[Histonet] ThinPrep staining
Hi Charles, The only concern mentioned was the intensity of and distribution of the OG. OG and EA are interdependent in the pap stain and it may just be a matter of adjusting the times for OG and EA. A good book on the topic that I recommend is "Cytopreparation, Principles and Practice" by Gary Gill. It contains an entire chapter dedicated to the pap stain. https://www.amazon.com/Cytopreparation-Principles-Practice-Essentials-Cytopathology/dp/1461449324 Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thinprep staining
The pathologists at my office keep asking me to fix the thin prep staining. This is not part of my expertise as I was only given about 10 minutes worth of cytoprep training in school. If anyone out there can offer any assistance for the problem it would be greatly appreciated. Here is what they have said is occurring: "The Orange G staining is too light and inconsistently distributed across the slide. I have also noticed some perinuclear artifacts such as perinuclear clearing." Thanks in advance for your help -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ThinPrep
Does anyone know a CAP regulation for validation of ThinPrep? Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thinprep
We want to purchase a latest model machine for thin prep/sure path along with an auto stainer for pap.stain. Can you share your experience regarding both types of machines, 1) Which type of system is best 2)which machine will provide an auto stainer 3)which model of the recommended machine is best 4)will they arrange for training of cytotechnologist and pathologist for processing and reportting of specimen 5)which company is providing best after sale service Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ThinPrep 2000
We are looking into using a ThinPrep 2000 instrument for our urine cytologies. A rep from Hologic told my manager these can also be used to do FISH on urines as well. I am wondering if anyone uses the ThinPrep 2000 for this purpose and if so, how well do you like it? Thanks, Bryan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ThinPrep 2000
I know of a lab where I work sometimes (on call) they use thin prep 2000 and do the fish studies on the sample. I think it's working for them. The lab name is Uro partners. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Thursday, August 04, 2011 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ThinPrep 2000 We are looking into using a ThinPrep 2000 instrument for our urine cytologies. A rep from Hologic told my manager these can also be used to do FISH on urines as well. I am wondering if anyone uses the ThinPrep 2000 for this purpose and if so, how well do you like it? Thanks, Bryan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ThinPrep
Does anyone know if the ThinPrep system has a reagent rental program available, rather than just purchasing the instrument? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ThinPrep vs. SurePath
Hi Jennifer, I have used both systems, as a cytotech, processing tech, and as a supervisor. As a cytotech, there is less area of the slide to have to review so from that viewpoint it seems more efficient. The drawback for me was the size of the cells. For Gyn specimens, very small high grade cells can be difficult to detect without some sort of computer assisted system. The Focal Point system was supposed to provide this assistance but I found it somewhat unreliable. This was further complicated by the Focal Point being about 50% accurate in detecting endocervical cells. Labs that allowed specimens that automatically signed out cases the Focal Point deemed at the lowest risk for abnormal cells frequently had clinicians repeating paps due to there being no endocervical material detected. I found that non-gyn specimens processed using SurePath were very difficult for inexperienced techs and many pathologists simply did not trust that they were getting the appropriate sampling of cells. Very experienced cytotechs and some pathologists preferred SurePath for non-gyns but they are in the minority and I found them difficult and time consuming to review. As a processing tech, SurePath was very time consuming and if a lab has any kind of volume at all, had to have at least 2 technicians dedicated to nothing but processing them. ThinPrep has a larger are to look at and those techs who are used to SurePath do not like to review them because the cells are quite a bit larger, and if doing manual screening, takes a bit longer to review. Use of the ThinPrep Imager has cut screening time down considerably. Time and statistical analysis has shown that the Imager is a very reliable instrument for indicating those areas of the slide where abnormal cells may be found. Criticism has been that the Imager sometimes misses cells with HPV and viral effect. Even so, the primary focus of many labs is to properly detect and report high grade lesions and for this the Imager is reliable. For me, non-gyn specimens were easier to review and displayed a good distribution of cells. A good many pathologists agree and are confident that the cell sampling adequate. As a processing tech, ThinPrep processing takes less time and less personnel than SurePath. If the T-5000 processor is ever released by the FDA for use in the US for gyn specimens, this time will be further reduced. In the end, the smaller cell distribution size offered by SurePath, while seemingly more efficient, requires more time to review for many techs due to the small size of the cells. ThinPrep currently offers a better solution to a majority of labs in terms of accuracy, time to prepare, and Imaging. Hope this was helpful. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, November 10, 2010 6:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ThinPrep vs. SurePath Does anyone have an opinion as to why there seems to be many more ThinPreps out there than Sure Path? The SurePath seems more efficient, but I might be missing something. Thank you, Jennifer MacDonald ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ThinPrep vs. SurePath
Does anyone have an opinion as to why there seems to be many more ThinPreps out there than Sure Path? The SurePath seems more efficient, but I might be missing something. Thank you, Jennifer MacDonald ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
