Re: [aroma.affymetrix] Re: Failed to identify CDF unit with unit name
Hello Henrik, here is my script basedir - Projects/DrugRep/GEOData/ srcdir = paste(basedir,AZD2,sep=) workdir = paste(basedir,Workdir,sep=) setwd(workdir) tarFile-paste(basedir,AZD2/GSE4757_RAW.tar,sep=) covdesc - paste(basedir,AZD2/GSE4757.covdesc,sep=) convertCdf(annotationData/chipTypes/HG-U133_Plus_2/HG-U133_Plus_2.cdf, annotationData/chipTypes/HG-U133_Plus_2/HG-U133_Plus_2,bin.cdf) gsedir- strsplit(basename(tarFile),'_')[[1]][1] print(paste(Untarring,tarFile)) write(paste(Untarring,tarFile), file=test.log, append = TRUE) # create a rawdata folder # create annotationData Folder dir.create(rawData,recursive=TRUE) #dir.create(annotationData,recursive=TRUE) # get annotation chip file if it does not exist in local folder chipType - HG-U133_Plus_2 datafolder - paste(rawData/,gsedir,/,chipType,sep=) dir.create(datafolder , recursive=TRUE) untar(tarFile,exdir=datafolder) # Gunzip all the cel files setwd(datafolder) getwd() celfiles - list.files(pattern=CEL.gz,ignore.case = TRUE) lapply(celfiles,gunzip) # Create Cdf object setwd(../../..) cdf - AffymetrixCdfFile$byChipType(chipType); cs - AffymetrixCelSet$byName(gsedir, cdf=cdf); # gcRMA background correction bc - GcRmaBackgroundCorrection(cs); #this is where it errors ## csB - process(bc); # RMA quantile normalization qn - QuantileNormalization(csB, typesToUpdate=pm); csN - process(qn); # RMA probe summarization plm - RmaPlm(csN, flavor=oligo); fit(plm); # Extract chip effects on the log2 scale ces - getChipEffectSet(plm); theta - extractMatrix(ces); rownames(theta) - getUnitNames(cdf); theta - log2(theta); and here is my session info sessionInfo(); R version 2.15.0 (2012-03-30) Platform: i386-pc-mingw32/i386 (32-bit) locale: [1] LC_COLLATE=English_United States.1252 [2] LC_CTYPE=English_United States.1252 [3] LC_MONETARY=English_United States.1252 [4] LC_NUMERIC=C [5] LC_TIME=English_United States.1252 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] aroma.affymetrix_2.6.0 affxparser_1.30.0 aroma.apd_0.2.3 [4] R.huge_0.4.1 aroma.light_1.28.0 aroma.core_2.6.0 [7] matrixStats_0.5.3 R.rsp_0.8.2R.cache_0.6.3 [10] R.devices_2.1.1R.filesets_1.1.5 digest_0.5.2 [13] R.utils_1.18.0 R.oo_1.10.1R.methodsS3_1.4.2 [16] e1071_1.6-1class_7.3-5 thanks, rajesh On Monday, November 19, 2012 11:04:15 AM UTC+5:30, Henrik Bengtsson wrote: Hi, I've never seen that before, but remove any *.tmp or *.tmp.tmp etc files and try again. If that does not work, then please 1. show your script exactly at it is, 2. show the output you get from library(aroma.affymetrix); sessionInfo(); /Henrik. On Sun, Nov 18, 2012 at 8:02 PM, Raj Patrao rlpa...@gmail.comjavascript: wrote: Thanks Much Henrik. I used the ACS file, but I am not able to go further than the process(). I am getting an error Error: Failed to rename temporary file: probeData/GSE4757,OBC/HG-U133_Plus_2/GSM107523.CEL.tmp.tmp - probeData/GSE4757,OBC/HG-U133_Plus_2/GSM107523.CEL.tmp In addition: Warning messages: 1: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/filef9050a139e3', reason 'Permission denied' 2: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef90ba31c36', reason 'Permission denied' 3: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef909434baf', reason 'Permission denied' 4: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef90690e1d4d', reason 'Permission denied' Thanks, Rajesh. On Saturday, November 3, 2012 3:24:39 PM UTC+5:30, Raj Patrao wrote: Hello All, I am getting the following error when doing process in aroma: csB - process(bc,verbose=TRUE); Background correcting data set... Computing