Re: [Histonet] Cryostat anti-roll plate
> > > My experience with anti-roll plates is mostly with the old International > cryostats of days gone by. Pathologists usually disdained anti-roll plates > and used an artist's brush to keep the section from rolling, but more > proficient users depended on them. I learned to use them in a research > histology lab. We sat at the side of the cryostat by the crank, rather than > in front of it, and used an anti-roll plate. It was easy to cut fifty > slides with it, which I required for my research fifty years ago. Bob Richmond retired samurai pathologist Maryville TN > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat anti-roll plate
Got it... I need to test it. But, I'm going to take some parafilm, pull it over the bolt, then replace the screw. Increase that friction. Geri -Original Message- From: Keyser, Geri L via Histonet Sent: Wednesday, May 25, 2022 9:59 AM To: Anne Murvosh ; Ken Marzinsky ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryostat anti-roll plate Maybe there's a product that increases friction with screw threads, doesn't have toxic outgassing, and works at low temperatures. I'm going to look for something like this. Geri -Original Message- From: Anne Murvosh via Histonet Sent: Wednesday, May 25, 2022 8:32 AM To: Ken Marzinsky ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryostat anti-roll plate WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. We have 3 new leicas and we constantly have to adjust them. They creep forward so much that I just automatically know to roll it slightly back after every 4 specimens or so. The new leica designs are just bad and customer service is even worse. We have to use the plate as we do Mohs specimens and the sections are just better with the glass plate. I want to look for a different machine next time. We did test the Avantik and it cut nice but the glass plate was on the front and got in the way. I wish they would talk to Mohs techs before they design these. Oh well. No tricks just constant adjusting. Thanks Anne -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time? I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle. Ken Durham, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=05%7C01%7Camurvosh%40advancederm.net%7Cd00ec7fb978f48d5784b08da3dc25292%7Cc026b5a4e9b54bdfb53b7130122cdb95%7C0%7C0%7C637890202080603264%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7Csdata=Uhz9rhvCBTV95t%2BNEs0ocizQBXV7aAQyxswaE2Thm2E%3Dreserved=0 HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat anti-roll plate
Maybe there's a product that increases friction with screw threads, doesn't have toxic outgassing, and works at low temperatures. I'm going to look for something like this. Geri -Original Message- From: Anne Murvosh via Histonet Sent: Wednesday, May 25, 2022 8:32 AM To: Ken Marzinsky ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryostat anti-roll plate WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. We have 3 new leicas and we constantly have to adjust them. They creep forward so much that I just automatically know to roll it slightly back after every 4 specimens or so. The new leica designs are just bad and customer service is even worse. We have to use the plate as we do Mohs specimens and the sections are just better with the glass plate. I want to look for a different machine next time. We did test the Avantik and it cut nice but the glass plate was on the front and got in the way. I wish they would talk to Mohs techs before they design these. Oh well. No tricks just constant adjusting. Thanks Anne -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time? I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle. Ken Durham, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=05%7C01%7Camurvosh%40advancederm.net%7Cd00ec7fb978f48d5784b08da3dc25292%7Cc026b5a4e9b54bdfb53b7130122cdb95%7C0%7C0%7C637890202080603264%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7Csdata=Uhz9rhvCBTV95t%2BNEs0ocizQBXV7aAQyxswaE2Thm2E%3Dreserved=0 HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat anti-roll plate
We have 3 new leicas and we constantly have to adjust them. They creep forward so much that I just automatically know to roll it slightly back after every 4 specimens or so. The new leica designs are just bad and customer service is even worse. We have to use the plate as we do Mohs specimens and the sections are just better with the glass plate. I want to look for a different machine next time. We did test the Avantik and it cut nice but the glass plate was on the front and got in the way. I wish they would talk to Mohs techs before they design these. Oh well. No tricks just constant adjusting. Thanks Anne -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time? I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle. Ken Durham, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=05%7C01%7Camurvosh%40advancederm.net%7Cd00ec7fb978f48d5784b08da3dc25292%7Cc026b5a4e9b54bdfb53b7130122cdb95%7C0%7C0%7C637890202080603264%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7Csdata=Uhz9rhvCBTV95t%2BNEs0ocizQBXV7aAQyxswaE2Thm2E%3Dreserved=0 HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat anti-roll plate
I use roll plate. I don't like the roll plate on the Leica. The Leica design creeps... a lot. Also, with the leica, the contact point with the blade is a soft aluminum arm. If you make adjustments with the roll plate holder contacting the blade, it will damage the soft aluminum holder. I like the thermo design much better. It creeps a lot less. Here's a procedure for roll plate adjustment. 1. Mount block on specimen disc. 2. Place Specimen Disc in chuck, position Blade carrier, and face off. 3. Turn knurling screw clockwise until sections do not pass under the Anti-roll plate. 4. Turn knurling screw counter-clockwise by 20 degree increments until sections effortlessly pass under the Anti-Roll Plate WITHOUT hitting the block. * If the block is hitting the anti-roll plate, it may cause damage to glass. * Hard contact is visible and can be heard. * Soft contact, which can also damage the Anti-roll plate, cannot be seen or heard. But, can be felt by gently placing finger on the anti-roll plate turning lever while taking a section. Geri -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time? I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle. Ken Durham, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat validation
Tony - We were on the same page. We purchased fresh beef liver, sectioned and froze it, cut and stained duplicate blocks and had the pathologist sign off on the process. We were also able to get some tonsils delivered fresh and did them, too. Terri Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 Today's Topics: 2. Re: New cryostat validation procedure (Tony Henwood (SCHN)) Message: 2 Date: Mon, 23 Aug 2021 20:31:19 + From: "Tony Henwood (SCHN)" To: Greg Dobbin Subject: Re: [Histonet] New cryostat validation procedure Hi Greg, I would just grab some kidneys from the butcher, freeze and section as usual. They should give equivalent results to the older unit. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat
I agree with Terri. I also have an 1850 and will use it until parts are no longer available which could be,. like she said, years. We also have a local company do the PM and repairs and will use it until it absolutely cannot be repaired. Colleen Forster HT(ASCP)QIHC University of Minnesota BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 555455 612-626-1930 On Tue, Aug 10, 2021 at 10:47 AM Terri Braud via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I'm not sure I understand why you would want to replace your 1850s just > because Leica is closing the support. I have 2 and they are real > workhorses. There are 2 independent companies in the area that can repair > and perform PMs. One is Bel-Air and the other is Advantik. I'm sure there > are others, but I've used both companies and can highly recommend them. If > you like your Leica, keep it until parts are no longer available, which > could be years. > I have two 1850s and I'm sure they will be here for a long time. Terri > > Terri L. Braud, HT(ASCP) > HNL Laboratories for > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3689 > Fax: 215-938-3874 > Today's Topics: > >1. cryostat (Histology) > > Date: Mon, 9 Aug 2021 13:00:30 + > Subject: [Histonet] cryostat > Hello, > Trying to get everyone's opinion on cryostats. We have had the Leica > CM1850 forever and it is not being supported by Leica anymore. Thinking of > trying a different company maybe Tanner or Rankin. What does everyone use > and like. We do not cut Mohs, only DIFs. Maybe 1 a day. > Thanks to all, > Mehndi Helgren > Dominion Pathology Laboratories > 733 Boush Street > Suite 200 > Norfolk, VA 23510 > 757-664-7901 > > > > -- > > Message: 2 > Date: Mon, 9 Aug 2021 13:56:42 + > From: "Morken, Timothy" > To: Histology > Cc: Histonet > Subject: Re: [Histonet] cryostat > Message-ID: > < > co1pr05mb8053d300dfe09b8f30e66acbe7...@co1pr05mb8053.namprd05.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We > have two Epredia NX70 cryostats. We've used them for 8 years now and really > like them. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies Department > of Pathology UC San Francisco Medical Center > > > -Original Message- > From: Histology via Histonet > Sent: Monday, August 09, 2021 6:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cryostat > > Hello, > > Trying to get everyone's opinion on cryostats. We have had the Leica > CM1850 forever and it is not being supported by Leica anymore. Thinking of > trying a different company maybe Tanner or Rankin. What does everyone use > and like. We do not cut Mohs, only DIFs. Maybe 1 a day. > > Thanks to all, > > Mehndi Helgren > > Dominion Pathology Laboratories > 733 Boush Street > Suite 200 > Norfolk, VA 23510 > 757-664-7901 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!8z84Bh8A8kSfaOqndOJm4QoOseISNw1HiCiz-OksuM_qvkfAVIH4wUOs3iM9HGD5XluxSg$ > > > > -- > > Subject: Digest Footer > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- > > End of Histonet Digest, Vol 213, Issue 6 > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat
