Dear Bill,
as I pointed out in my previous post I have the impression that using wish
(instead of bltwish) libblt provided in Debian stable now works fine with ccp4,
including loggraph etc.
So it might be too late for a complaint, although my statement is based only on
a first impression rather
Dear Katherine,
I would like to add a futher point to this discussion: If you do model residues
with poor or no electron density, then you should be sure of what you are doing
and not just make more or less unguided guesses. This seems obvious but
unfortunately it isn't, at least not to
On Apr 15, 2010, at 10:08, Thomas Lütteke wrote:
Dear Katherine,
I would like to add a futher point to this discussion: If you do
model residues with poor or no electron density, then you should be
sure of what you are doing and not just make more or less unguided
guesses. This seems
Hi -
My two cents:
First, you say:
I assume the bigger crystal might have lot of solvent which prevent
for high resolution. If it is true what could be the best way to
dehydrate crystal without affecting crystal quality?
I think this assumption is confusing. If the crystals were grown in
Dear All,
I have a question about the use of glutaraldehyde as a cross-linking
agent.
It is a practical question. The glutaraldehyde you can buy from Sigma-
Aldrich
contains a mixture of polymers according to the product information
with an increasing
number of polymers with increasing pH.
and don't forget to check diffraction without freezing.
Mark
On 15 April 2010 10:37, Anastassis Perrakis a.perra...@nki.nl wrote:
Hi -
My two cents:
First, you say:
I assume the bigger crystal might have lot of solvent which prevent for
high resolution. If it is true what could be the
Sorry - I had not twigged that this was a SAD discussion.
In this case DM is as you say gonna save you from bimodal ambiguities - as we
all know that is why the FOMs get so much better during DM - but that seems
fine to me as it is pretty much new information coming in from the solvent
Ensure that these lines are somewhere in your
xorg.conf file:
# -
Section "Module"
Load "glx"
EndSection
Section "Device"
Identifier "Configured Video Device"
Driver "nvidia"
Option "Stereo" "3"
EndSection
Section "Extensions"
Option "Composite" "Disable"
EndSection
Does anyone have a good estimate of the proportion of molecular
replacement structures in the PDB?
I tried using the search tools at RCSB and PDBe. The PDBe gives 55.1%
but apparently only 3411 structures have phase determination information
recorded, so this does not look to be terribly
You've gotten some helpful replies already. I have found the following
reference to be helpful in understanding some of the physics behind
damage incurred during the crystal cooling process and a general
strategy to help avoid it. It expands upon what's already been said -
that larger
General announcement and how to apply: http://www.farma.ku.dk/index.php?id=8206
Project description: http://www.farma.ku.dk/index.php/Project-6/8214/0/
Deadline for applications: Monday 3 May 2010 at 12 o'clock noon
About the group: http://www.farma.ku.dk/BR/
Please contact me if you wish
The biannual Gordon Conference on Diffraction Methods in Structural
Biology will be held at Bates College in Lewiston, Maine from 18-23rd
July. The program covers all aspects of macromolecular structure
solution from crystallization to structure solution and refinement and
includes
Dear Colleagues,
I am very grateful to everyone who contributed to the discussion regarding
phasing statistics that I initiated. I certainly found it very informative.
Below is a summary of the technical responses that I regarding this problem.
1) Use some of the statistics that SHELXD and
Dear All,
Attached is information about a new X-ray crystallography training program
being introduced at Pennsylvania State University.
Deadline for registering is May 1st 2010. Kindly pass it along to anybody that
may be interested in such an opportunity. Thank you
Neela
attachment:
Hi
Not a question about films for recording X-rays on, but a question
about films about X-rays, Crystallography and related subjects!
I was wondering what ccp4bbers favourite movies involving real
science, especially crystallography might be? If they're from
Hollywood, though, I'd guess
Both the original movie and the made-for-TV movies of The Andromeda Strain
had short references to crystallography, the first in how the strain was
organized, the second in how a message from the future was encoded in
buckyball nanotechnology crystal.
Perhaps this is why they are my favorite
Hi Harry-
X-ray crystallography played an integral part in discoveries made in Michael
Crichton's Andromeda Strain. Mainly it was used to determine the elemental
composition and arrangement of the capsid or shell that the foreign
organism was found within.
