Should help if you use detergents and, of course, they are cheaper.
On Wed, 2009-04-29 at 15:38 -0400, Sang Hoon Joo wrote:
I sometimes notice people using non-siliconized cover glass and it
makes me wonder what pros and cons we can think about using
siliconized glass... any suggestions?
I have noticed that a significant majority of crystallizations are done in
hanging- rather than sitting-drop configuration, and considering the
significant extra labor involved in hanging drops, can only understand this
preference as a historical bias. I understand that sometimes one technique
We often find results to be very different between hanging and sitting
drops (equilibration kinetics for one may be the explanation). Then
there's the good thing of hanging drops that crystals rarely stick to
the surface of the support facilitating the mounting procedure, in
particular for
Drops tend to spread and flatten on non-siliconized cover glass, which is why
people use siliconized cover slides despite the cost. We recently find some
places sell siliconized cover slides at much lower prices, These are not as
fancy as the Hampton ones in terms of packaging, but worked
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There is really not much difference in terms of setup between hanging and
sitting drop, especially if the drops are set up on tape. Visualization is
also usually easier with hanging drops.
Bostjan
On 1/05/09 1:37 AM, Poul Nissen p...@mb.au.dk wrote:
We often find results to be very different
Dear Bernhard and all others who asked for this information:
The place we order siliconized cover slides:
Atlantic SciTech Group, Inc.
601 E. Linden Ave.
Linden, NJ 07036, USA
Phone: Tel. 800-720-9483; Fax 877-765-6616
E-mail: sa...@astginc.com
www.astginc.com
lin
- Original Message -
Hi, I beg to differ. Evaporation rates change dramatically between a
drop lying on a flat surface open to air flow; a drop lying in a
depression; and a drop lying in a depression with some reservoir
solution next to it. We're talking seconds to minutes.
So especially when using robotics
The (Australian) National Deuteration Facility has issued a call for
proposals for protein and DNA deuteration services. The deadline for
this first call is May 8, 2009.
Deuteration may be necessary or desirable for:
* Neutron crystallography
* Neutron scattering (e.g. SANS, neutron
Hi,
From the experiance of mine I can tell you that the crystal size sometimes
matters between these two methods. Hanging drop may yield bigger crystals than
sitting drop, that may be due to the evaporation rate(surface area). Hanging
drop allow us to set different protocols also like free
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