I'm not sure I understand why you would want to replace your 1850s just because Leica is closing the support. I have 2 and they are real workhorses. There are 2 independent companies in the area that can repair and perform PMs. One is Bel-Air and the other is Advantik. I'm sure there are others, but I've used both companies and can highly recommend them. If you like your Leica, keep it until parts are no longer available, which could be years. I have two 1850s and I'm sure they will be here for a long time. Terri Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 Today's Topics: 1. cryostat (Histology) Date: Mon, 9 Aug 2021 13:00:30 + Subject: [Histonet] cryostat Hello, Trying to get everyone's opinion on cryostats. We have had the Leica CM1850 forever and it is not being supported by Leica anymore. Thinking of trying a different company maybe Tanner or Rankin. What does everyone use and like. We do not cut Mohs, only DIFs. Maybe 1 a day. Thanks to all, Mehndi Helgren Dominion Pathology Laboratories 733 Boush Street Suite 200 Norfolk, VA 23510 757-664-7901 -- Message: 2 Date: Mon, 9 Aug 2021 13:56:42 + From: "Morken, Timothy" To: Histology Cc: Histonet Subject: Re: [Histonet] cryostat Message-ID: Content-Type: text/plain; charset="us-ascii" Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We have two Epredia NX70 cryostats. We've used them for 8 years now and really like them. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Histology via Histonet Sent: Monday, August 09, 2021 6:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat Hello, Trying to get everyone's opinion on cryostats. We have had the Leica CM1850 forever and it is not being supported by Leica anymore. Thinking of trying a different company maybe Tanner or Rankin. What does everyone use and like. We do not cut Mohs, only DIFs. Maybe 1 a day. Thanks to all, Mehndi Helgren Dominion Pathology Laboratories 733 Boush Street Suite 200 Norfolk, VA 23510 757-664-7901 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!8z84Bh8A8kSfaOqndOJm4QoOseISNw1HiCiz-OksuM_qvkfAVIH4wUOs3iM9HGD5XluxSg$ -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- End of Histonet Digest, Vol 213, Issue 6 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat
Hi Mehndi, Same here, I had been using Epredia cryostat for the last several years. I like it very much. A video on youtube shows its beautiful features. https://youtu.be/oMNt6RT5cWI Best, Haiyan On Mon, Aug 9, 2021 at 7:04 AM Morken, Timothy via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We > have two Epredia NX70 cryostats. We've used them for 8 years now and really > like them. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > -Original Message- > From: Histology via Histonet > Sent: Monday, August 09, 2021 6:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cryostat > > Hello, > > Trying to get everyone's opinion on cryostats. We have had the Leica > CM1850 forever and it is not being supported by Leica anymore. Thinking of > trying a different company maybe Tanner or Rankin. What does everyone use > and like. We do not cut Mohs, only DIFs. Maybe 1 a day. > > Thanks to all, > > Mehndi Helgren > > Dominion Pathology Laboratories > 733 Boush Street > Suite 200 > Norfolk, VA 23510 > 757-664-7901 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!8z84Bh8A8kSfaOqndOJm4QoOseISNw1HiCiz-OksuM_qvkfAVIH4wUOs3iM9HGD5XluxSg$ > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat
Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We have two Epredia NX70 cryostats. We've used them for 8 years now and really like them. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Histology via Histonet Sent: Monday, August 09, 2021 6:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat Hello, Trying to get everyone's opinion on cryostats. We have had the Leica CM1850 forever and it is not being supported by Leica anymore. Thinking of trying a different company maybe Tanner or Rankin. What does everyone use and like. We do not cut Mohs, only DIFs. Maybe 1 a day. Thanks to all, Mehndi Helgren Dominion Pathology Laboratories 733 Boush Street Suite 200 Norfolk, VA 23510 757-664-7901 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!8z84Bh8A8kSfaOqndOJm4QoOseISNw1HiCiz-OksuM_qvkfAVIH4wUOs3iM9HGD5XluxSg$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat
Hi Brittany, Personally I do not like the HM525NX. It is awkward and difficult to control with no manual advance except the control panel. When advancing /trimming you are never certain how far forward the block will travel. The UV light fitting in the lid always seems to get in the way of my field of view. Ours doesn't have temperature control of the block holder which can make sectioning more difficult. Looking at the specs of the cryostar I would actually opt for the NX70. Kind regards, Penny :) Penny Marr Senior BMS C/- Histology Conquest Hospital St Leonards-on-Sea TN37 7RD penny.m...@nhs.net 0300 131 4500 ext 734914 0300 131 4914 -Original Message- From: Brittany Hethcox [mailto:bheth...@hcmhcares.org] Sent: 19 May 2021 15:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Hello everyone! I am new to the Histology world. You all may know my previous coworker, Rhonda Ford. She officially retired and I am so happy for her! With that being said, I am in a pickle because the cryostat we had completely broke down. We were having issues with it during my training period, so I never officially got to use it. We are now looking for a new cryostat for our department. My supervisor is asking me about different models and features, but I don't know much about any of this. So I was hoping you all could help! Here are the details: We are looking at two different models: - HM525NX - CRYOSTAR NX50 We are also getting questions about UV versus fog mist (More specifically the "Cold D" fog mist). So my questions are: 1) Which model do you think is a better investment? 2) Is it ok to buy used? - It's about half the price, which my supervisor really appreciates! - A new cryostat will have to come from overseas. The seller says used ones will probably be available in the states. 3) Which is better, UV or fog mist? 4) Are there any other features that we should aim to include in our purchase (e.g. height adjustment, vacutome, etc.)? Thanks so much for your help! -- Brittany Hethcox Histology/Pathology 765-521-1284 Henry Community Health -- *"We Make Lasting Connections"*Henry Community Health This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in relation to its contents. To do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland. NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and other accredited email services. For more information and to find out how you can switch, https://portal.nhs.net/help/joiningnhsmail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat
Guess I missed your other questions. I think the fog mist (Cold D) may be better - it reaches all area of the chamber. UV light is shaded from some areas, though probably hits most areas you might be touching. The misting requires a specific solution, not just anything. Hydrogen peroxide is the disinfecting agent. Other features: We have the vacutome on the original units but we never use it. We do all kidney and muscle sectioning and these small samples do not produce much debris. On our new replacement unit (to replace a 7-year old NX70) we opted out of the vacuum. If using in a gross room/Or setting the vacuum may be very useful. It only adds a hose and suction manifold in the unit and does not get in the way of anything. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Morken, Timothy via Histonet Sent: Wednesday, May 19, 2021 8:26 AM To: Brittany Hethcox Cc: Histonet Subject: Re: [Histonet] Cryostat Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're reliable and easy to use. We have the elevator on ours and it makes a difference for different size people in the lab. It also can have a vapor disinfectant system which we use and it works great. We use ours for kidney and muscle cryosectioning, not gross room/OR sections but these would work fine for that. They have a lot of room inside for specimens. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Brittany Hethcox via Histonet Sent: Wednesday, May 19, 2021 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Hello everyone! I am new to the Histology world. You all may know my previous coworker, Rhonda Ford. She officially retired and I am so happy for her! With that being said, I am in a pickle because the cryostat we had completely broke down. We were having issues with it during my training period, so I never officially got to use it. We are now looking for a new cryostat for our department. My supervisor is asking me about different models and features, but I don't know much about any of this. So I was hoping you all could help! Here are the details: We are looking at two different models: - HM525NX - CRYOSTAR NX50 We are also getting questions about UV versus fog mist (More specifically the "Cold D" fog mist). So my questions are: 1) Which model do you think is a better investment? 2) Is it ok to buy used? - It's about half the price, which my supervisor really appreciates! - A new cryostat will have to come from overseas. The seller says used ones will probably be available in the states. 3) Which is better, UV or fog mist? 4) Are there any other features that we should aim to include in our purchase (e.g. height adjustment, vacutome, etc.)? Thanks so much for your help! -- Brittany Hethcox Histology/Pathology 765-521-1284 Henry Community Health -- *"We Make Lasting Connections"*Henry Community Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!74SoiCnESMaSi9f-mZZu3TuQpthIBblOoUALh7lOXnMsH4PDJqAkjJJaOlLf5219UuzvTw$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!_ds9AtFEC5PZbUsRGfDv3ncG8lFW-7mf6aFJqWETo4wBI602GQBADwNr2eFqLbYJXlGeDw$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat
Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're reliable and easy to use. We have the elevator on ours and it makes a difference for different size people in the lab. It also can have a vapor disinfectant system which we use and it works great. We use ours for kidney and muscle cryosectioning, not gross room/OR sections but these would work fine for that. They have a lot of room inside for specimens. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Brittany Hethcox via Histonet Sent: Wednesday, May 19, 2021 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Hello everyone! I am new to the Histology world. You all may know my previous coworker, Rhonda Ford. She officially retired and I am so happy for her! With that being said, I am in a pickle because the cryostat we had completely broke down. We were having issues with it during my training period, so I never officially got to use it. We are now looking for a new cryostat for our department. My supervisor is asking me about different models and features, but I don't know much about any of this. So I was hoping you all could help! Here are the details: We are looking at two different models: - HM525NX - CRYOSTAR NX50 We are also getting questions about UV versus fog mist (More specifically the "Cold D" fog mist). So my questions are: 1) Which model do you think is a better investment? 2) Is it ok to buy used? - It's about half the price, which my supervisor really appreciates! - A new cryostat will have to come from overseas. The seller says used ones will probably be available in the states. 3) Which is better, UV or fog mist? 4) Are there any other features that we should aim to include in our purchase (e.g. height adjustment, vacutome, etc.)? Thanks so much for your help! -- Brittany Hethcox Histology/Pathology 765-521-1284 Henry Community Health -- *"We Make Lasting Connections"*Henry Community Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!74SoiCnESMaSi9f-mZZu3TuQpthIBblOoUALh7lOXnMsH4PDJqAkjJJaOlLf5219UuzvTw$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat cooling time