Best-
Brad
On Thu, Apr 15, 2010 at
Hi Amit-
We used a well-expressing soluble protein fusion (SUMO) at the N-term of a
couple of B. anthracis proteins that we were trying to produce in E. coli,
which improved the soluble yield of both targets tremendously. The
disadvantage of a fusion is of course you produce a non-native protein-
Hi Folks,
Is there an easy way to generate a composite omit map using CCP4? I know
this has been discussed a few times before. But has anybody written some
new programs recently?
Regards,
Weikai
The documentary Naturally Obsessed: The Making of a Scientist is definitely a
must-see film. It captures the story of life and science in a crystallography
lab, that of Dr. Larry Shapiro at Columbia University, and follows the graduate
students journey of fortune and misfortune that
Hi all,
I am highly thankful to all of you for very useful replies.
The following are the list of the replies to the query.
Query:
Hi all,
Sorry for a non-ccp4 query.
I will highly appreciate your comments on the expression:
I am trying to express HIS tagged ~60 kDa cytosolic protein from
Wrath
of
Khan
On Thu, Apr 15, 2010 at 11:42 AM, Chayne Piscitelli pisci...@ohsu.edu wrote:
The documentary Naturally Obsessed: The Making of a Scientist
is definitely a must-see film. It captures the story of life and
science in a crystallography lab, that of Dr. Larry Shapiro at Columbia
Well, the original movie was reasonably well done. But the made for TV
version is deeply rewarding, for all the wrong reasons, being so bad, it's
good.
SPOILER ALERT: Read no further if you want to truly enjoy the the silliest
four minutes in the history of made-for-television movies,
Terminator 3 - IIRC, a synchrotron strips the fluid-metal exoskeleton off of
TX - that counts, right?
--
Delivered via an Android.
On Apr 15, 2010 9:07 PM, Brock Schuman bro...@gmail.com wrote:
Wrath
of
Khan
On Thu, Apr 15, 2010 at 11:42 AM, Chayne Piscitelli pisci...@ohsu.edu
wrote: The
POSTDOCTORAL POSITION IN PROTEIN CRYSTALLOGRAPHY AT EMORY UNIVERSITY
A postdoctoral position is available in the Department of Biochemistry at Emory
University (Atlanta, GA). This position has been funded for two years to
support an NIH R01 grant aimed at understanding the structural and
The Race for the Double Helix (2004)
http://www.amazon.com/Race-Double-Helix-VHS-Pigott-Smith/dp/6303247911/ref=sr_1_1?ie=UTF8s=dvdqid=1271365899sr=1-1
When crystallography was on FILMS not CCD's:)
Eva Kirchner wrote:
favourite movies involving real science
The Dark Crystal (1982)!
I'm not sure there was actually any science in the movie, but 'The Incredible
Hulk' had some opening scenes shot at the beautiful ALS synchrotron... and I
just want to point out that I only saw that movie because my kids made me!
Took a lot of junk food to make it through that one
-Robyn
Mike Carson claims that the spinning DNA in the computer monitor in
Jurassic Park was the product of the Ribbons software package:
http://www.cbse.uab.edu/carson/
James
On Apr 15, 2010, at 9:16 AM, harry powell wrote:
Hi
Not a question about films for recording X-rays on, but a
Thanks everyone for letting me know its canister. I had a different idea of
a canister. I really appreciate all your replies.Instead of replying
individually, I am sending this common email.
Thanks again,
Ivan
(What is in the Hollywood district other than tourists looking for
Hollywood?)
I have a silly anecdote that does not answer Harry's question:
It must have been 1995 or 1996, the Beckman sales representative
appeared at my desk on a Friday afternoon without an appointment, but
carrying a
Did I see a PyMol screenshot (or something very like it) at the
beginning of Avatar?
James Stroud wrote:
Mike Carson claims that the spinning DNA in the computer monitor in
Jurassic Park was the product of the Ribbons software package:
http://www.cbse.uab.edu/carson/
Right you are, Chayne,
I think it should be shown to every Protein crystallographer graduate
student on their first day in the lab.
ivan
On Thu, Apr 15, 2010 at 11:42 AM, Chayne Piscitelli pisci...@ohsu.eduwrote:
The documentary Naturally Obsessed: The Making of a Scientist
is definitely a
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