TBS has good dyes...you can mordant them (what Bouins does) in fo rmalin-aceto-alcohol. This is less toxic then Bouins and works well.&= nbsp; Of course if you are doing anything with breast tissue, you don't wan= t to leave it in there but a second or two because it is not known if it ef= fects ER/PR results.
Happy Fixing!! PS-W= e called F-A-A "juicy juice" because that's what it smells like =) Sarah = Goebel, B.A., HT (ASCP) Histotechnician <= div> XBiot= ech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 [DEL: (512)386-5107 :DEL] -------- Original Message -------- Subject: [Histonet] RE: embedding beads/tissue marking dyes From: Maria Katleba <[1]Maria.Ka= tl...@stjoe.org> Date: Tue, October 05, 2010 2:40 pm To: Jacqueline Farnsworth <[2]jacqueline.farnswo...@cls.ab.ca>, "[3]histo...@lists.utsout= hwestern.edu" <[4] Histonet@lists.utsouthwestern.edu> When you place dye on any tissue, make sure you pipette some bouins on to t= he tissue... Apparently the bouins "sets" the stain... afterwards dab the t= issue with paper towel to sop up the excess dye/bouins. Some dyes are better than others, but try the bouins first Call me or email me directly, I can help you with details :) Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: [5]histonet= -boun...@lists.utsouthwestern.edu [[6]mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jacqueline Farnsworth Sent: Tuesday, October 05, 2010 2:14 PM To: [7]histo...@lists.uts= outhwestern.edu Subject: [Histonet] embedding beads/tissue marking dyes Hi We may be encountering some issues with our tissue marking dyes 'clogging' = up our processors. I"ve heard of a system where a coloured 'bead' is embed= ded right beside the tissue, and subsequently cut onto the slide. This doe= s not mark the margins obviously, but is used as a method to track like spe= cimens that are grossed, embedded and subsequently cut in a row. (red, oran= ge, green, blue.....) I explored the archives for "embedding beads", but only found reference to = a glass bead that is placed in the wax inside the block, but not subsequent= ly cut. PS: we are still troubleshooting our dyes (dilutions, brand, etc.), but if = anyone has a brand of dye that they like and have no issues with, I'd be th= rilled to get the information as well! Thanks in advance, Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Diagnostic Scientific Centre Calgary Laboratory Services Phone: 403-770-3588 Pager: 403-212-8223 X07630 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intende= d recipient(s), are confidential and may contain privileged information. An= y unauthorized review, use, retransmission, or other disclosure is strictly= prohibited. If you have received this message in error, please notify the = sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list [8]histo...@lists.utsouth= western.edu [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privilege= d and confidential and protected from disclosure. _______________________________________________ Histonet mailing list [10]histo...@lists.utsouth= western.edu [11]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:maria.katl...@stjoe.org"; 2. 3D"mailto:jacqueline.farnswo...@cls.a 3. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 4. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 5. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu"; 6. 3D"mailto:histonet-boun...@l 7. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 8. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; 10. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
