Re: [Bioc-devel] From Biostring matching to short read mapping

2019-11-09 Thread Bhagwat, Aditya
Thankyou Michael, I got Rbowtie working, now functionalizing it for use within multicrispr. I noticed that in QuasR, you actually create a package with bowtie indices which you then use for future purposes. Interesting workflow, think I will make use of that functionality. Thankyou Herve. Yes,

Re: [Bioc-devel] From Biostring matching to short read mapping

2019-11-09 Thread Éric Fournier
Hi, it might be worthwhile to note that the concern about different chromosome sizes only applies if you have more workers than chromosomes. If you're running on 2-8 threads, the longer chromosome might hold up a thread while another processes two short ones. Cheers, -Eric

Re: [Bioc-devel] From Biostring matching to short read mapping

2019-11-09 Thread Wei Shi
Hi Aditya, Yes you are correct that Subread reports no more than 16 alignments per reads. One reason for this limitation is because subread detects indels in the read (Bowtie does not detect indels) and it has to set a limit on the number of candidate locations being considered due to the

Re: [Bioc-devel] From Biostring matching to short read mapping

2019-11-09 Thread Pages, Herve
On 11/9/19 08:12, Éric Fournier wrote: > Hi, > > it might be worthwhile to note that the concern about different chromosome > sizes only applies if you have more workers than chromosomes. If you're > running on 2-8 threads, the longer chromosome might hold up a thread while > another processes