The amount of time a cryostat takes to get to cutting temperature depends on the age of the machine, manufacturer, and air flow around the cryostat. We have 4 Avantiks the newer ones cool faster than the older ones and the one that is under a wall cabinet with about 2 feet from the top of the cryostat to the bottom of the cabinet takes about an hour longer to cool. The cryostats are kept at -27. The cooling time for the quicker one is 2 hours from room temperature (70 degrees Fahrenheit) and the slower one is 3 hours. I would suggest you plan for 4 hours and monitor the cooling time so you know for the next time you need to shutdown the cryostat. I have had Leicas and Thermo Fisher cryostats and they were different. Hope this helps. -Original Message- >From: histonet-requ...@lists.utsouthwestern.edu >Sent: Sep 5, 2019 1:00 PM >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 190, Issue 3 > >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. Re: CPT Code 88172 (Webster, Thomas S.) > 2. Re: CPT Code 88172 (Joe W. Walker, Jr.) > 3. Re: CPT Code 88172 (Victoria Baker) > 4. PPE for frozen sections (Moe, Barbi A) > 5. DAKO Artisan special stain kit validations (Moe, Barbi A) > 6. Cryostat to room temperature (Moe, Barbi A) > > >-- > >Message: 1 >Date: Wed, 4 Sep 2019 17:31:03 + >From: "Webster, Thomas S." >To: "'histonet@lists.utsouthwestern.edu'" > >Subject: Re: [Histonet] CPT Code 88172 >Message-ID: >Content-Type: text/plain; charset="us-ascii" > >You can only bill for 88172 if the pathologist looks at the FNA slide(s) >during the procedure. Otherwise do not attempt to bill for it. It is a >physician code. > > >CONFIDENTIALITY NOTICE: >This e-mail message, including all attachments, is for the sole use of the >intended recipient(s) and may contain confidential and privileged >information. You may NOT use, disclose, copy or disseminate this >information. If you are not the intended recipient, please contact the >sender by reply e-mail immediately. Please destroy all copies of the >original message and all attachments. Your cooperation is greatly >appreciated. >Columbus Regional Hospital >2400 East 17th Street >Columbus, Indiana 47201 > >-- > >Message: 2 >Date: Wed, 4 Sep 2019 18:07:26 + >From: "Joe W. Walker, Jr." >To: "Webster, Thomas S." >Cc: "histonet@lists.utsouthwestern.edu" > >Subject: Re: [Histonet] CPT Code 88172 >Message-ID: > > > >Content-Type: text/plain; charset="utf-8" > >Hi Thomas, > >What is your reference source for stating this is "a physician code"? In >reviewing our coding data, this code carries a facilities component in >addition to a professional component. I agree that a pathologist must perform >the review in order to bill for both, but I am aware of people in New England >who bill just the TC when a technologist provides the adequacy interpretation >and some payers cover this charge. > >Joe W. Walker, Jr. MS, SCT(ASCP) >Anatomical Pathology Manager >joewal...@rrmc.org, www.rrmc.org > >-Original Message- >From: Webster, Thomas S. via Histonet >Sent: Wednesday, September 4, 2019 1:31 PM >To: 'histonet@lists.utsouthwestern.edu' >Subject: Re: [Histonet] CPT Code 88172 > >[External Email] This email originated from outside of the organization. Think >before you click: Don?t click on links, open attachments or respond to >requests for sensitive information if the email looks suspicious or you don?t >recognize the sender. > > >You can only bill for 88172 if the pathologist looks at the FNA slide(s) >during the procedure. Otherwise do not attempt to bill for it. It is a >physician code. > > >CONFIDENTIALITY NOTICE: >This e-mail message, including all attachments, is for the sole use of the >intended recipient(s) and may contain confidential and privileged information. >You may NOT use, disclose, copy or disseminate this information. If you are >not the intended recipient, please contact the sender by reply e-mail >immediately. Please destroy all copies of the original message and all >attachments. Your cooperation is greatly appreciated. >Columbus Regional Hospital >2400 East 17th Street >Columbus, Indiana 47201 >___ >Histonet mailing list >Histonet@lists.utsouthwestern.edu
Re: [Histonet] cryostat decontamination
Our last discussion with TJC to clarify their definition of "regular interval" was to follow the manufacturers guidelines. I recommend determining what manufacturer suggests with the use of the UV decon and write that in your policy that addresses this standard. If you are following their guidelines, you should be in compliance. Aimee -Original Message- From: Nancy Schmitt via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, August 15, 2016 9:58 AM To: ' Subject: [Histonet] cryostat decontamination ANP.23410 states that cryostat is defrosted and wiped down at regular intervals. What if you have UV decontam? Is there a ruling on that? Thanks Nancy Schmitt MLT, HT(ASCP) Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat Chirp
Gary I think that might have to do with the fan motor inside the unit, it might need to be replaced. We had ours replaced a few years ago. Do you service/PM the cryostat yearly? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Martin, Gary via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, May 03, 2016 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Chirp My Leica CM 1850 cryostat is making a chirping sound and I can't figure out what is doing this. Has anyone experienced this sound, if what was the resolve. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat with UV disinfection
Our Mohs Lab is CAP accredited. Every seven days of use, cryostats are shut down. Once machine has been cleaned and dried we use Cavi-wipes(super Sani-Cloth from PDI Inc.) We then document disinfection date on QC Log. We have three cryostats, we rotate a cryostat a week for disinfecting purposes. We added this to our Equipment: Cryostats/Microtomes policy. Hope this helps. Karen Pfaff, HT (ASCP), BA Lead Histotechnician Skin Cancer Center Froedtert Health Phone: 414-805-6010 Fax: 414-805-5325 -Original Message- From: Terri Braud [mailto:tbr...@holyredeemer.com] Sent: Friday, March 11, 2016 12:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Cryostat with UV disinfection For those of you that are CAP accredited, and use a cryostat with UV disinfection, how do you get around the CAP requirement listed below? We were told the UV disinfection did not replace wiping down with a tuberculocidal disinfectant at room temp. Do you have an approved tuberculocidal disinfectant that will work at cryostat temps? Curious minds would like to know! ANP.23410 Cryostat DecontaminationPhase II There is a written procedure for the decontamination of the cryostat at defined intervals, and under defined circumstances, and decontamination records are evident. NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed surfaces with tuberculocidal disinfectant. The cryostat should be at room temperature during decontamination unless otherwise specified by the manufacturer. This should be done at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. Terri L. Braud, HT(ASCP) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat with UV disinfection
For those of you that are CAP accredited, and use a cryostat with UV disinfection, how do you get around the CAP requirement listed below? We were told the UV disinfection did not replace wiping down with a tuberculocidal disinfectant at room temp. Do you have an approved tuberculocidal disinfectant that will work at cryostat temps? Curious minds would like to know! ANP.23410 Cryostat DecontaminationPhase II There is a written procedure for the decontamination of the cryostat at defined intervals, and under defined circumstances, and decontamination records are evident. NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed surfaces with tuberculocidal disinfectant. The cryostat should be at room temperature during decontamination unless otherwise specified by the manufacturer. This should be done at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. Terri L. Braud, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat troublshooting. CM3050
Hello Patrick, We have this same cryostat except it's the CM 3050S. Ours also has this issue usually after it's been running for many months with a defrost. The typical cause of your problem is the cylinder on which the block holder is attached has ice crystals or is frozen. I feel the cause of this is because the glass was left open too long while cutting. This allowed moister from the room to condense on the cylinder and subsequently causes a stop go motion. Since I am in charge of all histology equipment maintenance, my solution is to turn off the cryostat, take the microtome out of the cavity and let it dry overnight. Then in the morning, I physically dry the microtome. Using cryostat oil, oil all the moving parts including the cylinder, then wipe off excess. Before putting the microtome back into the cavity, the cavity itself must also be thoroughly dried. The final cool down process usually takes about 1-2 hours. Feel free to email any specific questions. Hope this helps. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Wed, Jan 7, 2015 at 6:42 PM, Lewis, Patrick patrick.le...@seattlechildrens.org wrote: H everyone, We have been using this old CM3050 cryostat for our Frozen OCT blocks. I am cutting at 5 uM. My issue is that when I use the hand rotator to cut my sections it doesn't seem to advance with each rotation. It can take as many as 4 rotations to go from one section to another. What would cause this, and is there an easy solution to this problem? I can use the button to automatically move the chick holder all the forward and back from blinking to blinking, so I am wondering if ice crystals or debris can be ruled out as the issue. Any advice welcome. Thanks Patrick. ps: when I do get the blade to cut into the block, I have to wonder if the section I am getting is really 5 uM, or is it thicker. Sometimes you can tell if the section looks particularly thick, but other times it's hard to tell. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat design
Dear Kim, Yes the Starlet cryostat made by Bright Instrument Company, it is in a small bench level cabinet as there is no other way of achieving good results on the refrigeration. Apart from freezing microtones which were highly inefficient on retrieving good quality thin sections. Hope this assists you. Alan Bright BIC..UK Sent from my iPhone On 5 Mar 2014, at 16:55, Kolman, Kimberly D. kim.kol...@va.gov wrote: Hello all; Is anyone aware of a cryostat design in which the microtome cabinet would sit at bench level? In other words, it would function like any regular bench microtome but be housed in a cold cabinet like a cryostat? This would enable the operator to work in a desk position instead of uncomfortably being hunched over a cryostat cabinet. Thanks in advance, Kim Kimberly D. Kolman, HT (ASCP) VA Eastern Kansas Health Care System 4101 S 4th St Trfwy Leavenworth KS 66048 913-682-2000x 52537 Fax: 913-758-4193 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat
Tanya and Madeline, Jan Minshew of Leica sent a nicely detailed response to Decontaminating a cryostat in January of 2010. She apparently wrote it to put in a Leica How To manual, although I did not notice it in this form. Everything she states is well documented, although I also incorporate a known virucide and tuberculocidal product (following it's directions, naturally). http://lists.utsouthwestern.edu/pipermail/histonet/2010-January/048750.html If you have a UV cryostat, use Leica's Cryofect product or follow Tim Morken's previous (2000 histosearch)formaldehyde method, they should work for killing most bugs too(with the exception of eradicating some prions like CJD/BSE). If I am misunderstanding what you mean by Decon I apologize, as there is no reason why you cannot clean up tissue debris in a cold cryostat with 100% alcohol. Hugh Hawaii -- Date: Thu, 16 Jan 2014 10:43:09 -0800 (PST) From: Madeline Gi madelin...@yahoo.com Subject: Re: [Histonet] Cryostat To: Abbott, Tanya tanyaabb...@catholichealth.net, Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 1389897789.16602.yahoomail...@web121504.mail.ne1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 I use to decon my cryostat with 100% Alco this keeps it from freezing up and it clean it. best of luck. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com On Wednesday, January 15, 2014 2:52 PM, Abbott, Tanya tanyaabb...@catholichealth.net wrote: Need to decon my cryostat Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat
I use to decon my cryostat with 100% Alco this keeps it from freezing up and it clean it. best of luck. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com On Wednesday, January 15, 2014 2:52 PM, Abbott, Tanya tanyaabb...@catholichealth.net wrote: Need to decon my cryostat Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat service
Avantik is located in NJ and services cryostats in addition to microtomes, processors and stainers. www.avantik-us.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Ross Sent: Monday, October 28, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat service Hi everyone, I am interested in finding out if there is anyone in or close to Delaware that services and repairs cryostats. Any recommendations would be much appreciated. Thanks, Jean -- Jean Ross Delaware Biotechnology Institute, BioImaging Center University of Delaware 15 Innovation Way Suite 117 Newark, DE 19711 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat service
Bel Air Instruments and Dolbey Jamison Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni [trathbo...@somerset-healthcare.com] Sent: Monday, October 28, 2013 2:29 PM To: 'Jean Ross'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat service Avantik is located in NJ and services cryostats in addition to microtomes, processors and stainers. www.avantik-us.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Ross Sent: Monday, October 28, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat service Hi everyone, I am interested in finding out if there is anyone in or close to Delaware that services and repairs cryostats. Any recommendations would be much appreciated. Thanks, Jean -- Jean Ross Delaware Biotechnology Institute, BioImaging Center University of Delaware 15 Innovation Way Suite 117 Newark, DE 19711 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat cleaning
Ann, There are many tuberculocidal cleaners. Just search for it. If you are in a hospital the central supply will most likely have some in stock. We use PD-128 by Spartan Chemical http://www.spartanchemical.com/products/product/101604 Here are some references for cryostat cleaning: Cryostat decontamination references: College of American Pathologists, Commentary on certification questionnaire: 1) Decontaminate regularly with 70% alcohol. 2) Defrost, remove trimmings and decontaminate with a tuberculocidal disinfectant, preferably weekly for instruments used daily 3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline M29-A. Wayne, PA, NCCLS, 1997 NCCLS site: http://www.nccls.org/ Decontamination for HIV Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J histotechnol, V9, No.1, p.29 (March 1986) Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective against HIV). Department of Agriculture for Northern Ireland, Safe Working and the Prevention of Infection in Clinical Laboratories, Health Services Advisory Committee. Recommends formalin fuming for 24-48 hours at room temperature. Followed by ammonia for one hour (presumably as a neutralizer). Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill Livingstone, 1994. Recommends defrosting , washing with 70% alcohol, complete cleaning. (no references given). For those who like to read a lot: APIC Guideline for Selection and Use of Disinfectants, Association for Professionals in Infection Control (APIC), American Journal of Infection Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in use and the pros and cons of each. Tim Morken UCSF Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Friday, August 16, 2013 8:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat cleaning ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria listed above. thankis, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat cleaning
We wipe out the excess tisue( if there is any), wipe down the chamber with abs. Alcohol then spray the chamber with CaviCide, let it sit for @ 3-5 minutes and then wipe the chamber out again. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Friday, August 16, 2013 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat cleaning ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria listed above. thankis, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat Cleaning
Ann, Cryostat decontamination references: College of American Pathologists, Commentary on certification questionaire: 1) Decontaminate regularly with 70% alcohol. 2) Defrost, remove trimmings and decontaminate with a tuberculocidal disinfectant, preferably weekly for instruments used daily 3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline M29-A. Wayne, PA, NCCLS, 1997 http://www.cap.org/HTML/checklist_html/cklst08p.html NCCLS site: http://www.nccls.org/ Decontamination for HIV Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J histotechnol, V9, No.1, p.29 (March 1986) Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective against HIV). Department of Agriculture for Northern Ireland, Safe Working and the Prevention of Infection in Clinical Laboratories, Health Services Advisory Committee. Recommends formalin fuming for 24-48 hours at room temperature. Followed by ammonia for one hour (presumably as a neutralizer). Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill Livingstone, 1994. Recommends defrosting , washing with 70% alcohol, complete cleaning. (no references given). For those who like to read a lot: APIC Guideline for Selection and Use of Disinfectants, Association for Professionals in Infection Control (APIC), American Journal of Infection Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in use and the pros and cons of each. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Tuesday, August 06, 2013 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Cleaning Can someone tell me the CAP approved chemical that can be used to clean the cryostat? thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat
I would not vacuum the trimmings out of cryostat, if it were infected It would make a good job of contaminating a laboratory etc. Terrified Alan Bright Sent from my iPhone Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw this in one lab!?thanksIrena ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat
Happy Wednesday Everyone There are vacumes specifically made for cryostats that are hepa filtered, etc. We have one of those. The company is called MARMED. We use a shop vac for the paraffin microtomes, that works nicely. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alan Bright [abri...@brightinstruments.com] Sent: Wednesday, February 06, 2013 9:31 AM To: IRENA SREBOTNIK KIRBIS Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryostat I would not vacuum the trimmings out of cryostat, if it were infected It would make a good job of contaminating a laboratory etc. Terrified Alan Bright Sent from my iPhone Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw this in one lab!?thanksIrena ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat
Hi Irena! If you go to IMEB Inc.'s website, and type vacuum into the search, it will take you to what we use. They sell a vacuum cleaner and accessories. We currently use the filtered hoses with an old canister vacuum that's been around longer than I have. When the filter gets full, we discard it in the biohazard trash and replace it with a new one. Hope this helps! Laura From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS [irena.kir...@hotmail.com] Sent: Wednesday, February 06, 2013 8:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw this in one lab!?thanksIrena ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat Maintenance and Repair - CA
Hi Eric, Mikron Instruments services most of our instruments, including Micron brand. I don't have any Leica equipment now, but they have serviced my Leica microtome in the past. They are very good! The service manager's name is Steve Pike. Mikron is in Vista, CA and their phone number is (800) 377-5395. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez Sent: Wednesday, May 04, 2011 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Maintenance and Repair - CA Hi, Can somebody please recommend to me a company that does cryostat maintenance and repair? I am looking for a company that is familiar with both Micron and Leica models in the Irvine, CA area. Thanks, Eric ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat repair--thanks!
The compressor fan was loose, so when the Tech One guy fixed it, instead of making a horrible noise when it turns off, it just rattles. Oh well, time for a new motor. Which leads me to another question, is there any reason I should call Lecia to replace this motor? The guy from Tech One Biomedical seemed to know his stuff, but my boss seems to think we should call the people who made the cryostat. Personally, I think that would be spending a lot more money for the same thing, but maybe I'm missing something. Any suggestions? By the way, the cryostat is from 2008 and isn't under warranty. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Apr 1, 2011 at 3:15 PM, Victor Tobias vic...@pathology.washington.edu wrote: So what was the problem? Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 4/1/2011 12:09 PM, Emily Sours wrote: Thanks to all who answered my email, I ended up calling Tech One Biomedical and they came right out. I recommend them to anyone who needs histology equipment repaired!! Emily ps. this is not an april fool's joke. pps. really! It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat repair--thanks!
The Tech One guy would do the same thing for much cheaper. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, April 05, 2011 10:11 To: Victor Tobias; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryostat repair--thanks! The compressor fan was loose, so when the Tech One guy fixed it, instead of making a horrible noise when it turns off, it just rattles. Oh well, time for a new motor. Which leads me to another question, is there any reason I should call Lecia to replace this motor? The guy from Tech One Biomedical seemed to know his stuff, but my boss seems to think we should call the people who made the cryostat. Personally, I think that would be spending a lot more money for the same thing, but maybe I'm missing something. Any suggestions? By the way, the cryostat is from 2008 and isn't under warranty. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Apr 1, 2011 at 3:15 PM, Victor Tobias vic...@pathology.washington.edu wrote: So what was the problem? Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 4/1/2011 12:09 PM, Emily Sours wrote: Thanks to all who answered my email, I ended up calling Tech One Biomedical and they came right out. I recommend them to anyone who needs histology equipment repaired!! Emily ps. this is not an april fool's joke. pps. really! It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat repair
Emily You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and do most of our repairs. Ray does an excellent job at a good price. It sounds like the bearings in your fan or maybe the compressor. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, March 31, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat repair Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refrigeration that's broken, it sounds like something mechanical. Any suggestions? Has anyone ever tried to open the back of one to see if it's fixable? I'm no mechanic, but I think I can spot a worn belt or loose screw. I'm just not sure I want to open it up myself. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat repair
We use them too- Illinois office. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bouchal, Rena L Sent: Thursday, March 31, 2011 1:38 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat repair We are using Tech One Biomedical... service reps in Pghfor almost all of our routine instrument service really good and half the price of the vendors! Their number is 866-497-3033 or www.techoneweb.com Please note that my email address as of Jan 3, 2011 is bouch...@wvuhealthcare.com . Please make the appropriate changes in your address book. Rena Bouchal, M.S. Anatomic Pathology Manager West Virginia University Hospitals 304-293-7765 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DANIEL HEWITT Sent: Thursday, March 31, 2011 2:15 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat repair Emily You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and do most of our repairs. Ray does an excellent job at a good price. It sounds like the bearings in your fan or maybe the compressor. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, March 31, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat repair Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refrigeration that's broken, it sounds like something mechanical. Any suggestions? Has anyone ever tried to open the back of one to see if it's fixable? I'm no mechanic, but I think I can spot a worn belt or loose screw. I'm just not sure I want to open it up myself. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
Hi Diane, We have been happy with the Leica cryostats over the years. We have an 1800 that is about 15 years old, an 1850 that is about 5 years old with the UV light for decontamination. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Gladney, Diane C Ms CIV USA MEDCOM MACH diane.glad...@us.army.mil To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wed, March 30, 2011 7:50:23 AM Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) Classification: UNCLASSIFIED Caveats: NONE Dear Histonetters, Our 11 year old cryostat is beginning to experience some unusual problems. Our Medical Maintenance says that they won't put a lot of money into fixing it because of the age of the unit. Therefore, I am asking for opinions, pros, cons of different brands of cryostats. I am interested in a cryostat that has the UV light or other source of easy decontamination. We don't do a lot of frozen sections but enough for us to have to have a good cryostat that is easy to operate and clean. Any comments or suggestions would be greatly appreciated. Thanks, Diane Gladney Diane C. Gladney, HT (ASCP) Histology Supervisor Department of Pathology Moncrief Army Community Hospital 4500 Stuart Street FT. Jackson, SC 29207 Phone: 803-751-2530 Fax: 803-751-7829 Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
Hi Diane, We just purchased a cryostat from Sakura, we love it. Great features. Date: Wed, 30 Mar 2011 06:38:41 -0700 From: dch...@yahoo.com To: diane.glad...@us.army.mil; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) CC: Hi Diane, We have been happy with the Leica cryostats over the years. We have an 1800 that is about 15 years old, an 1850 that is about 5 years old with the UV light for decontamination. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Gladney, Diane C Ms CIV USA MEDCOM MACH diane.glad...@us.army.mil To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wed, March 30, 2011 7:50:23 AM Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) Classification: UNCLASSIFIED Caveats: NONE Dear Histonetters, Our 11 year old cryostat is beginning to experience some unusual problems. Our Medical Maintenance says that they won't put a lot of money into fixing it because of the age of the unit. Therefore, I am asking for opinions, pros, cons of different brands of cryostats. I am interested in a cryostat that has the UV light or other source of easy decontamination. We don't do a lot of frozen sections but enough for us to have to have a good cryostat that is easy to operate and clean. Any comments or suggestions would be greatly appreciated. Thanks, Diane Gladney Diane C. Gladney, HT (ASCP) Histology Supervisor Department of Pathology Moncrief Army Community Hospital 4500 Stuart Street FT. Jackson, SC 29207 Phone: 803-751-2530 Fax: 803-751-7829 Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat help
That depends on what staining you need to use. If an IHC, just fix the sections in acetone, air dry and proceed without HIER If HE fix quickly with formalin 10%, wash → hematoxylin for 30 secs without differentiation → eosin 15 sec→ dehydrate and mount. Other HC procedures all will require fixation and using the specific protocol. Always remember that it is a frozen section and therefore more delicate and less adhered than a paraffin section. René J. --- On Tue, 1/11/11, Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca wrote: From: Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca Subject: [Histonet] Cryostat help To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 11, 2011, 10:28 AM Hello Histonetters, I am new to the histology world and I was hoping to get some feedback about staining after cutting sections in the cryostat. Does anyone have a protocol they use for staining after sectioning tissue in a cryostat? I have never done it before so I thought I would come to the experts. Thanks for your help! Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat help
We have a similar frozen section protocol but we fix the slide in 95% alcohol for ~1minute and then stain with Gill's Hematoxylin for 1 minute, rinse, blue and then alcohol, foloowed by Eosin for ~ 15secs.- dehydrate and mount. Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 11, 2011 10:29 AM To: histonet@lists.utsouthwestern.edu; KimberlyBlundon Subject: Re: [Histonet] Cryostat help That depends on what staining you need to use. If an IHC, just fix the sections in acetone, air dry and proceed without HIER If HE fix quickly with formalin 10%, wash → hematoxylin for 30 secs without differentiation → eosin 15 sec→ dehydrate and mount. Other HC procedures all will require fixation and using the specific protocol. Always remember that it is a frozen section and therefore more delicate and less adhered than a paraffin section. René J. --- On Tue, 1/11/11, Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca wrote: From: Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca Subject: [Histonet] Cryostat help To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 11, 2011, 10:28 AM Hello Histonetters, I am new to the histology world and I was hoping to get some feedback about staining after cutting sections in the cryostat. Does anyone have a protocol they use for staining after sectioning tissue in a cryostat? I have never done it before so I thought I would come to the experts. Thanks for your help! Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat Question Please
