Re: [Histonet] Cryostat anti-roll plate

[Bob Richmond via Histonet]

>
>
> My experience with anti-roll plates is mostly with the old International
> cryostats of days gone by. Pathologists usually disdained anti-roll plates
> and used an artist's brush to keep the section from rolling, but more
> proficient users depended on them. I learned to use them in a research
> histology lab. We sat at the side of the cryostat by the crank, rather than
> in front of it, and used an anti-roll plate. It was easy to cut fifty
> slides with it, which I required for my research fifty years ago.


Bob Richmond
retired samurai pathologist
Maryville TN

>
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Re: [Histonet] Cryostat anti-roll plate

[Keyser, Geri L via Histonet]

Got it...

I need to test it. 

But, I'm going to take some parafilm, pull it over the bolt, then replace the 
screw. 

Increase that friction. 😊

Geri 

-Original Message-
From: Keyser, Geri L via Histonet  
Sent: Wednesday, May 25, 2022 9:59 AM
To: Anne Murvosh ; Ken Marzinsky 
; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat anti-roll plate

Maybe there's a product that increases friction with screw threads, doesn't 
have toxic outgassing, and works at low temperatures. 

I'm going to look for something like this. 

Geri

-Original Message-
From: Anne Murvosh via Histonet  
Sent: Wednesday, May 25, 2022 8:32 AM
To: Ken Marzinsky ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat anti-roll plate

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.

We have 3 new leicas and we constantly have to adjust them. They creep forward 
so much that I just automatically know to roll it slightly back after every 4 
specimens or so. The new leica designs are just bad and customer service is 
even worse. We have to use the plate as we do Mohs specimens and the sections 
are just better with the glass plate. I want to look for a different machine 
next time. We did test the Avantik and it cut nice but the glass plate was on 
the front and got in the way. I wish they would talk to Mohs techs before they 
design these. Oh well. No tricks just constant adjusting. Thanks Anne

-Original Message-
From: Ken Marzinsky via Histonet 
Sent: Tuesday, May 24, 2022 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? 
Does it work well on the Leicas and Microms, or do you have to figit with it a 
lot? Does anyone have any tricks they can share to get it to work all the time? 
 I found that a little spritz of freeze spray on the plate and the bottom of 
the roll glass helps, but it is still seems a lot easier for us to use a brush 
or a teasing needle.

Ken
Durham, NC
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Re: [Histonet] Cryostat anti-roll plate

[Keyser, Geri L via Histonet]

Maybe there's a product that increases friction with screw threads, doesn't 
have toxic outgassing, and works at low temperatures. 

I'm going to look for something like this. 

Geri

-Original Message-
From: Anne Murvosh via Histonet  
Sent: Wednesday, May 25, 2022 8:32 AM
To: Ken Marzinsky ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat anti-roll plate

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.

We have 3 new leicas and we constantly have to adjust them. They creep forward 
so much that I just automatically know to roll it slightly back after every 4 
specimens or so. The new leica designs are just bad and customer service is 
even worse. We have to use the plate as we do Mohs specimens and the sections 
are just better with the glass plate. I want to look for a different machine 
next time. We did test the Avantik and it cut nice but the glass plate was on 
the front and got in the way. I wish they would talk to Mohs techs before they 
design these. Oh well. No tricks just constant adjusting. Thanks Anne

-Original Message-
From: Ken Marzinsky via Histonet 
Sent: Tuesday, May 24, 2022 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? 
Does it work well on the Leicas and Microms, or do you have to figit with it a 
lot? Does anyone have any tricks they can share to get it to work all the time? 
 I found that a little spritz of freeze spray on the plate and the bottom of 
the roll glass helps, but it is still seems a lot easier for us to use a brush 
or a teasing needle.

Ken
Durham, NC
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Re: [Histonet] Cryostat anti-roll plate

[Anne Murvosh via Histonet]

We have 3 new leicas and we constantly have to adjust them. They creep forward 
so much that I just automatically know to roll it slightly back after every 4 
specimens or so. The new leica designs are just bad and customer service is 
even worse. We have to use the plate as we do Mohs specimens and the sections 
are just better with the glass plate. I want to look for a different machine 
next time. We did test the Avantik and it cut nice but the glass plate was on 
the front and got in the way. I wish they would talk to Mohs techs before they 
design these. Oh well. No tricks just constant adjusting. Thanks Anne

-Original Message-
From: Ken Marzinsky via Histonet 
Sent: Tuesday, May 24, 2022 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? 
Does it work well on the Leicas and Microms, or do you have to figit with it a 
lot? Does anyone have any tricks they can share to get it to work all the time? 
 I found that a little spritz of freeze spray on the plate and the bottom of 
the roll glass helps, but it is still seems a lot easier for us to use a brush 
or a teasing needle.

Ken
Durham, NC
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Re: [Histonet] Cryostat anti-roll plate

[Keyser, Geri L via Histonet]

I use roll plate. 

I don't like the roll plate on the Leica. The Leica design creeps... a lot. 

Also, with the leica, the contact point with the blade is a soft aluminum arm. 
If you make adjustments with the roll plate holder contacting the blade, it 
will damage the soft aluminum holder. 

I like the thermo design much better. It creeps a lot less. 

Here's a procedure for roll plate adjustment. 

1.  Mount block on specimen disc. 
2.  Place Specimen Disc in chuck, position Blade carrier, and face off.
3.  Turn knurling screw clockwise until sections do not pass under the 
Anti-roll plate.
4.  Turn knurling screw counter-clockwise by 20 degree increments until 
sections effortlessly pass under the Anti-Roll Plate WITHOUT hitting the block. 

*   If the block is hitting the anti-roll plate, it may cause damage to 
glass. 
*   Hard contact is visible and can be heard. 
*   Soft contact, which can also damage the Anti-roll plate, cannot be seen 
or heard. But, can be felt by gently placing finger on the anti-roll plate 
turning lever while taking a section.


Geri 


-Original Message-
From: Ken Marzinsky via Histonet  
Sent: Tuesday, May 24, 2022 3:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? 
Does it work well on the Leicas and Microms, or do you have to figit with it a 
lot? Does anyone have any tricks they can share to get it to work all the time? 
 I found that a little spritz of freeze spray on the plate and the bottom of 
the roll glass helps, but it is still seems a lot easier for us to use a brush 
or a teasing needle.

Ken
Durham, NC
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Re: [Histonet] Cryostat validation

[Terri Braud via Histonet]

Tony - We were on the same page.  We purchased fresh beef liver, sectioned and 
froze it, cut and stained duplicate blocks and had the pathologist sign off on 
the process.  We were also able to get some tonsils delivered fresh and did 
them, too.  Terri

Terri L. Braud, HT(ASCP)
HNL Laboratories for 
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874
Today's Topics:

   
   2. Re: New cryostat validation procedure (Tony Henwood (SCHN))
Message: 2
Date: Mon, 23 Aug 2021 20:31:19 +
From: "Tony Henwood (SCHN)" 
To: Greg Dobbin 
Subject: Re: [Histonet] New cryostat validation procedure

Hi Greg,

I would just grab some kidneys from the butcher, freeze and section as usual. 
They should give equivalent results to the older unit.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal 
Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of 
Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead 
NSW 2145, AUSTRALIA



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Re: [Histonet] Cryostat

[Colleen Forster via Histonet]

I agree with Terri. I also have an 1850 and will use it until parts are no
longer available which could be,. like she said, years. We also have a
local company do the PM and repairs and will use it until
it absolutely cannot be repaired.

Colleen Forster HT(ASCP)QIHC
University of Minnesota
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 555455
612-626-1930

On Tue, Aug 10, 2021 at 10:47 AM Terri Braud via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> I'm not sure I understand why you would want to replace your 1850s just
> because Leica is closing the support.  I have 2 and they are real
> workhorses.  There are 2 independent companies in the area that can repair
> and perform PMs.  One is Bel-Air and the other is Advantik.  I'm sure there
> are others, but I've used both companies and can highly recommend them.  If
> you like your Leica, keep it until parts are no longer available, which
> could be years.
> I have two 1850s and I'm sure they will be here for a long time.  Terri
>
> Terri L. Braud, HT(ASCP)
> HNL Laboratories for
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> Ph: 215-938-3689
> Fax: 215-938-3874
> Today's Topics:
>
>1. cryostat (Histology)
>
> Date: Mon, 9 Aug 2021 13:00:30 +
> Subject: [Histonet] cryostat
> Hello,
> Trying to get everyone's opinion on cryostats.  We have had the Leica
> CM1850 forever and it is not being supported by Leica anymore.  Thinking of
> trying a different company maybe Tanner or Rankin.  What does everyone use
> and like.  We do not cut Mohs, only DIFs.  Maybe 1 a day.
> Thanks to all,
> Mehndi Helgren
> Dominion Pathology Laboratories
> 733 Boush Street
> Suite 200
> Norfolk, VA 23510
> 757-664-7901
>
>
>
> ----------
>
> Message: 2
> Date: Mon, 9 Aug 2021 13:56:42 +
> From: "Morken, Timothy" 
> To: Histology 
> Cc: Histonet 
> Subject: Re: [Histonet] cryostat
> Message-ID:
> <
> co1pr05mb8053d300dfe09b8f30e66acbe7...@co1pr05mb8053.namprd05.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We
> have two Epredia NX70 cryostats. We've used them for 8 years now and really
> like them.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies Department
> of Pathology UC San Francisco Medical Center
>
>
> -Original Message-
> From: Histology via Histonet 
> Sent: Monday, August 09, 2021 6:01 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] cryostat
>
> Hello,
>
> Trying to get everyone's opinion on cryostats.  We have had the Leica
> CM1850 forever and it is not being supported by Leica anymore.  Thinking of
> trying a different company maybe Tanner or Rankin.  What does everyone use
> and like.  We do not cut Mohs, only DIFs.  Maybe 1 a day.
>
> Thanks to all,
>
> Mehndi Helgren
>
> Dominion Pathology Laboratories
> 733 Boush Street
> Suite 200
> Norfolk, VA 23510
> 757-664-7901
>
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>
>
>
> --
>
> Subject: Digest Footer
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>
> End of Histonet Digest, Vol 213, Issue 6
> 
>
>
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-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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Re: [Histonet] Cryostat

[Terri Braud via Histonet]

I'm not sure I understand why you would want to replace your 1850s just because 
Leica is closing the support.  I have 2 and they are real workhorses.  There 
are 2 independent companies in the area that can repair and perform PMs.  One 
is Bel-Air and the other is Advantik.  I'm sure there are others, but I've used 
both companies and can highly recommend them.  If you like your Leica, keep it 
until parts are no longer available, which could be years.
I have two 1850s and I'm sure they will be here for a long time.  Terri

Terri L. Braud, HT(ASCP)
HNL Laboratories for 
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874
Today's Topics:

   1. cryostat (Histology)

Date: Mon, 9 Aug 2021 13:00:30 +
Subject: [Histonet] cryostat
Hello,
Trying to get everyone's opinion on cryostats.  We have had the Leica CM1850 
forever and it is not being supported by Leica anymore.  Thinking of trying a 
different company maybe Tanner or Rankin.  What does everyone use and like.  We 
do not cut Mohs, only DIFs.  Maybe 1 a day.
Thanks to all,
Mehndi Helgren
Dominion Pathology Laboratories
733 Boush Street
Suite 200
Norfolk, VA 23510
757-664-7901



--

Message: 2
Date: Mon, 9 Aug 2021 13:56:42 +
From: "Morken, Timothy" 
To: Histology 
Cc: Histonet 
Subject: Re: [Histonet] cryostat
Message-ID:



Content-Type: text/plain; charset="us-ascii"

Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We have two 
Epredia NX70 cryostats. We've used them for 8 years now and really like them. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center


-Original Message-
From: Histology via Histonet 
Sent: Monday, August 09, 2021 6:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat

Hello,

Trying to get everyone's opinion on cryostats.  We have had the Leica CM1850 
forever and it is not being supported by Leica anymore.  Thinking of trying a 
different company maybe Tanner or Rankin.  What does everyone use and like.  We 
do not cut Mohs, only DIFs.  Maybe 1 a day.

