Hi Xuan,
I guess your protein is not an E.coli protein. There are several
examples that eukaryotic Zn-proteins expressed in E.coli contain Fe
instead of Zn. I am sceptic whether IMAC with different metal ions will
give the solution of the problem. If you really want to get information
on the
Vesna Serrano wrote:
Dear all,
I am using Refmac_5.5.0088 in CCP4 6.1.0, linux version. The program runs
well normally, but all of a sudden it failed with the following message:
Refmac_5.5.0088: Open failed: File: /tmp/vesna/refmac5_temp1.06489.txt.
Here is the log file of the run:
#CCP4I
Since you sent your question to CCP4bb you presumably have crystals!
In that case an excellent way to check which metal atoms you have in
which sites is to collect datasets at a synchrotron at suitably
chosen wavelengths and look at the anomalous maps. For an example
see Acta Cryst. D62 (2006)
Dear CCP4BBer,
I'm using Phaser 2.1.4 to solve a structure containing both protein and DNA.
The packing function works find with the protein, but does not recognize the
trace of the DNA. Does anyone know precisely what should be the pdb format ?
Here is the format that I'am using :
ATOM 1 P
Hi,
Thanks a lot for the replies. It seems that Randy¹s solution works fine.
Marie-Ln
Le 6/08/09 13:54, « Randy Read » rj...@cam.ac.uk a écrit :
Hi,
That's actually the last question answered on our FAQ page!
http://www-structmed.cimr.cam.ac.uk/phaser/faq.html
Basically, you'll want
Dear Mr. Fritz,
Yes, the protein is not an E.coli protein! Instead, it was cloned from a
virus. And since it was a nonstructural viral protein, I thought it might be
appropiate to treat it as eukaryotic proteins.
E.coli system was quite different from eukaryotic ones, hence I was quite
cautious
Long time ago I had a superoxide dismutase that was
active with iron as well as manganese. No matter what
functional metal (Fe or Mn) was bound a
substantial fraction up to 1/3 of the molecules
had the (non-functional) Zinc in their metal
binding site (found by AAS and EXAFS).
Zinc is
Protein Production Senior Scientist
Job Description:
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As others have implied, quantifying metals in
metalloproteins is very challenging. In my experience, the principal
problems are (1) adventitious metal contamination, (2) accurate
measurement of protein concentration, and (3) weak metal binding.
Zinc and iron are ubiquitous microcontaminants,
Sorry, I meant to say does divergence add to the reported mosaicity
value. If so, do actual mosaicity and divergence add in quadrature
to give the reported value?
On Aug 6, 2009, at 6:14 PM, Richard Gillilan wrote:
Does anyone know if beam divergence gets included in the mosaicity
values
Richard Gillilan wrote:
Sorry, I meant to say does divergence add to the reported mosaicity
value. If so, do actual mosaicity and divergence add in quadrature to
give the reported value?
Not sure what denzo does, but in general if you convolute two things
that have a Gaussian distribution,
Richard Gillilan wrote:
Sorry, I meant to say does divergence add to the reported mosaicity
value. If so, do actual mosaicity and divergence add in quadrature to
give the reported value?
Yes, they do add in quadrature, the total is reported by scalepack and
HKL2000 (if postrefinement is
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