I just did this last week. I stored them in wash buffer overnight in the frig. and they were fine =)
Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stacy Deppeler Sent: Wednesday, March 16, 2011 3:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storing slides in buffer Hi guys, I am wondering if anyone has much experience storing slides in buffer (PBS-T) after retrieval for up to about 48hrs at 4C? Is it likely that they will still stain? Or is it going to be entirely dependent on the epitope? I'm optimizing research antibodies right now and I'd like to decrease my turn around time by knocking off the HIER at an earlier time point. Thanks. -- Stacy Deppeler. Research Assistant Department of Ophthalmology King Abdulaziz University Hospital Riyadh, KSA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet