Re: [Histonet] Clearance Angle on Microtome Blade

[Barry Rittman via Histonet]

Rene thank you.
There is an excellent book on the section cutting that will give you any
theory you need.
Section Cutting in Microscopy. by H.F. Steedman. 1960. Blackwell Scientific
Publications. Oxford.
Also an American Optical Booklet on the Effective Use and Proper Care of
the Microtome.
Barry

On Sat, Jan 30, 2016 at 7:37 AM, Rene J Buesa via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> The type of tissue, the speed of sectioning, the knife bevel and the type
> of paraffin (melting point) influence the clearance angle.Anywhere from 5
> to 10º (preferable 5-6º) are the most used.René
>
> On Friday, January 29, 2016 3:17 PM, Kelli Goodkowsky via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
>
>  Good afternoon,
> Can someone touch base with me regarding the clearance angle of the
> microtome blade?  How would you describe a blade clearance angle that is
> too great or too slight, and what microtomy problems are you likely to see
> with each?  There seems to be conflicting information out there (or at
> least varying perspectives).
>
> Thank you!
> Kelli
>
> Kelli Goodkowsky, M.Ed., HT (ASCP)
> Director Clinical Education
> Histologic Science
> Goodwin College
> (860) 727-6917
> kgoodkow...@goodwin.edu
> http://www.goodwin.edu/
>
>
>
>
>
>
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Re: [Histonet] Clearance Angle on Microtome Blade

[Rene J Buesa via Histonet]

The type of tissue, the speed of sectioning, the knife bevel and the type of 
paraffin (melting point) influence the clearance angle.Anywhere from 5 to 10º 
(preferable 5-6º) are the most used.René 

On Friday, January 29, 2016 3:17 PM, Kelli Goodkowsky via Histonet 
 wrote:
 

 Good afternoon,
Can someone touch base with me regarding the clearance angle of the microtome 
blade?  How would you describe a blade clearance angle that is too great or too 
slight, and what microtomy problems are you likely to see with each?  There 
seems to be conflicting information out there (or at least varying 
perspectives).

Thank you!
Kelli

Kelli Goodkowsky, M.Ed., HT (ASCP)
Director Clinical Education
Histologic Science
Goodwin College
(860) 727-6917
kgoodkow...@goodwin.edu
http://www.goodwin.edu/






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