[Histonet] kappa and lambda by CISH
Hello All, Anyone currently running Kappa and Lambda by CISH? I am in the process of validating the Ventana products. Due to the ASR status of these products the vendor is extremely 'tight lipped'. I have the stains worked up and have completed a 25 case validation. Does anyone know if this test is not to be used on decaled specimens?? Thank you The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] kappa and lambda by CISH
I've found, that kappa and lambda mRNA CISH is reliable for specimen decalcified with 5-10% formic acid. Decal with harsher acids like hydrochloric acid should be validated, because any acid will cause degradation of nucleic acids. We use the CISH kit from Zytovision. Journal of Histotechnol 33(1):9-13, 2010 Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Kuhnla, Melissa Gesendet: Dienstag, 19. Oktober 2010 16:50 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] kappa and lambda by CISH Hello All, Anyone currently running Kappa and Lambda by CISH? I am in the process of validating the Ventana products. Due to the ASR status of these products the vendor is extremely 'tight lipped'. I have the stains worked up and have completed a 25 case validation. Does anyone know if this test is not to be used on decaled specimens?? Thank you The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] kappa and lambda by CISH
Hi Melissa, We use it on bone marrow biopsies all the time on the Ventana XT. We made sure to include BMBXs in the validation. We do get strange staining from time to time, so it's particularily important to look at the negative control for these. Mark Tarango On Tue, Oct 19, 2010 at 7:49 AM, Kuhnla, Melissa melissa.kuh...@chsli.orgwrote: Hello All, Anyone currently running Kappa and Lambda by CISH? I am in the process of validating the Ventana products. Due to the ASR status of these products the vendor is extremely 'tight lipped'. I have the stains worked up and have completed a 25 case validation. Does anyone know if this test is not to be used on decaled specimens?? Thank you The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