Thanks to all,

Mehndi Helgren

Dominion Pathology Laboratories
733 Boush Street
Suite 200
Norfolk, VA 23510
757-664-7901

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Subject: Digest Footer

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End of Histonet Digest, Vol 213, Issue 6



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Re: [Histonet] cryostat

[Haiyan via Histonet]

Hi Mehndi,

Same here, I had been using Epredia cryostat for the last several years. I
like it very much.  A video on youtube shows its beautiful features.
https://youtu.be/oMNt6RT5cWI

Best,
Haiyan

On Mon, Aug 9, 2021 at 7:04 AM Morken, Timothy via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We
> have two Epredia NX70 cryostats. We've used them for 8 years now and really
> like them.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
> -Original Message-
> From: Histology via Histonet 
> Sent: Monday, August 09, 2021 6:01 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] cryostat
>
> Hello,
>
> Trying to get everyone's opinion on cryostats.  We have had the Leica
> CM1850 forever and it is not being supported by Leica anymore.  Thinking of
> trying a different company maybe Tanner or Rankin.  What does everyone use
> and like.  We do not cut Mohs, only DIFs.  Maybe 1 a day.
>
> Thanks to all,
>
> Mehndi Helgren
>
> Dominion Pathology Laboratories
> 733 Boush Street
> Suite 200
> Norfolk, VA 23510
> 757-664-7901
>
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>
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Re: [Histonet] cryostat

[Morken, Timothy via Histonet]

Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We have two 
Epredia NX70 cryostats. We've used them for 8 years now and really like them. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Histology via Histonet  
Sent: Monday, August 09, 2021 6:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat

Hello,

Trying to get everyone's opinion on cryostats.  We have had the Leica CM1850 
forever and it is not being supported by Leica anymore.  Thinking of trying a 
different company maybe Tanner or Rankin.  What does everyone use and like.  We 
do not cut Mohs, only DIFs.  Maybe 1 a day.

Thanks to all,

Mehndi Helgren

Dominion Pathology Laboratories
733 Boush Street
Suite 200
Norfolk, VA 23510
757-664-7901

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Re: [Histonet] Cryostat

[MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST) via Histonet]

Hi Brittany,
Personally I do not like the HM525NX.  It is awkward and difficult to control 
with no manual advance except the control panel.  When advancing /trimming you 
are never certain how far forward the block will travel.  The UV light fitting 
in the lid always seems to get in the way of my field of view.  Ours doesn't 
have temperature control of the block holder which can make sectioning more 
difficult.

Looking at the specs of the cryostar I would actually opt for the NX70.

Kind regards,
Penny :)

Penny Marr
Senior BMS

C/- Histology
Conquest Hospital
St Leonards-on-Sea TN37 7RD

penny.m...@nhs.net

0300 131 4500 ext 734914
0300 131 4914

-Original Message-
From: Brittany Hethcox [mailto:bheth...@hcmhcares.org]
Sent: 19 May 2021 15:21
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat

Hello everyone!

I am new to the Histology world. You all may know my previous coworker,
Rhonda Ford. She officially retired and I am so happy for her! With that
being said, I am in a pickle because the cryostat we had completely broke
down. We were having issues with it during my training period, so I never
officially got to use it. We are now looking for a new cryostat for our
department. My supervisor is asking me about different models and features,
but I don't know much about any of this. So I was hoping you all could
help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically
the "Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones
will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our
purchase (e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

--
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

--
*"We Make Lasting Connections"*Henry Community Health





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Re: [Histonet] Cryostat

[Morken, Timothy via Histonet]

Guess I missed your other questions. 

I think the fog mist (Cold D) may be better - it reaches all area of the 
chamber. UV light is shaded from some areas, though probably hits most areas 
you might be touching. 

The misting requires a specific solution, not just anything. Hydrogen peroxide 
is the disinfecting agent. 

Other features:
We have the vacutome on the original units but we never use it. We do all 
kidney and muscle sectioning and these small samples do not produce much 
debris. On our new replacement unit (to replace a 7-year old NX70) we opted out 
of the vacuum.  If using in a gross room/Or setting the vacuum may be very 
useful. It only adds a hose and suction manifold in the unit and does not get 
in the way of anything. 




Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Morken, Timothy via Histonet  
Sent: Wednesday, May 19, 2021 8:26 AM
To: Brittany Hethcox 
Cc: Histonet 
Subject: Re: [Histonet] Cryostat

Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're 
reliable and easy to use. We have the elevator on ours and it makes a 
difference for different size people in the lab. It also can have a vapor 
disinfectant system which we use and it works great. 

We use ours for kidney and muscle cryosectioning, not gross room/OR sections 
but these would work fine for that. They have a lot of room inside for 
specimens. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center


-Original Message-
From: Brittany Hethcox via Histonet 
Sent: Wednesday, May 19, 2021 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat

Hello everyone!

I am new to the Histology world. You all may know my previous coworker, Rhonda 
Ford. She officially retired and I am so happy for her! With that being said, I 
am in a pickle because the cryostat we had completely broke down. We were 
having issues with it during my training period, so I never officially got to 
use it. We are now looking for a new cryostat for our department. My supervisor 
is asking me about different models and features, but I don't know much about 
any of this. So I was hoping you all could help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically the 
"Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones 
will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our purchase 
(e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

--
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

--
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Re: [Histonet] Cryostat

[Morken, Timothy via Histonet]

Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're 
reliable and easy to use. We have the elevator on ours and it makes a 
difference for different size people in the lab. It also can have a vapor 
disinfectant system which we use and it works great. 

We use ours for kidney and muscle cryosectioning, not gross room/OR sections 
but these would work fine for that. They have a lot of room inside for 
specimens. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Brittany Hethcox via Histonet  
Sent: Wednesday, May 19, 2021 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat

Hello everyone!

I am new to the Histology world. You all may know my previous coworker, Rhonda 
Ford. She officially retired and I am so happy for her! With that being said, I 
am in a pickle because the cryostat we had completely broke down. We were 
having issues with it during my training period, so I never officially got to 
use it. We are now looking for a new cryostat for our department. My supervisor 
is asking me about different models and features, but I don't know much about 
any of this. So I was hoping you all could help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically the 
"Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones 
will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our purchase 
(e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

--
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

--
*"We Make Lasting Connections"*Henry Community Health 
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Re: [Histonet] Cryostat cooling time

[Lynn Whitmore via Histonet]

The amount of time a cryostat takes to get to cutting temperature depends on 
the age of the machine, manufacturer, and air flow around the cryostat. We have 
4 Avantiks the newer ones cool faster than the older ones and the one that is 
under a wall cabinet with about 2 feet from the top of the cryostat to the 
bottom of the cabinet takes about an hour longer to cool.  The cryostats are 
kept at -27. The cooling time for the quicker one is 2 hours from room 
temperature (70 degrees Fahrenheit) and the slower one is 3 hours.

I would suggest you plan for 4 hours and monitor the cooling time so you know 
for the next time you need to shutdown the cryostat. I have had Leicas and 
Thermo Fisher cryostats and they were different. Hope this helps.



-Original Message-
>From: histonet-requ...@lists.utsouthwestern.edu
>Sent: Sep 5, 2019 1:00 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: Histonet Digest, Vol 190, Issue 3
>
>Send Histonet mailing list submissions to
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>Today's Topics:
>
>   1. Re: CPT Code 88172 (Webster, Thomas S.)
>   2. Re: CPT Code 88172 (Joe W. Walker, Jr.)
>   3. Re: CPT Code 88172 (Victoria Baker)
>   4. PPE for frozen sections (Moe, Barbi A)
>   5. DAKO Artisan special stain kit validations (Moe, Barbi A)
>   6. Cryostat to room temperature (Moe, Barbi A)
>
>
>--
>
>Message: 1
>Date: Wed, 4 Sep 2019 17:31:03 +
>From: "Webster, Thomas S." 
>To: "'histonet@lists.utsouthwestern.edu'"
>   
>Subject: Re: [Histonet] CPT Code 88172
>Message-ID: 
>Content-Type: text/plain; charset="us-ascii"
>
>You can only bill for 88172 if the pathologist looks at the FNA slide(s) 
>during the procedure.  Otherwise do not attempt to bill for it.  It is a 
>physician code.
>
>
>CONFIDENTIALITY NOTICE:
>This e-mail message, including all attachments, is for the sole use of the
>intended recipient(s) and may contain confidential and privileged
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>information.  If you are not the intended recipient, please contact the
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>
>--
>
>Message: 2
>Date: Wed, 4 Sep 2019 18:07:26 +
>From: "Joe W. Walker, Jr." 
>To: "Webster, Thomas S." 
>Cc: "histonet@lists.utsouthwestern.edu"
>   
>Subject: Re: [Histonet] CPT Code 88172
>Message-ID:
>   
> 
>   
>Content-Type: text/plain; charset="utf-8"
>
>Hi Thomas,
>
>What is your reference source for stating this is "a physician code"?  In 
>reviewing our coding data, this code carries a facilities component in 
>addition to a professional component.  I agree that a pathologist must perform 
>the review in order to bill for both, but I am aware of people in New England 
>who bill just the TC when a technologist provides the adequacy interpretation 
>and some payers cover this charge.
>
>Joe W. Walker, Jr. MS, SCT(ASCP)
>Anatomical Pathology Manager
>joewal...@rrmc.org, www.rrmc.org
>
>-Original Message-
>From: Webster, Thomas S. via Histonet 
>Sent: Wednesday, September 4, 2019 1:31 PM
>To: 'histonet@lists.utsouthwestern.edu' 
>Subject: Re: [Histonet] CPT Code 88172
>
>[External Email] This email originated from outside of the organization. Think 
>before you click: Don?t click on links, open attachments or respond to 
>requests for sensitive information if the email looks suspicious or you don?t 
>recognize the sender.
>
>
>You can only bill for 88172 if the pathologist looks at the FNA slide(s) 
>during the procedure.  Otherwise do not attempt to bill for it.  It is a 
>physician code.
>
>
>CONFIDENTIALITY NOTICE:
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Re: [Histonet] cryostat decontamination

[Duddey, Aimee via Histonet]

Our last discussion with TJC to clarify their definition of "regular interval" 
was to follow the manufacturers guidelines.  I recommend determining what 
manufacturer suggests with the use of the UV decon and write that in your 
policy that addresses this standard.  If you are following their guidelines, 
you should be in compliance.

Aimee

-Original Message-
From: Nancy Schmitt via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, August 15, 2016 9:58 AM
To: '
Subject: [Histonet] cryostat decontamination

ANP.23410 states that cryostat is defrosted and wiped down at regular 
intervals.  What if you have UV decontam?  Is there a ruling on that?

Thanks

Nancy Schmitt MLT, HT(ASCP)
Dubuque, IA

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Re: [Histonet] Cryostat Chirp

[Elizabeth Chlipala via Histonet]

Gary

I think that might have to do with the fan motor inside the unit, it might need 
to be replaced.   We had ours replaced a few years ago.  Do you service/PM the 
cryostat yearly?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Martin, Gary via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, May 03, 2016 2:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Chirp

My Leica CM 1850 cryostat is making a chirping sound and I can't figure out 
what is doing this. Has anyone experienced this sound, if what was the resolve. 

Thanks 

Gary

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Re: [Histonet] Cryostat with UV disinfection

[Pfaff, Karen via Histonet]

Our Mohs Lab is CAP accredited. Every seven  days of use, cryostats are shut 
down. Once machine has been cleaned and dried we use Cavi-wipes(super 
Sani-Cloth from PDI Inc.)  We then document disinfection date on QC Log.   We 
have three cryostats, we rotate a cryostat a week for disinfecting purposes. We 
added this to our Equipment: Cryostats/Microtomes policy.
Hope this helps.
Karen Pfaff, HT (ASCP), BA
Lead Histotechnician
Skin Cancer Center
Froedtert Health
Phone: 414-805-6010    Fax: 414-805-5325


-Original Message-
From: Terri Braud [mailto:tbr...@holyredeemer.com] 
Sent: Friday, March 11, 2016 12:52 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Cryostat with UV disinfection

For those of you that are CAP accredited, and use a cryostat with UV 
disinfection, how do you get around the CAP requirement listed below? We were 
told the UV disinfection did not replace wiping down with a tuberculocidal 
disinfectant at room temp. Do you have an approved tuberculocidal disinfectant 
that will work at cryostat temps?  Curious minds would like to know!  
ANP.23410   Cryostat DecontaminationPhase II
There is a written procedure for the decontamination of the cryostat at defined 
intervals, and under defined circumstances, and decontamination records are 
evident.
NOTE:  The cryostat must be defrosted and decontaminated by wiping all exposed 
surfaces with tuberculocidal disinfectant. The cryostat should be at room 
temperature during decontamination unless otherwise specified by the 
manufacturer. This should be done at an interval appropriate for the 
institution; this must be weekly for instruments used daily. Trimmings and 
sections of tissue that accumulate inside the cryostat must be removed during 
decontamination. Although not a requirement, steel mesh gloves should be worn 
when changing knife blades. 
Terri L. Braud, HT(ASCP)





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Re: [Histonet] Cryostat with UV disinfection

[Terri Braud via Histonet]

For those of you that are CAP accredited, and use a cryostat with UV 
disinfection, how do you get around the CAP requirement listed below? We were 
told the UV disinfection did not replace wiping down with a tuberculocidal 
disinfectant at room temp. Do you have an approved tuberculocidal disinfectant 
that will work at cryostat temps?  Curious minds would like to know!  
ANP.23410   Cryostat DecontaminationPhase II
There is a written procedure for the decontamination of the cryostat at defined 
intervals, and under defined circumstances, and decontamination records are 
evident.
NOTE:  The cryostat must be defrosted and decontaminated by wiping all exposed 
surfaces with tuberculocidal disinfectant. The cryostat should be at room 
temperature during decontamination unless otherwise specified by the 
manufacturer. This should be done at an interval appropriate for the 
institution; this must be weekly for instruments used daily. Trimmings and 
sections of tissue that accumulate inside the cryostat must be removed during 
decontamination. Although not a requirement, steel mesh gloves should be worn 
when changing knife blades. 
Terri L. Braud, HT(ASCP)



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Re: [Histonet] Cryostat troublshooting. CM3050

[Hans B Snyder]

Hello Patrick,

We have this same cryostat except it's the CM 3050S.  Ours also has
this issue usually after it's been running for many months with a
defrost.  The typical cause of your problem is the cylinder on which
the block holder is attached has ice crystals or is "frozen".  I feel
the cause of this is because the glass was left open too long while
cutting.  This allowed moister from the room to condense on the
cylinder and subsequently causes a stop go motion.

