Dear all,
does anyone know of a programme that would allow the graphical
representation of the packing of protein molecules in a crystal?
Ideally one would like to represent the protein as an elipsoid (or
similar relatively simple shape) corresponding to its volume, over a
few unit cells
Hi,
Pymol may do this work, useAction--generate--symmetry mates
-- Original --
From: Anita Lewit-Bentleyale...@pasteur.fr;
Date: Wed, Aug 26, 2009 05:48 PM
To: CCP4BBCCP4BB@JISCMAIL.AC.UK;
Subject: [ccp4bb] packing diagrammes
Dear all,
The cns script neighbours.inp might be useful for this purpose.
From: AntonioLeung leun...@live.cn
Reply-To: AntonioLeung leun...@live.cn
Date: Wed, 26 Aug 2009 21:46:26 +0800
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] packing diagrammes
Hi,
Pymol may do this work,
The PDF files of the talks from the workshop on macromolecular
twinning at the 2009 ACA Meeting in Toronto may now be dowloaded
free from the ACA web site: http://www.amercrystalassn.org
George
Prof. George M. Sheldrick FRS
Dept. Structural Chemistry,
University of Goettingen,
Tammannstr. 4,
Hi
Does anyone know how to configure ArpWARP manually so that it will be
recognized by the latest version of ccp4?
Best regards
Sylvia Fanucchi Ph.D
Protein Structure-Function Research Unit
East Campus, Gate House Room 416
School of Molecular and Cell Biology
University of the
Hi all
I'm trying SIR on my crystal both are C222.
Native
Cell Dimensions: 97.66 243.45 87.62 90 90 90
Resolution: 4.3
Derivative
Cell Dimensions: 96.9100 244.66 87.61 90 90 90
Resolution: 4.7
. I plan to scale together using FHScal. Ctruncate says that my
derivative has strong
Hi everyone,
I am trying to run MrBUMP through the CCP4 6.1.1 but the program is
complaining that no multiple alignment programs like mafft, clustalw,
clustalw2, probcons, or t_coffee were found on the system.
Will I need to install all these programs and will they need to be in the
CCP4
Hi All,
After transferring a lot of files from one computer to another, we
discovered a problem when we launched ccp4i. The project database
job list is empty even though all the .log files, etc. are clearly
present. We checked the CCP4_DATABASE directory and discovered that
the import.def
Dear all,
This is a question on how to cope with the protein that seems to form
massive aggregates in solution but enzymatically active.
I'm working on a protein whose molecular weight is around 70kDa and can be
divided into two domains (say A and B domains). We expressed this protein in
You could try adding detergents. We had a case a few years ago of an enzyme
that was highly active but also highly aggregated. Addition of n-octyl
beta-d-glucopyranoside significantly lowered the degree of aggregation such
that crystals could be grown. Lowering the protein concentration also
Dear Ose,
This is unfortunately a fairly common issue. There are multiple ways to
rationalize what's happening - and even more approaches to reducing the
aggregation issues. It would take too long to list all the options here -
however I would look at the earliest stages of your expression and
Well, this is of course just my undereducated two cents but the first
question I'd ask is: asuming that dataset #2 is a real derivative - can I
find realistic sites? What are you basing your resolution cutoff on - in the
diffraction images, do you visually observe anisotropy?
It's not uncommon
Just try crystallizing it. What is a crystal but a massive
aggregate? That they are still soluble and active is great news.
As a grad student, I had a similar phenomenon with an early project. I
showed a gel in group meeting where both activity and aggregation were
obvious, said the
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