Re: [ccp4bb] Arp/warp space group P 21 2 21

yes, actually my experience with P 21 2 21 dates back to midyear 2007. Retesting with the current refmac version runs fine. Clemens Quoting Ian Tickle [EMAIL PROTECTED]: Victor 'P 21 2 21' *is* the conventional indexing if a = b = c, i.e. it's the setting agreed for deposition of crystal

Re: [ccp4bb] Arp/warp space group P 21 2 21

Thanks very much to everybody for useful discussion on space groups, axes and ARP/wARP. I would particularly refer to the argument on space group uncertainty in data processing and a need to have convenient means to screen various space groups and axes settings for, e.g. automated molecular

Re: [ccp4bb] CCP4 Coot on Ubuntu

Thanks to all of you who replied , after installing 32bit library files. everything appears to work great.. John On Tue, Jun 10, 2008 at 8:41 PM, Martyn Winn [EMAIL PROTECTED] wrote: Yes, I think you need to download some 32 bit system libraries. Search synaptic for lib32 package

Re: [ccp4bb] Article needed

I have received the article, thanks for all. On Wed, Jun 11, 2008 at 3:11 PM, bharath SR [EMAIL PROTECTED] wrote: attached ,... On Wed, Jun 11, 2008 at 4:48 PM, Jayashankar [EMAIL PROTECTED] wrote: *Dear Friends,* I am a research student , looking for an article mentioned below. I

Re: [ccp4bb] tri and tetra problem

Debajyoti Dutta wrote: Hi all, I have a dataset giving contradictory results. When I am trying the molecular replacement with Amore and Molrep autoMR it is automatically taking trimer but upon running BALBES it is taking tetramer. The cell content analysis is also supporting the trimer

[ccp4bb] topology diagram

Hi all, wondering if anyone knows of a user friendly and esy to use program for generating topology diagrams for proteins. Any help would be very useful to me. thanks, Neeraj -- Neeraj Kapoor TPCB Graduate Fellow Sakmar Lab/ Molecular Biology Biochemistry The Rockefeller University

[ccp4bb] Heavy Atom Derivative Site

Hi All, I am looking for a service tool on the web where I can submit my protein sequence and obtain data information of the Heavy Atom (concentration,time for soaking etc) that will be compatible with the sequence I provide. One of the known site to me http://hatodas.harima.riken.go.jp/ does

Re: [ccp4bb] Heavy Atom Derivative Site

Hi Nilofer, We have just published a paper in Acta Cryst D which gives some of this information. Agniswamy J, Joyce MG, Hammer CH, Sun PD. Towards a rational approach for heavy-atom derivative screening in protein crystallography. Acta Cryst. (2008). D64, 354-367

Re: [ccp4bb] topology diagram

Hi Neeraj, CCP4 has a program which allows one to draw topology diagram manually...it's easy to learn and use. http://stein.bioch.dundee.ac.uk/~charlie/software/topdraw/ There is another program called TOPS (website is currently under maintenance) which generates topology diagram according to

[ccp4bb] Activity of a mutant enzyme compared to wild type - puzzle

Dear all: I have a single residue mutant whose enzyme activity is about 50% of the wild type. Interestingly, the mutation is in a region that involves a secondary site but not the active site. The two structures with or without ligands fit well (0.18 A) and the metal binding and cofactor

[ccp4bb] birefringent spacegroups

Dear Crystallographers, is there a list somewhere of spacegroups which can and cannot be birefringent? Upon what feature of the spacegroup does this depend? Jacob Keller *** Jacob Pearson Keller Northwestern University Medical Scientist Training

Re: [ccp4bb] Activity of a mutant enzyme compared to wild type - puzzle

Narayanan Ramasubbu wrote: Dear all: I have a single residue mutant whose enzyme activity is about 50% of the wild type. Interestingly, the mutation is in a region that involves a secondary site but not the active site. The two structures with or without ligands fit well (0.18 A) and the

Re: [ccp4bb] birefringent spacegroups

All cubic crystals, as far as I am aware, will not birefringe. All others can (but it may be weak for reasons that don't depend on symmetry). On Wed, Jun 11, 2008 at 5:33 PM, Jacob Keller [EMAIL PROTECTED] wrote: Dear Crystallographers, is there a list somewhere of spacegroups which can and

Re: [ccp4bb] Activity of a mutant enzyme compared to wild type - puzzle

Hi, Others have suggested very rational possibilities, so I would like to mention the odd one out: It may sound strange but I have to ask - 50% activity as measured how? Many biological assays have intrinsic experimental errors around 10%. If you add on top of this the difficulties encountered