Deal all,
Sorry for the non-ccp4 query.
I am new to this field and need some suggestions regarding the improvement
in the crystallization qual
I have crystallized a protein with 65% MPD in 0.1M Acetate buffer pH 3.6 at
4 degree. The florets appear after 2 days and covers the whole drop. What
Heng--
Two things you might want to try:
1. Drop ratios with your current conditions and DMSO additive. Use
different concentrations of DMSO too.
2. Try using Hampton Crystal Screen and Crystal Screen 2 as additives. I
generally do this by adding 5% of XS 1 2 to the wells. Once I find
Dear,
I apologize for the non-ccp4 question.
I'm looking for an (alternative) modeling tool for DNA triple
helices, such as the earlier Morcad program:
MORCAD, and object-oriented molecular modelling package running on
IBM RS/6000 and SGI 4Dxxx workstations
M. Le Bret, J. Gabarro-Arpa,
Re: 65% MPD in 0.1M Acetate buffer pH 3.6
These conditions can crystallize salt impurities present in the protein
buffer.
The first step is to ensure that the crystals are protein.
If they are protein the next step is to reduce the MPD concentration and
seed
the drops if no crystals appear.
BEAM TIME AVAILABLE FOR MACROMOLECULAR CRYSTALLOGRAPHY AT THE TWO ID
BEAMLINES OF NE-CAT AT APS.
Beamline 24ID-C: Variable energy between 6-18keV, ADSC Q315 Detector, MAD
Capable.
Beamline 24ID-E: Equipped with MD2 microdiffractometer, delivering beam as
small as 5 microns. Single energy
Hey Folks,
I'm trying to refine a full capsid in REFMAC with about 25 atoms. I
haven't been successful and I suspect it's due to the MAXLINKS parameter in
atom_com.fh.
This is what I observe in the refmac log:
WARNING: number of links limit.
change parameter MAXLINK in