We have this model as well and like it. We got the vacuum attachment. It's not perfect, but does facilitate cleaning up OCT and tissue scraps. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 24, 2010 4:14 PM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cryostat Question Please Very consistant, easy to use. 2 drawbacks 1. We got the decontamination add-on trying to automate the CAP requirement, but now it seems Thermo says it is not an adequate process. I believe they are doing more intense studies, but for the time being, I have to decontaminate it by hand. 2. OCT and tissue scraps build up under collection device and cause the mechanism to clunk unless buildup is removed. These two things add a good chunk of time to my day. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, November 24, 2010 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Question Please Hello all Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat? If so, what is your opinion/pros and cons? Thanks so much in advance, Paula Lab Manager BP Medical Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat Question Please
Very consistant, easy to use. 2 drawbacks 1. We got the decontamination add-on trying to automate the CAP requirement, but now it seems Thermo says it is not an adequate process. I believe they are doing more intense studies, but for the time being, I have to decontaminate it by hand. 2. OCT and tissue scraps build up under collection device and cause the mechanism to clunk unless buildup is removed. These two things add a good chunk of time to my day. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, November 24, 2010 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Question Please Hello all Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat? If so, what is your opinion/pros and cons? Thanks so much in advance, Paula Lab Manager BP Medical Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat question
Dear Susan, Our Starlet 2212 Bench Top Cryostat has an option for orientation. There are many used for Mohs, as holding a low temperature is not a problem. I can email you some user references if. You want. Regards: Alan Bright Bright Instrument Co. Ltd. England Sent from my BlackBerry® wireless device -Original Message- From: histo...@aol.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Fri, 29 Oct 2010 22:10:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat question Does anyone have 'hands on' experience performing Mohs using a countertop cryostat? Can the specimen head be manipulated to acccomodate a badly embedded block? Does the machine stay cold enough to perform Mohs? Any advice would be appreciated. My e-mail is histo...@aol.com. Thanks, Susan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS -- Teach SpamSniper if this mail (ID 01DoOb8La) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01DoOb8Lam=99b5dbb60f3dc=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01DoOb8Lam=99b5dbb60f3dc=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01DoOb8Lam=99b5dbb60f3dc=f -- END-ANTISPAM-VOTING-LINKS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat
Jeff, We have both the Leica CM1850 and the CM1850 UV. These are not top of the line models, but they work very well for us. You can find out more information on the unit at the following URL: http://www.leica-microsystems.com/products/histology-systems/sectioning/cryo stats/details/product/leica-cm1850-uv/ Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Thompson Sent: Friday, August 20, 2010 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat Hello, I am writing to ask about what the community views are on the best -or worst - cryostats. We may have the opportunity to purchase one for the first time in many years and we would like to get a good, solid, reliable model. We're not really looking for one (and can't really afford one) with all the bells and whistles, but would like to know what folks think about the ones to look for as well as the ones to steer clear of. All input is appreciated. Thanks, Jeff Thompson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3071 - Release Date: 08/15/10 18:35:00 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat
Jeff, please note that the URL posted below wrapped into two lines. Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Sunday, August 22, 2010 12:11 PM To: 'Jeffrey Thompson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat Jeff, We have both the Leica CM1850 and the CM1850 UV. These are not top of the line models, but they work very well for us. You can find out more information on the unit at the following URL: http://www.leica-microsystems.com/products/histology-systems/sectioning/cryo stats/details/product/leica-cm1850-uv/ Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Thompson Sent: Friday, August 20, 2010 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat Hello, I am writing to ask about what the community views are on the best -or worst - cryostats. We may have the opportunity to purchase one for the first time in many years and we would like to get a good, solid, reliable model. We're not really looking for one (and can't really afford one) with all the bells and whistles, but would like to know what folks think about the ones to look for as well as the ones to steer clear of. All input is appreciated. Thanks, Jeff Thompson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3071 - Release Date: 08/15/10 18:35:00 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.441 / Virus Database: 271.1.1/3087 - Release Date: 08/22/10 06:35:00 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat cutting problems
It may help to let your block equilibrate for about 20 minutes in the cryostat. I've found blocks do not section well if you start sectioning right away, especially snap frozen ones. Also, do you embed in OCT/sucrose? A 1:1 OCT: 30% sucrose solution is much softer than just OCT (or whatever embedding medium you are using). What do you mean by the tissue is condensed? It's folding up? I've found sectioning fresh unfixed tissue on a cryostat is impossible (we tried chick embryo trunks, which may be harder to section fresh than other tissue)--it doesn't section but get smushed to a pulp. You need to use a sledge microtome, unfortunately. What thickness are you sectioning at? I've found sectioning above sixty microns doesn't work as well on a cryostat, but again this is on fixed chick embryo tissue. If your cryostat is old, I would suggest you look for a new anti-roll plate made of glass. They work so much better. As long as your cryostat isn't older than about ten years, you should be able to find one that fits your knife holder very easily. That said, I've used a Rei-something cryostat that was 15 years old and it sucked. The anti-roll plate holder was too loose to do anything useful and the adjustment of the blade holder was impossible. Can you use a newer cryostat in your department from another lab? Or just go back to sledge microtome sectioning--if you know how to do it, it's the same principle anyway. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Fri, Aug 13, 2010 at 4:53 PM, Entwistle, Laura lentwis...@ucsd.eduwrote: I am new to using a cryostat and am having some issues with my tissue. 1.Every slice seems to shatter and fragment. When I have used a microtome it was because the tissue was too cold. 2. The tissue itself was flash frozen and so was not fixed and the tissue did not go through the perfusion process. 3. Each slice is condensed and doesn't lay flat. 4. The cryostat is old. Any tips would be very helpful. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat vs decal sections of bone
Most definitely there are differences. Just consider that the bone will not be subjected to any chemicals because neither fixation nor demineralization will be required., René J. --- On Mon, 5/10/10, louise renton louise.ren...@gmail.com wrote: From: louise renton louise.ren...@gmail.com Subject: [Histonet] Cryostat vs decal sections of bone To: Histonet@lists.utsouthwestern.edu Date: Monday, May 10, 2010, 4:19 AM Hi all, last year sometime I was asked to budget for some equipment for our unit. Not expecting to get anything i aimed high, and requested a cryostat and cryoJane system. Lo and behold, the planetary influences were just right, and my request was approved. Now, I've got cold feet (pardon the pun) and I'm wondering if there *are* any distinct advantages of cryosections of bone over demineralised wax embedded samples in regards to immuno and in-situ ?. -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat help please!
Dear Mohamed, We manufacture cryostats but not the one you have the problem with, however I think what you can hear is the cooling fan working but not the refrigeration compressor. You will need to locate the internal fuses to see which ones need replacing. If the compressor is running then it would seem to have lost its refrigeration gas. Unfortunately I am leaving my office now and Histonet does not seem to like my Blackberry, but if you would like to talk further on this problem you can catch me on Skype dazzle0 later today or let me have your phone number and I will call you. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abri...@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: 20 March 2010 13:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat help please! hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat help please!
Sounds like it may be a fuse... in my non-expert opinion!! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of mohamed abd el razik Sent: Sat 3/20/2010 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat help please! hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat help please!
our cryostat is Minotome Plus Microtome Cryostat Cat. No. 2563-- For 120 VAC, 60Hz --- On Sat, 3/20/10, Cheryl Cornett-Early cornettear...@bellsouth.net wrote: From: Cheryl Cornett-Early cornettear...@bellsouth.net Subject: Re: [Histonet] cryostat help please! To: mohamed abd el razik k8...@yahoo.com Date: Saturday, March 20, 2010, 10:59 PM Hi Mohamed, I AM a Services guy and this problem sounds like a loss of refrigerant I come across this problem on older Cryostat's after they have been turned off regularly, What model of cryostat is it?. IT sounds like a CM1800 Riechet Jung or (Leica). Best regards Brian. My territory is the south east, GA, AL, TN, SC, NC, --- On Sat, 3/20/10, mohamed abd el razik k8...@yahoo.com wrote: From: mohamed abd el razik k8...@yahoo.com Subject: [Histonet] cryostat help please! To: histonet@lists.utsouthwestern.edu Date: Saturday, March 20, 2010, 9:30 AM hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? Histonet@lists.utsouthwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat help please!
