I use a platform shaker and slosh the sample around while it is in decal solution at RT, just make sure it is well fixed before you decal, Immunocal is 5% formic acid decal solution it is not a fixative and it is a bit slower than some of the rapid decal reagents out there but is much better for IHC. When I was in HemePath we would fix for about 6 hrs before decaling on the shaker for 2-4hrs depending on the size (thickness) of the bone bx.
Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net > Date: Mon, 17 Nov 2014 14:05:28 -0500 > From: carolyn.barn...@va.gov > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Bone Marrow processing using Immunocal > > I am looking for a Bone Marrow procedure that uses Immunocal as the > decalcifier. I am particularly interested in how long they fix in > Immunocal and if heat is used in any way . Our lab just started using > this product because we are under the impression that it gave better ISH > results. > > Thank-you all so much for any assistance. I really appreciate everyone's > help. > > > > Carolyn K. Barnes > > Histology Supervisor > > McGuire VA Medical Center > > Richmond, VA 23249 > > Ph: 804-675-5000, x2158 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
