[base] labeled extract amounts

2008-01-07 Thread Bob MacCallum

Hello BASEers

If I add two labeled extracts to a hyb (e.g. two colour array), there is only
one box for the used amount.  Does this mean that hyb'ing different amounts
for each channel is not possible (in BASE)?

many thanks,
Bob.

-- 
Bob MacCallum | VectorBase Developer | Kafatos/Christophides Groups |
Division of Cell and Molecular Biology | Imperial College London |
Phone +442075941945 | Email [EMAIL PROTECTED]

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[base] analysis table already exists

2008-01-07 Thread Bob MacCallum

Dear BASE team,

I just got the following error while creating a root bioassayset for a small
test experiment.  My hunch is that it's something bad like a corrupted db...

I plan to get 2.5 installed ASAP, but was hoping to do a demo with 2.4.5 to
my colleagues later this week. 

Well I am in luck, I just repeated exactly the same thing and it worked fine.

But the odd thing is that I now see that there are three identical-looking
bioassaysets in the experiment, even though I only ran the Formula intensity
calculator plugin twice.  From the Date column it is the latter analysis
which got duplicated (and the original one seems to work ok).  I am not
usually a double-clicker, but that might be one explanation.

Strange indeed.

Here's the stack trace in any case.

cheers,
Bob.

net.sf.basedb.core.BaseException: Table 'D13Pos' already exists
at net.sf.basedb.core.HibernateUtil.createVirtualTable(HibernateUtil.java:605)
at net.sf.basedb.core.VirtualDb.createTables(VirtualDb.java:290)
at 
net.sf.basedb.core.PositionBatcher.buildInsertSelectSql(PositionBatcher.java:303)
at net.sf.basedb.core.PositionBatcher.insert(PositionBatcher.java:246)
at 
net.sf.basedb.util.IntensityCalculatorUtil.createRootBioAssaySet(IntensityCalculatorUtil.java:204)
at 
net.sf.basedb.plugins.IntensityCalculatorPlugin.run(IntensityCalculatorPlugin.java:249)
at 
net.sf.basedb.core.PluginExecutionRequest.invoke(PluginExecutionRequest.java:89)
at net.sf.basedb.core.InternalJobQueue$JobRunner.run(InternalJobQueue.java:421)
at java.lang.Thread.run(Thread.java:619)
Caused by: com.mysql.jdbc.exceptions.MySQLSyntaxErrorException: Table 'D13Pos' 
already exists
at com.mysql.jdbc.SQLError.createSQLException(SQLError.java:936)
at com.mysql.jdbc.MysqlIO.checkErrorPacket(MysqlIO.java:2934)
at com.mysql.jdbc.MysqlIO.sendCommand(MysqlIO.java:1616)
at com.mysql.jdbc.MysqlIO.sqlQueryDirect(MysqlIO.java:1708)
at com.mysql.jdbc.Connection.execSQL(Connection.java:3249)
at com.mysql.jdbc.Statement.executeUpdate(Statement.java:1350)
at com.mysql.jdbc.Statement.executeUpdate(Statement.java:1266)
at 
com.mchange.v2.c3p0.impl.NewProxyStatement.executeUpdate(NewProxyStatement.java:64)
at net.sf.basedb.core.HibernateUtil.createVirtualTable(HibernateUtil.java:581)
... 8 more




Version BASE 2.4.6pre (build #3938; schema #40)
Web server  Apache Tomcat/5.5.20
Database Server MySQL 5.0.21-max-log
Database Dialectorg.hibernate.dialect.MySQLInnoDBDialect
JDBC Driver com.mysql.jdbc.Driver (version 5.0)
Java runtimeJava(TM) SE Runtime Environment (1.6.0-b105), Sun Microsystems 
Inc.
Operating systemLinux amd64 2.6.16.53-0.16-smp
Memory  Total: 474.6 MB
Free: 238.2 MB
Max: 910.3 MB

-- 
Bob MacCallum | VectorBase Developer | Kafatos/Christophides Groups |
Division of Cell and Molecular Biology | Imperial College London |
Phone +442075941945 | Email [EMAIL PROTECTED]

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Re: [base] labeled extract amounts

2008-01-07 Thread Nicklas Nordborg
Bob MacCallum wrote:
 Hello BASEers
 
 If I add two labeled extracts to a hyb (e.g. two colour array), there is only
 one box for the used amount.  Does this mean that hyb'ing different amounts
 for each channel is not possible (in BASE)?

No. The box applies to the item(s) that are currently selected in the 
list. If the current amounts are different, the last one is displayed. 
If you change the value, the new amount will be set on all selected items.