I agree with Joyce, it sounds like the fuse for the refrigeration. I had that problem a few times. Robyn Vazquez Date: Sun, 21 Mar 2010 08:28:36 -0400 From: jwe...@sjha.org To: k8...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat help please! CC: Sounds like it may be a fuse... in my non-expert opinion!! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of mohamed abd el razik Sent: Sat 3/20/2010 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat help please! hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat help please!
thanks for your replay ray yes i set the temp. to -20 degrees but it is 20 for the last week. --- On Sat, 3/20/10, Mark Ray darkd...@comcast.net wrote: From: Mark Ray darkd...@comcast.net Subject: Re: [Histonet] cryostat help please! To: mohamed abd el razik k8...@yahoo.com Date: Saturday, March 20, 2010, 5:03 PM Are you sure the temperature control is set properly? This maybe a serious problem, but it is possible that the people who do the ordinary repairs on refrigeration equipment for the university can repair it. You should ask them if they can help you. mohamed abd el razik wrote: hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat better than others?
We currently have a Leica and a Jung. They are serviced twice a year. Prior to these, we had two Tissue Teks that were donated to us. My best guess is that they were about 20 years old when they were replaced. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Fairbairn, Patricia Sent: Thursday, March 04, 2010 2:59 PM To: Histonet Subject: [Histonet] Cryostat better than others? Hi All, Are there any cryostat makes that seem to be more reliable than others? We have not been having much luck with our particular Microms after moving them cross-country, getting recalled, catching on fire, corroding, etc. While one machine serves as an excellent calendar stand (with padded resting pads and everything!) and our other one just went down at the ripe age of 2, I would rather purchase machines that work without costing us so much in repairs. ...or are we just jinxed? Also, how long do cryostats tend to last? What do you think of service contracts? I keep hearing that they are pretty much worthless and that the cryostat should be fine if it's served once a year. Thank you! PJ Fairbairn Staff Research Associate Department of Neurological Surgery Brain and Spinal Injury Center University of California at San Francisco fairbai...@neurosurg.ucsf.edu mailto:fairbai...@neurosurg.ucsf.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat decon- what a pain in the butt
Jeff, Do they give any references for the effectiveness of their proposed method? Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Sunday, February 21, 2010 12:20 PM To: o.isaa...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat decon- what a pain in the butt CAP is moving to more rigorous cryostat decontamnation methods - mandating a weekly shutdown and wet chemical disinfection with a tuberculocidal agent for machines used regularly. Now, if a lab is doing 1-3 frozens a week, is that used regularly? We must lobby the CAP for more sensible and practical guidlines. The old wipe down with 70% ETOH without bringing the machine will become non-compliant and useful only as an interim measure. By the way, the UV lamps do not satisfy the CAP standard, I believe. Our system has gone to a commercially available tuberculocidal, virucidal, and broad spectrum bacteriocidal moistened wipes the name of which I will post tomorrow when I get to the job. Here is a skeletonized basic procedure form what CAP will require: 1: Remove all utensils and brush out and collect section debris disposing of this according to regulated medical waste protocol (red bag). 2. Bring the instrument to room temperature. 3: Wipe all working surfaces with the tuberculocidal wipes, visibly moistening all surfaces. Surface must remain wet for 2 minutes. Use multiple wipes as needed. Instruments can be similarly disinfected. 4: Carefully dry all surfaces with gauze. Dispose of all wipes and gauze as biohazardous. 5: Dry and lubricate the microtome as per manufacturer's instructions. 6. Turn on crystat and bring to working temperature. 7. Document procedure on your maintenance log. 8. Look forward to doing it again next week. Look, this is a necessary procedure, but weekly??? Perhaps some workload- based formula or an alert system- like pathologists alert the lab when a case with granulomas or caseating necrosis is sectioned. Every lab will have to bring a tech in on weekends or at night, to do this, or have two cryostats to compensate for the fully one working day most machines will have to be down to be cleaned in this manner. Thoughts? Jeff Silverman ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat decon- what a pain in the butt
Jeff, Can you give the reference for the CAP adjustment on decon for crystats please? We have two working cryostats and average 6 to 10 cases (not specimens) a day. This would effectively shut us down for frozens on some days. We have third one that is currently in for repair. Thanks, Pam Marcum UAMS Anatomic Patholgoy Manager - Original Message - From: Tim Morken timothy.mor...@ucsfmedctr.org To: Jeffrey Silverman pathmas...@yahoo.com, o isaac24 o.isaa...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Sent: Monday, February 22, 2010 10:23:17 AM GMT -06:00 US/Canada Central Subject: RE: [Histonet] Cryostat decon- what a pain in the butt Jeff, Do they give any references for the effectiveness of their proposed method? Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Sunday, February 21, 2010 12:20 PM To: o.isaa...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat decon- what a pain in the butt CAP is moving to more rigorous cryostat decontamnation methods - mandating a weekly shutdown and wet chemical disinfection with a tuberculocidal agent for machines used regularly. Now, if a lab is doing 1-3 frozens a week, is that used regularly? We must lobby the CAP for more sensible and practical guidlines. The old wipe down with 70% ETOH without bringing the machine will become non-compliant and useful only as an interim measure. By the way, the UV lamps do not satisfy the CAP standard, I believe. Our system has gone to a commercially available tuberculocidal, virucidal, and broad spectrum bacteriocidal moistened wipes the name of which I will post tomorrow when I get to the job. Here is a skeletonized basic procedure form what CAP will require: 1: Remove all utensils and brush out and collect section debris disposing of this according to regulated medical waste protocol (red bag). 2. Bring the instrument to room temperature. 3: Wipe all working surfaces with the tuberculocidal wipes, visibly moistening all surfaces. Surface must remain wet for 2 minutes. Use multiple wipes as needed. Instruments can be similarly disinfected. 4: Carefully dry all surfaces with gauze. Dispose of all wipes and gauze as biohazardous. 5: Dry and lubricate the microtome as per manufacturer's instructions. 6. Turn on crystat and bring to working temperature. 7. Document procedure on your maintenance log. 8. Look forward to doing it again next week. Look, this is a necessary procedure, but weekly??? Perhaps some workload- based formula or an alert system- like pathologists alert the lab when a case with granulomas or caseating necrosis is sectioned. Every lab will have to bring a tech in on weekends or at night, to do this, or have two cryostats to compensate for the fully one working day most machines will have to be down to be cleaned in this manner. Thoughts? Jeff Silverman ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat Poll
We have a LEICA CRYOSTAT... its EXCELLENT! Yes- we have a contract and NO never had issues... Maria Katleba HT(ASCP) MS Queen of the Valley Medical Center Napa CA 94558 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, December 01, 2009 2:22 PM To: Merced M Leiker Cc: Histonet Subject: Re: [Histonet] Cryostat Poll yes and yes Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello P Please consider the environment before printing this email. On Dec 1, 2009, at 12:12 PM, Merced M Leiker wrote: For Researchers Only who have cryostats (1-2 hrs/wk ave usage): 1. Is your cryostat under service contract? 2. Have you had any problems with your cryostat that would justify having a service contract? Thank you! Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat decontamination...
