Dear Alexandra,
I bought this one recently and it is very nice. Very low vibration and
enough space for about 25 24-well plates plus maybe 50 96-well plates.
The only problem I had is that in my case the temperature reading on the
external LCD display is slightly off compared to the 'real'
Hi Sam
As I said, if you have NCS (doesn't matter whether you restrained it or not)
you need to be aware that the results may not be accurate, because there are
well-documented ways in which NCS can affect Rfree completely unpredictably.
With that in mind, if you refer to our Acta D paper
Hi Artem Evdokimov
Thank you for the mail. I have synthesized DMT-on
oligos in our laboratory. Deprotection was performed
treating with ammonium hydroxide for 15 hours at 55C.
Then, DMT-on oligo was separated from off using
RPHPLC.
DMT was cleaved by treating with 20% glacial acetic
acid for one
Check the purity of the DNA in solution:
A(260 nm)/A(280) = 1.8 for fully deprotected DNA, and you should see a
nice clean simple curve with a peak very close to 260 nm.
Check it on a denaturing gel. Smearing indicates incomplete
deprotection. This is usually the cause of solubility
Hi
Thank you for the mail.
It seems your correct. A(260 nm)/A(280) of one oligo
is around 1.0 and peak is around 272. Other
oligo's(260 nm)/A(280) is around 1.5.
Can I know what is the absorbance peak of base
protecting N-benzoyl group.
Is it possible to do deprotection of base after mixing
I think that at this point you're better off looking at a professional
literature.
For example:
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=102833
Artem
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of E
rajakumar
Sent: Sunday, June 22,
