[ccp4bb] Molecular replacement and refinement trouble

2007-02-05 Thread Jenny
Hi, Dear all, Firstly ,I'd like to thank all the people for their kind help, especially Miguel, Bernie ,Mark, Moody, Eleanor, Eaton , Peter and lots of others Without your help, I wouldn't make it this far. Secondly, I'd like to share some of the experiences. We had a hard time trying to do

Re: [ccp4bb] Removal of cold gas layer for cryocooling

2007-02-05 Thread James Holton
To add to your survey: Yes I have tried it. Yes it does work better for glassifying cryobuffers. I saw Rob Thorne give a talk on this some time ago and immediately went home to give it a try. I used a cryobuffer (25% (v/v) glycerol; 0.6N NaOH; 25 mM SeMet) I had been having hit-or-miss

[ccp4bb] DPC-peptide crystal

2007-02-05 Thread shivesh kumar
Dear all I am trying to crystallize one DPC bound small peptide.Small cystals are appearing.How can I grow it bigger for mounting.Any suggestions are welcome.Thanx in advance. Shivesh

Re: [ccp4bb] omit map on OMIT ccp4 program

2007-02-05 Thread Nikos Pinotsis
Dear Taiana, thy the updated info according to the new sfcheck style in: http://www.ysbl.york.ac.uk/~alexei/sfcheck.html or simply type sfcheck -h What worked for me recently (and also described in the link above) was simply typing sfcheck -f mydata.mtz -m mymodel.pdb -omit 2 -map you will