Hi Zeenia

This is a warning message. Artemis attempts to match the length of the
sequence in the GFF with the sequence lengths in the BAM header. If it
doesn't then it gives that message. It may be worth checking the header of
the BAM.

Regards
Tim


On 2/17/12 4:21 AM, "Zeenia Jagga" <zee...@icgeb.res.in> wrote:

> Dear Sir,
> I have generated a alignment file in .bam format(.bam format is sorted and
> indexed).
> I am facing problem while viewing it in artemis.
> 1. loaded reference file in .gff format.
> 2. From "Read BAM/VCF" tab, I load .bam file
> 
> error is generated "length of sequence loaded does not match the length of the
> default reference sequence in the BAM (psu|M76611)"
> 
> but  psu|M76611 which is mitochondrial genome of plasmodium is of same length
> in GFF file and the file from which index of .bam was generated.
> 
> Plz help to trouble-shoot this issue.
> 
> Regards,
> Zeenia Jagga
> 
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> Artemis-users@sanger.ac.uk
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