[Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides
Dear All: We use a Tupperware container, with a nice air/liquid-tight lid. But any container like that will work. Our trick is to buy plastic ceiling tile material from the hardware store. (Used in ceiling panels.) It comes in big sheets (5 feet by 3 feet?) and is pretty cheap (~$12/sheet). We get the white or clear kind that has square holes cut through the plastic. The holes are about 1 cm square. We then use pliers to cut out panels/pieces that will fit inside the container (wear face protection because the plastic flies all over the place). We put one piece in the bottom of the container, then set a metal slide rack (or any water-proof slide rack) on top. We put water in the bottom and this first panel keeps the slides from getting wet. Then, I cut out 4 single squares of the material and put them inside the metal slide racks, at all 4 corners. Then I can lay another metal slide rack on top. Another 4 pieces, another rack, etc. So you can build up a tall stack depending on the size of your container. And the ceiling tile material is also really good for under drying dishes and other uses around the lab. The cheap way to make an incubation chamber! Sarah Pixley Univ. of Cincinnati ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides
Sarah, Wow, brings back memories of the good ol' days when we used all kinds of stuff to make our own trays. In one lab I worked at we had a small Tupperware container for each antibody and layed paper towels and wood rods on the bottom to hold the slides the surface. We'd wet the paper towels for humidity. When a new lab was built they specifically designed it for this method and we had a 20-foot long bench just so we could line up dozens of these small Tupperware containers! What a pain that was to go through each round of washing!! We did 150 slides a day that way for years. Blessed is the day we got automated stainers!! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pixley, Sarah (pixleysk) Sent: Tuesday, July 09, 2013 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides Dear All: We use a Tupperware container, with a nice air/liquid-tight lid. But any container like that will work. Our trick is to buy plastic ceiling tile material from the hardware store. (Used in ceiling panels.) It comes in big sheets (5 feet by 3 feet?) and is pretty cheap (~$12/sheet). We get the white or clear kind that has square holes cut through the plastic. The holes are about 1 cm square. We then use pliers to cut out panels/pieces that will fit inside the container (wear face protection because the plastic flies all over the place). We put one piece in the bottom of the container, then set a metal slide rack (or any water-proof slide rack) on top. We put water in the bottom and this first panel keeps the slides from getting wet. Then, I cut out 4 single squares of the material and put them inside the metal slide racks, at all 4 corners. Then I can lay another metal slide rack on top. Another 4 pieces, another rack, etc. So you can build up a tall stack depending on the size of your container. And the ceiling tile material is also really good for under drying dishes and other uses around the lab. The cheap way to make an incubation chamber! Sarah Pixley Univ. of Cincinnati ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides
We use bioassay dishes with 10mL pipets glued to them. The slides sit on two pipets and you can get two rows of slides in. Cutting the pipets' ends off is the hardest part. I take that back, finding a glue that will hold the pipets has been a problem lately, as rubber cement isn't made like they used to make it. A glue gun works, but sometimes the pipets need to be reglued. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Tue, Jul 9, 2013 at 1:34 PM, Morken, Timothy timothy.mor...@ucsfmedctr.org wrote: Sarah, Wow, brings back memories of the good ol' days when we used all kinds of stuff to make our own trays. In one lab I worked at we had a small Tupperware container for each antibody and layed paper towels and wood rods on the bottom to hold the slides the surface. We'd wet the paper towels for humidity. When a new lab was built they specifically designed it for this method and we had a 20-foot long bench just so we could line up dozens of these small Tupperware containers! What a pain that was to go through each round of washing!! We did 150 slides a day that way for years. Blessed is the day we got automated stainers!! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pixley, Sarah (pixleysk) Sent: Tuesday, July 09, 2013 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides Dear All: We use a Tupperware container, with a nice air/liquid-tight lid. But any container like that will work. Our trick is to buy plastic ceiling tile material from the hardware store. (Used in ceiling panels.) It comes in big sheets (5 feet by 3 feet?) and is pretty cheap (~$12/sheet). We get the white or clear kind that has square holes cut through the plastic. The holes are about 1 cm square. We then use pliers to cut out panels/pieces that will fit inside the container (wear face protection because the plastic flies all over the place). We put one piece in the bottom of the container, then set a metal slide rack (or any water-proof slide rack) on top. We put water in the bottom and this first panel keeps the slides from getting wet. Then, I cut out 4 single squares of the material and put them inside the metal slide racks, at all 4 corners. Then I can lay another metal slide rack on top. Another 4 pieces, another rack, etc. So you can build up a tall stack depending on the size of your container. And the ceiling tile material is also really good for under drying dishes and other uses around the lab. The cheap way to make an incubation chamber! Sarah Pixley Univ. of Cincinnati ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides
For Manual IHC I suggest best the Sequenza Slide Rack from Thermo Scientific. It makes your life easier and you can save money, because you only need 100 ul of any reactive per slide. Here is the link to the web page. http://www.thermo.fi/com/cda/product/detail/0,1055,105630785,00.html I hope it helps. Cesar Romero Buenos Aires Argentina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
