RE: [Histonet] decalcification of articular cartilage

2011-04-21 Thread sgoebel
You could always get formical which fixes and decals at the same time.  This 
would eliminate the waiting for fixation.  Although I do usually let it fix in 
straight formalin overnight first.

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Thursday, April 21, 2011 12:59 PM
To: Nejat Yilmaz; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] decalcification of articular cartilage

We do a lot of IHC staining on bone and cartilage samples and we use 10% formic 
acid for decalcification for immunohistochemistry.  I'm afraid you may not be 
able to turnaround this in a week.  The tissue needs to be adequately fixed 
prior to decalcification, and properly decaled.  I would trim the tissue to 
about 4 mm thick if you can on a saw you might be able to turn this around in  
your time frame, but I have learned from experience if you rush decalcification 
you have a greater chance of running into problems.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz
Sent: Wednesday, April 20, 2011 8:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decalcification of articular cartilage

Dear Members,

We're planning to study sheep knee articular cartilage with histochemistry and 
immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde 
at the moment. We need an effective, quick and cartilage tissue protective 
decalcification method. We'll perform only light microscopic examination in 
this study and we have to complete the study within one week. In these 
circumstances what are your advices?
Thanks in advance.

Dr. Necat Yilmaz
University of Mersin
School of Medicine


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RE: [Histonet] decalcification of articular cartilage

2011-04-21 Thread Liz Chlipala
We do a lot of IHC staining on bone and cartilage samples and we use 10% formic 
acid for decalcification for immunohistochemistry.  I'm afraid you may not be 
able to turnaround this in a week.  The tissue needs to be adequately fixed 
prior to decalcification, and properly decaled.  I would trim the tissue to 
about 4 mm thick if you can on a saw you might be able to turn this around in  
your time frame, but I have learned from experience if you rush decalcification 
you have a greater chance of running into problems.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz
Sent: Wednesday, April 20, 2011 8:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decalcification of articular cartilage

Dear Members,

We're planning to study sheep knee articular cartilage with histochemistry and 
immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde 
at the moment. We need an effective, quick and cartilage tissue protective 
decalcification method. We'll perform only light microscopic examination in 
this study and we have to complete the study within one week. In these 
circumstances what are your advices?
Thanks in advance.

Dr. Necat Yilmaz
University of Mersin
School of Medicine


__ ESET NOD32 Antivirus Akıllı Güvenlik tarafından sağlanan bilgiler, 
virüs imza veritabanı sürümü: 6057 (20110420) __

İleti ESET NOD32 Antivirus Akıllı Güvenlik tarafından denetlendi.

http://www.nod32.com.tr

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