[Histonet] Histology Technical Specialist - Toronto
Good morning, I hope everyone is doing great! We wanted to reach out regarding a new Histology Field Technical Specialist with a world leader in cancer diagnostics. The position will ideally be based in Toronto, and will provide histology technical expertise to internal and external clients in Canada. The ideal candidate will have a background in histology and will be open to travel. Please e-mail directly at m...@personifysearch.com mailto:m...@personifysearch.com to learn more. Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 http://www.personifysearch.com/ www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: QIHC
Yes, it's part of the requirements to be able to take the exam. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, October 29, 2014 12:19 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] FW: QIHC From: Amy Johnson Sent: Wednesday, October 29, 2014 6:57 AM To: 'histonet-boun...@lists.utsouthwestern.edu' Subject: QIHC Do you need to have experience with ISH to take the QIHC test? Thanks, Amylin Johnson, BS HTL(ASCP) Associates in Pathology Wausau, WI 715-847-2130 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: slides for IHC's
We have a Bond III, and although we use the Apex slides from Leica, we are not required to do so. We have 3 different manufacturer's slides validated should the Apex slides go on backorder. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Thursday, October 30, 2014 12:38 PM To: 'Goins, Tresa'; Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's What type of instrumentation does everyone use? We use Ventana Benchmark Ultra and they want us to use Superfrost Plus slides only...curious if other have the same restrictions -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, October 29, 2014 1:53 PM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Mercedes Medical Starfrost adhesive for IHC and HE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides for IHC's I would like to know what kind of slides everyone else is using for their IHC's. I am having some problems with tissue falling off the slides during Target Retrieval. This happens mostly on fattier tissue. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: slides for IHC's
We are using our Leica Apex slides on the Ventana Ultra. No problems. Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mro...@sfmc.net http://www.sfmc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Thursday, October 30, 2014 11:38 AM To: 'Goins, Tresa'; Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's What type of instrumentation does everyone use? We use Ventana Benchmark Ultra and they want us to use Superfrost Plus slides only...curious if other have the same restrictions -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, October 29, 2014 1:53 PM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Mercedes Medical Starfrost adhesive for IHC and HE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides for IHC's I would like to know what kind of slides everyone else is using for their IHC's. I am having some problems with tissue falling off the slides during Target Retrieval. This happens mostly on fattier tissue. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor Location in the Lab
Hi Everyone: Even though many tissue processors are closed systems with fume filters, do people try to put them next to fume hoods or under some ventilation duct to add an extra layer of protection from solvent fumes? Thanks, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Tissue Processor Location in the Lab
Tim, We have a separate room for the tissue processors. We have a chemical fume hood for pouring all chemicals into carboys via a funnel that is in a hole cut in the hood work surface, and a carboy directly beneath. We also have a fume extraction system above each tissue processor that is designed to pull any fumes up and away from anyone working at that processor. It does not enclose the processor, but sits above it. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wheelock, Timothy R. Sent: Thursday, October 30, 2014 10:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Processor Location in the Lab Hi Everyone: Even though many tissue processors are closed systems with fume filters, do people try to put them next to fume hoods or under some ventilation duct to add an extra layer of protection from solvent fumes? Thanks, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fibronectin
Does anyone know of a reference lab doing this antibody on human FFPE slides? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: slides for IHC's
I found that my Leica Apex slides is not suitable for IHC. All the breast FFPE sections fall off after demasking into both FLEX buffers (high and low pH). I uses only Menzel Polysine slides with great success many years for any tissues for IHC. For HE and HC we uses ususal slides with gelatine adhesive. Histonet people's teached me for fatty tissues to use two main rules: 1. Thicknes slices for processing no more than 2 mm 2. Fixation in 10%NBF at least 24-48 hours. Maxim Peshkov, Russia, Taganrog. mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Solvent Recyclers
