Thankyou Michael, I got Rbowtie working, now functionalizing it for use within
multicrispr. I noticed that in QuasR, you actually create a package with bowtie
indices which you then use for future purposes. Interesting workflow, think I
will make use of that functionality.
Thankyou Herve. Yes,
Hi,
it might be worthwhile to note that the concern about different chromosome
sizes only applies if you have more workers than chromosomes. If you're running
on 2-8 threads, the longer chromosome might hold up a thread while another
processes two short ones.
Cheers,
-Eric
Hi Aditya,
Yes you are correct that Subread reports no more than 16 alignments per reads.
One reason for this limitation is because subread detects indels in the read
(Bowtie does not detect indels) and it has to set a limit on the number of
candidate locations being considered due to the
On 11/9/19 08:12, Éric Fournier wrote:
> Hi,
>
> it might be worthwhile to note that the concern about different chromosome
> sizes only applies if you have more workers than chromosomes. If you're
> running on 2-8 threads, the longer chromosome might hold up a thread while
> another processes