said the catalogs were yucky. But bear with an old man who doesn't think they make girls any more like they did in 1955 (fortunately I've got one). As more and more restrictions are put on the tools grossing pathologists, PAs, and technologists can have, it becomes more important to know how to obtain tools and supplies in the real world. I can't replace my 25 year old Satterlee amputation saw, so I cut fractured femoral heads with a seven dollar hacksaw I bought at Home Depot. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