probe affinities (independent of data)... [2012-11-03 15:10:02] Exception: Failed to identify CDF unit with unit name '1': annotationData/chipTypes/HG-U133_Plus_2/u133plus2gb_probe_tab at #09. getProbeSequenceData.AffymetrixCdfFile(this, safe = safe, verbose = verbose) - getProbeSequenceData.AffymetrixCdfFile() is in environment 'aroma.affymetrix' at #08. getProbeSequenceData(this, safe = safe, verbose = verbose) - getProbeSequenceData() is in environment 'aroma.affymetrix' at #07. computeAffinities.AffymetrixCdfFile(cdf, paths = probePath, ..., verbose = less(verbose)) - computeAffinities.AffymetrixCdfFile() is in environment 'aroma.affymetrix' at #06. computeAffinities(cdf, paths = probePath, ..., verbose =
Re: [aroma.affymetrix] Re: Failed to identify CDF unit with unit name
Did you retry by first remove any of those *.tmp or *.tmp.tmp etc and rerunning dataSet - GSE4757,OBC; chipType - HG-U133_Plus_2; cdf - AffymetrixCdfFile$byChipType(chipType); cs - AffymetrixCelSet$byName(dataSet, cdf=cdf); print(cs); bc - GcRmaBackgroundCorrection(cs); print(bc); csB - process(bc); print(csB); ? /Henrik On Mon, Nov 19, 2012 at 1:27 AM, Raj Patrao rlpat...@gmail.com wrote: Hello Henrik, here is my script basedir - Projects/DrugRep/GEOData/ srcdir = paste(basedir,AZD2,sep=) workdir = paste(basedir,Workdir,sep=) setwd(workdir) tarFile-paste(basedir,AZD2/GSE4757_RAW.tar,sep=) covdesc - paste(basedir,AZD2/GSE4757.covdesc,sep=) convertCdf(annotationData/chipTypes/HG-U133_Plus_2/HG-U133_Plus_2.cdf, annotationData/chipTypes/HG-U133_Plus_2/HG-U133_Plus_2,bin.cdf) gsedir- strsplit(basename(tarFile),'_')[[1]][1] print(paste(Untarring,tarFile)) write(paste(Untarring,tarFile), file=test.log, append = TRUE) # create a rawdata folder # create annotationData Folder dir.create(rawData,recursive=TRUE) #dir.create(annotationData,recursive=TRUE) # get annotation chip file if it does not exist in local folder chipType - HG-U133_Plus_2 datafolder - paste(rawData/,gsedir,/,chipType,sep=) dir.create(datafolder , recursive=TRUE) untar(tarFile,exdir=datafolder) # Gunzip all the cel files setwd(datafolder) getwd() celfiles - list.files(pattern=CEL.gz,ignore.case = TRUE) lapply(celfiles,gunzip) # Create Cdf object setwd(../../..) cdf - AffymetrixCdfFile$byChipType(chipType); cs - AffymetrixCelSet$byName(gsedir, cdf=cdf); # gcRMA background correction bc - GcRmaBackgroundCorrection(cs); #this is where it errors ## csB - process(bc); # RMA quantile normalization qn - QuantileNormalization(csB, typesToUpdate=pm); csN - process(qn); # RMA probe summarization plm - RmaPlm(csN, flavor=oligo); fit(plm); # Extract chip effects on the log2 scale ces - getChipEffectSet(plm); theta - extractMatrix(ces); rownames(theta) - getUnitNames(cdf); theta - log2(theta); and here is my session info sessionInfo(); R version 2.15.0 (2012-03-30) Platform: i386-pc-mingw32/i386 (32-bit) locale: [1] LC_COLLATE=English_United States.1252 [2] LC_CTYPE=English_United States.1252 [3] LC_MONETARY=English_United States.1252 [4] LC_NUMERIC=C [5] LC_TIME=English_United States.1252 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] aroma.affymetrix_2.6.0 affxparser_1.30.0 aroma.apd_0.2.3 [4] R.huge_0.4.1 aroma.light_1.28.0 aroma.core_2.6.0 [7] matrixStats_0.5.3 R.rsp_0.8.2R.cache_0.6.3 [10] R.devices_2.1.1R.filesets_1.1.5 digest_0.5.2 [13] R.utils_1.18.0 R.oo_1.10.1R.methodsS3_1.4.2 [16] e1071_1.6-1class_7.3-5 thanks, rajesh On Monday, November 19, 2012 11:04:15 AM UTC+5:30, Henrik Bengtsson wrote: Hi, I've never seen that before, but remove any *.tmp or *.tmp.tmp etc files and try again. If that does not work, then please 1. show your script exactly at it is, 2. show the output you get from library(aroma.affymetrix); sessionInfo(); /Henrik. On Sun, Nov 18, 2012 at 8:02 PM, Raj Patrao rlpa...