 Since I am in charge of all histology equipment maintenance, my
solution is to turn off the cryostat, take the microtome out of the
cavity and let it dry overnight.  Then in the morning, I physically
dry the microtome.  Using cryostat oil, oil all the moving parts
including the cylinder, then wipe off excess.  Before putting the
microtome back into the cavity, the cavity itself must also be
thoroughly dried.  The final cool down process usually takes about 1-2
hours.

Feel free to email any specific questions.

Hope this helps.
Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com


On Wed, Jan 7, 2015 at 6:42 PM, Lewis, Patrick
 wrote:
> H everyone,
>
> We have been using this old CM3050 cryostat for our Frozen OCT blocks.
>
> I am cutting at 5 uM.
>
> My issue is that when I use the hand rotator to cut my sections it doesn't 
> seem to advance with each rotation.  It can take as many as 4 rotations to go 
> from one section to another.
>
> What would cause this, and is there an easy solution to this problem?
>
> I can use the button to automatically move the chick holder all the forward 
> and back from blinking to blinking, so I am wondering if ice crystals or 
> debris can be ruled out as  the issue.
>
> Any advice welcome.
>
> Thanks
>
> Patrick.
>
> ps: when I do get the blade to cut into the block, I have to wonder if the 
> section I am getting is really 5 uM, or is it thicker.  Sometimes you can 
> tell if the section looks particularly thick, but other times it's hard to 
> tell.
>
>
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> disclosure or distribution is prohibited. If you are not the intended 
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Re: [Histonet] cryostat design

[Alan Bright]

Dear Kim,

Yes the Starlet cryostat made by Bright Instrument Company, it is in a small 
bench level cabinet as there is no other way of achieving good results on the 
refrigeration. Apart from freezing microtones which were highly inefficient on 
retrieving good quality thin sections.
Hope this assists you.

Alan Bright
BIC..UK

Sent from my iPhone

> On 5 Mar 2014, at 16:55, "Kolman, Kimberly D."  wrote:
> 
> Hello all;
> 
> Is anyone aware of a cryostat design in which the microtome cabinet
> would sit at bench level?  In other words, it would function like any
> regular bench microtome but be housed in a cold cabinet like a cryostat?
> This would enable the operator to work in a desk position instead of
> uncomfortably being hunched over a cryostat cabinet.  
> 
> 
> 
> Thanks in advance,
> 
> Kim
> 
> 
> 
> 
> 
> Kimberly D. Kolman, HT  (ASCP)
> 
> VA Eastern Kansas Health Care System
> 
> 4101 S 4th St Trfwy
> 
> Leavenworth KS 66048
> 
> 913-682-2000x 52537
> 
> Fax: 913-758-4193
> 
> 
> 
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> 
> -- 
> 

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Re: [Histonet] Cryostat

[Hugh Luk]

Tanya and Madeline,

Jan Minshew of Leica sent a nicely detailed response to "Decontaminating" a 
cryostat in 
January of 2010. She apparently wrote it to put in a Leica "How To" manual, 
although I did
not notice it in this form.  Everything she states is well documented, although 
I also 
incorporate a known virucide and tuberculocidal product (following it's 
directions, naturally).

http://lists.utsouthwestern.edu/pipermail/histonet/2010-January/048750.html

If you have a UV cryostat, use Leica's Cryofect product or follow Tim Morken's 
previous
(2000 histosearch)formaldehyde method, they should work for killing most bugs 
too(with the 
exception of eradicating some prions like CJD/BSE).


If I am misunderstanding what you mean by "Decon" I apologize, as there is no 
reason why
you cannot clean up tissue debris in a cold cryostat with 100% alcohol.

Hugh
Hawaii

--
 
Date: Thu, 16 Jan 2014 10:43:09 -0800 (PST)
From: Madeline Gi 
Subject: Re: [Histonet] Cryostat
To: "Abbott, Tanya" ,
"Histonet@lists.utsouthwestern.edu"

Message-ID:
<1389897789.16602.yahoomail...@web121504.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
 
I use to decon my cryostat with 100% Alco this keeps it from freezing up and it 
clean it. 
best of luck. 
 
Madeline Rotger Milanese H.T. BSHCS
500 New Hempstead Rd.
New City N.Y. 10965
845-362-3200 Ext 129
madelin...@yahoo.com
 
 
 
On Wednesday, January 15, 2014 2:52 PM, "Abbott, Tanya" 
 wrote:
 
Need to decon my cryostat
 
Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net
 
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Re: [Histonet] Cryostat

[Madeline Gi]

I use to decon my cryostat with 100% Alco this keeps it from freezing up and it 
clean it. 
best of luck. 
 
Madeline Rotger Milanese H.T. BSHCS
500 New Hempstead Rd.
New City N.Y. 10965
845-362-3200 Ext 129
madelin...@yahoo.com



On Wednesday, January 15, 2014 2:52 PM, "Abbott, Tanya" 
 wrote:
 
Need to decon my cryostat

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

This electronic mail and any attached documents are intended solely for the 
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received this email in error and are notified that reading, copying, or 
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RE: [Histonet] cryostat service

[Walter Benton]

Bel Air Instruments and Dolbey Jamison

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni 
[trathbo...@somerset-healthcare.com]
Sent: Monday, October 28, 2013 2:29 PM
To: 'Jean Ross'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat service

Avantik is located in NJ and services cryostats in addition to microtomes, 
processors and stainers.
www.avantik-us.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Ross
Sent: Monday, October 28, 2013 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat service

Hi everyone,

I am interested in finding out if there is anyone in or close to Delaware that 
services and repairs cryostats.  Any recommendations would be much appreciated.

Thanks,
Jean
--
Jean Ross
Delaware Biotechnology Institute, BioImaging Center University of Delaware
15 Innovation Way
Suite 117
Newark, DE  19711
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RE: [Histonet] cryostat service

[Rathborne, Toni]

Avantik is located in NJ and services cryostats in addition to microtomes, 
processors and stainers.
www.avantik-us.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Ross
Sent: Monday, October 28, 2013 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat service

Hi everyone,

I am interested in finding out if there is anyone in or close to Delaware that 
services and repairs cryostats.  Any recommendations would be much appreciated.

Thanks,
Jean
--
Jean Ross
Delaware Biotechnology Institute, BioImaging Center University of Delaware
15 Innovation Way
Suite 117
Newark, DE  19711
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RE: [Histonet] cryostat service

[Barbara Tibbs]

Hello,  

BelAir is an excellent repair service company located in NJ.  They will come to 
Delaware for repairs.  Look them up online for the contact information.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of Jean Ross 

Sent: Monday, October 28, 2013 4:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat service

Hi everyone,

I am interested in finding out if there is anyone in or close to Delaware
that services and repairs cryostats.  Any recommendations would be much
appreciated.

Thanks,
Jean
--
Jean Ross
Delaware Biotechnology Institute, BioImaging Center
University of Delaware
15 Innovation Way
Suite 117
Newark, DE  19711
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RE: [Histonet] Cryostat cleaning

[Hannen, Valerie]

We wipe out the excess tisue( if there is any),  wipe down the chamber with 
abs. Alcohol then spray the chamber with "CaviCide", let it sit for @ 3-5 
minutes and then wipe the chamber out again.


Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Friday, August 16, 2013 11:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat cleaning


ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a 
tuberculocidal.

Can someone tell me what they use that meets the CAP criteria listed above.

thankis,
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RE: [Histonet] Cryostat cleaning

[Morken, Timothy]

Ann, 

There are many tuberculocidal cleaners. Just search for it. If you are in a 
hospital the central supply will most likely have some in stock.  
We use PD-128 by Spartan Chemical  

http://www.spartanchemical.com/products/product/101604


Here are some references for cryostat cleaning:

Cryostat decontamination references:

College of American Pathologists, Commentary on certification questionnaire:
1)  Decontaminate regularly with 70% alcohol.
2)  Defrost, remove trimmings and decontaminate with a tuberculocidal 
disinfectant, preferably weekly for instruments used daily
3)  Reference: National Committee for Clinical Laboratory Standards 
(NCCLS), Protection of Laboratory Workers from Instrument Biohazards and 
Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved 
Guideline M29-A. Wayne, PA, NCCLS, 1997

NCCLS site: http://www.nccls.org/


Decontamination for HIV
Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., 
Ewing, E.P., J histotechnol, V9, No.1, p.29 (March 1986)
Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is 
effective against HIV).

Department of Agriculture for Northern Ireland, Safe Working and the Prevention 
of Infection in Clinical Laboratories, Health Services Advisory Committee. 
Recommends formalin fuming for 24-48 hours at room temperature. Followed by 
ammonia for one hour (presumably as a neutralizer).

Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill 
Livingstone, 1994.
Recommends defrosting , washing with 70% alcohol, complete cleaning. (no 
references given).

For those who like to read a lot:
APIC Guideline for Selection and Use of Disinfectants, Association for 
Professionals in Infection Control (APIC), American Journal of Infection 
Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in 
use and the pros and cons of each.


Tim Morken
UCSF Medical Center

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Friday, August 16, 2013 8:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat cleaning


ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a 
tuberculocidal.

Can someone tell me what they use that meets the CAP criteria listed above.

thankis, 
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Re: [Histonet] Cryostat recommendations

[Dmitry Kislov]

Hi,

Leica 1850s are very reliable, 1860 is basically the same unit with very
precise electronic specimen feed.
UV lamp's lifetime is about 8k hours, and every switch on cycle adds
another hour to the counter. New lamp will cost you a few hundred euro,
though. Any operator can replace the lamp, no need for service call.

Dmitry,
Sr. engineer
Moscow region pathology bureau

On Fri, Aug 9, 2013 at 3:44 PM, Rathborne, Toni <
trathbo...@somerset-healthcare.com> wrote:

> Happy Friday everyone!
> I'm looking for some feedback on cryostat preferences. We are replacing
> one of ours now and will be evaluating a few before making a final
> decision. The 2 I've looked into so far are the Leica 1860 and the HM525 by
> Thermo Scientific. I'm interested in the UV feature that many now offer too
> and want to know what your experiences are. Does it require extra
> maintenance? Is it costly to repair/replace?  Do either of these models (or
> previous versions) have any known problems? They both look very nice on the
> outside, but are they made of durable materials internally? Thanks in
> advance for your responses, and have a great weekend!
>
> Toni
>
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RE: [Histonet] Cryostat Cleaning

[Morken, Timothy]

Ann,

Cryostat decontamination references:

 

College of American Pathologists, Commentary on certification questionaire:

1) Decontaminate regularly with 70% alcohol.

2) Defrost, remove trimmings and decontaminate with a tuberculocidal 
disinfectant, preferably weekly for instruments used daily

3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), 
Protection of Laboratory Workers from Instrument Biohazards and Infectious 
Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline 
M29-A. Wayne, PA, NCCLS, 1997

http://www.cap.org/HTML/checklist_html/cklst08p.html

 

NCCLS site: http://www.nccls.org/

 

 

Decontamination for HIV

Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., 
Ewing, E.P., J histotechnol, V9, No.1, p.29 (March 1986)

Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is 
effective against HIV).

 

Department of Agriculture for Northern Ireland, Safe Working and the Prevention 
of Infection in Clinical Laboratories, Health Services Advisory Committee. 

Recommends formalin fuming for 24-48 hours at room temperature. Followed by 
ammonia for one hour (presumably as a neutralizer).

 

Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill 
Livingstone, 1994.

Recommends defrosting , washing with 70% alcohol, complete cleaning. (no 
references given).

