Dear bb,
we actually solved quite a lot of structure with S-SAD on in-house systems
with Cu radiation. Of course it is very difficult to give absolute numbers in
term of S atoms per number of residues since the success of a SAD experiment
strongly depends on the quality of the crystal and the
Dear Users,
The deadline for October/November/December 2009 Collaborative
Crystallography proposals will be *November 15, 2009. *
Through the Collaborative Crystallography Program (CC) at the
Advanced Light Source (ALS),
Hi All
Sorry that this is a non-ccp4 question, but I hope I can get some good
suggestions from the community.
We have protein crystals under various conditons and want to soak them
with different potential inhibitors. Most of inhibitors have very small
molecular weights (200-300), so it
Dear Users,
The deadline for Jan/Feb 2010 Collaborative
Crystallography proposals will be *November 15, 2009. *
Through the Collaborative Crystallography Program (CC) at the
Advanced Light Source (ALS), scientists can send
Hello Jan,
I stored intact IgGs as an ammonium sulfate cut at 4C with a little
azide for several months with no problems, but I can't recall ever
trying long-term storage of the Fabs. Good luck.
-Anna
On Fri, Nov 6, 2009 at 8:52 AM, Jan Rash jan...@googlemail.com wrote:
Dear All,
Does any
Rongjin,
(As you may know) there is considerable precedent for soaking mixtures of small
fragments into crystals. If you choose compounds to be shaped differently, you
can determine which compound is bound by the shape of the electron density map
for the ligand. I would not be concerned about
Hi,
1. You can learn about compound binding using methods orthogonal to X-ray
(i.e. SPR, NMR, and so forth). If the sole detection method available to you
is X-ray then you should expect to need a reasonably complete dataset in
order to determine binding - otherwise you can easily miss binding
Too many reflections to store
These errors are so 1993.
ROFLMAO.
sayre 31% wc my_structure.sca
1809450 9047245 52474047 my_structure.sca
sayre 32% python
Python 2.4.5 (#1, Sep 18 2008, 14:53:26)
[GCC 3.2 20020903 (Red Hat Linux 8.0 3.2-7)] on linux2
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