Hello to all list members.
We participated on early trial on Mac - Mark with the same exact result 
described by Don C. Elfving. I will not add more to this description.
However
- the extent of union disorder was dependant on the variety, some much more 
susceptible compare to others.
- we are involved in cleaning up trees from viruses, viroïds and phytoplasms. 
The process includes a check for the trueness of the variety after cleaning. In 
fact even if we don't use meristem propagation in the process, we recover the 
new tree from a micro-grafted single bud. In that case you always take the risk 
of a mutation. That's why we always check the new cultivar. For stocks it can 
take a lot of time to check (making new layers...), not very compatible with 
modern economy timings...
- in our case if I remember well, the expectation for the MARK stock was so 
great, that propagation was driven through in vitro techniques. They are known 
for an effect of 'juvenelisation' (don't know the word in English) expected in 
younger tissues. This can explain in limited case à physiologic behavior of 
plants slightly modified.
- we cleaned, in 40 years, thousand of cultivars (stocks, pip fruit varieties, 
stone fruit, roses) in our labs. Negative effects can be counted on one hand 
fingers, but they are still possible and need to be monitored.

Regards

Jean Marc Jourdain
Ctifl
France
Jourdain at ctifl dot fr



________________________________
De : apple-crop@virtualorchard.net [mailto:[EMAIL PROTECTED] De la part de 
Con.Traas
Envoyé : jeudi 20 novembre 2008 10:49
À : Apple-Crop
Objet : RE: Apple-Crop: MARK vs Mac-9 root stock

Hello Don and Evan,
I have been interested in MAC 9 since I saw it growing in an Irish Bramley's 
Seedling (cooking apple) orchard. The grower in question was always very 
enthusiastic about its productivity, and indeed, he still has the trees today, 
and has not experienced breakages, though soil-line swelling is a 
characteristic. I think the important question is what happened to MAC9 when it 
was made virus free (accompanied by a name-change to Mark), to make it more 
prone to breakages?
For people like Evan, who are still enthusiastic about the original stock, it 
would be possible to take some original MAC 9 tissue, and make another attempt 
at eliminating viruses. However, if the result is going to be the same (more 
brittle unions), there would not be any point in doing this.
Con Traas
The Apple Farm
Cahir
Ireland

PS I wonder if these changes could be epigenetic. It has recently been 
established that it is not only the DNA code that we inherit from our parents 
that determines our makeup, but that events in their lives that affect their 
cells (but not DNA) can also be passed on in reproduction via some type of 
"cell memory".
The more we find out, the more we realise what we don't know.

________________________________
From: apple-crop@virtualorchard.net [mailto:[EMAIL PROTECTED] On Behalf Of 
Elfving, Donald C
Sent: 19 November 2008 22:54
To: Apple-Crop
Subject: RE: Apple-Crop: MARK vs Mac-9 root stock

Back in 1980 I was involved in planting apple trees from the 1980 NC-140 Apple 
Rootstock trial at the Hort. Research Institute of Ontario research station at 
Simcoe, Ontario, Canada.  Among the 9 rootstocks in that trial was what we were 
told was the original MAC-9.  The top was 'Starkspur Supreme Delicious'  For 
the first few years, the MAC-9 trees grew well and displayed remarkable 
precocity.  By about 5 years of age, however, their vigor began to diminish and 
they became quite spur-bound.  Examination of the trees showed clearly that all 
5 were suffering from what has become known as "soil-line swelling", a 
proliferation of tissue at the crown of the tree that significantly disrupts 
the vascular connection between root and scion.  I also was initially impressed 
with the early performance of these trees.  As a result, starting in 1986 I 
planted a lot of trees on what by then was being called 'Mark'.  Again, the 
initial performance of the 'Mark' trees was good, but within 3 years we were 
starting to see some evidence of breakage at the union and, within a few more 
years, clear evidence of "soil-line swelling".  The MAC-9 trees in the 1980 
NC-140 trial were propagated at least 7-8 years before the official release of 
'Mark', and we were told that they in fact were the original MAC-9.  If that is 
the case, then our experience indicates that both MAC-9 and 'Mark' displayed 
the same problem of uncontrolled tissue proliferation on the rootstock shank.

Regards to all,

Don C. Elfving, Horticulturist and Professor
Washington State University
Tree Fruit Research and Extension Center
1100 N. Western Ave.
Wenatchee, WA 98801-1230
(509) 663-8181, ext. 252
(509) 662-8714 (fax)
[EMAIL PROTECTED]
________________________________
From: apple-crop@virtualorchard.net [mailto:[EMAIL PROTECTED] On Behalf Of Evan 
B. Milburn
Sent: Wednesday, November 19, 2008 1:16 PM
To: Apple-Crop
Subject: Apple-Crop: MARK vs Mac-9 root stock

Hello all,
  Back in the late 70's and early 80's I was lucky to have known and closely 
followed the research of Dr. Robert Carlson, MI on his development of the MAC-9 
apple rootstock. Those of you that belonged and was active in the then, IDFTA 
as I was (and still am) kept  watching its testing and development. By the late 
80's I was con vined this rootstock was a real winner . Smaller than the M-26, 
More productive than M-9.
 For 1987 I had ordered some 15,000 trees  to plant that year but only could 
get about 500. These were planted and still very productive to this day. These 
will be removed next year only because the variety Red Del  is no longer 
viable.Than it was discovered the MAC-9 had a "virus" and had to be go thru 
radiation to clean it up. This was done and reintroduced as MARK.
   I planted the rest of my order in 1989.on this MARK. They were a disaster! 
By year five and six they were all removed because of the bud union problem I 
called cancer. Those of you who have planted them know what I mean.
  On the IDFTA trip to Australia 2000 we visited one grower who had a large 
block of Reds planted side by side. One block on MARK ,the other on MAC-9. Same 
results. MAC-9 were absolutely beautiful, the MARKS were a disaster.
  Question-- Has any one had the same experience? I realize not many out there 
have the true MAC-9.    To the 'experts out there;  What in the process of 
"cleaning up the virus" would make this kind of change ? Please do not tell me 
,as many have ,there was no change. I've seen my self and dearly paid for it!
  If any one wants to see the true MAC-9 come see them before the Fall of 2009.



           Evan B. Milburn,Grower
           Elkton Maryland
           www.milburnorchards.com<http://www.milburnorchards.com>



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