 

For those who like to read a lot:

APIC Guideline for Selection and Use of Disinfectants, Association for 
Professionals in Infection Control (APIC), American Journal of Infection 
Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in 
use and the pros and cons of each.


Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Tuesday, August 06, 2013 12:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Cleaning


Can someone tell me the CAP approved chemical that can be used to clean the 
cryostat?
thanks, Ann
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RE: [Histonet] cryostat

[Jones, Laura]

Hi Irena!

If you go to IMEB Inc.'s website, and type "vacuum" into the search, it will 
take you to what we use.  They sell a vacuum cleaner and accessories.  We 
currently use the filtered hoses with an old canister vacuum that's been around 
longer than I have.  When the filter gets full, we discard it in the biohazard 
trash and replace it with a new one.

Hope this helps!

Laura


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS 
[irena.kir...@hotmail.com]
Sent: Wednesday, February 06, 2013 8:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat

Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in 
other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw 
this in one lab!?thanksIrena 
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RE: [Histonet] cryostat

[Elizabeth Chlipala]

Happy Wednesday Everyone

There are vacumes specifically made for cryostats that are hepa filtered, etc.  
We have one of those.  The company is called MARMED.  We use a shop vac for the 
paraffin microtomes, that works nicely.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alan Bright 
[abri...@brightinstruments.com]
Sent: Wednesday, February 06, 2013 9:31 AM
To: IRENA SREBOTNIK KIRBIS
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] cryostat

I would not vacuum the trimmings out of cryostat, if it were infected It would 
make a good job of contaminating a laboratory etc.
Terrified Alan Bright
Sent from my iPhone



>
>
>
>
> Hi,I wonder what is the way of removing shavings/trimmings from the cryostat 
> in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I 
> saw this in one lab!?thanksIrena 
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>
>
> --
>

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Re: [Histonet] cryostat

[Alan Bright]

I would not vacuum the trimmings out of cryostat, if it were infected It would 
make a good job of contaminating a laboratory etc.
Terrified Alan Bright
Sent from my iPhone



> 
> 
> 
> 
> Hi,I wonder what is the way of removing shavings/trimmings from the cryostat 
> in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I 
> saw this in one lab!?thanksIrena 
> ___
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> 
> 
> -- 
> 

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RE: [Histonet] Cryostat Maintenance and Repair - CA

[Laurie Colbert]

Hi Eric,

Mikron Instruments services most of our instruments, including Micron
brand.  I don't have any Leica equipment now, but they have serviced my
Leica microtome in the past.  They are very good!  The service manager's
name is Steve Pike.  Mikron is in Vista, CA and their phone number is
(800) 377-5395.

Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric
Velazquez
Sent: Wednesday, May 04, 2011 2:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Maintenance and Repair - CA

Hi,

Can somebody please recommend to me a company that does cryostat
maintenance
and repair? I am looking for a company that is familiar with both Micron
and
Leica models in the Irvine, CA area.

Thanks,
Eric
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RE: [Histonet] cryostat repair--thanks!

[Weems, Joyce]

The Tech One guy would do the same thing for much cheaper. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Tuesday, April 05, 2011 10:11
To: Victor Tobias; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] cryostat repair--thanks!

The compressor fan was loose, so when the Tech One guy fixed it, instead of 
making a horrible noise when it turns off, it just rattles.  Oh well, time for 
a new motor.
Which leads me to another question, is there any reason I should call Lecia to 
replace this motor? The guy from Tech One Biomedical seemed to know his stuff, 
but my boss seems to think we should call the people who made the cryostat.  
Personally, I think that would be spending a lot more money for the same thing, 
but maybe I'm missing something.  Any suggestions?
By the way, the cryostat is from 2008 and isn't under warranty.

Emily


A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Fri, Apr 1, 2011 at 3:15 PM, Victor Tobias < 
vic...@pathology.washington.edu> wrote:

> So what was the problem?
>
> Victor Tobias HT(ASCP)
> Clinical Applications Analyst
> University of Washington Medical Center Dept of Pathology Room BB220
> 1959 NE Pacific
> Seattle, WA 98195
> vic...@pathology.washington.edu
> 206-744-2735
> 206-744-8240 Fax
> =
> Privileged, confidential or patient identifiable information may be 
> contained in this message. This information is meant only for the use 
> of the intended recipients. If you are not the intended recipient, or 
> if the message has been addressed to you in error, do not read, 
> disclose, reproduce, distribute, disseminate or otherwise use this 
> transmission. Instead, please notify the sender by reply e-mail, and 
> then destroy all copies of the message and any attachments.
>
>
>
> On 4/1/2011 12:09 PM, Emily Sours wrote:
>
>> Thanks to all who answered my email, I ended up calling Tech One 
>> Biomedical and they came right out.  I recommend them to anyone who 
>> needs histology equipment repaired!!
>>
>> Emily
>> ps. this is not an april fool's joke.
>> pps. really!
>>
>> It has become almost a cliche to remark that nobody boasts of 
>> ignorance of literature, but it is socially acceptable to boast 
>> ignorance of science and proudly claim incompetence in mathematics.
>> -Richard Dawkins
>> ___
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>>
>
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It may contain information that is privileged and 
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Re: [Histonet] cryostat repair--thanks!

[Emily Sours]

The compressor fan was loose, so when the Tech One guy fixed it, instead of
making a horrible noise when it turns off, it just rattles.  Oh well, time
for a new motor.
Which leads me to another question, is there any reason I should call Lecia
to replace this motor? The guy from Tech One Biomedical seemed to know his
stuff, but my boss seems to think we should call the people who made the
cryostat.  Personally, I think that would be spending a lot more money for
the same thing, but maybe I'm missing something.  Any suggestions?
By the way, the cryostat is from 2008 and isn't under warranty.

Emily


A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Fri, Apr 1, 2011 at 3:15 PM, Victor Tobias <
vic...@pathology.washington.edu> wrote:

> So what was the problem?
>
> Victor Tobias HT(ASCP)
> Clinical Applications Analyst
> University of Washington Medical Center
> Dept of Pathology Room BB220
> 1959 NE Pacific
> Seattle, WA 98195
> vic...@pathology.washington.edu
> 206-744-2735
> 206-744-8240 Fax
> =
> Privileged, confidential or patient identifiable information may be
> contained in this message. This information is meant only for the use
> of the intended recipients. If you are not the intended recipient, or
> if the message has been addressed to you in error, do not read,
> disclose, reproduce, distribute, disseminate or otherwise use this
> transmission. Instead, please notify the sender by reply e-mail, and
> then destroy all copies of the message and any attachments.
>
>
>
> On 4/1/2011 12:09 PM, Emily Sours wrote:
>
>> Thanks to all who answered my email, I ended up calling Tech One
>> Biomedical
>> and they came right out.  I recommend them to anyone who needs histology
>> equipment repaired!!
>>
>> Emily
>> ps. this is not an april fool's joke.
>> pps. really!
>>
>> It has become almost a cliche to remark that nobody boasts of ignorance of
>> literature, but it is socially acceptable to boast ignorance of science
>> and
>> proudly claim incompetence in mathematics.
>> -Richard Dawkins
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
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RE: [Histonet] cryostat repair

[Bernice Frederick]

We use them too- Illinois office.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bouchal,
Rena L
Sent: Thursday, March 31, 2011 1:38 PM
To: Emily Sours; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat repair

We are using Tech One Biomedical... service reps in Pghfor almost all of
our routine instrument service really good and half the price of the
vendors!  Their number is 866-497-3033 or www.techoneweb.com

Please note that my email address  as of Jan 3, 2011 is
bouch...@wvuhealthcare.com  .   Please make the appropriate changes in
your address book.

Rena Bouchal, M.S.
Anatomic Pathology Manager
West Virginia University Hospitals
304-293-7765

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DANIEL
HEWITT
Sent: Thursday, March 31, 2011 2:15 PM
To: Emily Sours; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat repair

Emily
You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and
do most of our repairs. Ray does an excellent job at a good price.
It sounds like the bearings in your fan or maybe the compressor.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

This email, including any attachments, is for the sole use of the intended
recipient(s) and may contain confidential and privileged information.  Any
unauthorized review, use, disclosure or distribution is prohibited.  If you
are not the intended recipient, or an agent responsible for delivering the
message to the intended recipient, please contact the sender by reply e-mail
and delete and destroy all copies of the original message, including
attachments.

Please note that any views or opinions presented in this e-mail are solely
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Valley Health System.  The integrity and security of this message cannot be
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Thursday, March 31, 2011 1:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat repair

Our cryostat (CM3050S) is making a terrible noise when the fan stops
running, yet I don't want to call Leica (the manufacturer) because they
charge more money than anyone could ever afford.  How would I go about
finding someone who could repair a cryostat in Pittsburgh? I don't think
it's the refrigeration that's broken, it sounds like something mechanical.
Any suggestions?
Has anyone ever tried to open the back of one to see if it's fixable?
I'm no
mechanic, but I think I can spot a worn belt or loose screw.  I'm just not
sure I want to open it up myself.

Emily

It has become almost a cliche to remark that nobody boasts of ignorance of
literature, but it is socially acceptable to boast ignorance of science and
proudly claim incompetence in mathematics.
-Richard Dawkins
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RE: [Histonet] cryostat repair

[Bouchal, Rena L]

We are using Tech One Biomedical... service reps in Pghfor almost
all of our routine instrument service really good and half the price
of the vendors!  Their number is 866-497-3033 or www.techoneweb.com

Please note that my email address  as of Jan 3, 2011 is
bouch...@wvuhealthcare.com  .   Please make the appropriate changes in
your address book.

Rena Bouchal, M.S.
Anatomic Pathology Manager
West Virginia University Hospitals
304-293-7765

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DANIEL
HEWITT
Sent: Thursday, March 31, 2011 2:15 PM
To: Emily Sours; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat repair

Emily
You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars
and do most of our repairs. Ray does an excellent job at a good price.
It sounds like the bearings in your fan or maybe the compressor.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

This email, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
information.  Any unauthorized review, use, disclosure or distribution
is prohibited.  If you are not the intended recipient, or an agent
responsible for delivering the message to the intended recipient, please
contact the sender by reply e-mail and delete and destroy all copies of
the original message, including attachments.

Please note that any views or opinions presented in this e-mail are
solely those of the author and do not necessarily represent those of
Heritage Valley Health System.  The integrity and security of this
message cannot be guaranteed on the internet.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Thursday, March 31, 2011 1:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat repair

Our cryostat (CM3050S) is making a terrible noise when the fan stops
running, yet I don't want to call Leica (the manufacturer) because they
charge more money than anyone could ever afford.  How would I go about
finding someone who could repair a cryostat in Pittsburgh? I don't think
it's the refrigeration that's broken, it sounds like something
mechanical.
Any suggestions?
Has anyone ever tried to open the back of one to see if it's fixable?
I'm no
mechanic, but I think I can spot a worn belt or loose screw.  I'm just
not
sure I want to open it up myself.

Emily

It has become almost a cliche to remark that nobody boasts of ignorance
of
literature, but it is socially acceptable to boast ignorance of science
and
proudly claim incompetence in mathematics.
-Richard Dawkins
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RE: [Histonet] cryostat repair

[DANIEL HEWITT]

Emily
You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars
and do most of our repairs. Ray does an excellent job at a good price.
It sounds like the bearings in your fan or maybe the compressor.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

This email, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
information.  Any unauthorized review, use, disclosure or distribution
is prohibited.  If you are not the intended recipient, or an agent
responsible for delivering the message to the intended recipient, please
contact the sender by reply e-mail and delete and destroy all copies of
the original message, including attachments.

Please note that any views or opinions presented in this e-mail are
solely those of the author and do not necessarily represent those of
Heritage Valley Health System.  The integrity and security of this
message cannot be guaranteed on the internet.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Thursday, March 31, 2011 1:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat repair

Our cryostat (CM3050S) is making a terrible noise when the fan stops
running, yet I don't want to call Leica (the manufacturer) because they
charge more money than anyone could ever afford.  How would I go about
finding someone who could repair a cryostat in Pittsburgh? I don't think
it's the refrigeration that's broken, it sounds like something
mechanical.
Any suggestions?
Has anyone ever tried to open the back of one to see if it's fixable?
I'm no
mechanic, but I think I can spot a worn belt or loose screw.  I'm just
not
sure I want to open it up myself.

Emily

It has become almost a cliche to remark that nobody boasts of ignorance
of
literature, but it is socially acceptable to boast ignorance of science
and
proudly claim incompetence in mathematics.
-Richard Dawkins
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RE: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)

[Sheila Adey]

Hi Diane,
We just purchased a cryostat from Sakura, we love it. Great features. 
 
