Re: [Bioc-devel] Package size issue

2024-03-28 Thread Ali Sajid Imami
Hi, I am not part of the core Bioconductor team, but I have been in this situation before. I would suggest you use the BFG Repo Cleaner ( https://rtyley.github.io/bfg-repo-cleaner/) to remove the errant bam file from your repo's history. It's very likely that adding and removing the bam file means

[Bioc-devel] Package size issue

2024-03-28 Thread gabriel.villa...@mdc-berlin.de
Hi! This is my first message to the mailing list so apologies if I am breaking any guidelines. I am submitting a package called Ribostan and the build report gives an error that the package tarball exceeds the size requirement. I am also being warned that individual package files exceed the

Re: [Bioc-devel] Bioconductor 3.19 db0s, OrgDbs, and TxDbs now available

2024-03-28 Thread Robert Castelo
hi, related to this, I found recently the problem that the TxDb.Hsapiens.UCSC.hg38.knownGene package contains only a few of the RN7SL (RNA component of signal recognition particle 7SL) genes and I suspect the problem is that on the one hand UCSC currently provides the 'knownGene' track based

Re: [Bioc-devel] Bioconductor 3.19 db0s, OrgDbs, and TxDbs now available

2024-03-28 Thread James W. MacDonald
There are EnsDbs for Ensembl builds 87-111 that Johannes Rainier submits to the AnnotationHub for those who want to use Ensembl mappings. And a direct build of a TxDb using a UCSC GTF file has the chrM genes as well. > ensdb <- hub[["AH100643"]] > subsetByOverlaps(transcriptsBy(ensdb),

[Bioc-devel] Issue with lme4/Matrix in BioC 3.19 R 4.4

2024-03-28 Thread Hoffman, Gabriel via Bioc-devel
Due to a known issue (https://github.com/lme4/lme4/issues/775#issuecomment-2024392388) running lme4::lmer() fails in BioC 3.19 using R 4.4 2024-03-18 r86148 on the build site because of a discrepancy between the Matrix version and the version used when lme4 is compiled. The solution is to

Re: [Bioc-devel] Bioconductor 3.19 db0s, OrgDbs, and TxDbs now available

2024-03-28 Thread Tim Triche, Jr.
is this an argument in favor of using ENSEMBL gene and transcript IDs rather than ENTREZ or UCSC? Or just changing the way that the databases are keyed? There really ought not to be transcripts for a gene on a different chromosome from the gene, although the MHC and KIR loci (with alt contigs)

Re: [Bioc-devel] Bioconductor 3.19 db0s, OrgDbs, and TxDbs now available

2024-03-28 Thread Tim Triche, Jr.
yep, looks like that's the problem I wonder why it doesn't have a CDS chromosome in ENSEMBL? That's a bit nuts. It is indeed indexed to MT in the canonical ENSEMBL database: https://useast.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG0198727;r=MT:14747-15887;t=ENST0361789 --t On Thu,

Re: [Bioc-devel] Bioconductor 3.19 db0s, OrgDbs, and TxDbs now available

2024-03-28 Thread James W. MacDonald
As well as > subsetByOverlaps(transcripts(Homo.sapiens), GRanges("chrM:1-16569")) 'select()' returned 1:1 mapping between keys and columns GRanges object with 37 ranges and 2 metadata columns: seqnames ranges strand | TXIDTXNAME | [1]

Re: [Bioc-devel] Bioconductor 3.19 db0s, OrgDbs, and TxDbs now available

2024-03-28 Thread Vincent Carey
winging it here tim > select(Homo.sapiens, keys="ENSG0198727", keytype="ENSEMBL", columns=c("GENENAME", "GENEID", "CDSCHROM", "SYMBOL")) 'select()' returned 1:1 mapping between keys and columns ENSEMBL GENENAME SYMBOL CDSCHROM GENEID 1 ENSG0198727 cytochrome b CYTB

Re: [Bioc-devel] Bioconductor 3.19 db0s, OrgDbs, and TxDbs now available

2024-03-28 Thread Tim Triche, Jr.
Hi Lori and fellow maintainers, I had a strange experience yesterday where I pulled down genes and transcripts from Homo.sapiens, only to discover that all mitochondrial encoded genes (MT-CYB, MT-CO2, etc) were missing. Is there an historical reason why this is so? Obviously these transcripts

[Bioc-devel] Bioconductor 3.19 db0s, OrgDbs, and TxDbs now available

2024-03-28 Thread Kern, Lori via Bioc-devel
Hello Bioconductor community, The newest db0, OrgDb, and TxDb annotation packages for the upcoming Bioconductor 3.19 release are up and available for download in the devel version of Bioconductor. The deadline for submitting contributed annotation packages will be Wednesday April 17 th. The

Re: [Bioc-devel] CuvierLab -Request to add maintainers for HicAggR

2024-03-28 Thread Olivier Cuvier
Dear Lori Sheperd - Kern Awesome, thank you very much. Have a great day :) Oliver De: "Kern, Lori" À: "Olivier Cuvier" , "bioc-devel" Envoyé: Jeudi 28 Mars 2024 12:19:38 Objet: Re: CuvierLab -Request to add maintainers for HicAggR I can assist with this and will be in touch with

Re: [Bioc-devel] CuvierLab -Request to add maintainers for HicAggR

2024-03-28 Thread Kern, Lori via Bioc-devel
I can assist with this and will be in touch with Dr. Tesfaye to set up a BiocCredentials account. Lori Shepherd - Kern Bioconductor Core Team Roswell Park Comprehensive Cancer Center Department of Biostatistics & Bioinformatics Elm & Carlton Streets Buffalo, New York 14263

[Bioc-devel] CuvierLab -Request to add maintainers for HicAggR

2024-03-28 Thread Olivier Cuvier
Dear Colleagues I'd like to add Dr. Tesfaye - rob.t...@gmail.com - as a maintainer of CuvierLab HicAggR GitHub-Bioconductor package. Thank you for your help Best regards Oliver Olivier Oliver Cuvier - Group Leader (Director of Med Research (DR) / Inserm)