From what I understand if you require that an individual wear a N95 respirator when they are sectioning frozen sections then you are also required to have that respirator fit tested yearly. We are a research lab that does quite a bit of work on TB samples. We do not section TB infected samples on a cryostat. All samples that we receive have been fixed in 10% NBF for several days and then transferred to 70% alcohol. When I was in a clinical lab, we would not section frozen sections of lung samples if they wanted to rule out TB, the pathologist would recommend to process to paraffin first. In fact since the samples are generated in a biosafety level 2 or 3 facility, they can not leave that facility unless the samples have been fixed and rendered non infectious. Frozen sections would need to be prepared within that biosafety facility with all of the appropriate PPE's in place. The University that we work with has tested these samples via culture after their fixation and alcohol procedure. We do however offer the N95 respirator to the techs and they can wear it if they want to, when they are grossing, embedding or sectioning these samples. But since it is voluntary we do not have to fit test, we have a specific procedure that covers this. This protocol has been developed with the help of our local OSHA rep. The other thing is we use a special vacumme with a hepa filter to vacume up the paraffin trimmings. OSHA has a program for small businesses and will work with them to make sure that they are within compliance, they have been very helpful to us here. Just go to the OSHA website. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, November 16, 2009 12:17 PM To: histo...@pathology.swmed.edu Subject: [Histonet] Cryostat decontamination... Hello all, We have always used absolute alcohol to decontaminate our cryostat and wondered what others use. This has been brought up by an article in the September issue of CAP Today that talks about biosafety when doing frozens (specifically talks about TB). As a related question, how many institutions require the wearing on an N95 respirator when doing frozens? The article states that diluted alcohol is more effective because the presence of water causes proteins to denature more quickly. I just did the decontamination of our cryostat last weekend and used 70% alcohol as the article suggested. The alcohol evaporated and I was left with beads/drops of water that I had to then dry by hand. I guess the alternative would be is just to go over it again with aboslute after usiing 70%. The article goes on to recommend that personnel should wear N95 masks that are fit tested on a yearly basis. Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat adapter
We have the adapter here at IMEB, Inc. Please go to www.imebinc.com for our contact info and ask for Brad. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat safety question
Hello, I had previously sent this but only to the person who asked the question, I think it this works beautifully so decided to resend to the list. we have a great way to remove the specimens for the chucks. We have a 500cc plastic container with a lid that we have cut an X into the center. We put warm water in the container and then just put the chuck stem into the X. Within 10 seconds the block can be removed. Still frozen. We just keep the plastic container at the bench and keep reusing the same one. The water level does need to come all the way up to the lid, But the chuck never gets wet. We do the sealing of the tissue with a tiny amount of OCT to the surface of the still frozen block, while it is still in the cryostat as well. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, January 07, 2009 7:38 PM To: Histonet Cc: mari.ann.mailh...@leica-microsystems.com Subject: RE: [Histonet] Cryostat safety question Andrea: I work in a Mohs clinic where all we cut is frozen skin sections. Needless to say, we don't have 50 chucks laying around... In the morning before clinic starts we put a layer of freezing medium on chucks and put them in the cryostat to freeze. When we get specimens, we add another drop or so to the already frozen 'button' and immediately embed the tissue in it. We usually add another small drop on top after it has begun to freeze, to cover the specimen completely. Cut as normal when frozen. After done cutting all you have to do is use a forceps or other blunt object and pop the bit with the specimen in it away from the 'button' and return the chuck to the cryostat and it can be reused the rest of the day. The specimen is therefore still frozen for storage, and it has a quicker TAT. Plus you won't need nearly so many chucks, as they can be recycled almost as soon as you are done cutting. I usually keep 6-8 'buttons' in my cryostat, and our clinic can process up to 50 separate specimens a day. A word of caution. If your work area is humid sometimes a thin layer of frost can form on the surface of the 'button' and when you attempt to take sections the bit with the tissue will pop off the 'button'. All you need to do is add another drop of medium to the button and 'glue' the two back together. If you are going a while between cutting sessions, I usually store my 'buttons' upside(mountant side) down on one of the cryostat surfaces. It doesn't seem to develop the frost layer. Useful if you have tiny specimens. Hope my verbose explanation is helpful. Feel free to e-mail if you have any questions or are confused about my explanation. Claire Ingles Madison WI From: histonet-boun...@lists.utsouthwestern.edu on behalf of Andrea Hooper Sent: Wed 1/7/2009 5:40 PM To: Histonet Cc: mari.ann.mailh...@leica-microsystems.com Subject: [Histonet] Cryostat safety question The discussion on microtome safety begs me to ask a cryostat question We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat safety question
Hi Andrea, It's great to hear that you are happy with your cryostat and, hopefully, I'll be able to help you with an answer to your question. Leica sells a small device called a Thermal Block that is designed to help you remove the specimen from the chuck without causing it to melt. The catalog number is 14039818542. It's a small, mobile device that can be used in any of our cryostats that use chucks with stems in the back (we have a different one for the new CM1950 flat backed chucks). If you have questions or would like additional information, please feel free to contact our Technical Applications Center (1-800-248-0123 option 1 then option 2). Best wishes, Jan Minshew, HT/HTL(ASCP) Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 Toll Free 847.405.7051 Direct 847.405.6560 Fax www.leica-microsystems.com Click Here for this month's special offers! Andrea Hooper anh2...@med.corn ell.edu To Sent by: Histonet histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu mari.ann.mailh...@leica-microsystem s.com Subject 01/07/2009 05:40 [Histonet] Cryostat safety question PM The discussion on microtome safety begs me to ask a cryostat question We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat safety question
Andrea: I work in a Mohs clinic where all we cut is frozen skin sections. Needless to say, we don't have 50 chucks laying around... In the morning before clinic starts we put a layer of freezing medium on chucks and put them in the cryostat to freeze. When we get specimens, we add another drop or so to the already frozen 'button' and immediately embed the tissue in it. We usually add another small drop on top after it has begun to freeze, to cover the specimen completely. Cut as normal when frozen. After done cutting all you have to do is use a forceps or other blunt object and pop the bit with the specimen in it away from the 'button' and return the chuck to the cryostat and it can be reused the rest of the day. The specimen is therefore still frozen for storage, and it has a quicker TAT. Plus you won't need nearly so many chucks, as they can be recycled almost as soon as you are done cutting. I usually keep 6-8 'buttons' in my cryostat, and our clinic can process up to 50 separate specimens a day. A word of caution. If your work area is humid sometimes a thin layer of frost can form on the surface of the 'button' and when you attempt to take sections the bit with the tissue will pop off the 'button'. All you need to do is add another drop of medium to the button and 'glue' the two back together. If you are going a while between cutting sessions, I usually store my 'buttons' upside(mountant side) down on one of the cryostat surfaces. It doesn't seem to develop the frost layer. Useful if you have tiny specimens. Hope my verbose explanation is helpful. Feel free to e-mail if you have any questions or are confused about my explanation. Claire Ingles Madison WI From: histonet-boun...@lists.utsouthwestern.edu on behalf of Andrea Hooper Sent: Wed 1/7/2009 5:40 PM To: Histonet Cc: mari.ann.mailh...@leica-microsystems.com Subject: [Histonet] Cryostat safety question The discussion on microtome safety begs me to ask a cryostat question We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat safety question
I have the same cyostat, but it doesn't matter which one you use for removing tissue from the chuck is a separate issue. Others may beg to differ from my technic, but I remove the block from the cryostat and set it on a paper towel for a couple of mintes. I do not thaw tissue, just soften it. I take a scapel or small weigh spatula and gently separate it off the chuck. With a thin layer of OCT in the plastic mold, I return the block back to its original labeled mold, wrap with aluminum foil, place in a ziploc, and store it back to the -80C. This is quick and limits exposure to higher temps. I have used blocks multiple times, especially for cross reactivity studies and never had a problem. I am very careful and need reusing the block for IHC receptor studies. Good luck. This is pretty easy and you can move on to the next block at the same time. Beth Millerman/SWC Senior Research Associate Stiefel Laboratories, Inc Andrea Hooper anh2...@med.cornell.edu Sent by: histonet-boun...@lists.utsouthwestern.edu Wed 07 Jan 2009 03:40 PM --- To Histonet histonet @lists.ut southwest ern.edu cc mari.ann. mailhiot@ leica-mic rosystems .com Subject [Histonet ] Cryostat safety question --- The discussion on microtome safety begs me to ask a cryostat question We have a Leica CM3050 cryostat and love it! How are people (and perhaps only those in research do this) removing their tissue from the chucks for future use? We often just section a few slides worth then put the block at -80 deg C for future studies. Needless to say, it's the most dangerous part of our day. So what are your suggestions for removing tissue from a chuck (and melting it isn't really a viable option)? Thanks in advance, Andrea -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat SOP
As part of the general SOP, under sectioning. René J. --- On Tue, 10/28/08, Michele Wich [EMAIL PROTECTED] wrote: From: Michele Wich [EMAIL PROTECTED] Subject: [Histonet] cryostat SOP To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 28, 2008, 3:46 PM An SOP question for those out there who are forced to write these tedious things: Do you maintain a separate cryostat SOP, or is it something that is typically included in a general microtome SOP? Any help with this would be greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat blues
Those who are full of hot air can also blow gently and will generate enough humidity to reduce the static. J:) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Emily Sours Sent: Wednesday, October 15, 2008 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryostat blues Unfortunately, this sounds like static electricity. Leica suggests spraying anti-static spray in the chamber (!). You could also ground the metal in the chamber using metal wire touching something metal outside the chamber (the Russian post-doc solution, very clever). We keep 100% EtOH in a small container (about 100 ml) in the back of the chamber to reduce static, which I learned from a long ago histonet post, so I have no idea why it works. Otherwise, buy an anti-static brush, which I hear exists, but have never seen. Emily -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat blues
Good going Only us OLD TECH know this. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Weems, Joyce Sent: Wednesday, October 15, 2008 9:29 AM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat blues Those who are full of hot air can also blow gently and will generate enough humidity to reduce the static. J:) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Emily Sours Sent: Wednesday, October 15, 2008 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryostat blues Unfortunately, this sounds like static electricity. Leica suggests spraying anti-static spray in the chamber (!). You could also ground the metal in the chamber using metal wire touching something metal outside the chamber (the Russian post-doc solution, very clever). We keep 100% EtOH in a small container (about 100 ml) in the back of the chamber to reduce static, which I learned from a long ago histonet post, so I have no idea why it works. Otherwise, buy an anti-static brush, which I hear exists, but have never seen. Emily -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