> Date: Wed, 30 Mar 2011 06:38:41 -0700
> From: dch...@yahoo.com
> To: diane.glad...@us.army.mil; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
> CC: 
> 
> Hi Diane,
>   We have been happy with the Leica cryostats over the years. We have an 1800 
> that is about 15 years old, an 1850 that is about 5 years old with the UV 
> light 
> for decontamination.
>  Phyllis Thaxton HT(ASCP)QIHC
> DCH Regional Medical Center
> Tuscaloosa, AL 
> 
> 
> 
> 
> 
> From: "Gladney, Diane C Ms CIV USA MEDCOM MACH" 
> To: "histonet@lists.utsouthwestern.edu" 
> Sent: Wed, March 30, 2011 7:50:23 AM
> Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
> 
> Classification:  UNCLASSIFIED
> Caveats: NONE
> 
> Dear Histonetters,
> 
> Our 11 year old cryostat is beginning to experience some unusual problems. 
> Our 
> Medical Maintenance says that they won't put a lot of money into fixing it 
> because of the age of the unit. Therefore, I am asking for opinions, pros, 
> cons 
> of different brands of cryostats. I am interested in a cryostat that has the 
> UV 
> light or other source of easy decontamination. We don't do a lot of frozen 
> sections but enough for us to have to have a good cryostat that is easy to 
> operate and clean.  Any comments or suggestions would be greatly appreciated.
> 
> Thanks,
> Diane Gladney
> 
> 
> Diane C. Gladney, HT (ASCP)
> Histology Supervisor
> Department of Pathology
> Moncrief Army Community Hospital
> 4500 Stuart Street
> FT. Jackson, SC  29207
> 
> Phone:  803-751-2530
> Fax:  803-751-7829
> 
> 
> 
> 
> Classification:  UNCLASSIFIED
> Caveats: NONE
> 
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> 
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Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)

[Phyllis Thaxton]

Hi Diane,
  We have been happy with the Leica cryostats over the years. We have an 1800 
that is about 15 years old, an 1850 that is about 5 years old with the UV light 
for decontamination.
 Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 





From: "Gladney, Diane C Ms CIV USA MEDCOM MACH" 
To: "histonet@lists.utsouthwestern.edu" 
Sent: Wed, March 30, 2011 7:50:23 AM
Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)

Classification:  UNCLASSIFIED
Caveats: NONE

Dear Histonetters,

Our 11 year old cryostat is beginning to experience some unusual problems. Our 
Medical Maintenance says that they won't put a lot of money into fixing it 
because of the age of the unit. Therefore, I am asking for opinions, pros, cons 
of different brands of cryostats. I am interested in a cryostat that has the UV 
light or other source of easy decontamination. We don't do a lot of frozen 
sections but enough for us to have to have a good cryostat that is easy to 
operate and clean.  Any comments or suggestions would be greatly appreciated.

Thanks,
Diane Gladney


Diane C. Gladney, HT (ASCP)
Histology Supervisor
Department of Pathology
Moncrief Army Community Hospital
4500 Stuart Street
FT. Jackson, SC  29207

Phone:  803-751-2530
Fax:  803-751-7829




Classification:  UNCLASSIFIED
Caveats: NONE

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RE: [Histonet] Cryostat help

[Setlak, Lisa]

We have a similar frozen section protocol but we fix the slide in 95% alcohol 
for ~1minute and then stain with Gill's Hematoxylin for 1 minute, rinse, blue 
and then alcohol, foloowed by Eosin for ~ 15secs.- dehydrate and mount.
Lisa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, January 11, 2011 10:29 AM
To: histonet@lists.utsouthwestern.edu; KimberlyBlundon
Subject: Re: [Histonet] Cryostat help

That depends on what staining you need to use.
If an IHC, just fix the sections in acetone, air dry and proceed without HIER
If H&E fix quickly with formalin 10%, wash → hematoxylin for 30 secs without 
differentiation → eosin 15 sec→ dehydrate and mount.
Other HC procedures all will require fixation and using the specific protocol.
Always remember that it is a frozen section and therefore more delicate and 
less adhered than a paraffin section.
René J.

--- On Tue, 1/11/11, Blundon, Kimberly  wrote:


From: Blundon, Kimberly 
Subject: [Histonet] Cryostat help
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, January 11, 2011, 10:28 AM


Hello Histonetters,



I am new to the histology world and I was hoping to get some feedback
about staining after cutting sections in the cryostat. 



Does anyone have a protocol they use for staining after sectioning
tissue in a cryostat?



I have never done it before so I thought I would come to the experts.



Thanks for your help!



Kimberly





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Re: [Histonet] Cryostat help

[Rene J Buesa]

That depends on what staining you need to use.
If an IHC, just fix the sections in acetone, air dry and proceed without HIER
If H&E fix quickly with formalin 10%, wash → hematoxylin for 30 secs without 
differentiation → eosin 15 sec→ dehydrate and mount.
Other HC procedures all will require fixation and using the specific protocol.
Always remember that it is a frozen section and therefore more delicate and 
less adhered than a paraffin section.
René J.

--- On Tue, 1/11/11, Blundon, Kimberly  wrote:


From: Blundon, Kimberly 
Subject: [Histonet] Cryostat help
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, January 11, 2011, 10:28 AM


Hello Histonetters,



I am new to the histology world and I was hoping to get some feedback
about staining after cutting sections in the cryostat. 



Does anyone have a protocol they use for staining after sectioning
tissue in a cryostat?



I have never done it before so I thought I would come to the experts.



Thanks for your help!



Kimberly





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RE: [Histonet] Cryostat Question Please

[Sherwood, Margaret]

We have this model as well and like it.  We got the vacuum attachment.  It's not
perfect, but does facilitate cleaning up OCT and tissue scraps.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Wednesday, November 24, 2010 4:14 PM
To: Paula Lucas; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cryostat Question Please

Very consistant, easy to use. 

2 drawbacks  

1. We got the decontamination add-on trying to automate the CAP
requirement, but now it seems Thermo says it is not an adequate process.
I believe they are doing more intense studies, but for the time being, I
have to decontaminate it by hand. 

2. OCT and tissue scraps build up under collection device and cause the
mechanism to "clunk" unless buildup is removed. 

These two things add a good chunk of time to my day.

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Wednesday, November 24, 2010 1:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Question Please

Hello all
 
Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat?  If so,
what is your opinion/pros and cons?
 
Thanks so much in advance,
Paula
Lab Manager
BP Medical Group
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RE: [Histonet] Cryostat Question Please

[O'Donnell, Bill]

Very consistant, easy to use. 

2 drawbacks  

1. We got the decontamination add-on trying to automate the CAP
requirement, but now it seems Thermo says it is not an adequate process.
I believe they are doing more intense studies, but for the time being, I
have to decontaminate it by hand. 

2. OCT and tissue scraps build up under collection device and cause the
mechanism to "clunk" unless buildup is removed. 

These two things add a good chunk of time to my day.

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Wednesday, November 24, 2010 1:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Question Please

Hello all
 
Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat?  If so,
what is your opinion/pros and cons?
 
Thanks so much in advance,
Paula
Lab Manager
BP Medical Group
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Re: [Histonet] cryostat question

[Alan Bright]

Dear Susan,
Our Starlet 2212 Bench Top Cryostat has an option for orientation. There are 
many used for Mohs, as holding a low temperature is not a problem. I can email 
you some user references  if. You want.
Regards: Alan Bright
Bright Instrument Co. Ltd.
England 
Sent from my BlackBerry® wireless device

-Original Message-
From: histo...@aol.com
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Fri, 29 Oct 2010 22:10:21 
To: 
Subject: [Histonet] cryostat question


Does anyone have 'hands on'  experience performing Mohs using a countertop 
cryostat?  Can the specimen head be manipulated to acccomodate a badly embedded 
block? Does the machine stay cold enough to perform Mohs? Any advice would be 
appreciated.  
My e-mail is histo...@aol.com. Thanks, Susan
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RE: [Histonet] cryostat

[histotech]

Jeff, please note that the URL posted below wrapped into two lines.

Michelle



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histot...@imagesbyhopper.com
Sent: Sunday, August 22, 2010 12:11 PM
To: 'Jeffrey Thompson'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat


Jeff,

We have both the Leica CM1850 and the CM1850 UV. These are not top of the
line models, but they work very well for us.  You can find out more
information on the unit at the following URL:

http://www.leica-microsystems.com/products/histology-systems/sectioning/cryo
stats/details/product/leica-cm1850-uv/


Michelle

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey
Thompson
Sent: Friday, August 20, 2010 5:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat


Hello,
 
I am writing to ask about what the community views are on the best -or worst
- cryostats. We may have the opportunity to purchase one for the first time
in many years and we would like to get a good, solid, reliable model. We're
not really looking for one (and can't really afford one) with all the bells
and whistles, but would like to know what folks think about the ones to look
for as well as the ones to steer clear of.
 
All input is appreciated.
 
Thanks,
 
Jeff Thompson  
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RE: [Histonet] cryostat

[histotech]

Jeff,

We have both the Leica CM1850 and the CM1850 UV. These are not top of the
line models, but they work very well for us.  You can find out more
information on the unit at the following URL:

http://www.leica-microsystems.com/products/histology-systems/sectioning/cryo
stats/details/product/leica-cm1850-uv/


Michelle

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey
Thompson
Sent: Friday, August 20, 2010 5:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat


Hello,
 
I am writing to ask about what the community views are on the best -or worst
- cryostats. We may have the opportunity to purchase one for the first time
in many years and we would like to get a good, solid, reliable model. We're
not really looking for one (and can't really afford one) with all the bells
and whistles, but would like to know what folks think about the ones to look
for as well as the ones to steer clear of.
 
All input is appreciated.
 
Thanks,
 
Jeff Thompson  
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Re: [Histonet] cryostat cutting problems

[Emily Sours]

It may help to let your block equilibrate for about 20 minutes in the
cryostat.  I've found blocks do not section well if you start sectioning
right away, especially snap frozen ones.  Also, do you embed in OCT/sucrose?
A 1:1 OCT: 30% sucrose solution is much softer than just OCT (or whatever
embedding medium you are using).
What do you mean by the tissue is condensed? It's folding up? I've found
sectioning fresh unfixed tissue on a cryostat is impossible (we tried chick
embryo trunks, which may be harder to section fresh than other tissue)--it
doesn't section but get smushed to a pulp.  You need to use a sledge
microtome, unfortunately.
What thickness are you sectioning at? I've found sectioning above sixty
microns doesn't work as well on a cryostat, but again this is on fixed chick
embryo tissue.
If your cryostat is old, I would suggest you look for a new anti-roll plate
made of glass.  They work so much better.  As long as your cryostat isn't
older than about ten years, you should be able to find one that fits your
knife holder very easily.
That said, I've used a Rei-something cryostat that was 15 years old and it
sucked.  The anti-roll plate holder was too loose to do anything useful and
the adjustment of the blade holder was impossible.  Can you use a newer
cryostat in your department from another lab? Or just go back to sledge
microtome sectioning--if you know how to do it, it's the same principle
anyway.

Emily
--
Outside of a dog, a book is man's best friend. Inside of a dog it's too dark
to read.
--Groucho Marx


On Fri, Aug 13, 2010 at 4:53 PM, Entwistle, Laura wrote:

> I am new to using a cryostat and am having some issues with my tissue.
>
>
> 1.Every slice seems to shatter and fragment.  When I have used a
> microtome it was because the tissue was too cold.
>
> 2.   The tissue itself was flash frozen and so was not fixed and the
> tissue did not go through the perfusion process.
>
> 3.   Each slice is condensed and doesn't lay flat.
>
> 4.   The cryostat is old.
>
> Any tips would be very helpful.
> ___
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Re: [Histonet] Cryostat vs decal sections of bone

[Rene J Buesa]

Most definitely there are differences.
Just consider that the bone will not be subjected to any chemicals because 
neither fixation nor demineralization will be required.,
René J.

--- On Mon, 5/10/10, louise renton  wrote:


From: louise renton 
Subject: [Histonet] Cryostat vs decal sections of bone
To: Histonet@lists.utsouthwestern.edu
Date: Monday, May 10, 2010, 4:19 AM


Hi all,

last year sometime I was asked to budget for some equipment for our unit.
Not expecting to get anything i aimed high, and requested a cryostat and
cryoJane system.
Lo and behold, the planetary influences were just right, and my request was
approved. Now, I've got cold feet (pardon the pun) and I'm wondering if
there *are*  any distinct advantages of cryosections of bone over
demineralised wax embedded samples in regards to immuno and in-situ ?.

-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
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RE: [Histonet] cryostat help please!

