RE: [Biojava-l] topic about RestrictionEnzyme

2005-05-12 Thread george waldon
Oups! My mistake (and apologies) about the constructors and cutType. Now, I must disagree with the overhangs. When the cut is not bluntended, it generates a little asymetrical overhang or piece of single stranded DNA which is designated by its location at the end 3' or 5' of the strand of DNA it

Re: [Biojava-l] topic about RestrictionEnzyme

2005-05-12 Thread zhenqing ye
>BaeI generates 3' overhangs on both sides so DownstreamEndType equals 1 >(OVERHANG_3PRIME). i don't think so. you can refer the record in rebase database. <1>BaeI <2> <3>(10/15)ACGTAYC(12/7) 5' 3' N N

RE: [Biojava-l] topic about RestrictionEnzyme

2005-05-11 Thread george waldon
>From zhenqing ye: 0(#DownstreamEndType) 24 (#DownstreamCut[0]) 19 (#DownstreamCut[1]) BaeI generates 3' overhangs on both sides so DownstreamEndType equals 1 (OVERHANG_3PRIME). Looking at the code, I just found another bug. In RestrictionEnzyme.java, the first public cons

Re: [Biojava-l] topic about RestrictionEnzyme

2005-05-10 Thread mark . schreiber
I was going to patch this in CVS but it seems Richard beat me to it! Mark Schreiber Principal Scientist (Bioinformatics) Novartis Institute for Tropical Diseases (NITD) 10 Biopolis Road #05-01 Chromos Singapore 138670 www.nitd.novartis.com phone +65 6722 2973 fax +65 6722 2910 zhenqing ye

Re: [Biojava-l] topic about RestrictionEnzyme

2005-05-10 Thread mark . schreiber
Hi - This does look like an error although I'm no expert on restriction enzymes. Can anyone else comment? If you do find the source of the error please let us know. - Mark zhenqing ye <[EMAIL PROTECTED]> Sent by: [EMAIL PROTECTED] 05/10/2005 03:04 PM To: biojava-l@biojava.or