[ccp4bb] Vacancy posting: PhD student position in structural biology / protein crystallography

A PhD student position in protein crystallography / structural biology is available in the Laboratory for Biocrystallography, Department of Pharmaceutical Sciences, University of Leuven, Belgium (http://pharm.kuleuven.be/anafar/). The PhD student will participate in one or more high-impact

Re: [ccp4bb] alternatives to IZIT

Whatever dye(s) you use, be sure to run some positive and negative controls to see how the dye really works. Jim On Sat, 14 Jun 2008, Mark Del Campo wrote: Before I place an order for some Izit, are there some other dyes I can use to check if I've got a protein crystal? Thanks, Mark

[ccp4bb] To improve xtal quality

Dear all, I am trying to improve the quality of a xtal, crystallizing with MPD as a precipitant at 16 degree C with thin plate like xtals. I would appreciate suggestions to have diffraction quality xtals (thick). I am using proline, glycine, glucose, sucrose as an additive.Thanx in advance.

Re: [ccp4bb] To improve xtal quality

Shivesh If you have not already, maybe try the following: 1. Adding other alcohols as an additive. See work by Keichi Namba in improving his flagella crystals. The idea is to poison growth in one direction. I have also had success in thickening crystals grown in MPD, by addition of 1-3%

Re: [ccp4bb] alternatives to IZIT

Several people have asked about the concentrations we use for various dyes. In the case of fluorescein, we started with 45mg/ml as a stock solution. This is quite arbitrary and we often found that our initial stock solutions needed to be diluted 1:10 or 1:100 before use. It is more art

Re: [ccp4bb] To improve xtal quality

Hi, I had the same problem with my crystals because they grew as thin plates. My precipitant was PEG8000, and the crystals grew thicker with addition of glycerol or ethylene glycol. Best, Kianoush --- On Mon, 6/16/08, shivesh kumar lt;[EMAIL PROTECTED]gt; wrote: From: shivesh kumar lt;[EMAIL

[ccp4bb] How many reflections for Rfree?

Dear All, It has recently been put to me that 5-10% of reflections should always be set aside for calculation of Rfree. However, when one has, say, 200 000 reflections (high resolution and/or large asymmetric unit), that seems to me to be a waste, because it would mean removing 10 000 to

Re: [ccp4bb] How many reflections for Rfree?

Mark et al. Rfree can be calculated with as few as 150 reflections, depending on the desired precision of Rfree. The real issue is scaling. Many programs scale vs. the test set independently, which becomes unstable with a small test set, and this is the main cause of error in Rfree. The topic

[ccp4bb] Scientific Support Officer position open at the Australian Synchrotron.

Dear All The PX group has a position open for a Scientific Support Officer. We operate two PX beamlines at the Australian Synchrotron in Melbourne Australia. Information about the position can be found at: http://www.synchrotron.org.au/content.asp?document_id=5390 I look forward to seeing

[ccp4bb] Call for proposals: Australian Synchrotron.

Dear All, We are now accepting general user proposals for Run 3 2008 (September – December) for beamline 3-BM1 The deadline for submission of proposals is July 1st 2008 The details on how to submit are outlined below: Information of submission of proposals