[Alan Bright]

Dear Mohamed,

We manufacture cryostats but not the one you have the problem with, however I 
think what you can hear is the cooling fan working but not the refrigeration 
compressor. 
You will need to locate the internal fuses to see which ones need replacing. If 
the compressor is running then it would seem to have lost its refrigeration 
gas. Unfortunately I am leaving my office now and Histonet does not seem to 
like my Blackberry, but if you would like to talk further on this problem you 
can catch me on Skype dazzle0 later today or let me have your phone number and 
I will call you.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abri...@brightinstruments.com
Web Site: www.brightinstruments.com
Skype: dazzle0


 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el 
razik
Sent: 20 March 2010 13:31
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat help please!

hi all histoneters 
i have learned alot from your posts and i need any suggestion about a cryostat 
that was
working perfectly until the power cable is pluged off accidently. then after 
about 2 days i put it again in the power and all is working with its motor 
sound but no refregration and room temp. not decrease under 20 degrees!!! i let 
it for aweek but no change!  unfortene the technical service may take long time 
till it come to see the proplem as it is in a national university ( routine 
work) and i hope i could do that. any suggestion please?



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RE: [Histonet] cryostat help please!

[R J VAZQUEZ]

I agree with Joyce, it sounds like the fuse for the refrigeration.  I had that 
problem a few times.

Robyn Vazquez
 
> Date: Sun, 21 Mar 2010 08:28:36 -0400
> From: jwe...@sjha.org
> To: k8...@yahoo.com; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] cryostat help please!
> CC: 
> 
> Sounds like it may be a fuse... in my non-expert opinion!! j
> 
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 678-843-7376 - Phone
> 678-843-7831 - Fax
> 
> 
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu on behalf of mohamed abd el 
> razik
> Sent: Sat 3/20/2010 9:30 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] cryostat help please!
> 
> hi all histoneters 
> i have learned alot from your posts and i need any suggestion about a 
> cryostat that was
> working perfectly until the power cable is pluged off accidently. then after 
> about 2 days i put it again in the power and all is working with its motor 
> sound but no refregration and room temp. not decrease under 20 degrees!!! i 
> let it for aweek but no change!  unfortene the technical service may take 
> long time till it come to see the proplem as it is in a national university ( 
> routine work) and i hope i could do that. any suggestion please?
> 
> 
> 
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> This email, including any attachments is the 
> property of Catholic Health East and is intended 
> for the sole use of the intended recipient(s). 
> It may contain information that is privileged and 
> confidential. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are 
> not the intended recipient, please reply to the 
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Re: [Histonet] cryostat help please!

[mohamed abd el razik]

our cryostat is 
Minotome Plus 
Microtome Cryostat
Cat. No. 2563-- For 120 VAC, 60Hz
 

--- On Sat, 3/20/10, Cheryl Cornett-Early  wrote:


From: Cheryl Cornett-Early 
Subject: Re: [Histonet] cryostat help please!
To: "mohamed abd el razik" 
Date: Saturday, March 20, 2010, 10:59 PM







Hi Mohamed,
 I AM a Services guy and this problem sounds like a loss of 
refrigerant I come across this problem on older Cryostat's after they have been 
turned off regularly, What  model of cryostat is it?. IT sounds like a CM1800 
Riechet Jung or (Leica).
Best regards
Brian.
My territory is the south east, GA, AL, TN, SC, NC,

--- On Sat, 3/20/10, mohamed abd el razik  wrote:


From: mohamed abd el razik 
Subject: [Histonet] cryostat help please!
To: histonet@lists.utsouthwestern.edu
Date: Saturday, March 20, 2010, 9:30 AM


hi all histoneters 
i have learned alot from your posts and i need any suggestion about a cryostat 
that was
working perfectly until the power cable is pluged off accidently. then after 
about 2 days i put it again in the power and all is working with its motor 
sound but no refregration and room temp. not decrease under 20 degrees!!! i let 
it for aweek but no change!  unfortene the technical service may take long time 
till it come to see the proplem as it is in a national university ( routine 
work) and i hope i could do that. any suggestion please?

Histonet@lists.utsouthwestern.edu


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RE: [Histonet] cryostat help please!

[Weems, Joyce]

Sounds like it may be a fuse... in my non-expert opinion!! j

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu on behalf of mohamed abd el 
razik
Sent: Sat 3/20/2010 9:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat help please!
 
hi all histoneters 
i have learned alot from your posts and i need any suggestion about a cryostat 
that was
working perfectly until the power cable is pluged off accidently. then after 
about 2 days i put it again in the power and all is working with its motor 
sound but no refregration and room temp. not decrease under 20 degrees!!! i let 
it for aweek but no change!  unfortene the technical service may take long time 
till it come to see the proplem as it is in a national university ( routine 
work) and i hope i could do that. any suggestion please?


  
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Re: [Histonet] cryostat help please!

[mohamed abd el razik]

thanks for your replay ray
yes i set the temp. to -20 degrees but it is 20 for the last week. 
--- On Sat, 3/20/10, Mark Ray  wrote:


From: Mark Ray 
Subject: Re: [Histonet] cryostat help please!
To: "mohamed abd el razik" 
Date: Saturday, March 20, 2010, 5:03 PM


Are you sure the temperature control is set properly?  This maybe a serious 
problem, but it is possible that the people who do the ordinary repairs on 
refrigeration equipment for the university can repair it.  You should ask them 
if they can help you.

mohamed abd el razik wrote:
> hi all histoneters i have learned alot from your posts and i need any 
> suggestion about a cryostat that was
> working perfectly until the power cable is pluged off accidently. then after 
> about 2 days i put it again in the power and all is working with its motor 
> sound but no refregration and room temp. not decrease under 20 degrees!!! i 
> let it for aweek but no change!  unfortene the technical service may take 
> long time till it come to see the proplem as it is in a national university ( 
> routine work) and i hope i could do that. any suggestion please?
> 
> 
>       ___
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>   





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RE: [Histonet] Cryostat better than others?

[Rathborne, Toni]

We currently have a Leica and a Jung. They are serviced twice a year. Prior to 
these, we had two Tissue Teks that were donated to us. My best guess is that 
they were about 20 years old when they were replaced.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of
Fairbairn, Patricia
Sent: Thursday, March 04, 2010 2:59 PM
To: Histonet
Subject: [Histonet] Cryostat better than others?


Hi All,
 
Are there any cryostat makes that seem to be more reliable than others? We have 
not been having much luck with our particular Microms after moving them 
cross-country, getting recalled, catching on fire, corroding, etc. While one 
machine serves as an excellent calendar stand (with padded resting pads and 
everything!) and our other one just went down at the ripe age of 2, I would 
rather purchase machines that work without costing us so much in repairs. ...or 
are we just jinxed?
 
Also, how long do cryostats tend to last? What do you think of service 
contracts? I keep hearing that they are pretty much worthless and that the 
cryostat "should" be fine if it's served once a year.
 
Thank you!
 
PJ Fairbairn
Staff Research Associate
Department of Neurological Surgery
Brain and Spinal Injury Center
University of California at San Francisco

fairbai...@neurosurg.ucsf.edu   


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Re: [Histonet] Cryostat decon- what a pain in the butt

[Pamela Marcum]

Jeff, 



Can you give the reference for the CAP "adjustment" on decon for crystats 
please?  We have two working cryostats and average 6 to 10 cases (not 
specimens) a day.  This would effectively shut us down for frozens on some 
days.  We have third one that is currently in for repair. 



Thanks, 

Pam Marcum 

UAMS 

Anatomic Patholgoy Manager 





- Original Message - 
From: "Tim Morken"  
To: "Jeffrey Silverman" , "o isaac24" 
 
Cc: histonet@lists.utsouthwestern.edu 
Sent: Monday, February 22, 2010 10:23:17 AM GMT -06:00 US/Canada Central 
Subject: RE: [Histonet] Cryostat decon- what a pain in the butt 

Jeff, Do they give any references for the effectiveness of their proposed 
method? 


Tim Morken 
Supervisor, Histology / IPOX 
UCSF Medical Center 
San Francisco, CA   
  
-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey 
Silverman 
Sent: Sunday, February 21, 2010 12:20 PM 
To: o.isaa...@yahoo.com 
Cc: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Cryostat decon- what a pain in the butt 

CAP is moving to more rigorous cryostat decontamnation methods - mandating a 
weekly shutdown and wet chemical disinfection with a tuberculocidal agent for 
machines used regularly. Now, if a lab is doing 1-3 frozens a week, is that 
used regularly? We must lobby the CAP for more sensible and practical 
guidlines. The old wipe down with 70% ETOH without bringing the machine will 
become non-compliant and useful only as an interim measure.   
By the way, the UV lamps do not satisfy the CAP standard, I believe. 
  
Our system has gone to a commercially available tuberculocidal, virucidal, and 
broad spectrum bacteriocidal moistened wipes the name of which I will post 
tomorrow when I get to the job. 
  
Here is a skeletonized basic procedure form what CAP will require: 
  
1: Remove all utensils and brush out and collect section debris disposing of 
this according to regulated medical waste protocol (red bag). 
2. Bring the instrument to room temperature. 
3: Wipe all working surfaces with the tuberculocidal wipes, visibly moistening 
all surfaces. Surface must remain wet for 2 minutes. Use multiple wipes as 
needed. Instruments can be similarly disinfected. 
4: Carefully dry all surfaces with gauze. Dispose of all wipes and gauze as 
biohazardous. 
5: Dry and lubricate the microtome as per manufacturer's instructions. 
6. Turn on crystat and bring to working temperature. 
7. Document procedure on your maintenance log. 
8. Look forward to doing it again next week. 
  
  
Look, this is a necessary procedure, but weekly??? Perhaps some workload- based 
formula or an alert system- like pathologists alert the lab when  a case with 
granulomas or caseating necrosis is sectioned.  Every lab will have to bring a 
tech in on weekends or at night, to do this, or have two cryostats to 
compensate for the fully one working day most machines will have to be down to 
be cleaned in this manner. 
  
Thoughts? 
  
Jeff Silverman 
  
  
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RE: [Histonet] Cryostat decon- what a pain in the butt

[Morken, Tim]

Jeff, Do they give any references for the effectiveness of their proposed 
method? 


Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey 
Silverman
Sent: Sunday, February 21, 2010 12:20 PM
To: o.isaa...@yahoo.com
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat decon- what a pain in the butt

CAP is moving to more rigorous cryostat decontamnation methods - mandating a 
weekly shutdown and wet chemical disinfection with a tuberculocidal agent for 
machines used regularly. Now, if a lab is doing 1-3 frozens a week, is that 
used regularly? We must lobby the CAP for more sensible and practical 
guidlines. The old wipe down with 70% ETOH without bringing the machine will 
become non-compliant and useful only as an interim measure.  
By the way, the UV lamps do not satisfy the CAP standard, I believe. 
 
Our system has gone to a commercially available tuberculocidal, virucidal, and 
broad spectrum bacteriocidal moistened wipes the name of which I will post 
tomorrow when I get to the job. 
 
Here is a skeletonized basic procedure form what CAP will require:
 
1: Remove all utensils and brush out and collect section debris disposing of 
this according to regulated medical waste protocol (red bag). 
2. Bring the instrument to room temperature.
3: Wipe all working surfaces with the tuberculocidal wipes, visibly moistening 
all surfaces. Surface must remain wet for 2 minutes. Use multiple wipes as 
needed. Instruments can be similarly disinfected. 
4: Carefully dry all surfaces with gauze. Dispose of all wipes and gauze as 
biohazardous. 
5: Dry and lubricate the microtome as per manufacturer's instructions. 
6. Turn on crystat and bring to working temperature. 
7. Document procedure on your maintenance log. 
8. Look forward to doing it again next week. 
 
 
Look, this is a necessary procedure, but weekly??? Perhaps some workload- based 
formula or an alert system- like pathologists alert the lab when  a case with 
granulomas or caseating necrosis is sectioned.  Every lab will have to bring a 
tech in on weekends or at night, to do this, or have two cryostats to 
compensate for the fully one working day most machines will have to be down to 
be cleaned in this manner. 
 
Thoughts?
 
Jeff Silverman
 
 
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RE: [Histonet] Cryostat Poll

[Maria Katleba]

We have a LEICA CRYOSTAT... its EXCELLENT!

Yes- we have a contract and NO never had issues...

Maria Katleba  HT(ASCP) MS
Queen of the Valley Medical Center
Napa CA 94558



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham
Sent: Tuesday, December 01, 2009 2:22 PM
To: Merced M Leiker
Cc: Histonet
Subject: Re: [Histonet] Cryostat Poll

yes and yes





Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097

"happy slicing and dicing and may all your stains work perfectly" -
Paula Sicurello
P Please consider the environment before printing this email.




