If I recall correctly, your best option was to guess as to what could
be going on at the bottom of the window when you found that your
screen resolution was not up to the standards set by HKL Research. Of
course, I'm remembering from 2001. Under most circumstances one
expects software
I have custom O stereochemistry files that make tweaking DNA very
easy--if you know how to use O.
These days I think it is more stylish to use coot, however. I've never
used it for serious work, but I also think coot has strange and
wonderful abilities that renders the discrimination of
Next MX-proposal application deadline: September 1, 2009
See:
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We kindly request new MX-proposals for beamtime applications for the next
beamtime period.
In order to apply for beamtime, please register at the BESSY on-line
access tool BOAT
Dear All,
I am little curious while refining my structure with refmac5.
Each refinement of refmac produces two main files one mtz and one pdb. Will
there be any difference with taking the latest mtz vs taking the oldest mtz
with the latest pdb.
Thank you for your help.
Sincerely
Couple of things, Ru Heng.
1. What buffer conditions is your protein in? Is it similar to the
buffer you describe as using to dissolve your DNA in? In general, you
can even get away with dissolving and annealing the oligos in just
Tris etc.
2. Play with buffer conditions, particularly
Ru Heng,
It is commonly helpful to combine your protein and DNA under
dilute conditions and then concentrate the complex. Combining
concentrated DNA and protein together has a very good chance of
precipitating in my experience. I completely agree that trying
different buffer
Debajyoti Dutta wrote:
Dear All,
I am little curious while refining my structure with refmac5.
Each refinement of refmac produces two main files one mtz and one pdb. Will
there be any difference with taking the latest mtz vs taking the oldest mtz
with the latest pdb.
Thank you for your
Please come to
Structural Biology Symposium to mark the retirement of Gordon Roberts
Wednesday 23rd September 2009, 10 am – 6pm
Department of Biochemistry, University of Leicester
Confirmed Speakers: Martin Blackledge (IBS, Grenoble), Jim Feeney (NIMR), Ben
Goult (Leicester), Lu-Yun
Hi ruheng,
Since you synthesized the oligos, you probably already know if there is
any residual salt or buffer in your oligos. I don't know if that
caused the problem. Sometimes people purify and desalt the oligos
before the annealing step.
Joe
ruheng wrote:
Dear CCP4bbers,
I am now
If you have a spare monitor you can always set up your macbook to use multiple
screens. Once connecting your spare, go into system preferences, displays, and
there you can manipulate the screens to be side-by-side.
Regina
--- On Wed, 8/12/09, Putcha, Balananda Dhurjati Kumar bput...@utk.edu
Hi All,
Does anyone know of a program that can extract the amino sequence of a
protein from a PDB file and output it as a FASTA file?
Thanks! and all the best,
--Buz
Thanks Mark!
--buz
On Aug 13, 2009, at 5:45 PM, Dr. Mark Mayer wrote:
try PDBSET (ccp4)
Hi All,
Does anyone know of a program that can extract the amino sequence
of a protein from a PDB file and output it as a FASTA file?
Thanks! and all the best,
--Buz
--
Hi Buz,
phenix.print_sequence model.pdb
will print sequence from input PDB file (although I don't know if it is
a FASTA format).
Pavel.
On 8/13/09 2:34 PM, Buz Barstow wrote:
Hi All,
Does anyone know of a program that can extract the amino sequence of a
protein from a PDB file and
Hi Buz,
If you have Phenix, you can use the phenix.print_sequence tool to output
the sequence in FASTA format. I believe MOLEMAN (
http://xray.bmc.uu.se/usf/xutil.html) can also perform similar function.
My best,
Jack
On Thu, Aug 13, 2009 at 2:34 PM, Buz Barstow b...@mac.com wrote:
Hi All,
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