Re: [Bioc-devel] FlipFlop Ignores Read Strand and Requires Antiquated File Formats

2016-11-01 Thread Wolfgang Huber
Dear Dario These all look like legitimate suggestions, and probably the package author has something to say on their substance. But independent of the specifics here, I think we should also keep in mind that the motivations for package submission vary across the project. Some contributors

[Bioc-devel] FlipFlop Ignores Read Strand and Requires Antiquated File Formats

2016-11-01 Thread Dario Strbenac
Hello, The package FlipFlop is made for isoform quantitiation. Why are there no options to specify the RNA-seq read strand ? Otherwise, the method produces incorrect counts where overlapping genes on both strands are being transcribed. Also, the software requires a SAM file as input. This is