On Dec 1, 2009, at 12:12 PM, Merced M Leiker wrote:

> For Researchers Only who have cryostats (1-2 hrs/wk ave usage):
>
> 1. Is your cryostat under service contract?
>
> 2. Have you had any problems with your cryostat that would justify
> having a service contract?
>
> Thank you!
>
> Merced M Leiker
> Research Technician III
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214  USA
> lei...@buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
>
> No trees were harmed in the sending of this email.
> However, many electrons were severely inconvenienced.
>
>
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Re: [Histonet] Cryostat Poll

[Andrea Grantham]

yes and yes





Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097

"happy slicing and dicing and may all your stains work perfectly" -  
Paula Sicurello

P Please consider the environment before printing this email.




On Dec 1, 2009, at 12:12 PM, Merced M Leiker wrote:


For Researchers Only who have cryostats (1-2 hrs/wk ave usage):

1. Is your cryostat under service contract?

2. Have you had any problems with your cryostat that would justify  
having a service contract?


Thank you!

Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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RE: [Histonet] Cryostat decontamination...

[Liz Chlipala]

>From what I understand if you require that an individual wear a N95
respirator when they are sectioning frozen sections then you are also
required to have that respirator fit tested yearly.  

We are a research lab that does quite a bit of work on TB samples.  We
do not section TB infected samples on a cryostat.  All samples that we
receive have been fixed in 10% NBF for several days and then transferred
to 70% alcohol.  When I was in a clinical lab, we would not section
frozen sections of lung samples if they wanted to rule out TB, the
pathologist would recommend to process to paraffin first.  In fact since
the samples are generated in a biosafety level 2 or 3 facility, they can
not leave that facility unless the samples have been fixed and rendered
non infectious.  Frozen sections would need to be prepared within that
biosafety facility with all of the appropriate PPE's in place.  The
University that we work with has tested these samples via culture after
their fixation and alcohol procedure.  We do however offer the N95
respirator to the techs and they can wear it if they want to, when they
are grossing, embedding or sectioning these samples.  But since it is
voluntary we do not have to fit test, we have a specific procedure that
covers this.  This protocol has been developed with the help of our
local OSHA rep.  The other thing is we use a special vacumme with a hepa
filter to vacume up the paraffin trimmings.  OSHA has a program for
small businesses and will work with them to make sure that they are
within compliance, they have been very helpful to us here.  Just go to
the OSHA website.   

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom
McNemar
Sent: Monday, November 16, 2009 12:17 PM
To: histo...@pathology.swmed.edu
Subject: [Histonet] Cryostat decontamination...

Hello all,

We have always used absolute alcohol to decontaminate our cryostat and
wondered what others use.  This has been brought up by an article in the
September issue of CAP Today that talks about biosafety when doing
frozens (specifically talks about TB).  As a related question, how many
institutions require the wearing on an N95 respirator when doing
frozens?

The article states that diluted alcohol is more effective "because the
presence of water causes proteins to denature more quickly."  I just did
the decontamination of our cryostat last weekend and used 70% alcohol as
the article suggested.  The alcohol evaporated and I was left with
beads/drops of water that I had to then dry by hand.  I guess the
alternative would be is just to go over it again with aboslute after
usiing 70%.

The article goes on to recommend that personnel should wear N95 masks
that are fit tested on a yearly basis.

Thanks in advance.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


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Re: Re: [Histonet] Cryostat sections mounting with 0.1M PB solution onto slides

[TF]

How about to use a drop of water?

Sometimes the section is a bit big, and it is hard to mount the section very 
flat on slides without a bubble...

2009-04-20 



TF 



发件人： Paula Pierce 
发送时间： 2009-04-03  23:59:43 
收件人： tifei 
抄送： 
主题： Re: [Histonet] Cryostat sections mounting with 0.1M PB solution onto slides 
 
Hi TF,

you should not place the drop of buffer on the slide under the section. The 
section will wash off. You will get buffer crystals under the section during 
drying and although they will dissolve out later, they will cause the section 
to not stick to the slide where they are.

Place the section directly on a warm (room temperature) slide and the section 
will adhere.

Paula





From: TF 
To: Histonet 
Sent: Friday, April 3, 2009 10:43:03 AM
Subject: [Histonet] Cryostat sections mounting with 0.1M PB solution onto slides

After we cut the brain sections on a cryostat (OCT embedded), we brush a drop 
of 0.1M PB on to the slide before mounting the sections.
Do anyone know the side effect of this - for example, the sections will peel 
off during immunostaining?

Another question is about the dry time after mounting and before staining.
I know some people asked similar questions before, but they are using fresh 
tissue for frozen sections.
We here perfuse the animal, post-fix the tissue and sink it in sucrose before 
making cryostat sections.
So we may dry the sections in air up to weeks.
I would like to ask about the proper heating time (with a heat plate) before 
dry the sections up in a fumehood.
Should we heat the sections at 60 degree for 10 min or 2 hours shortly after 
mounting?

Thanks very much.


2009-04-03 



TF 
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RE: [Histonet] cryostat adapter

[YourBiomed.Com]

We have the adapter here at IMEB, Inc.
Please go to www.imebinc.com for 
our contact info and ask for Brad.

Thank  you

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RE: [Histonet] Cryostat safety question

[Helen Fedor]

Hello,  I had previously sent this but only to the person who asked the 
question, I think it this works beautifully so decided to resend to the list.

we have a great way to remove the specimens for the chucks. We have a 500cc 
plastic container with a lid that we have cut an "X" into the center.  We put 
warm water in the container and then just put the chuck stem into the "X". 
Within 10 seconds the block can be removed. Still frozen.  We just keep the 
plastic container at the bench and keep reusing the same one. The water level 
does need to come all the way up to the lid, But the chuck never gets wet. We 
do the sealing of the tissue with a tiny amount of OCT to the surface of the 
still frozen block, while it is still in the cryostat as well.


Helen

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Wednesday, January 07, 2009 7:38 PM
To: Histonet
Cc: mari.ann.mailh...@leica-microsystems.com
Subject: RE: [Histonet] Cryostat safety question

Andrea:
I work in a Mohs clinic where all we cut is frozen skin sections. Needless to 
say, we don't have 50 chucks laying around... In the morning before clinic 
starts we put a layer of freezing medium on chucks and put them in the cryostat 
to freeze. When we get specimens, we add another drop or so to the already 
frozen 'button' and immediately embed the tissue in it. We usually add another 
small drop on top after it has begun to freeze, to cover the specimen 
completely. Cut as normal when frozen. After done cutting all you have to do is 
use a forceps or other blunt object and pop the bit with the specimen in it 
away from the 'button' and return the chuck to the cryostat and it can be 
reused the rest of the day. The specimen is therefore still frozen for storage, 
and it has a quicker TAT. Plus you won't need nearly so many chucks, as they 
can be recycled almost as soon as you are done cutting. I usually keep 6-8 
'buttons' in my cryostat, and our clinic can process up to 50 separate 
specimens a day. A word of caution. If your work area is humid sometimes a thin 
layer of frost can form on the surface of the 'button' and when you attempt to 
take sections the bit with the tissue will pop off the 'button'. All you need 
to do is add another drop of medium to the button and 'glue' the two back 
together. If you are going a while between cutting sessions, I usually store my 
'buttons' upside(mountant side) down on one of the cryostat surfaces. It 
doesn't seem to develop the frost layer. Useful if you have tiny specimens.
Hope my verbose explanation is helpful. Feel free to e-mail if you have any 
questions or are confused about my explanation.

Claire Ingles
Madison WI



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Andrea Hooper
Sent: Wed 1/7/2009 5:40 PM
To: Histonet
Cc: mari.ann.mailh...@leica-microsystems.com
Subject: [Histonet] Cryostat safety question



The discussion on microtome safety begs me to ask a cryostat question 

We have a Leica CM3050 cryostat and love it!

How are people (and perhaps only those in research do this) removing
their tissue from the chucks for future use? We often just section a
few slides worth then put the block at -80 deg C for future studies.
Needless to say, it's the most dangerous part of our day.

So what are your suggestions for removing tissue from a chuck (and
melting it isn't really a viable option)?

Thanks in advance,
Andrea
--

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Re: [Histonet] Cryostat safety question

[anh2006]

That sounds very cool for the lucky owners of one of your cryostats, esp the 
part about orientation ... but nearly every block of ours goes back in the 
freezer so I think it would take up too much space and end up being expensive, 
no (we are talking 100s and 100s of block)?

--Original Message--
From: Alan Bright
To: Andrea Hooper
To: Histonet
Sent: Jan 8, 2009 7:06 AM
Subject: RE: [Histonet] Cryostat safety question

Andrea,

For users of our cryostats that need to use the specimen for future use
we manufacture  disposable object holders & quick release holders that
fits specimen containers for LN2 or -80 deg. C storage. The tissue stays
on the disposable object holders during storage too and has an index
location so that it only goes back onto the cryostat microtome in the
correct orientation. No sorry but it will not be suitable for your
cryostat or other Chinese/ German manufactured types.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abri...@brightinstruments.com
Web Site: www.brightinstruments.com
Skype: dazzle0




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea
Hooper
Sent: 07 January 2009 23:40
To: Histonet
Cc: mari.ann.mailh...@leica-microsystems.com
Subject: [Histonet] Cryostat safety question

The discussion on microtome safety begs me to ask a cryostat question


We have a Leica CM3050 cryostat and love it!

How are people (and perhaps only those in research do this) removing 
their tissue from the chucks for future use? We often just section a 
few slides worth then put the block at -80 deg C for future studies. 
Needless to say, it's the most dangerous part of our day.

So what are your suggestions for removing tissue from a chuck (and 
melting it isn't really a viable option)?

Thanks in advance,
Andrea
-- 

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Re: [Histonet] Cryostat safety question

[anh2006]

Very ingenious technique Claire! 

However, I would hesitate to use this with our particular cryostat as I find 
when doing this type of technique (keeping a button), or using any chucks which 
are not absolutely RT I get thick-thin sections as the tissue isn't super 
firmly adhered to the chucks. If I use super clean, room temp chucks my 
sections are 100% better quality. In fact when teaching users to use the 
cryostat I always make a big deal about this issue. The thick-thin issue seems 
to be improved somewhat since Leica revamped their chucks and now have much 
bigger grooves - so I am convinced firm adherance is key to quality sections.

I would be curious if anyone had any opinions on this, or experienced the same 
thing (thick thin sections as a result of chuck/tissue adherance). If Leica 
peops want to chime in about it too, I would welcome that.

Andrea


-Original Message-
From: Ingles Claire 

Date: Wed, 07 Jan 2009 18:38:04 
To: Histonet
Cc: 
Subject: RE: [Histonet] Cryostat safety question


Andrea:
I work in a Mohs clinic where all we cut is frozen skin sections. Needless to 
say, we don't have 50 chucks laying around... In the morning before clinic 
starts we put a layer of freezing medium on chucks and put them in the cryostat 
to freeze. When we get specimens, we add another drop or so to the already 
frozen 'button' and immediately embed the tissue in it. We usually add another 
small drop on top after it has begun to freeze, to cover the specimen 
completely. Cut as normal when frozen. After done cutting all you have to do is 
use a forceps or other blunt object and pop the bit with the specimen in it 
away from the 'button' and return the chuck to the cryostat and it can be 
reused the rest of the day. The specimen is therefore still frozen for storage, 
and it has a quicker TAT. Plus you won't need nearly so many chucks, as they 
can be recycled almost as soon as you are done cutting. I usually keep 6-8 
'buttons' in my cryostat, and our clinic can process up to 50 separate 
specimens a day. A word of caution. If your work area is humid sometimes a thin 
layer of frost can form on the surface of the 'button' and when you attempt to 
take sections the bit with the tissue will pop off the 'button'. All you need 
to do is add another drop of medium to the button and 'glue' the two back 
together. If you are going a while between cutting sessions, I usually store my 
'buttons' upside(mountant side) down on one of the cryostat surfaces. It 
doesn't seem to develop the frost layer. Useful if you have tiny specimens.
Hope my verbose explanation is helpful. Feel free to e-mail if you have any 
questions or are confused about my explanation. 
 
Claire Ingles
Madison WI



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Andrea Hooper
Sent: Wed 1/7/2009 5:40 PM
To: Histonet
Cc: mari.ann.mailh...@leica-microsystems.com
Subject: [Histonet] Cryostat safety question



The discussion on microtome safety begs me to ask a cryostat question 

We have a Leica CM3050 cryostat and love it!

How are people (and perhaps only those in research do this) removing
their tissue from the chucks for future use? We often just section a
few slides worth then put the block at -80 deg C for future studies.
Needless to say, it's the most dangerous part of our day.

So what are your suggestions for removing tissue from a chuck (and
melting it isn't really a viable option)?

