Hi, I've been doing some test with two complete genomes using blastn and tblastx through the http://www.hpa-bioinfotools.org.uk/pise/double_act.htmlwebsite. I have installed ACT in my mac G4 1 Ghz 1,25 GRam, As I only have two options (blastn and tblasx) in this website, I have to get very different results using 0, 500 and 1000 values at the cutoff score. Pardon for my ignorance, but if I'm trying to identify genes in my query comparing with a subject, what score will be the best then for use?
thanks Andres Zuniga
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