david vilanova wrote: > Hello, > I have tried with: > PixelSize="10" > ImageOrigin="4440,1000" > I have double checked the image within genepix and the coordinates > (expressed in µm) are correct (gpr file matches with the tiff file). 1µm > correspond to 10pixels in genepix.
I think it is the other way around. Each pixel is 10µm in size. See http://www.moleculardevices.com/pages/software/gn_genepix_file_formats.html#gpr X Offset: 4400 Y Offset: 1000 X and Y scale: 10 Spot size: try 32; this is given in pixels > However the spot image is not the expected one. Still getting black > squares and sometimes getting spots but a large set of spots instead of > only one. Is a zoom applied to the spot that should be seen , otherwise > i gets hards to view !?? As stated before the pixel coordinates are calculated as: pixelX = (rawX - offsetX) / scaleX and the same for the Y coordinates. > > It looks like soemthing is wrong and i don't know where to look ? Could > I get your gpr file and tiff image to try and see how it works ?? If you go to http://base.thep.lu.se/browser/trunk/src/test/data you will find everything you need. The file 'spots.tif' is the (two-channel) image and the 'test.rawdata.import.txt' is the corresponding raw data set. /Nicklas ------------------------------------------------------------------------- Take Surveys. Earn Cash. Influence the Future of IT Join SourceForge.net's Techsay panel and you'll get the chance to share your opinions on IT & business topics through brief surveys - and earn cash http://www.techsay.com/default.php?page=join.php&p=sourceforge&CID=DEVDEV _______________________________________________ The BASE general discussion mailing list basedb-users@lists.sourceforge.net unsubscribe: send a mail with subject "unsubscribe" to [EMAIL PROTECTED]