Thanks in advance,
Andrea
--

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RE: [Histonet] Cryostat safety question

[Alan Bright]

Andrea,

For users of our cryostats that need to use the specimen for future use
we manufacture  disposable object holders & quick release holders that
fits specimen containers for LN2 or -80 deg. C storage. The tissue stays
on the disposable object holders during storage too and has an index
location so that it only goes back onto the cryostat microtome in the
correct orientation. No sorry but it will not be suitable for your
cryostat or other Chinese/ German manufactured types.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abri...@brightinstruments.com
Web Site: www.brightinstruments.com
Skype: dazzle0




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea
Hooper
Sent: 07 January 2009 23:40
To: Histonet
Cc: mari.ann.mailh...@leica-microsystems.com
Subject: [Histonet] Cryostat safety question

The discussion on microtome safety begs me to ask a cryostat question


We have a Leica CM3050 cryostat and love it!

How are people (and perhaps only those in research do this) removing 
their tissue from the chucks for future use? We often just section a 
few slides worth then put the block at -80 deg C for future studies. 
Needless to say, it's the most dangerous part of our day.

So what are your suggestions for removing tissue from a chuck (and 
melting it isn't really a viable option)?

Thanks in advance,
Andrea
-- 

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Re: [Histonet] Cryostat safety question

[Beth Millerman]

I have the same cyostat, but it doesn't matter which one you use for
removing tissue from the chuck is a separate issue.  Others may beg to
differ from my technic, but I remove the block from the cryostat and set it
on a paper towel for a couple of mintes. I do not thaw tissue, just soften
it. I take a scapel or small weigh spatula and gently separate it off the
chuck. With a thin layer of OCT in the plastic mold, I return the block
back to its original labeled mold, wrap with aluminum foil, place in a
ziploc, and store it back to the -80C. This is quick and limits exposure to
higher temps.

I have used blocks multiple times, especially for cross reactivity studies
and never had a problem. I am very careful and need reusing the block for
IHC receptor studies. Good luck. This is pretty easy and you can move on to
the next block at the same time.


Beth Millerman/SWC
Senior Research Associate
Stiefel Laboratories, Inc



   
   "Andrea Hooper"
   Sent by: histonet-boun...@lists.utsouthwestern.edu  
   
   Wed 07 Jan 2009 03:40 PM
   --- 
   
   
   
To 
   
 Histonet  
   
cc 
 mari.ann. 
 mailhiot@ 
 leica-mic 
 rosystems 
 .com  
   Subject 
 [Histonet 
 ] 
 Cryostat  
 safety
 question  
   
   
   
   
   
   --- 




The discussion on microtome safety begs me to ask a cryostat question 

We have a Leica CM3050 cryostat and love it!

How are people (and perhaps only those in research do this) removing
their tissue from the chucks for future use? We often just section a
few slides worth then put the block at -80 deg C for future studies.
Needless to say, it's the most dangerous part of our day.

So what are your suggestions for removing tissue from a chuck (and
melting it isn't really a viable option)?

Thanks in advance,
Andrea
--

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RE: [Histonet] Cryostat safety question

[Ingles Claire]

Andrea:
I work in a Mohs clinic where all we cut is frozen skin sections. Needless to 
say, we don't have 50 chucks laying around... In the morning before clinic 
starts we put a layer of freezing medium on chucks and put them in the cryostat 
to freeze. When we get specimens, we add another drop or so to the already 
frozen 'button' and immediately embed the tissue in it. We usually add another 
small drop on top after it has begun to freeze, to cover the specimen 
completely. Cut as normal when frozen. After done cutting all you have to do is 
use a forceps or other blunt object and pop the bit with the specimen in it 
away from the 'button' and return the chuck to the cryostat and it can be 
reused the rest of the day. The specimen is therefore still frozen for storage, 
and it has a quicker TAT. Plus you won't need nearly so many chucks, as they 
can be recycled almost as soon as you are done cutting. I usually keep 6-8 
'buttons' in my cryostat, and our clinic can process up to 50 separate 
specimens a day. A word of caution. If your work area is humid sometimes a thin 
layer of frost can form on the surface of the 'button' and when you attempt to 
take sections the bit with the tissue will pop off the 'button'. All you need 
to do is add another drop of medium to the button and 'glue' the two back 
together. If you are going a while between cutting sessions, I usually store my 
'buttons' upside(mountant side) down on one of the cryostat surfaces. It 
doesn't seem to develop the frost layer. Useful if you have tiny specimens.
Hope my verbose explanation is helpful. Feel free to e-mail if you have any 
questions or are confused about my explanation. 
 
Claire Ingles
Madison WI



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Andrea Hooper
Sent: Wed 1/7/2009 5:40 PM
To: Histonet
Cc: mari.ann.mailh...@leica-microsystems.com
Subject: [Histonet] Cryostat safety question



The discussion on microtome safety begs me to ask a cryostat question 

We have a Leica CM3050 cryostat and love it!

How are people (and perhaps only those in research do this) removing
their tissue from the chucks for future use? We often just section a
few slides worth then put the block at -80 deg C for future studies.
Needless to say, it's the most dangerous part of our day.

So what are your suggestions for removing tissue from a chuck (and
melting it isn't really a viable option)?

Thanks in advance,
Andrea
--

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Re: [Histonet] Cryostat safety question

[Jan . Minshew]

Hi Andrea,

It's great to hear that you are happy with your cryostat and, hopefully,
I'll be able to help you with an answer to your question.  Leica sells a
small device called a Thermal Block that is designed to help you remove the
specimen from the chuck without causing it to melt.  The catalog number is
14039818542.  It's a small, mobile device that can be used in any of our
cryostats that use chucks with stems in the back (we have a different one
for the new CM1950 flat backed chucks).
If you have questions or would like additional information, please feel
free to contact our Technical Applications Center (1-800-248-0123 option 1
then option 2).

Best wishes,


Jan Minshew, HT/HTL(ASCP)
Marketing Manager
Leica Microsystems
Biosystems Division
2345 Waukegan Road
Bannockburn, IL 60015

800.248.0123 Toll Free
847.405.7051 Direct
847.405.6560 Fax

www.leica-microsystems.com

Click Here for this month's special offers!


   
 "Andrea Hooper"   
To 
 Sent by:  Histonet
 histonet-bounces@  
 lists.utsouthwest  cc 
 ern.edu   mari.ann.mailh...@leica-microsystem 
   s.com   
   Subject 
 01/07/2009 05:40  [Histonet] Cryostat safety question 
 PM
   
   
   
   
   




The discussion on microtome safety begs me to ask a cryostat question 

We have a Leica CM3050 cryostat and love it!

How are people (and perhaps only those in research do this) removing
their tissue from the chucks for future use? We often just section a
few slides worth then put the block at -80 deg C for future studies.
Needless to say, it's the most dangerous part of our day.

So what are your suggestions for removing tissue from a chuck (and
melting it isn't really a viable option)?

Thanks in advance,
Andrea
--

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RE: [Histonet] Cryostat- information for new please

[William Connor]

The Microm HM 550 is an awesome cryostat, and meets requirements 1-3 for
sure.  Not sure about the price.  I think we got ours used...  There is room
for a 100 count slide box, but you'd have to maneuver it a bit in order to
get the lid open and a slide in there.

William Connor

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of
[EMAIL PROTECTED]
Sent: Friday, November 14, 2008 9:44 AM
To: Histonet
Subject: [Histonet] Cryostat- information for new please

Hi All, including Vendors
We're in the market for a new cryostat.  Here are our needs:

1. Cutting rat and mouse brains.
2. Ability to keep temperature while cutting for a couple hours at a time.
3. Able to cut 8 to 20 microns.
4. Room in the cryostat for a 100 slide holder (to keep sections frozen 
after cutting.
5. Good price, as this is research.  We don't need anything fancy.

Thanks for any information you can give,
Cindi Roberts
HFH
Neurology Research



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Re: [Histonet] cryostat SOP

[Rene J Buesa]

As part of the general SOP, under sectioning.
René J.

--- On Tue, 10/28/08, Michele Wich <[EMAIL PROTECTED]> wrote:

From: Michele Wich <[EMAIL PROTECTED]>
Subject: [Histonet] cryostat SOP
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 28, 2008, 3:46 PM

An SOP question for those out there who are forced to write these
tedious things: Do you maintain a separate cryostat SOP, or is it
something that is typically included in a general microtome SOP? Any
help with this would be greatly appreciated!


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RE: [Histonet] cryostat blues

[Terri Brown]

Good going  Only us OLD TECH know this.

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Weems,
Joyce
Sent: Wednesday, October 15, 2008 9:29 AM
To: Emily Sours; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat blues

Those who are full of hot air can also blow gently and will generate
enough humidity to reduce the static. J:>)

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
Please note new phone and fax numbers
678-843-7376 - Phone
678-843-7831 - Fax



-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Emily
Sours
Sent: Wednesday, October 15, 2008 8:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] cryostat blues

Unfortunately, this sounds like static electricity.
Leica suggests spraying anti-static spray in the chamber (!).  You could
also ground the metal in the chamber using metal wire touching something
metal outside the chamber (the Russian post-doc solution, very clever).
We keep 100% EtOH in a small container (about 100 ml) in the back of the
chamber to reduce static, which I learned from a long ago histonet post,
so I have no idea why it works.
Otherwise, buy an anti-static brush, which I hear exists, but have never
seen.

Emily
--
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Re: [Histonet] cryostat blues

[Jan . Minshew]

Hello Emily,

I'm so sorry if someone told you to spray an anti-static spray into the
cryostat chamber.  That is not a recommended practice and it should not be
done.  Spraying anything into a cryochamber creates an aerosol that can
contaminate the air in the breathing zone of the operator...and we all know
what is lurking in the depths of our cryostats.

As you suggested, storing a small container of alcohol in the back of the
cryostat while sectioning (remove when finished) and grounding the blade
holder are effective and safe ways to reduce static electricity.  You can
also try placing an anti-static clothes dryer sheet in front of or behind
the knife holder.

Again, I apologize for the misinformation.  Please feel free to contact me
if I can help in any other way.

Best wishes,


Jan Minshew, HT/HTL(ASCP)
Marketing Manager
Leica Microsystems
Biosystems Division
2345 Waukegan Road
Bannockburn, IL 60015

800.248.0123 Toll Free
847.405.7051 Direct
847.405.6560 Fax

www.leica-microsystems.com

Click Here for this month's special offers!


   
 "Emily Sours" 
 <[EMAIL PROTECTED] 
 l.com> To 
 Sent by:  histonet@lists.utsouthwestern.edu   
 histonet-bounces@  cc 
 lists.utsouthwest 
 ern.edu   Subject 
           Re: [Histonet] cryostat blues   
   
 10/15/2008 07:54  
 AM
   
   
   




Unfortunately, this sounds like static electricity.
Leica suggests spraying anti-static spray in the chamber (!).  You could
also ground the metal in the chamber using metal wire touching something
metal outside the chamber (the Russian post-doc solution, very clever).  We
keep 100% EtOH in a small container (about 100 ml) in the back of the
chamber to reduce static, which I learned from a long ago histonet post, so
I have no idea why it works.
Otherwise, buy an anti-static brush, which I hear exists, but have never
seen.

Emily
--
the velocity of time turns her voice into sugar water
http://www.youtube.com/watch?v=jNA6zzoObxg
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RE: [Histonet] cryostat blues

[Weems, Joyce]

Those who are full of hot air can also blow gently and will generate
enough humidity to reduce the static. J:>)

Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
Please note new phone and fax numbers
678-843-7376 - Phone
678-843-7831 - Fax



-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Emily
Sours
Sent: Wednesday, October 15, 2008 8:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] cryostat blues

Unfortunately, this sounds like static electricity.
Leica suggests spraying anti-static spray in the chamber (!).  You could
also ground the metal in the chamber using metal wire touching something
metal outside the chamber (the Russian post-doc solution, very clever).
We keep 100% EtOH in a small container (about 100 ml) in the back of the
chamber to reduce static, which I learned from a long ago histonet post,
so I have no idea why it works.
Otherwise, buy an anti-static brush, which I hear exists, but have never
seen.

Emily
--
the velocity of time turns her voice into sugar water
http://www.youtube.com/watch?v=jNA6zzoObxg
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Re: [Histonet] cryostat blues

[Emily Sours]

Unfortunately, this sounds like static electricity.
Leica suggests spraying anti-static spray in the chamber (!).  You could
also ground the metal in the chamber using metal wire touching something
metal outside the chamber (the Russian post-doc solution, very clever).  We
keep 100% EtOH in a small container (about 100 ml) in the back of the
chamber to reduce static, which I learned from a long ago histonet post, so
I have no idea why it works.
Otherwise, buy an anti-static brush, which I hear exists, but have never
seen.

Emily
-- 
the velocity of time turns her voice into sugar water
http://www.youtube.com/watch?v=jNA6zzoObxg
___
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