RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block
How many are we talking about? I embed 6 sections of mouse = bowel on
end and it works? Just fill the mold, put it on the cold spo= t for a
second, then on some room temperature area, the paraffin will harde= n
slowly enough that you should be able to embed them?
Sarah Goebel, B.A., = HT (ASCP)
Histotechnician
= /div
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, T= exas 78744
(512)386-5107
= br
Original Message
Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin
block
From: [1]kgrob...@rci.rutgers.ed= u
Date: Fri, July 16, 2010 8:23 am
To: histonet [2]h= isto...@lists.utsouthwestern.edu
Is there a way to do this without one or more pieces falling over? I
saw in the archive the method for frozen sections-embed them on their
sides in= br OCT, then cut on the end, but I don't think I'd be able
to do that in
paraffin. Would one of the tissue microarray methods work? (I've never
done that before, so I have no idea.)
Thanks in advance for all your help,
Kathleen
Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(732) 445-6914
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References
1. 3Dmailto://kgrob...@rci.rutgers.edu/
2. 3Dmailto://histonet@lists.utsouthwestern.edu/
3. 3Dmailto://Histonet@lists.utsouthwestern.edu/
4. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block
I have techs that embed 10-15 pieces on end in one block. Just cool the block slowly and move your pieces around quickly. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kgrob...@rci.rutgers.edu Sent: Friday, July 16, 2010 10:24 AM To: histonet Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin block Is there a way to do this without one or more pieces falling over? I saw in the archive the method for frozen sections-embed them on their sides in OCT, then cut on the end, but I don't think I'd be able to do that in paraffin. Would one of the tissue microarray methods work? (I've never done that before, so I have no idea.) Thanks in advance for all your help, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embedding multiple GI pieces on end in a paraffin block
We regularly embed 6-12 pieces on end in one block without any special method. You just have to be quick... and don't leave the block on the cold plate very long... just touch the cold plate briefly while embedding the individual piece, then lift the block off the plate until you grab the next piece... repeat quickly and you'll run out of room in the mold before you'll have to worry about it hardening too much. Drew On Fri, Jul 16, 2010 at 11:23, kgrob...@rci.rutgers.edu wrote: Is there a way to do this without one or more pieces falling over? I saw in the archive the method for frozen sections-embed them on their sides in OCT, then cut on the end, but I don't think I'd be able to do that in paraffin. Would one of the tissue microarray methods work? (I've never done that before, so I have no idea.) Thanks in advance for all your help, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block
To all, OK, looks like most of you are saying the same thing-work fast! :o) I've printed out all of your replies and discussed them with the graduate student who needs this done for her thesis, and she agrees with me-it's just going to take practice. I'll try the cool slowly/work fast suggestions first and see how that goes, then see about the trick with the cucumber (which sounds really cool!), slowly edging the mold onto the cold plate as I go, and using sponges to help flip the samples on end (and not necessarily in that order). I don't want to try too many things at once, lest I drive myself nuts. The first batch of samples should be coming sometime next week. Wish me luck, and thanks for all your help! Kathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block
Hello, The way I used to embed GI specimens is to place them on end on a stack of specimen bags that have solidified, since they are already in place, then you can pick them up quickly. I did this with sectioned arterial artieries or vas deferens this worked like a dream. Robyn Vazquez Date: Fri, 16 Jul 2010 11:00:01 -0500 From: mpe...@grhs.net To: kgrob...@rci.rutgers.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block CC: I have techs that embed 10-15 pieces on end in one block. Just cool the block slowly and move your pieces around quickly. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kgrob...@rci.rutgers.edu Sent: Friday, July 16, 2010 10:24 AM To: histonet Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin block Is there a way to do this without one or more pieces falling over? I saw in the archive the method for frozen sections-embed them on their sides in OCT, then cut on the end, but I don't think I'd be able to do that in paraffin. Would one of the tissue microarray methods work? (I've never done that before, so I have no idea.) Thanks in advance for all your help, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