/Nicklas


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Re: [base] analysis table already exists

2008-01-07 Thread Nicklas Nordborg
I think the 'table exists' problem can happen in rare cases if two jobs 
are executed at the same time, the time window between checking if a 
table exists and creating it if it doesn't is small but not zero. This 
means that two different jobs may not see the table and both will try to 
create it... The last one will fail. I guess it is possible to make the 
code more robust in this case, but figured it would maybe not happen 
very often (if at all) since the tables are usually created at the same 
time as the first root bioassay set in an experiment is created. In any 
case, you don't need to worry about database being corrupted.

The duplication is a bit more surprising. Are the bioassay sets created 
by the same or different jobs? You can check the jobs table (View - 
Jobs) if you can't find it out from the experiment analysis page. The 
one thing that comes to my mind and that could explain both the 'table 
exists' error and the duplication issue is that each job is for some 
reason executed twice (and at the same time). It shouldn't happen, but I 
remember that we had some problems when running test programs and still 
having the web server's job queue active. Every now and then the web 
server would start executing a job that was also started by our test 
program. Some times the one executing on the web server would fail and 
some times the test program. So, do you have any job agents installed, 
or multiple web applications, or anything else that is executing jobs 
from the job queue?

/Nicklas


Bob MacCallum wrote:
 Dear BASE team,
 
 I just got the following error while creating a root bioassayset for a small
 test experiment.  My hunch is that it's something bad like a corrupted db...
 
 I plan to get 2.5 installed ASAP, but was hoping to do a demo with 2.4.5 to
 my colleagues later this week. 
 
 Well I am in luck, I just repeated exactly the same thing and it worked fine.
 
 But the odd thing is that I now see that there are three identical-looking
 bioassaysets in the experiment, even though I only ran the Formula intensity
 calculator plugin twice.  From the Date column it is the latter analysis
 which got duplicated (and the original one seems to work ok).  I am not
 usually a double-clicker, but that might be one explanation.
 
 Strange indeed.
 
 Here's the stack trace in any case.
 
 cheers,
 Bob.
 
 net.sf.basedb.core.BaseException: Table 'D13Pos' already exists
 at net.sf.basedb.core.HibernateUtil.createVirtualTable(HibernateUtil.java:605)
 at net.sf.basedb.core.VirtualDb.createTables(VirtualDb.java:290)
 at 
 net.sf.basedb.core.PositionBatcher.buildInsertSelectSql(PositionBatcher.java:303)
 at net.sf.basedb.core.PositionBatcher.insert(PositionBatcher.java:246)
 at 
 net.sf.basedb.util.IntensityCalculatorUtil.createRootBioAssaySet(IntensityCalculatorUtil.java:204)
 at 
 net.sf.basedb.plugins.IntensityCalculatorPlugin.run(IntensityCalculatorPlugin.java:249)
 at 
 net.sf.basedb.core.PluginExecutionRequest.invoke(PluginExecutionRequest.java:89)
 at 
 net.sf.basedb.core.InternalJobQueue$JobRunner.run(InternalJobQueue.java:421)
 at java.lang.Thread.run(Thread.java:619)
 Caused by: com.mysql.jdbc.exceptions.MySQLSyntaxErrorException: Table 
 'D13Pos' already exists
 at com.mysql.jdbc.SQLError.createSQLException(SQLError.java:936)
 at com.mysql.jdbc.MysqlIO.checkErrorPacket(MysqlIO.java:2934)
 at com.mysql.jdbc.MysqlIO.sendCommand(MysqlIO.java:1616)
 at com.mysql.jdbc.MysqlIO.sqlQueryDirect(MysqlIO.java:1708)
 at com.mysql.jdbc.Connection.execSQL(Connection.java:3249)
 at com.mysql.jdbc.Statement.executeUpdate(Statement.java:1350)
 at com.mysql.jdbc.Statement.executeUpdate(Statement.java:1266)
 at 
 com.mchange.v2.c3p0.impl.NewProxyStatement.executeUpdate(NewProxyStatement.java:64)
 at net.sf.basedb.core.HibernateUtil.createVirtualTable(HibernateUtil.java:581)
 ... 8 more
 
 
 
 
 Version   BASE 2.4.6pre (build #3938; schema #40)
 Web serverApache Tomcat/5.5.20
 Database Server   MySQL 5.0.21-max-log
 Database Dialect  org.hibernate.dialect.MySQLInnoDBDialect
 JDBC Driver   com.mysql.jdbc.Driver (version 5.0)
 Java runtime  Java(TM) SE Runtime Environment (1.6.0-b105), Sun Microsystems 
 Inc.
 Operating system  Linux amd64 2.6.16.53-0.16-smp
 MemoryTotal: 474.6 MB
 Free: 238.2 MB
 Max: 910.3 MB
 


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