[ccp4bb] RP-LC validation paper

for our research project we are on the lookout of following full text paper if anyone can kindly assist me in this Validation of an RP-LC Method and Assessment of rhG-CSF in Pharmaceutical Formulations by Liquid Chromatography and Biological Assay Authors: Sergio Luiz Dalmora a; Silvia Maria

[ccp4bb] Using the BB for file sharing

Dear All, There seems to be an increasing number of requests for articles on the BB. Can we assume that those supplying articles hold the necessary copyright permissions? Since the BB is visible and archived, I just want to be sure everything is nice and legal. regards Martyn --

[ccp4bb] PEPCAT or similar software

Dear All Sorry for the off-topic question. I am trying to classify peptides by populations in a molecular dynamics simulation. I got the reference to the PEPCAT software that can do that, but the web site is down (http://www.ludwig.edu.au/pepcat/index.html). Do you know if this software is still

Re: [ccp4bb] PST in refinement

One question - do you have two pairs of molecules, each related by the PST or is there extra non-crystallographic translation ? Averaging or NCS restraints dont give much extra information for molecules in the same orientation. Certainly any pseudo translation will generate sets of weak and

Re: [ccp4bb] Using the BB for file sharing

May I also suggest that the more traditional avenue - requesting the reprint from the corresponding author - is always tried first? I used to do this back in time when I was a poor russian grad student with zero access to full-text articles. Also handy if you collect stamps :) On Tue,

Re: [ccp4bb] PST in refinement

I have four molecules arranged such that molecules a and b are related by PST and adopt the same orientation as do molecules c and d, but there is no pst between the two pairs. All molecules lie in the same plane. A B top view C

Re: [ccp4bb] Using the BB for file sharing

It is impossible to know when someone has an anonymous user name and email address. I would suggest, minimally, that if someone asks us to send a paper, they need to sign their correspondence unambiguously (name and institution). Also, several requests in the span of three weeks from the

[ccp4bb] Question re: ice rings in diffraction data

Hello all, I have a query re: processing data.  I have some lovely diffraction data that has been marred by ice rings (so...not as lovely as it could be).  I was told it may be possible to mask the regions of the ice rings (obviously sacrificing completeness) during processing.  I have been

Re: [ccp4bb] RP-LC validation paper

Also, I wonder if word has gotten out that we've become a literature search service rather than a crystallography bulletin board. A lot of these papers seemingly have little to do with what we do... On Jul 15, 2008, at 1:24 AM, Meg wrote: for our research project we are on the lookout of

Re: [ccp4bb] Question re: ice rings in diffraction data

One can try to set the high resolution to exlude the ice ring ONLY during autoindexing. Once Denzo picks the correct lattice, then you can include the highest resolution available during the refinement. or One can pick fewer peaks during the peak search in hope that the ice ring will be

Re: [ccp4bb] Question re: ice rings in diffraction data

Dear Mona, If you used XDS to process the data you could use the EXCLUDE_RESOLUTION_RANGE keyword to exactly do what you describe. Best regards, Alexander Mit freundlichen Grüßen / Best regards / Cordialement Alexander Schiffer Sanofi-Aventis Deutschland GmbH RD CAS Structural Biology

Re: [ccp4bb] Question re: ice rings in diffraction data

Look at the reject fraction keyword and increase the value, as described on pp 39-40 of the manual Pete From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Mona Rahman Sent: Tuesday, July 15, 2008 7:14 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb]

Re: [ccp4bb] PST in refinement

Hi Maruf, 1) Why the low-resolution data was collected to 3.2 Å? 2) Is it possible the real space group is P2 with a NCS which making it look like P21, especially when the PST has a value of exact 0.5. Lijun On Jul 14, 2008, at 5:42 AM, Maruf Ali wrote: Dear all I have recently

[ccp4bb] obtaining hkl2map

Hi, I am trying to reach Thomas Schneider [EMAIL PROTECTED] to request a copy of hkl2map and his email server keeps on bouncing my message. Would somebody, by chance, have more recent contact info for him or have another suggestion on obtaining this program? Much appreciate your help,

Re: [ccp4bb] obtaining hkl2map

Thank to all the responders! I successfully obtained the program. Now all that's left is solving structures :). Cheers and thanks again, Vlad

Re: [ccp4bb] Question re: ice rings in diffraction data

Hi You can exclude the ice rings easily in Mosflm - from the command line - (a) Autoindexing - AUTOINDEX EXCLUDE ICE (but see user guide for more on this) (b) Processing - RESOLUTION EXCLUDE ICE Or if you try iMosflm, just check the button that says exclude ice rings

Re: [ccp4bb] Question re: ice rings in diffraction data

Dear Mona, Yes. You can get Denzo to reject reflections on or close to ice rings, or any other features with highly irregular background variations, by increasing the value of the REJECT keyword (see p. 40 of the HKL2000 manual by D. Gerwith,

[ccp4bb] Coiled Coils

Hi all, I was wondering if anyone knows a good website/software for prediction of coiled coil regions within proteins based on the amino acid sequence. Also does anyone know of examples where coiled coil regions can cause secondary structural changes by influencing the oligomeric state

Re: [ccp4bb] Coiled Coils

How about Coils ? http://www.ch.embnet.org/software/COILS_form.html jürgen On 15 Jul 2008, at 14:38, Neeraj wrote: Hi all, I was wondering if anyone knows a good website/software for prediction of coiled coil regions within proteins based on the amino acid sequence. Also does anyone