The R factor and Rfree will always diverge during refinement; the model
is being modified to fit the working set, and not theFree set..
A largish difference at such a resolution is not surprising really.
Your rebuilding has improved the FreeR but it isnt surprising thatit has
not fallen
You need to try both P61 22 and P6522 with the Se sites changed fron
x,y,z to -x,-y,-z. The anom differences can be explained by either
solution. But maybe these programs automatically check both hands?
Eleanor Dodson
Ethayathulla Abdulsamath wrote:
hi BertThank you for your reply. Your are
Hi Christian
We often use a simple approach where people design their experiments using
rational multivariate design, but then simply use the best well that is found
as the centre of the next round of experiments. This means that you don't have
to score all the wells - which is
If you haven't already used it, I would certainly try the approach of
microseeding into screens since you already have crystals
http://scripts.iucr.org/cgi-bin/paper?S0907444907007652
--
patr...@douglas.co.uk mailto:patr...@douglas.co.uk Douglas
Instruments Ltd.
DouglasHouse,
Dear all,
I am testing Mr BUMP on vista and run into the following error message,
after it has performed fasta and clustal alignements, it downloads
several pdb files such as:
PDB Download log: Chain:1qyc_B
Download Log: Attempting to download the
Dear all,
here is a summary of the few responses that I have got from my posting
on MALLS equipment suggestions.
2 labs have WYATT miniDawn system. One is connected to an Agilent HPLC.
Seems to be quite good.
1 lab has a Waters equipment connected to an Akta FPLC, which seems to
be more
Dear Friends,
I have Zn atoms in my pdb file. So, I think I need to run
elbow to create the cif otherwise refinement stops.
I did the following and got the error as follows:
phenix.elbow generate_easy_r4.pdb --do-all
Hey,
I have a GST fusion protein secreted into express5 (with hint of serum),
it does not bind beads. Changing pH with 4xPBS limits ppt and enables
binding but in a scale up I think it makes sense to use a TFF/CFF setup
for concentration and diafiltration.
Does anybody have advice about
Hi Drew,
Sorry for the slightly late reply. Something that 'might' be able to help
(though I fully agree with Dale's reply) is the procedure for local density
improvement described here:
Acta Cryst. (1997). D53, 540-543 Local Improvement of Electron-Density Maps.
We are currently in the
