Hi Astrid

That message means that one or more of the features in the tab file extends
beyond the end of the sequence. I would check the features in the file and
find which ones are not within the plasmid sequence. Do you have the
additional genes in there as well for example. If you let me know where the
files are I can have a look.

Regards
Tim


On 11/16/12 3:09 PM, "Astrid von Mentzer" <a...@sanger.ac.uk> wrote:

> I have run biggerblast and open all the files in ACT and it looks fine.
> So sequence one is fasta file which contains a plasmid and three additional
> genes (in that order). When I then try to load a .tab file with the
> annotations for the plasmid it says "one of the features in the entry has an
> out of range location 1..101857
> 
> So the .tab file only contains the annotation for the plasmid and not the
> three added genes... is that the problem?
> No way to come around this?
> 
> I want to look for several genes in a velvet assembled strain at the same time
> but need to load the annotation for the plasmid.
> 
> //Astrid
> ***************************
> Astrid von Mentzer, PhD-student
> University of Gothenburg
> Institute of Biomedicine
> Department of Microbiology and Immunology
> Box 435
> 405 30 Göteborg
> Sweden
> 
> Phone: +46 31 786 62 21
> Fax: +46 31 786 62 05
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