@gmail.com wrote: Thanks Much Henrik. I used the ACS file, but I am not able to go further than the process(). I am getting an error Error: Failed to rename temporary file: probeData/GSE4757,OBC/HG-U133_Plus_2/GSM107523.CEL.tmp.tmp - probeData/GSE4757,OBC/HG-U133_Plus_2/GSM107523.CEL.tmp In addition: Warning messages: 1: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/filef9050a139e3', reason 'Permission denied' 2: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef90ba31c36', reason 'Permission denied' 3: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef909434baf', reason 'Permission denied' 4: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef90690e1d4d', reason 'Permission denied' Thanks, Rajesh. On Saturday, November 3, 2012 3:24:39 PM UTC+5:30, Raj Patrao wrote: Hello All, I am getting the following error when doing process in aroma: csB - process(bc,verbose=TRUE); Background correcting data set... Computing probe affinities (independent of data)... [2012-11-03 15:10:02] Exception: Failed to identify CDF unit with unit name '1': annotationData/chipTypes/HG-U133_Plus_2/u133plus2gb_probe_tab at #09. getProbeSequenceData.AffymetrixCdfFile(this, safe = safe, verbose = verbose) - getProbeSequenceData.AffymetrixCdfFile() is in environment 'aroma.affymetrix' at #08. getProbeSequenceData(this, safe = safe, verbose = verbose) - getProbeSequenceData() is in environment
[aroma.affymetrix] Re: Failed to identify CDF unit with unit name
Hentrik, I did delete all the files created and re-ran. I deleted complete folder structure below , as these files are created automatically . *probeData* * GSE4757,GRBC* * GSE4757,OBC* Also, I dont know on what basis these datasets are getting created (these are getting created in the process step), and if the OBC, GRBC annotations are same for all sets.As shown in my script :* * *for each of the series* *{ * * Untar it in a work dir* * Gunzip all celfiles* * set up cdf* * set up cel file set* * run background correction - here the probeData folder gets created* * * *} * I intend to process large number of series in a automated way, so I am trying these options. Thanks, Rajesh. On Saturday, November 3, 2012 3:24:39 PM UTC+5:30, Raj Patrao wrote: *Hello All,* * * *I am getting the following error when doing process in aroma: * csB - process(bc,verbose=TRUE); Background correcting data set... Computing probe affinities (independent of data)... [2012-11-03 15:10:02] Exception: Failed to identify CDF unit with unit name '1': annotationData/chipTypes/HG-U133_Plus_2/u133plus2gb_probe_tab at #09. getProbeSequenceData.AffymetrixCdfFile(this, safe = safe, verbose = verbose) - getProbeSequenceData.AffymetrixCdfFile() is in environment 'aroma.affymetrix' at #08. getProbeSequenceData(this, safe = safe, verbose = verbose) - getProbeSequenceData() is in environment 'aroma.affymetrix' at #07. computeAffinities.AffymetrixCdfFile(cdf, paths = probePath, ..., verbose = less(verbose)) - computeAffinities.AffymetrixCdfFile() is in environment 'aroma.affymetrix' at #06. computeAffinities(cdf, paths = probePath, ..., verbose = less(verbose)) - computeAffinities() is in environment 'aroma.affymetrix' at #05. bgAdjustGcrma.AffymetrixCelSet(structure(NA, .env = environment, class = c(AffymetrixCelSet, AffymetrixFileSet, AromaPlatformInterface, AromaMicroarrayDataSet, GenericDataFileSet, FullNameInterface, Object)), path = probeData/GSE4757,GRBC/HG-U133_Plus_2, verbose = structure(TRUE, .env = environment, class = c(Verbose, Object)), overwrite = FALSE, subsetToUpdate = NULL, typesToUpdate = pm, indicesNegativeControl = NULL, affinities = NULL, type = fullmodel, opticalAdjust = TRUE, gsbAdjust = TRUE, gsbParameters = NULL, .deprecated = FALSE) - bgAdjustGcrma.AffymetrixCelSet() is in environment 'aroma.affymetrix' at #04. bgAdjustGcrma(structure(NA, .env = environment, class = c(AffymetrixCelSet, AffymetrixFileSet, AromaPlatformInterface, AromaMicroarrayDataSet, GenericDataFileSet, FullNameInterface, Object)), path = probeData/GSE4757,GRBC/HG-U133_Plus_2, verbose = structure(TRUE, .env = environment, class = c(Verbose, Object)), overwrite = FALSE, subsetToUpdate = NULL, typesToUpdate = pm, indicesNegativeControl = NULL, affinities = NULL, type = fullmodel, opticalAdjust = TRUE, gsbAdjust = TRUE, gsbParameters = NULL, .deprecated = FALSE) - bgAdjustGcrma() is in environment 'aroma.affymetrix' at #03. do.call(bgAdjustGcrma, args = args) - do.call() is in environment 'base' at #02. process.GcRmaBackgroundCorrection(bc, verbose = TRUE) - process.GcRmaBackgroundCorrection() is in environment 'aroma.affymetrix' at #01. process(bc, verbose = TRUE) - process() is in environment 'aroma.core' Error: Computing probe affinities (independent of data)...done Background correcting data set...done *Here is my directory structure : * annotationData annotationData\chipTypes\HG-U133_Plus_2 11/03/2012 03:09 PMDIR . 11/03/2012 03:09 PMDIR .. 10/19/2012 10:49 AM99,044,850 HG-U133_Plus_2 - ascii.cdf 10/26/2012 09:49 PM26,251,783 HG-U133_Plus_2.cdf 10/29/2012 10:21 AM15,952,085 Hs133P_Hs_REFSEQ.cdf 07/14/2005 12:23 AM23,991,168 u133plus2gb_probe_tab rawData\GSE4757\HG-U133_Plus_2 10/26/2012 09:18 PMDIR . 10/26/2012 09:18 PMDIR .. 10/26/2012 09:17 PM13,555,389 GSM107522.cel 10/26/2012 09:17 PM13,556,785 GSM107523.cel 10/26/2012 09:17 PM13,560,384 GSM107524.cel 10/26/2012 09:17 PM13,556,716 GSM107525.cel 10/26/2012 09:17 PM13,552,046 GSM107526.cel 10/26/2012 09:17 PM13,552,689 GSM107527.cel 10/26/2012 09:17 PM13,553,222 GSM107528.cel 10/26/2012 09:17 PM13,552,445 GSM107529.cel 10/26/2012 09:17 PM13,551,059 GSM107530.cel 10/26/2012 09:17 PM13,550,482 GSM107531.cel 10/26/2012 09:17 PM13,550,367 GSM107532.cel 10/26/2012 09:17 PM13,550,546
[aroma.affymetrix] Re: Failed to identify CDF unit with unit name
Thanks Much Henrik. I used the ACS file, but I am not able to go further than the process(). I am getting an error Error: Failed to rename temporary file: probeData/GSE4757,OBC/HG-U133_Plus_2/GSM107523.CEL.tmp.tmp - probeData/GSE4757,OBC/HG-U133_Plus_2/GSM107523.CEL.tmp In addition: Warning messages: 1: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/filef9050a139e3', reason 'Permission denied' 2: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef90ba31c36', reason 'Permission denied' 3: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef909434baf', reason 'Permission denied' 4: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef90690e1d4d', reason 'Permission denied' Thanks, Rajesh. On Saturday, November 3, 2012 3:24:39 PM UTC+5:30, Raj Patrao wrote: *Hello All,* * * *I am getting the following error when doing process in aroma: * csB - process(bc,verbose=TRUE); Background correcting data set... Computing probe affinities (independent of data)... [2012-11-03 15:10:02] Exception: Failed to identify CDF unit with unit name '1': annotationData/chipTypes/HG-U133_Plus_2/u133plus2gb_probe_tab at #09. getProbeSequenceData.AffymetrixCdfFile(this, safe = safe, verbose = verbose) - getProbeSequenceData.AffymetrixCdfFile() is in environment 'aroma.affymetrix' at #08. getProbeSequenceData(this, safe = safe, verbose = verbose) - getProbeSequenceData() is in environment 'aroma.affymetrix' at #07. computeAffinities.AffymetrixCdfFile(cdf, paths = probePath, ..., verbose = less(verbose)) - computeAffinities.AffymetrixCdfFile() is in environment 'aroma.affymetrix' at #06. computeAffinities(cdf, paths = probePath, ..., verbose = less(verbose)) - computeAffinities() is in environment 'aroma.affymetrix' at #05. bgAdjustGcrma.AffymetrixCelSet(structure(NA, .env = environment, class = c(AffymetrixCelSet, AffymetrixFileSet, AromaPlatformInterface, AromaMicroarrayDataSet, GenericDataFileSet, FullNameInterface, Object)), path = probeData/GSE4757,GRBC/HG-U133_Plus_2, verbose = structure(TRUE, .env = environment, class = c(Verbose, Object)), overwrite = FALSE, subsetToUpdate = NULL, typesToUpdate = pm, indicesNegativeControl = NULL, affinities = NULL, type = fullmodel, opticalAdjust = TRUE, gsbAdjust = TRUE, gsbParameters = NULL, .deprecated = FALSE) - bgAdjustGcrma.AffymetrixCelSet() is in environment 'aroma.affymetrix' at #04. bgAdjustGcrma(structure(NA, .env = environment, class = c(AffymetrixCelSet, AffymetrixFileSet, AromaPlatformInterface, AromaMicroarrayDataSet, GenericDataFileSet, FullNameInterface, Object)), path = probeData/GSE4757,GRBC/HG-U133_Plus_2, verbose = structure(TRUE, .env = environment, class = c(Verbose, Object)), overwrite = FALSE, subsetToUpdate = NULL, typesToUpdate = pm, indicesNegativeControl = NULL, affinities = NULL, type = fullmodel, opticalAdjust = TRUE, gsbAdjust = TRUE, gsbParameters = NULL, .deprecated = FALSE) - bgAdjustGcrma() is in environment 'aroma.affymetrix' at #03. do.call(bgAdjustGcrma, args = args) - do.call() is in environment 'base' at #02. process.GcRmaBackgroundCorrection(bc, verbose = TRUE) - process.GcRmaBackgroundCorrection() is in environment 'aroma.affymetrix' at #01. process(bc, verbose = TRUE) - process() is in environment 'aroma.core' Error: Computing probe affinities (independent of data)...done Background correcting data set...done *Here is my directory structure : * annotationData annotationData\chipTypes\HG-U133_Plus_2 11/03/2012 03:09 PMDIR . 11/03/2012 03:09 PMDIR .. 10/19/2012 10:49 AM99,044,850 HG-U133_Plus_2 - ascii.cdf 10/26/2012 09:49 PM26,251,783 HG-U133_Plus_2.cdf 10/29/2012 10:21 AM15,952,085 Hs133P_Hs_REFSEQ.cdf 07/14/2005 12:23 AM23,991,168 u133plus2gb_probe_tab rawData\GSE4757\HG-U133_Plus_2 10/26/2012 09:18 PMDIR . 10/26/2012 09:18 PMDIR .. 10/26/2012 09:17 PM13,555,389 GSM107522.cel 10/26/2012 09:17 PM13,556,785 GSM107523.cel 10/26/2012 09:17 PM13,560,384 GSM107524.cel 10/26/2012 09:17 PM13,556,716 GSM107525.cel 10/26/2012 09:17 PM13,552,046 GSM107526.cel 10/26/2012 09:17 PM13,552,689 GSM107527.cel 10/26/2012 09:17 PM13,553,222 GSM107528.cel 10/26/2012 09:17 PM13,552,445 GSM107529.cel 10/26/2012 09:17 PM13,551,059 GSM107530.cel 10/26/2012 09:17 PM13,550,482
Re: [aroma.affymetrix] Re: Failed to identify CDF unit with unit name
Hi, I've never seen that before, but remove any *.tmp or *.tmp.tmp etc files and try again. If that does not work, then please 1. show your script exactly at it is, 2. show the output you get from library(aroma.affymetrix); sessionInfo(); /Henrik. On Sun, Nov 18, 2012 at 8:02 PM, Raj Patrao rlpat...