To all - I am working with NBF fixed paraffin embedded tissue and need to do IMF staining potentially using two different pH retrievals, that are all the same primary host species - I know that if I am doing bright field, I would retrieve, stain for primary 1 with DAB first and the sequentially retrieve and stain for primary 2 with a different chromogen...... any suggestions when working with fluorescence? Does the first reaction get removed from the retrieval for the second epitope; I am in new territory and could use some recommendations..... TIA. Amy
Amy S. Porter, HT (ASCP) - Lab Supervisor Michigan State University Investigative HistoPathology Lab 567 Wilson Road - Rm 2133 East Lansing, MI 48824 Ph: 517-884-5026 "Life is like riding a bicycle. To keep your balance, you must keep moving." - Albert Einstein _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
