Dear CCP4ers,
on behalf of Patrick Cramer, I would like to draw your attention to the
following open position at the Gene Center, University of Munich.
Please, do not reply directly to me, but to the e-mail addresses given
at the end of this job offer.
Best regards,
Dirk Kostrewa.
---
Hi folks,
I'm having a problem with Coot. Molecular Replacement solution to the
phase problem. The MR model has one small stretch of residues missing,
for which there is density in the maps (both normal and difference
density maps). Hence I have built the model into this density. Except
that
You can renumber the residues within COOT.
Calculate Renumber Residue
Input the residue numbers you wish to change, then by how many you want to
offset the sequence.
I do not believe you should stick with 'standard numbering schemes'. It
would probably be best (and make your refinement's
Dear all,
I do have a question about comparing Rfree and Rwork factors of different
refinement trials whereas I always started with the same pdb file and structure
factors (phasing by MR).
Means I had a protein structure which was (not just by me) refined several
times in different ways also
Dear Jan,
an initial increase in Rfree usually means, that either, in case of
isomorphous crystal forms, the structure was refined before against a
different test set, or, in case of completely different crystal forms,
that you have a new test set. In both cases, Rwork and Rfree for the
Dear all,
We recently jump from a local version of ccp4 using Refmac_5.2.0019 to
an up-to-date version of ccp4 distributed by sbgrid using
Refmac_5.2.0102. From then, refinements with alternate configurations
are blowing-up in refmac. A similar problem was solved thanks to
ccp4bb ('Refmac
We have had dozens of boxes of XtalQuest 42-2 plates sitting on the
shelf for three years and I've just been told we've only sold three
boxes so far
We'll accept any reasonable offers!
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Patrick
EMBO Practical Course - PEPC7
Protein Expression, Purification and Crystallisation
EMBL Hamburg, Germany
Monday 23 August - Tuesday 31 August 2010
The course, intended for students in the early stages of their research
career, will cover over-expression of proteins in prokaryotic and
Dear All,
I have received the following suggestion from a crystallographer. The
bottom part of the email was indicating that the mail was confidential,
so I have removed the name of the author of that email (together with
this section about confidentiality) and I am therefore forwarding the
would ssm serve your purpose?
eleanor
ebi or ccp4i
Miri Hirshberg wrote:
Sun., Jan. 17th 2010
EBI
Greetings,
I am looking for a 3D structure superposition program which takes
two structures and superpose them based only on the coordinates X,Y,Z
regardless of of residue/atoms name.
(both
A number of post-doctoral positions are available in the group of Marko
Hyvonen, at the Department of Biochemistry, University of Cambridge.
These positions are in multidisciplinary projects aimed at developing
novel therapeutic agents and chemical tools against protein-protein
interactions
Dear all,
I am refining a structure of a protein in a complex with phosphorylated
ligand (inositol ring). My problem is that after restrained refinement with
Refmac, the ring is distorted in geometrically impossible ways. Could
anybody advise me on how to deal with this problem? Ideally, I
Hi,
I'm using Sketcher to create the following:
C=C C=C
/ \ / \
C CC
The problem is that I can't figure out how to keep the carbons cis. I see
the Add Row button in Sketcher and I can specify the Sign. There are
also spaces to fill in B/3 F/4 1/5 2/6
Dear all,
I just stumbled across the question about what is the unit of f' and f''. The
first couple of hits from ixquick.com claim it was e^-. Since e^- is not a unit
but symbolises an elemtary particle (of which fractions are considered
non-existent), I was wondering whether the unit of f, f',
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