@gmail.com wrote: Thanks Much Henrik. I used the ACS file, but I am not able to go further than the process(). I am getting an error Error: Failed to rename temporary file: probeData/GSE4757,OBC/HG-U133_Plus_2/GSM107523.CEL.tmp.tmp - probeData/GSE4757,OBC/HG-U133_Plus_2/GSM107523.CEL.tmp In addition: Warning messages: 1: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/filef9050a139e3', reason 'Permission denied' 2: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef90ba31c36', reason 'Permission denied' 3: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef909434baf', reason 'Permission denied' 4: In file.remove(pathnameT) : cannot remove file 'probeData/GSE4757,OBC/HG-U133_Plus_2/filef90690e1d4d', reason 'Permission denied' Thanks, Rajesh. On Saturday, November 3, 2012 3:24:39 PM UTC+5:30, Raj Patrao wrote: Hello All, I am getting the following error when doing process in aroma: csB - process(bc,verbose=TRUE); Background correcting data set... Computing probe affinities (independent of data)... [2012-11-03 15:10:02] Exception: Failed to identify CDF unit with unit name '1': annotationData/chipTypes/HG-U133_Plus_2/u133plus2gb_probe_tab at #09. getProbeSequenceData.AffymetrixCdfFile(this, safe = safe, verbose = verbose) - getProbeSequenceData.AffymetrixCdfFile() is in environment 'aroma.affymetrix' at #08. getProbeSequenceData(this, safe = safe, verbose = verbose) - getProbeSequenceData() is in environment 'aroma.affymetrix' at #07. computeAffinities.AffymetrixCdfFile(cdf, paths = probePath, ..., verbose = less(verbose)) - computeAffinities.AffymetrixCdfFile() is in environment 'aroma.affymetrix' at #06. computeAffinities(cdf, paths = probePath, ..., verbose = less(verbose)) - computeAffinities() is in environment 'aroma.affymetrix' at #05. bgAdjustGcrma.AffymetrixCelSet(structure(NA, .env = environment, class = c(AffymetrixCelSet, AffymetrixFileSet, AromaPlatformInterface, AromaMicroarrayDataSet, GenericDataFileSet, FullNameInterface, Object)), path = probeData/GSE4757,GRBC/HG-U133_Plus_2, verbose = structure(TRUE, .env = environment, class = c(Verbose, Object)), overwrite = FALSE, subsetToUpdate = NULL, typesToUpdate = pm, indicesNegativeControl = NULL, affinities = NULL, type = fullmodel, opticalAdjust = TRUE, gsbAdjust = TRUE, gsbParameters = NULL, .deprecated = FALSE) - bgAdjustGcrma.AffymetrixCelSet() is in environment 'aroma.affymetrix' at #04. bgAdjustGcrma(structure(NA, .env = environment, class = c(AffymetrixCelSet, AffymetrixFileSet, AromaPlatformInterface, AromaMicroarrayDataSet, GenericDataFileSet, FullNameInterface, Object)), path = probeData/GSE4757,GRBC/HG-U133_Plus_2, verbose = structure(TRUE, .env = environment, class = c(Verbose, Object)), overwrite = FALSE, subsetToUpdate = NULL, typesToUpdate = pm, indicesNegativeControl = NULL, affinities = NULL, type = fullmodel, opticalAdjust = TRUE, gsbAdjust = TRUE, gsbParameters = NULL, .deprecated = FALSE) - bgAdjustGcrma() is in environment 'aroma.affymetrix' at #03. do.call(bgAdjustGcrma, args = args) - do.call() is in environment 'base' at #02. process.GcRmaBackgroundCorrection(bc, verbose = TRUE) - process.GcRmaBackgroundCorrection() is in environment 'aroma.affymetrix' at #01. process(bc, verbose = TRUE) - process() is in environment 'aroma.core' Error: Computing probe affinities (independent of data)...done Background correcting data set...done Here is my directory structure : annotationData annotationData\chipTypes\HG-U133_Plus_2 11/03/2012 03:09 PMDIR . 11/03/2012 03:09 PMDIR .. 10/19/2012 10:49 AM99,044,850 HG-U133_Plus_2 - ascii.cdf 10/26/2012 09:49 PM26,251,783 HG-U133_Plus_2.cdf 10/29/2012 10:21 AM15,952,085 Hs133P_Hs_REFSEQ.cdf 07/14/2005 12:23 AM23,991,168 u133plus2gb_probe_tab rawData\GSE4757\HG-U133_Plus_2 10/26/2012 09:18 PMDIR . 10/26/2012 09:18 PMDIR .. 10/26/2012 09:17 PM13,555,389 GSM107522.cel 10/26/2012 09:17 PM13,556,785 GSM107523.cel 10/26/2012 09:17 PM13,560,384 GSM107524.cel 10/26/2012 09:17 PM13,556,716 GSM107525.cel 10/26/2012 09:17