Re: [Histonet] Staining issues

[Tony Henwood (SCHN) via Histonet]

Hi Charles,

Many issues could be in play.
Since the centre of the tissue is causing problems, then I would look at 
fixation; are the spaces around cells in the centre of the block wider than at 
the edges (conversely do the cells look like they are smaller than their 
counterparts at the edge)?

If part of this tissue is lightly fixed, they seem to be more prone to heating 
effects. If the haematoxylin is paler (washed-out?) in the centre then it is 
possible that the slides have been heated above 70oC before H staining. This 
will also affect the IPX's. Maybe the oven is over-heating.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, 29 December 2016 12:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Staining issues

Our pathologists are complaining that the center of tissues are not staining 
properly in both our H's and IHC's. Can anyone provide some thoughts as to 
where this problem could be occurring?


As a separate situation we are experiencing the tissues folding or falling off 
during the staining process. Both this issue and the staining issue are recent 
occurrences and we have not changed our process in any way from previous years.


Does anyone think these problems are related? Why or why not?   I have
racked my brain with all the trouble shooting techniques I can think of so any 
help will greatly be appreciated

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] Staining issues

[Rene J Buesa via Histonet]

Improper staining at the center and falling sections are typical consequences 
of poor fixation/infiltration.If you have changed nothing proceduraly, what 
about somebody "new" grossing and preparing thicker tissue slices?René 

On Wednesday, December 28, 2016 8:34 AM, Charles Riley via Histonet 
 wrote:
 

 Our pathologists are complaining that the center of tissues are not
staining properly in both our H's and IHC's. Can anyone provide some
thoughts as to where this problem could be occurring?


As a separate situation we are experiencing the tissues folding or falling
off during the staining process. Both this issue and the staining issue are
recent occurrences and we have not changed our process in any way from
previous years.


Does anyone think these problems are related? Why or why not?  I have
racked my brain with all the trouble shooting techniques I can think of so
any help will greatly be appreciated

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] Staining issues

[Charles Riley via Histonet]

Our pathologists are complaining that the center of tissues are not
staining properly in both our H's and IHC's. Can anyone provide some
thoughts as to where this problem could be occurring?


As a separate situation we are experiencing the tissues folding or falling
off during the staining process. Both this issue and the staining issue are
recent occurrences and we have not changed our process in any way from
previous years.


Does anyone think these problems are related? Why or why not?   I have
racked my brain with all the trouble shooting techniques I can think of so
any help will greatly be appreciated

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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