[Histonet] bone marrow aspirate slides for iron stains
Try hydrating the slide in 95% then distilled before staining. The iron stain is made in water so hydrating to water should help the stain transfer better. Hope this helps :) Cassandra Davis cda...@che-east.org 302-575-8095 Saint Francis Hospital Saintfrancishealthcare.org Saint Francis Facebook Page Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone marrow aspirate iron stain
Hello, We are currently having problems with our bone marrow aspirate slides for iron stains which we run on the Ventana nexes special stainer. The RBCs look crenated or damaged. We currently fix them 5 minutes in methanol then air dry before running them on the stainer. Any suggestions? Jason Renz HT(ASCP)/lab safety officer St. Alexius Medical Center 900 East Broadway Bismarck ND, 58506 (701)530-6733 This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bone marrow aspirate
Does anyone have a protocol on how to fix and process a bone marrow aspirate to paraffin? Thanks, Tim Coskran Pfizer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bone marrow aspirate
Hi Tim, The best way I have found over the years actually requires the person collecting the specimen to do the most work. What we used to do is after the aspirate is performed, make all of the smears, and then inject the remaining aspirate directly into formalin before it coagulates. This gets rid of all the blood and ensures all that is left is marrow. After sufficient time in formalin, filter the marrow out of the formalin and process. As for a processing protocol, we do a run as follows: Formalin 30 min 70% Alcohol 20 min 90% Alcohol 10 min 100% Alcohol 10 min 100% Alcohol 10 min 100% Alcohol 15 min Xylene1 15 min Xylene2 15 min Xylene3 20 min Paraffin1 15 min Paraffin2 15 min Paraffin3 30 min This protocol was done with pressure/vacuum. We have excellent results with this and the pathologists do not have to spend a lot of time hunting for small areas of marrow on the slide, the whole slide is marrow. Good luck! Ashley Message: 5 Date: Fri, 4 May 2012 16:54:57 + From: Coskran, Timothy M timothy.m.cosk...@pfizer.commailto:timothy.m.cosk...@pfizer.com Subject: [Histonet] bone marrow aspirate To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Message-ID: 70249e5b79afeb48a47d78568ce216e9027...@ndhamrexde02.amer.pfizer.commailto:70249e5b79afeb48a47d78568ce216e9027...@ndhamrexde02.amer.pfizer.com Content-Type: text/plain; charset=us-ascii Does anyone have a protocol on how to fix and process a bone marrow aspirate to paraffin? Thanks, Tim Coskran Pfizer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