GRAC http://www.newcomersupply.com/product/gravity-recycling-alcohol-cartridge-tissue-processor-grac-tp I've used this system. It takes quite a while to run. It works. But, there is one big problem. If there is a possibility of Xylene or Propar contamination, it will quickly ruin the filter and cause problems with any system using the recycled ethanol. It doesn't take much to completely mess up the filter. But, it does have the possibility to save a lab quite a bit of money. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maryann Deathridge Sent: Thursday, October 30, 2014 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Solvent Recyclers Any recommendations for solvent recyclers? Due to space issues benchtop unit preferred. Dependability more preferred. Pros and cons? You may email if preferred. Thanks in advance for responses Maryann . madeathri...@pastnashville.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Solvent Recyclers
CBG http://www.cbgtechnologies.com/ Completely dependable. I've had mine for years with no problems at all. It runs forever! Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maryann Deathridge Sent: Thursday, October 30, 2014 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Solvent Recyclers Any recommendations for solvent recyclers? Due to space issues benchtop unit preferred. Dependability more preferred. Pros and cons? You may email if preferred. Thanks in advance for responses Maryann . madeathri...@pastnashville.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Solvent Recyclers
Ditto Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, October 30, 2014 3:45 PM To: madeathri...@pastnashville.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Solvent Recyclers CBG http://www.cbgtechnologies.com/ Completely dependable. I've had mine for years with no problems at all. It runs forever! Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maryann Deathridge Sent: Thursday, October 30, 2014 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Solvent Recyclers Any recommendations for solvent recyclers? Due to space issues benchtop unit preferred. Dependability more preferred. Pros and cons? You may email if preferred. Thanks in advance for responses Maryann . madeathri...@pastnashville.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help! Need someone to repair an IEC CTD Cryostat
Hi, I'm having extreme difficulty finding someone who can work on the old IEC CTDs in Southern California. The issue isn't with the microtome, it's with the compressor/refrigeration. I've had a few people tell me they deal with these machines, only to find out after many hours of labor that they don't have a clue. Does anyone have any contacts? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraformaldehyde fixation questions
After having a lot of problems using 100% Acetone as a fixative, I am going to try 4% paraformaldehyde in DPBS pH 7.4 for fixation of slides cut from fresh tissues snap frozen in OCT. My plan is to cut the slides at 5 or 6 uM and dry them for 2 hours in the hood, then fix them in the 4% p-f for 10 minutes. Then immediately immerse them in Room temp DPBS. Is 10 minutes fixation long enough? Would 30 minutes work better? Is there any advantage, or disadvantage of using 4C versus Room temp for Paraformaldehyde? I am concerned about the drying of the tissues, and tissue loss from the slide. I'm not sure which will be best, drying after cutting or drying after fixing. Years ago, I vaguely remember being told that you should never dry the tissue after fixing it and then re-wet it. So I am leaning towards drying after cutting, but keeping the slides wet after fixing. The goal is for me to get good fixation, not destroy epitopes, and avoid the tissue damage that I get when I fix with Acetone. Thanks Patrick. PS: My brief internet search stated that Fixation occurs faster at higher temperatures (which I knew) , and that Some antibodies are capricious so for them 4C is better. (= IHC myth that corresponds to find what works and then don't change it) Is it right to assume that 4C is going to be gentler on the tissues than Room temp. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Solvent Recyclers
ditto -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, October 30, 2014 3:45 PM To: madeathri...@pastnashville.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Solvent Recyclers CBG http://www.cbgtechnologies.com/ Completely dependable. I've had mine for years with no problems at all. It runs forever! Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maryann Deathridge Sent: Thursday, October 30, 2014 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Solvent Recyclers Any recommendations for solvent recyclers? Due to space issues benchtop unit preferred. Dependability more preferred. Pros and cons? You may email if preferred. Thanks in advance for responses Maryann . madeathri...@pastnashville.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antibodies for Microsatellite instability
hi all, our laboratory in our country,Uruguay, is asked to perform Microsatellite instability on colon and endometrial carcinomas. I need your input on what vendors and clones would be the most reliable for these IHQ tests. Thanks in advance to all who could enlighten me on this point. Carlos defeo histotechnologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
